Restriction map for
pFN21AE4305
(Circular) (Six-base) MAPSORT of: pFN21AE4305.seq Check: 9535 from: 1 to: 5620
pFN21AE4305 5620 bp
With 182 enzymes: SgfI *
March 28, 2012 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2079 2774 2079
Size: 695 4925
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2907 2963 1937
Size: 970 56 4594
Fragments arranged by size:
4594 970 56
Acc65I G'GTAC_C
Cuts at: 2122 2597 2941 2122
Size: 475 344 4801
Fragments arranged by size:
4801 475 344
AclI AA'CG_TT
Cuts at: 1563 2650 3747 4120 1563
Size: 1087 1097 373 3063
Fragments arranged by size:
3063 1097 1087 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3683 5067 5335 1632
Size: 2051 1384 268 1917
Fragments arranged by size:
2051 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2155 820
Size: 197 1138 4285
Fragments arranged by size:
4285 1138 197
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2157 2915 1166
Size: 991 758 3871
Fragments arranged by size:
3871 991 758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 3363 4935 3363
Size: 1572 4048
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2525 2696 2929 3155 3307 5416 1493
Size: 1032 171 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 233 226 171 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2448 2945 1870
Size: 73 505 497 4545
Fragments arranged by size:
4545 505 497 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2597 2941 4396 618
Size: 325 217 627 335 475 344 1455 1842
Fragments arranged by size:
1842 1455 627 475 344 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 928
Size: 971 245 4404
Fragments arranged by size:
4404 971 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2696 3512 4722 2696
Size: 816 1210 3594
Fragments arranged by size:
3594 1210 816
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2777
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1996 2345 2595 2772 4280 1239
Size: 757 349 250 177 1508 2579
Fragments arranged by size:
2579 1508 757 349 250 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2842 3745 4610 4908 2047
Size: 795 903 865 298 2759
Fragments arranged by size:
2759 903 865 795 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2736 4283 882
Size: 617 1237 1547 2219
Fragments arranged by size:
2219 1547 1237 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5620
BseYI C'CCAG_C
Cuts at: 2264 2480 2726 4823 5319 2264
Size: 216 246 2097 496 2565
Fragments arranged by size:
2565 2097 496 246 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2336 3681 3766 4837 2336
Size: 1345 85 1071 3119
Fragments arranged by size:
3119 1345 1071 85
BsmI GAATG_Cn'
Cuts at: 2314 2366 3131 3224 2314
Size: 52 765 93 4710
Fragments arranged by size:
4710 765 93 52
BsmBI CGTCTCn'nnnn_
Cuts at: 2879 2879
Size: 5620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2336 2939 3681 3766 4837 1391
Size: 399 546 603 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 546 399 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2585 2774 2975 844
Size: 1235 506 189 201 3489
Fragments arranged by size:
3489 1235 506 201 189
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2113 4115 4289 65
Size: 1183 865 2002 174 1396
Fragments arranged by size:
2002 1396 1183 865 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2340 794
Size: 1546 4074
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2159 2198 2159
Size: 39 5581
BstYI r'GATC_y
Cuts at: 1972 2221 2950 3317 3702 3719 5171 5257 5269
Size: 249 729 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 729 385 367 346 249 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2841 2853 513
Size: 552 1776 12 3280
Fragments arranged by size:
3280 1776 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2221 487
Size: 683 56 995 3886
Fragments arranged by size:
3886 995 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2785 2924 2785
Size: 139 5481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2856 2889
Size: 54 1425 50 275 18 450 576 33
Cuts at: 2889 3067 3956 5585 8
Size: 178 889 1629 43
Fragments arranged by size:
1629 1425 889 576 450 275 178 54
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3550 1353
Size: 2197 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1996 2345 2595 2772 3683 4280 5067
Size: 393 364 349 250 177 911 597 787
Cuts at: 5067 5335 1239
Size: 268 1524
Fragments arranged by size:
1524 911 787 597 393 364 349 268
250 177
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2075
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2016 2016
Size: 5620
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2794 2826 4261 4293 4335
Size: 32 135 32 1222 32 1435 32 42
Cuts at: 4335 4367 1373
Size: 32 2626
Fragments arranged by size:
2626 1435 1222 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2126 2601 2945 2126
Size: 475 344 4801
Fragments arranged by size:
4801 475 344
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2279 2773 4734 4918 1305
Size: 974 494 1961 184 2007
Fragments arranged by size:
2007 1961 974 494 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2282 2858 10
Size: 54 1425 343 450 576 2772
Fragments arranged by size:
2772 1425 576 450 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2196 2915 3578 3937 4096 518
Size: 648 991 39 719 663 359 159 2042
Fragments arranged by size:
2042 991 719 663 648 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 2843 3036 3713 4861 5106 1606
Size: 1237 193 677 1148 245 2120
Fragments arranged by size:
2120 1237 1148 677 245 193
NcoI C'CATG_G
Cuts at: 513 1065 2853 513
Size: 552 1788 3280
Fragments arranged by size:
3280 1788 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PfoI T'CCnGG_A
Cuts at: 2012 2012
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2016 2016
Size: 5620
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PspXI vC'TCGA_Gb
Cuts at: 2448 2448
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2076 2470 2972 830
Size: 1246 394 502 3478
Fragments arranged by size:
3478 1246 502 394
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SacII CC_GC'GG
Cuts at: 2844 2844
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2072 2466 2904 2968 4103 4784 4975
Size: 219 1027 394 438 64 1135 681 191
Cuts at: 4975 826
Size: 1471
Fragments arranged by size:
1471 1135 1027 681 438 394 219 191
64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2062 2155 2448 2821 3724 4625
Size: 197 926 119 93 293 373 903 901
Cuts at: 4625 4887 820
Size: 262 1553
Fragments arranged by size:
1553 926 903 901 373 293 262 197
119 93
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2349 1517
Size: 832 4788
StyI C'CwwG_G
Cuts at: 513 1065 2506 2853 3059 513
Size: 552 1441 347 206 3074
Fragments arranged by size:
3074 1441 552 347 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3647 3664 3817 4002 4447 325
Size: 1255 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2696 2696
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 2448 1943
Size: 505 5115
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI