Restriction map for
pFN21AE4301
(Circular) (Six-base) MAPSORT of: pFN21AE4301.seq Check: 3884 from: 1 to: 5596
pFN21AE4301 5596 bp
With 182 enzymes: SgfI *
March 28, 2012 14:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2805 2939 1937
Size: 868 134 4594
Fragments arranged by size:
4594 868 134
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3659 5043 5311 1632
Size: 2027 1384 268 1917
Fragments arranged by size:
2027 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 2133 4325 2133
Size: 2192 3404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 2104 2599 3339 4911 2104
Size: 495 740 1572 2789
Fragments arranged by size:
2789 1572 740 495
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2189 2456 2905 3131 3283 5392 1493
Size: 696 267 449 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 449 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2831 2921 1870
Size: 73 888 90 4545
Fragments arranged by size:
4545 888 90 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2510 2552 2559 2630
Size: 325 217 627 463 260 42 7 71
Cuts at: 2630 2759 2917 4372 618
Size: 129 158 1455 1842
Fragments arranged by size:
1842 1455 627 463 325 260 217 158
129 71 42 7
BanII G_rGCy'C
Cuts at: 1391 2856 2915 1391
Size: 1465 59 4072
Fragments arranged by size:
4072 1465 59
BbeI G_GCGC'C
Cuts at: 2563 2763 2563
Size: 200 5396
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 928
Size: 971 506 4119
Fragments arranged by size:
4119 971 506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2393 2535 2651
Size: 107 122 71 631 605 721 142 116
Cuts at: 2651 2655 4207 136
Size: 4 1552 1525
Fragments arranged by size:
1552 1525 721 631 605 142 122 116
107 71 4
BglII A'GATC_T
Cuts at: 2685 5591 2685
Size: 2906 2690
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2513 2588 2633 3657 4813 1790
Size: 723 75 45 1024 1156 2573
Fragments arranged by size:
2573 1156 1024 723 75 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 4256 1239
Size: 1040 1977 2579
Fragments arranged by size:
2579 1977 1040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3721 4586 4884 3721
Size: 865 298 4433
Fragments arranged by size:
4433 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4259 882
Size: 617 2760 2219
Fragments arranged by size:
2760 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2560 2760 3403 3785
Size: 53 83 186 936 1027 200 643 382
Cuts at: 3785 4420 275
Size: 635 1451
Fragments arranged by size:
1451 1027 936 643 635 382 200 186
83 53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5596
BseYI C'CCAG_C
Cuts at: 2581 2701 2845 4799 5295 2581
Size: 120 144 1954 496 2882
Fragments arranged by size:
2882 1954 496 144 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 1163
Size: 573 648 4375
Fragments arranged by size:
4375 648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2715 3657 3742 4813 2715
Size: 942 85 1071 3498
Fragments arranged by size:
3498 1071 942 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2513 2588 2633 2715 2856 2915 3657
Size: 399 723 75 45 82 141 59 742
Cuts at: 3657 3742 4813 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 399 141 85 82
75 59 45
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2951 844
Size: 2107 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2452 3483 1394
Size: 429 629 1031 3507
Fragments arranged by size:
3507 1031 629 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstXI CCAn_nnnn'nTGG
Cuts at: 2377 2377
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2685 2926 3293 3678 3695 5147 5233 5245
Size: 713 241 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 241 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5044
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 2713 3112 3906 3926 2713
Size: 399 794 20 4383
Fragments arranged by size:
4383 794 399 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 3043 3932
Size: 54 1425 50 275 18 555 658 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 658 555 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3526 1353
Size: 2173 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2500 2881 4183 4557 4703 387
Size: 1064 138 911 381 1302 374 146 1280
Fragments arranged by size:
1302 1280 1064 911 381 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 3659 4256 5043 5311 1239
Size: 393 647 1380 597 787 268 1524
Fragments arranged by size:
1524 1380 787 647 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2030 2030
Size: 5596
EcoRI G'AATT_C
Cuts at: 2189 2905 3283 2189
Size: 716 378 4502
Fragments arranged by size:
4502 716 378
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 2563 2763 4743 1678
Size: 885 200 1980 2531
Fragments arranged by size:
2531 1980 885 200
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2119 2132 2151 4237
Size: 32 135 32 528 19 13 19 2086
Cuts at: 4237 4269 4311 4343 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2086 528 135 42 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2238 2940 3186 669
Size: 988 281 300 702 246 3079
Fragments arranged by size:
3079 988 702 300 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KasI G'GCGC_C
Cuts at: 2559 2759 2559
Size: 200 5396
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 10
Size: 54 1425 343 555 3219
Fragments arranged by size:
3219 1425 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2780 3554 3913 4072 518
Size: 648 1614 774 359 159 2042
Fragments arranged by size:
2042 1614 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2257 2770 3012 3689 4837 5082 1606
Size: 486 165 513 242 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 513 486 245 242 165
NarI GG'CG_CC
Cuts at: 2560 2760 2560
Size: 200 5396
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2599 1108
Size: 1491 4105
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2208 2505 2625 2742 2948 830
Size: 1378 297 120 117 206 3478
Fragments arranged by size:
3478 1378 297 206 120 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2257 2257
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 2819 3844 5556 2819
Size: 1025 1712 2859
Fragments arranged by size:
2859 1712 1025
SfcI C'TryA_G
Cuts at: 826 1045 2204 2501 2621 2738 2944 4079 4760
Size: 219 1159 297 120 117 206 1135 681
Cuts at: 4760 4951 826
Size: 191 1471
Fragments arranged by size:
1471 1159 1135 681 297 219 206 191
120 117
SfoI GGC'GCC
Cuts at: 2561 2761 2561
Size: 200 5396
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3700 4601 4863 820
Size: 197 926 1757 901 262 1553
Fragments arranged by size:
1757 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5596
StyI C'CwwG_G
Cuts at: 513 1065 3035 513
Size: 552 1970 3074
Fragments arranged by size:
3074 1970 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2837 3623 3640 3793 3978 4423 325
Size: 1255 1257 786 17 153 185 445 1498
Fragments arranged by size:
1498 1257 1255 786 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2817 3842 5554
Size: 274 80 33 51 1443 840 1025 1712
Cuts at: 5554 96
Size: 138
Fragments arranged by size:
1712 1443 1025 840 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2400 2432 5556 5588 2400
Size: 32 3124 32 2408
Fragments arranged by size:
3124 2408 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI