Restriction map for
pFN21AE4308
(Circular) (Six-base) MAPSORT of: pFN21AE4308.seq Check: 9106 from: 1 to: 5659
pFN21AE4308 5659 bp
With 182 enzymes: SgfI *
March 28, 2012 14:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2955 3002 1937
Size: 1018 47 4594
Fragments arranged by size:
4594 1018 47
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3722 5106 5374 1632
Size: 2090 1384 268 1917
Fragments arranged by size:
2090 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2074 3402 4974 2074
Size: 1328 1572 2759
Fragments arranged by size:
2759 1572 1328
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2540 2968 3194 3346 5455 1493
Size: 1047 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 1047 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2379 2466 2984 1870
Size: 73 436 87 518 4545
Fragments arranged by size:
4545 518 436 87 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2127 2980 4435 618
Size: 325 217 627 240 100 853 1455 1842
Fragments arranged by size:
1842 1455 853 627 325 240 217 100
BanII G_rGCy'C
Cuts at: 1391 2978 1391
Size: 1587 4072
BbeI G_GCGC'C
Cuts at: 2131 2131
Size: 5659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 928
Size: 971 190 4498
Fragments arranged by size:
4498 971 190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2589 3551 4761 2589
Size: 962 1210 3487
Fragments arranged by size:
3487 1210 962
BclI T'GATC_A
Cuts at: 1548 2478 1548
Size: 930 4729
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2681 2705 4270
Size: 107 122 71 631 605 1009 24 1565
Cuts at: 4270 136
Size: 1525
Fragments arranged by size:
1565 1525 1009 631 605 122 107 71
24
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2686 3720 4876 1790
Size: 896 1034 1156 2573
Fragments arranged by size:
2573 1156 1034 896
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2570 2669 2678 2828 4319 1239
Size: 1331 99 9 150 1491 2579
Fragments arranged by size:
2579 1491 1331 150 99 9
Bpu10I CC'TnA_GC
Cuts at: 2057 2057
Size: 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 2483 5653 2483
Size: 3170 2489
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2128 3466 3848 4483
Size: 53 83 186 936 595 1338 382 635
Cuts at: 4483 275
Size: 1451
Fragments arranged by size:
1451 1338 936 635 595 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 1178
Size: 30 165 30 1116 30 4288
Fragments arranged by size:
4288 1116 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2212 1186
Size: 1026 4633
BseYI C'CCAG_C
Cuts at: 2023 2679 4862 5358 2023
Size: 656 2183 496 2324
Fragments arranged by size:
2324 2183 656 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2922 3720 3805 4876 2922
Size: 798 85 1071 3705
Fragments arranged by size:
3705 1071 798 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2686 2922 2978 3720 3805 4876 1391
Size: 399 896 236 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 896 742 399 236 85 56
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 2760 3548 5278 2760
Size: 788 1730 3141
Fragments arranged by size:
3141 1730 788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2901 3014 844
Size: 1370 687 113 3489
Fragments arranged by size:
3489 1370 687 113
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2092 4154 4328 65
Size: 1183 844 2062 174 1396
Fragments arranged by size:
2062 1396 1183 844 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 2105 4303 4490 1700
Size: 110 254 41 2198 187 2869
Fragments arranged by size:
2869 2198 254 187 110 41
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 794
Size: 1280 4379
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 2431 1184
Size: 1247 4412
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2290 2497 2908 2245
Size: 45 207 411 4996
Fragments arranged by size:
4996 411 207 45
BstYI r'GATC_y
Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654
Size: 1017 367 385 17 1452 86 12 346
Cuts at: 5654 1972
Size: 1977
Fragments arranged by size:
1977 1452 1017 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2570 2570
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 2369 513
Size: 552 1304 3803
Fragments arranged by size:
3803 1304 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 2138 3175 3969 3989 2049
Size: 89 1037 794 20 3719
Fragments arranged by size:
3719 1037 794 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 3106 3995
Size: 54 1425 50 275 18 342 934 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 934 889 342 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 3589 1353
Size: 669 1567 3423
Fragments arranged by size:
3423 1567 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2570 2669 2678 2828 3722 4319 5106
Size: 393 938 99 9 150 894 597 787
Cuts at: 5106 5374 1239
Size: 268 1524
Fragments arranged by size:
1524 938 894 787 597 393 268 150
99 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2789 2789
Size: 5659
EcoO109I rG'GnC_Cy
Cuts at: 2203 2420 2721 2929 2203
Size: 217 301 208 4933
Fragments arranged by size:
4933 301 217 208
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
EcoRV GAT'ATC
Cuts at: 2251 2251
Size: 5659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2333 2333
Size: 5659
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2131 4806 1678
Size: 453 2675 2531
Fragments arranged by size:
2675 2531 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2592 2624 4300 4332 4374
Size: 32 135 32 1020 32 1676 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 1676 1020 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KasI G'GCGC_C
Cuts at: 2127 2127
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 4773 4957 1305
Size: 921 2547 184 2007
Fragments arranged by size:
2547 2007 921 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 10
Size: 54 1425 343 342 3495
Fragments arranged by size:
3495 1425 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3617 3976 4135 518
Size: 648 2451 359 159 2042
Fragments arranged by size:
2451 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2178 2212 2395 3075 3752 4900 5145
Size: 540 32 34 183 680 677 1148 245
Cuts at: 5145 1606
Size: 2120
Fragments arranged by size:
2120 1148 680 677 540 245 183 34
32
NarI GG'CG_CC
Cuts at: 2128 2128
Size: 5659
NcoI C'CATG_G
Cuts at: 513 1065 2369 513
Size: 552 1304 3803
Fragments arranged by size:
3803 1304 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2816 3017 4562 1132
Size: 97 1587 201 1545 2229
Fragments arranged by size:
2229 1587 1545 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2370 1108
Size: 1262 4397
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2420 2929 2203
Size: 217 509 4933
Fragments arranged by size:
4933 509 217
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2145 2211 2460 2673 3011 830
Size: 1315 66 249 213 338 3478
Fragments arranged by size:
3478 1315 338 249 213 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2146 2178 2146
Size: 32 5627
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SanDI GG'GwC_CC
Cuts at: 2420 2420
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 2673 3011 2673
Size: 338 5321
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2141 2207 2456 2669 2845 3007 4142
Size: 219 1096 66 249 213 176 162 1135
Cuts at: 4142 4823 5014 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1096 681 249 219 213 191
176 162 66
SfoI GGC'GCC
Cuts at: 2129 2129
Size: 5659
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2381 2468 2986 1872
Size: 509 87 518 4545
Fragments arranged by size:
4545 518 509 87
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 2816 3017 1132
Size: 1684 201 3774
Fragments arranged by size:
3774 1684 201
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2555 2675 1517
Size: 1038 120 4501
Fragments arranged by size:
4501 1038 120
StyI C'CwwG_G
Cuts at: 513 1065 2369 2790 3098 513
Size: 552 1304 421 308 3074
Fragments arranged by size:
3074 1304 552 421 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2015 2278 3686 3703 3856 4041 4486
Size: 1255 435 263 1408 17 153 185 445
Cuts at: 4486 325
Size: 1498
Fragments arranged by size:
1498 1408 1255 445 435 263 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2404 2436 5619 5651 2404
Size: 32 3183 32 2412
Fragments arranged by size:
3183 2412 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2545 2545
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2379 2466 2984 1870
Size: 509 87 518 4545
Fragments arranged by size:
4545 518 509 87
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI