Restriction map for
pFN21AE4306
(Circular) (Six-base) MAPSORT of: pFN21AE4306.seq Check: 6972 from: 1 to: 5623
pFN21AE4306 5623 bp
With 182 enzymes: SgfI *
March 28, 2012 14:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3433 278
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2966 1937
Size: 1029 4594
Acc65I G'GTAC_C
Cuts at: 2944 2944
Size: 5623
AclI AA'CG_TT
Cuts at: 1563 3750 4123 1563
Size: 2187 373 3063
Fragments arranged by size:
3063 2187 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 2858 2904 3686 5070 5338 1632
Size: 1058 168 46 782 1384 268 1917
Fragments arranged by size:
1917 1384 1058 782 268 168 46
AflII C'TTAA_G
Cuts at: 820 1017 2149 820
Size: 197 1132 4294
Fragments arranged by size:
4294 1132 197
AflIII A'CryG_T
Cuts at: 1203 4522 5499 1203
Size: 3319 977 1327
Fragments arranged by size:
3319 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 4352 4352
Size: 5623
AleI CACnn'nnGTG
Cuts at: 1166 2481 1166
Size: 1315 4308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5623
AlwNI CAG_nnn'CTG
Cuts at: 2083 3366 4938 2083
Size: 1283 1572 2768
Fragments arranged by size:
2768 1572 1283
ApaLI G'TGCA_C
Cuts at: 3680 4836 3680
Size: 1156 4467
ApoI r'AATT_y
Cuts at: 1493 2196 2690 2932 3158 3310 5419 1493
Size: 703 494 242 226 152 2109 1697
Fragments arranged by size:
2109 1697 703 494 242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4177 160
Size: 872 3145 1606
Fragments arranged by size:
3145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2948 1870
Size: 73 124 881 4545
Fragments arranged by size:
4545 881 124 73
BamHI G'GATC_C
Cuts at: 2308 2953 3320 2308
Size: 645 367 4611
Fragments arranged by size:
4611 645 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2944 4399 618
Size: 325 217 627 308 849 1455 1842
Fragments arranged by size:
1842 1455 849 627 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2474 2942 1391
Size: 1083 468 4072
Fragments arranged by size:
4072 1083 468
BbeI G_GCGC'C
Cuts at: 2099 2099
Size: 5623
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 928
Size: 971 332 4320
Fragments arranged by size:
4320 971 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3814 3848 1903
Size: 34 1877 34 3678
Fragments arranged by size:
3678 1877 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3515 4725 3515
Size: 1210 4413
BclI T'GATC_A
Cuts at: 1548 2403 1548
Size: 855 4768
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4234 136
Size: 107 122 71 631 605 2562 1525
Fragments arranged by size:
2562 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5618 5618
Size: 5623
BlpI GC'TnA_GC
Cuts at: 3040 3040
Size: 5623
Bme1580I G_kGCm'C
Cuts at: 1790 3684 4840 1790
Size: 1894 1156 2573
Fragments arranged by size:
2573 1894 1156
BmgBI CAC'GTC
Cuts at: 1318 2242 1318
Size: 924 4699
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3354 4312 442
Size: 416 53 83 399 24 1937 958 1753
Fragments arranged by size:
1937 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4283 1239
Size: 3044 2579
Bpu10I CC'TnA_GC
Cuts at: 2304 2304
Size: 5623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4613 4911 3748
Size: 865 298 4460
Fragments arranged by size:
4460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2777 4286 882
Size: 617 1278 1509 2219
Fragments arranged by size:
2219 1509 1278 617
BsaAI yAC'GTr
Cuts at: 493 1204 3414 493
Size: 711 2210 2702
Fragments arranged by size:
2702 2210 711
BsaBI GATnn'nnATC
Cuts at: 2586 2619 5617 2586
Size: 33 2998 2592
Fragments arranged by size:
2998 2592 33
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2096 3430 3812
Size: 53 83 186 936 476 87 1334 382
Cuts at: 3812 4447 275
Size: 635 1451
Fragments arranged by size:
1451 1334 936 635 476 382 186 87
83 53
BsaWI w'CCGG_w
Cuts at: 4056 4454 4728 4875 5286 4056
Size: 398 274 147 411 4393
Fragments arranged by size:
4393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2600 1178
Size: 30 165 30 1167 30 4201
Fragments arranged by size:
4201 1167 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5623
BseYI C'CCAG_C
Cuts at: 2510 4826 5322 2510
Size: 2316 496 2811
Fragments arranged by size:
2811 2316 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5050
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3834 4862 1540
Size: 275 186 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2474 3684 3769 4840 2474
Size: 1210 85 1071 3257
Fragments arranged by size:
3257 1210 1071 85
BsmI GAATG_Cn'
Cuts at: 2512 3134 3227 2512
Size: 622 93 4908
Fragments arranged by size:
4908 622 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2474 2942 3684 3769 4840 1391
Size: 399 684 468 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 468 399 85
BspEI T'CCGG_A
Cuts at: 5286 5286
Size: 5623
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2066 2978 844
Size: 1222 912 3489
Fragments arranged by size:
3489 1222 912
BsrBI CCG'CTC
Cuts at: 1394 1823 2629 3510 1394
Size: 429 806 881 3507
Fragments arranged by size:
3507 881 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4118 4292 65
Size: 1183 2870 174 1396
Fragments arranged by size:
2870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 4267 4454 1700
Size: 110 282 2175 187 2869
Fragments arranged by size:
2869 2175 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 2793 96
Size: 2697 2926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5623
BssSI C'ACGA_G
Cuts at: 2543 3683 4695 2543
Size: 1140 1012 3471
Fragments arranged by size:
3471 1140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5623
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5623
BstYI r'GATC_y
Cuts at: 1972 2308 2953 3320 3705 3722 5174 5260 5272
Size: 336 645 367 385 17 1452 86 12
Cuts at: 5272 5618 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 645 385 367 346 336 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2051 513
Size: 552 939 47 4085
Fragments arranged by size:
4085 939 552 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4884
Fragments arranged by size:
4884 683 56
BtsI GCAGTG_nn'
Cuts at: 2379 3139 3933 3953 2379
Size: 760 794 20 4049
Fragments arranged by size:
4049 794 760 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5588
DraI TTT'AAA
Cuts at: 2927 2927
Size: 5623
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2752 3070 3959
Size: 54 1425 50 275 18 922 318 889
Cuts at: 3959 5588 8
Size: 1629 43
Fragments arranged by size:
1629 1425 922 889 318 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3553 1353
Size: 2200 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4210 4584 4730 387
Size: 1064 138 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2472 2472
Size: 5623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2690 2858 2904 3686 4283 5070 5338
Size: 393 1058 168 46 782 597 787 268
Cuts at: 5338 1239
Size: 1524
Fragments arranged by size:
1524 1058 787 782 597 393 268 168
46
EcoNI CCTnn'n_nnAGG
Cuts at: 2234 2234
Size: 5623
EcoRI G'AATT_C
Cuts at: 2932 3310 2932
Size: 378 5245
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 2075 2099 4770 1678
Size: 397 24 2671 2531
Fragments arranged by size:
2671 2531 397 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4264 4296 4338 4370 1373
Size: 32 135 32 2692 32 42 32 2626
Fragments arranged by size:
2692 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2967 3213 669
Size: 988 281 1029 246 3079
Fragments arranged by size:
3079 1029 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5623
HpaI GTT'AAC
Cuts at: 3213 3213
Size: 5623
KasI G'GCGC_C
Cuts at: 2095 2095
Size: 5623
KpnI G_GTAC'C
Cuts at: 2948 2948
Size: 5623
MfeI C'AATT_G
Cuts at: 3222 3222
Size: 5623
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4737 4921 1305
Size: 3432 184 2007
Fragments arranged by size:
3432 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2754 10
Size: 54 1425 343 922 2879
Fragments arranged by size:
2879 1425 922 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 2481 3581 3940 4099 518
Size: 648 1270 45 1100 359 159 2042
Fragments arranged by size:
2042 1270 1100 648 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2101 2136 3039 3716 4864 5109 1606
Size: 495 35 903 677 1148 245 2120
Fragments arranged by size:
2120 1148 903 677 495 245 35
NaeI GCC'GGC
Cuts at: 2094 2094
Size: 5623
NarI GG'CG_CC
Cuts at: 2096 2096
Size: 5623
NcoI C'CATG_G
Cuts at: 513 1065 2004 2051 513
Size: 552 939 47 4085
Fragments arranged by size:
4085 939 552 47
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5623
NgoMIV G'CCGG_C
Cuts at: 2092 2092
Size: 5623
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2981 4526 1132
Size: 97 1752 1545 2229
Fragments arranged by size:
2229 1752 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2350 1348
Size: 1002 4621
PciI A'CATG_T
Cuts at: 4522 4522
Size: 5623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2918 1108
Size: 1810 3813
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 5205 5237 3692
Size: 32 1481 32 4078
Fragments arranged by size:
4078 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5623
PsiI TTA'TAA
Cuts at: 3193 3193
Size: 5623
PstI C_TGCA'G
Cuts at: 830 2079 2975 830
Size: 1249 896 3478
Fragments arranged by size:
3478 1249 896
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5623
PvuII CAG'CTG
Cuts at: 2136 2136
Size: 5623
SacI G_AGCT'C
Cuts at: 2474 2474
Size: 5623
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SbfI CC_TGCA'GG
Cuts at: 2975 2975
Size: 5623
ScaI AGT'ACT
Cuts at: 3871 5583 3871
Size: 1712 3911
SfcI C'TryA_G
Cuts at: 826 1045 2075 2718 2856 2971 4106 4787 4978
Size: 219 1030 643 138 115 1135 681 191
Cuts at: 4978 826
Size: 1471
Fragments arranged by size:
1471 1135 1030 681 643 219 191 138
115
SfoI GGC'GCC
Cuts at: 2097 2097
Size: 5623
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 2092 1700
Size: 392 5231
SmaI CCC'GGG
Cuts at: 1872 2950 1872
Size: 1078 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2149 3727 4628 4890 820
Size: 197 926 206 1578 901 262 1553
Fragments arranged by size:
1578 1553 926 901 262 206 197
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2981 1132
Size: 1849 3774
SspI AAT'ATT
Cuts at: 5 52 3547 5
Size: 47 3495 2081
Fragments arranged by size:
3495 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5623
StyI C'CwwG_G
Cuts at: 513 1065 2004 2051 2760 2917 3062 513
Size: 552 939 47 709 157 145 3074
Fragments arranged by size:
3074 939 709 552 157 145 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2147 3650 3667 3820 4005 4450 325
Size: 1255 567 1503 17 153 185 445 1498
Fragments arranged by size:
1503 1498 1255 567 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2793 3869 5581
Size: 274 80 33 51 1443 816 1076 1712
Cuts at: 5581 96
Size: 138
Fragments arranged by size:
1712 1443 1076 816 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 5583 5615 2829
Size: 32 2722 32 2837
Fragments arranged by size:
2837 2722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5623
XbaI T'CTAG_A
Cuts at: 2959 2959
Size: 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5623
XmaI C'CCGG_G
Cuts at: 1870 2948 1870
Size: 1078 4545
XmnI GAAnn'nnTTC
Cuts at: 2252 2769 3752 2252
Size: 517 983 4123
Fragments arranged by size:
4123 983 517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3431 276
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI