Restriction map for
pFN21AE4294
(Circular) (Six-base) MAPSORT of: pFN21AE4294.seq Check: 4754 from: 1 to: 10750
pFN21AE4294 10750 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5886 6896 7731 5886
Size: 1010 835 8905
Fragments arranged by size:
8905 1010 835
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6201 8560 278
Size: 53 83 186 936 4665 2359 2468
Fragments arranged by size:
4665 2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2808 8093 1937
Size: 871 5285 4594
Fragments arranged by size:
5285 4594 871
Acc65I G'GTAC_C
Cuts at: 5787 8071 5787
Size: 2284 8466
AclI AA'CG_TT
Cuts at: 1563 8877 9250 1563
Size: 7314 373 3063
Fragments arranged by size:
7314 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 3380 3734 3831 6185 7696 8813 10197
Size: 1640 108 354 97 2354 1511 1117 1384
Cuts at: 10197 10465 1632
Size: 268 1917
Fragments arranged by size:
2354 1917 1640 1511 1384 1117 354 268
108 97
AfeI AGC'GCT
Cuts at: 3596 5816 3596
Size: 2220 8530
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10553
AflIII A'CryG_T
Cuts at: 1203 7359 9649 10626 1203
Size: 6156 2290 977 1327
Fragments arranged by size:
6156 2290 1327 977
AgeI A'CCGG_T
Cuts at: 9581 9581
Size: 10750
AhdI GACnn_n'nnGTC
Cuts at: 4684 6166 9479 4684
Size: 1482 3313 5955
Fragments arranged by size:
5955 3313 1482
AleI CACnn'nnGTG
Cuts at: 1166 6653 6695 7913 1166
Size: 5487 42 1218 4003
Fragments arranged by size:
5487 4003 1218 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2723 1184
Size: 1539 9211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6461 6493 6461
Size: 32 10718
AlwNI CAG_nnn'CTG
Cuts at: 2275 4516 4612 4927 5656 7489 7723 8493 10065
Size: 2241 96 315 729 1833 234 770 1572
Cuts at: 10065 2275
Size: 2960
Fragments arranged by size:
2960 2241 1833 1572 770 729 315 234
96
ApaI G_GGCC'C
Cuts at: 3994 5524 5785 3994
Size: 1530 261 8959
Fragments arranged by size:
8959 1530 261
ApaLI G'TGCA_C
Cuts at: 7918 8807 9963 7918
Size: 889 1156 8705
Fragments arranged by size:
8705 1156 889
ApoI r'AATT_y
Cuts at: 1493 3626 5831 6919 8059 8285 8437 10546 1493
Size: 2133 2205 1088 1140 226 152 2109 1697
Fragments arranged by size:
2205 2133 2109 1697 1140 1088 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9304 160
Size: 872 8272 1606
Fragments arranged by size:
8272 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10750
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 6118 7854 8075 1870
Size: 73 271 3904 1736 221 4545
Fragments arranged by size:
4545 3904 1736 271 221 73
AvrII C'CTAG_G
Cuts at: 6268 6268
Size: 10750
BamHI G'GATC_C
Cuts at: 8080 8447 8080
Size: 367 10383
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2210 2496 4715 5025
Size: 325 217 627 315 108 286 2219 310
Cuts at: 5025 5765 5787 5874 6263 6983 7260 7423 7817
Size: 740 22 87 389 720 277 163 394
Cuts at: 7817 7947 7973 8071 9526 618
Size: 130 26 98 1455 1842
Fragments arranged by size:
2219 1842 1455 740 720 627 394 389
325 315 310 286 277 217 163 130
108 98 87 26 22
BanII G_rGCy'C
Cuts at: 1391 2358 2853 3732 3765 3994 4044 4823 5274
Size: 967 495 879 33 229 50 779 451
Cuts at: 5274 5524 5785 6012 7191 8069 1391
Size: 250 261 227 1179 878 4072
Fragments arranged by size:
4072 1179 967 879 878 779 495 451
261 250 229 227 50 33
BbeI G_GCGC'C
Cuts at: 2214 6267 7821 7977 2214
Size: 4053 1554 156 4987
Fragments arranged by size:
4987 4053 1554 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2631 3819 4866 5583 7062 928
Size: 971 732 1188 1047 717 1479 4616
Fragments arranged by size:
4616 1479 1188 1047 971 732 717
BbvCI CC'TCA_GC
Cuts at: 5870 5870
Size: 10750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2843 2877 4483 4517 8941 8975 1903
Size: 34 906 34 1606 34 4424 34 3678
Fragments arranged by size:
4424 3678 1606 906 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6862 8642 9852 6862
Size: 1780 1210 7760
Fragments arranged by size:
7760 1780 1210
BclI T'GATC_A
Cuts at: 1548 3927 4848 5112 5428 1548
Size: 2379 921 264 316 6870
Fragments arranged by size:
6870 2379 921 316 264
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2381 4121 4568
Size: 107 122 71 631 605 709 1740 447
Cuts at: 4568 5757 7898 9361 136
Size: 1189 2141 1463 1525
Fragments arranged by size:
2141 1740 1525 1463 1189 709 631 605
447 122 107 71
BglII A'GATC_T
Cuts at: 3972 7170 10745 3972
Size: 3198 3575 3977
Fragments arranged by size:
3977 3575 3198
BlpI GC'TnA_GC
Cuts at: 3888 6020 8167 3888
Size: 2132 2147 6471
Fragments arranged by size:
6471 2147 2132
Bme1580I G_kGCm'C
Cuts at: 1790 2499 3994 5496 5524 5785 6284 6842 6986
Size: 709 1495 1502 28 261 499 558 144
Cuts at: 6986 7324 7481 7922 8811 9967 1790
Size: 338 157 441 889 1156 2573
Fragments arranged by size:
2573 1502 1495 1156 889 709 558 499
441 338 261 157 144 28
BmgBI CAC'GTC
Cuts at: 1318 4468 4489 4603 5320 5413 7450 1318
Size: 3150 21 114 717 93 2037 4618
Fragments arranged by size:
4618 3150 2037 717 114 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5532 6407 7073
Size: 416 53 83 399 24 4115 875 666
Cuts at: 7073 8481 9439 442
Size: 1408 958 1753
Fragments arranged by size:
4115 1753 1408 958 875 666 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10750
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7453 7453
Size: 10750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2324 3223 3502 3989 4028 4196 4351
Size: 1007 78 899 279 487 39 168 155
Cuts at: 4351 4649 5723 5738 6026 6398 6752 6858 7187
Size: 298 1074 15 288 372 354 106 329
Cuts at: 7187 7205 7823 7981 8045 9410 1239
Size: 18 618 158 64 1365 2579
Fragments arranged by size:
2579 1365 1074 1007 899 618 487 372
354 329 298 288 279 168 158 155
106 78 64 39 18 15
Bpu10I CC'TnA_GC
Cuts at: 3771 4547 5870 3771
Size: 776 1323 8651
Fragments arranged by size:
8651 1323 776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2444 3457 3459 4045 6812 7558 8875 9740 10038
Size: 1013 2 586 2767 746 1317 865 298
Cuts at: 10038 2444
Size: 3156
Fragments arranged by size:
3156 2767 1317 1013 865 746 586 298
2
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2193 7700 7746 9413 882
Size: 617 694 5507 46 1667 2219
Fragments arranged by size:
5507 2219 1667 694 617 46
BsaAI yAC'GTr
Cuts at: 493 1204 4165 4473 5491 8541 493
Size: 711 2961 308 1018 3050 2702
Fragments arranged by size:
3050 2961 2702 1018 711 308
BsaBI GATnn'nnATC
Cuts at: 3929 5111 7490 10744 3929
Size: 1182 2379 3254 3935
Fragments arranged by size:
3935 3254 2379 1182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 4649 5471 6198
Size: 53 83 186 936 678 2438 822 727
Cuts at: 6198 6264 7818 7974 8557 8939 9574 275
Size: 66 1554 156 583 382 635 1451
Fragments arranged by size:
2438 1554 1451 936 822 727 678 635
583 382 186 156 83 66 53
BsaWI w'CCGG_w
Cuts at: 2916 9183 9581 9855 10002 10413 2916
Size: 6267 398 274 147 411 3253
Fragments arranged by size:
6267 3253 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3679 3709 5597 5627 6986
Size: 30 165 30 2276 30 1888 30 1359
Cuts at: 6986 7016 7145 7175 7505 7535 1178
Size: 30 129 30 330 30 4393
Fragments arranged by size:
4393 2276 1888 1359 330 165 129 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2243 4208 6689 7262 7484 7528 8024
Size: 886 171 1965 2481 573 222 44 496
Cuts at: 8024 1186
Size: 3912
Fragments arranged by size:
3912 2481 1965 886 573 496 222 171
44
BseYI C'CCAG_C
Cuts at: 2030 2253 2371 2451 2696 2744 3775 3784 4186
Size: 223 118 80 245 48 1031 9 402
Cuts at: 4186 5523 6510 7343 7474 7583 7591 7821 7968
Size: 1337 987 833 131 109 8 230 147
Cuts at: 7968 8014 9953 10449 2030
Size: 46 1939 496 2331
Fragments arranged by size:
2331 1939 1337 1031 987 833 496 402
245 230 223 147 131 118 109 80
48 46 9 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2984 4495 4625 5579 5921 6119
Size: 573 696 552 1511 130 954 342 198
Cuts at: 6119 6378 6494 6746 7246 1163
Size: 259 116 252 500 4667
Fragments arranged by size:
4667 1511 954 696 573 552 500 342
259 252 198 130 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6536 8200 8961 9989 1540
Size: 275 186 4535 1664 761 1028 2301
Fragments arranged by size:
4535 2301 1664 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2853 3732 3765 3889 4044 5724 6012 6210 6366
Size: 879 33 124 155 1680 288 198 156
Cuts at: 6366 7016 7922 8811 8896 9967 2853
Size: 650 906 889 85 1071 3636
Fragments arranged by size:
3636 1680 1071 906 889 879 650 288
198 156 155 124 85 33
BsmI GAATG_Cn'
Cuts at: 8261 8354 8261
Size: 93 10657
BsmBI CGTCTCn'nnnn_
Cuts at: 2084 2084
Size: 10750
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2358 2499 2853 3732 3765 3889 3994
Size: 399 568 141 354 879 33 124 105
Cuts at: 3994 4044 4823 5274 5496 5524 5724 5785 6012
Size: 50 779 451 222 28 200 61 227
Cuts at: 6012 6210 6284 6366 6842 6986 7016 7191 7324
Size: 198 74 82 476 144 30 175 133
Cuts at: 7324 7481 7922 8069 8811 8896 9967 1391
Size: 157 441 147 742 85 1071 2174
Fragments arranged by size:
2174 1071 879 779 742 568 476 451
441 399 354 227 222 200 198 175
157 147 144 141 133 124 105 85
82 74 61 50 33 30 28
BspEI T'CCGG_A
Cuts at: 2916 10413 2916
Size: 7497 3253
BspHI T'CATG_A
Cuts at: 5673 6498 8639 10369 5673
Size: 825 2141 1730 6054
Fragments arranged by size:
6054 2141 1730 825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 3497 3888 4689 5142 5886 6141 6896
Size: 1385 1268 391 801 453 744 255 755
Cuts at: 6896 7731 8105 844
Size: 835 374 3489
Fragments arranged by size:
3489 1385 1268 835 801 755 744 453
391 374 255
BsrBI CCG'CTC
Cuts at: 1394 1823 4353 5602 5992 6019 8637 1394
Size: 429 2530 1249 390 27 2618 3507
Fragments arranged by size:
3507 2618 2530 1249 429 390 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 2628 3835 3873 7135 7836 9245 9419
Size: 1183 1380 1207 38 3262 701 1409 174
Cuts at: 9419 65
Size: 1396
Fragments arranged by size:
3262 1409 1396 1380 1207 1183 701 174
38
BsrFI r'CCGG_y
Cuts at: 1700 1810 4051 4111 4712 5854 8019 9394 9581
Size: 110 2241 60 601 1142 2165 1375 187
Cuts at: 9581 1700
Size: 2869
Fragments arranged by size:
2869 2241 2165 1375 1142 601 187 110
60
BsrGI T'GTAC_A
Cuts at: 96 7461 96
Size: 7365 3385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10750
BssSI C'ACGA_G
Cuts at: 8810 9822 8810
Size: 1012 9738
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2419 5491 5944 7876 794
Size: 1625 3072 453 1932 3668
Fragments arranged by size:
3668 3072 1932 1625 453
BstBI TT'CG_AA
Cuts at: 3108 9554 3108
Size: 6446 4304
BstEII G'GTnAC_C
Cuts at: 1184 2141 1184
Size: 957 9793
BstXI CCAn_nnnn'nTGG
Cuts at: 2872 3270 3967 7096 7285 2872
Size: 398 697 3129 189 6337
Fragments arranged by size:
6337 3129 697 398 189
BstYI r'GATC_y
Cuts at: 1972 2299 3265 3511 3972 4803 6779 7170 7485
Size: 327 966 246 461 831 1976 391 315
Cuts at: 7485 7737 8080 8447 8832 8849 10301 10387 10399
Size: 252 343 367 385 17 1452 86 12
Cuts at: 10399 10745 1972
Size: 346 1977
Fragments arranged by size:
1977 1976 1452 966 831 461 391 385
367 346 343 327 315 252 246 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4751 4865 7352 7428 4751
Size: 114 2487 76 8073
Fragments arranged by size:
8073 2487 114 76
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 2203 2358 2376 4080 4200
Size: 552 939 81 118 155 18 1704 120
Cuts at: 4200 4254 4744 5014 5391 6651 6972 7089 7206
Size: 54 490 270 377 1260 321 117 117
Cuts at: 7206 7695 7893 513
Size: 489 198 3370
Fragments arranged by size:
3370 1704 1260 939 552 490 489 377
321 270 198 155 120 118 117 117
81 54 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7030 7426 487
Size: 683 56 5804 396 3811
Fragments arranged by size:
5804 3811 683 396 56
BtsI GCAGTG_nn'
Cuts at: 5767 8266 9060 9080 5767
Size: 2499 794 20 7437
Fragments arranged by size:
7437 2499 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10750
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3464 3499 4236 4271 529
Size: 35 2900 35 737 35 7008
Fragments arranged by size:
7008 2900 737 35 35 35
DraI TTT'AAA
Cuts at: 8054 8054
Size: 10750
DraIII CAC_nnn'GTG
Cuts at: 4035 7944 4035
Size: 3909 6841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9757 809
Size: 507 8441 1802
Fragments arranged by size:
8441 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2604 4113 4122
Size: 54 1425 50 275 18 774 1509 9
Cuts at: 4122 4203 4833 4929 5592 5688 5805 6897 7410
Size: 81 630 96 663 96 117 1092 513
Cuts at: 7410 7953 8197 9086 10715 8
Size: 543 244 889 1629 43
Fragments arranged by size:
1629 1509 1425 1092 889 774 663 630
543 513 275 244 117 96 96 81
54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 8197 1537
Size: 275 6385 4090
Fragments arranged by size:
6385 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3710 4902 5702 8680 1353
Size: 2357 1192 800 2978 3423
Fragments arranged by size:
3423 2978 2357 1192 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5138 6434 9337 9711 9857 387
Size: 1064 138 3549 1296 2903 374 146 1280
Fragments arranged by size:
3549 2903 1296 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2851 3730 3763 4042 6010 2851
Size: 879 33 279 1968 7591
Fragments arranged by size:
7591 1968 879 279 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2324 3223 3272 3380 3502 3734
Size: 393 614 78 899 49 108 122 232
Cuts at: 3734 3831 3989 4028 4196 4351 4649 5723 5738
Size: 97 158 39 168 155 298 1074 15
Cuts at: 5738 6026 6185 6398 6752 6858 7187 7205 7696
Size: 288 159 213 354 106 329 18 491
Cuts at: 7696 7823 7981 8045 8813 9410 10197 10465 1239
Size: 127 158 64 768 597 787 268 1524
Fragments arranged by size:
1524 1074 899 787 768 614 597 491
393 354 329 298 288 268 232 213
168 159 158 158 155 127 122 108
106 97 78 64 49 39 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 4556 5750 6719 7669 7805 2225
Size: 2331 1194 969 950 136 5170
Fragments arranged by size:
5170 2331 1194 969 950 136
EcoO109I rG'GnC_Cy
Cuts at: 2218 2438 2655 2902 3857 3938 3990 3991 4156
Size: 220 217 247 955 81 52 1 165
Cuts at: 4156 4243 4678 6298 6568 6712 7339 7566 7765
Size: 87 435 1620 270 144 627 227 199
Cuts at: 7765 2218
Size: 5203
Fragments arranged by size:
5203 1620 955 627 435 270 247 227
220 217 199 165 144 87 81 52
1
EcoRI G'AATT_C
Cuts at: 6919 8059 8437 6919
Size: 1140 378 9232
Fragments arranged by size:
9232 1140 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3666 5083 3666
Size: 1417 9333
FspI TGC'GCA
Cuts at: 9256 9256
Size: 10750
HaeII r_GCGC'y
Cuts at: 1678 2214 3598 4187 4585 5818 5969 6267 6391
Size: 536 1384 589 398 1233 151 298 124
Cuts at: 6391 6497 7821 7977 9897 1678
Size: 106 1324 156 1920 2531
Fragments arranged by size:
2531 1920 1384 1324 1233 589 536 398
298 156 151 124 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4670 4702 5597 5629 6072
Size: 32 135 32 3098 32 895 32 443
Cuts at: 6072 6104 6152 6184 6298 6330 6332 6362 6364
Size: 32 48 32 114 32 2 30 2
Cuts at: 6364 6394 6986 7018 7145 7177 7505 7537 7773
Size: 30 592 32 127 32 328 32 236
Cuts at: 7773 7805 9391 9423 9465 9497 1373
Size: 32 1586 32 42 32 2626
Fragments arranged by size:
3098 2626 1586 895 592 443 328 236
135 127 114 48 42 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4132 7102 8094 8340 669
Size: 988 281 2194 2970 992 246 3079
Fragments arranged by size:
3079 2970 2194 992 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3297 748
Size: 2549 8201
HpaI GTT'AAC
Cuts at: 8340 8340
Size: 10750
KasI G'GCGC_C
Cuts at: 2210 6263 7817 7973 2210
Size: 4053 1554 156 4987
Fragments arranged by size:
4987 4053 1554 156
KpnI G_GTAC'C
Cuts at: 5791 8075 5791
Size: 2284 8466
MfeI C'AATT_G
Cuts at: 8349 8349
Size: 10750
MluI A'CGCG_T
Cuts at: 10626 10626
Size: 10750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2958 5340 6150 7692 9864 10048 1305
Size: 1653 2382 810 1542 2172 184 2007
Fragments arranged by size:
2382 2172 2007 1653 1542 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4124 4205 4835 4931 10
Size: 54 1425 343 2292 81 630 96 5829
Fragments arranged by size:
5829 2292 1425 630 343 96 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4407 6182 6587 6653 6695 6722 7094
Size: 648 3241 1775 405 66 42 27 372
Cuts at: 7094 7913 8708 9067 9226 518
Size: 819 795 359 159 2042
Fragments arranged by size:
3241 2042 1775 819 795 648 405 372
359 159 66 42 27
MspA1I CmG'CkG
Cuts at: 1606 2066 2205 2257 2455 3457 3784 3802 3892
Size: 460 139 52 198 1002 327 18 90
Cuts at: 3892 4456 4627 4636 5647 6538 6878 6999 7107
Size: 564 171 9 1011 891 340 121 108
Cuts at: 7107 7315 7825 8166 8843 9991 10236 1606
Size: 208 510 341 677 1148 245 2120
Fragments arranged by size:
2120 1148 1011 1002 891 677 564 510
460 341 340 327 245 208 198 171
139 121 108 90 52 18 9
NaeI GCC'GGC
Cuts at: 4053 8021 4053
Size: 3968 6782
NarI GG'CG_CC
Cuts at: 2211 6264 7818 7974 2211
Size: 4053 1554 156 4987
Fragments arranged by size:
4987 4053 1554 156
NcoI C'CATG_G
Cuts at: 513 1065 2004 2085 5391 7089 7206 7695 513
Size: 552 939 81 3306 1698 117 489 3568
Fragments arranged by size:
3568 3306 1698 939 552 489 117 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10750
NgoMIV G'CCGG_C
Cuts at: 4051 8019 4051
Size: 3968 6782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10750
NotI GC'GGCC_GC
Cuts at: 8197 8197
Size: 10750
NspI r_CATG'y
Cuts at: 1132 1229 2017 2038 5935 6088 6838 7363 7645
Size: 97 788 21 3897 153 750 525 282
Cuts at: 7645 8108 9653 1132
Size: 463 1545 2229
Fragments arranged by size:
3897 2229 1545 788 750 525 463 282
153 97 21
PasI CC'CwG_GG
Cuts at: 1348 2802 2898 3270 3403 6076 6634 6743 1348
Size: 1454 96 372 133 2673 558 109 5355
Fragments arranged by size:
5355 2673 1454 558 372 133 109 96
PciI A'CATG_T
Cuts at: 7359 9649 7359
Size: 2290 8460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3129 4035 4516 4927 5259 5803 1108
Size: 2021 906 481 411 332 544 6055
Fragments arranged by size:
6055 2021 906 544 481 411 332
PfoI T'CCnGG_A
Cuts at: 2755 3040 3599 3756 3966 4731 5700 6680 2755
Size: 285 559 157 210 765 969 980 6825
Fragments arranged by size:
6825 980 969 765 559 285 210 157
PmeI GTTT'AAAC
Cuts at: 8054 8054
Size: 10750
PmlI CAC'GTG
Cuts at: 4473 4473
Size: 10750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8819 8851 10332 10364 8819
Size: 32 1481 32 9205
Fragments arranged by size:
9205 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 2438 3938 4678 6568 7339 7566 7765 2218
Size: 220 1500 740 1890 771 227 199 5203
Fragments arranged by size:
5203 1890 1500 771 740 227 220 199
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10750
PsiI TTA'TAA
Cuts at: 8320 8320
Size: 10750
PspOMI G'GGCC_C
Cuts at: 3990 5520 5781 3990
Size: 1530 261 8959
Fragments arranged by size:
8959 1530 261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4907 4939 4907
Size: 32 10718
PstI C_TGCA'G
Cuts at: 830 2152 2447 3494 3841 4557 4686 4800 5139
Size: 1322 295 1047 347 716 129 114 339
Cuts at: 5139 5652 5949 6492 7178 7728 8102 830
Size: 513 297 543 686 550 374 3478
Fragments arranged by size:
3478 1322 1047 716 686 550 543 513
374 347 339 297 295 129 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10750
PvuII CAG'CTG
Cuts at: 2066 2257 3457 3784 3802 3892 4456 4627 4636
Size: 191 1200 327 18 90 564 171 9
Cuts at: 4636 6878 6999 7315 2066
Size: 2242 121 316 5501
Fragments arranged by size:
5501 2242 1200 564 327 316 191 171
121 90 18 9
SacI G_AGCT'C
Cuts at: 2853 3732 3765 4044 6012 2853
Size: 879 33 279 1968 7591
Fragments arranged by size:
7591 1968 879 279 33
SacII CC_GC'GG
Cuts at: 2206 2206
Size: 10750
SalI G'TCGA_C
Cuts at: 1936 8092 1936
Size: 6156 4594
SanDI GG'GwC_CC
Cuts at: 3938 7339 7765 3938
Size: 3401 426 6923
Fragments arranged by size:
6923 3401 426
SapI GCTCTTCn'nnn_
Cuts at: 3710 4902 5702 3710
Size: 1192 800 8758
Fragments arranged by size:
8758 1192 800
SbfI CC_TGCA'GG
Cuts at: 3494 4557 7728 8102 3494
Size: 1063 3171 374 6142
Fragments arranged by size:
6142 3171 1063 374
ScaI AGT'ACT
Cuts at: 2720 4916 5564 8998 10710 2720
Size: 2196 648 3434 1712 2760
Fragments arranged by size:
3434 2760 2196 1712 648
SexAI A'CCwGG_T
Cuts at: 2434 6927 2434
Size: 4493 6257
SfcI C'TryA_G
Cuts at: 826 1045 2148 2443 3149 3490 3837 4553 4682
Size: 219 1103 295 706 341 347 716 129
Cuts at: 4682 4796 5135 5221 5648 5945 6488 7174 7724
Size: 114 339 86 427 297 543 686 550
Cuts at: 7724 8098 9233 9914 10105 826
Size: 374 1135 681 191 1471
Fragments arranged by size:
1471 1135 1103 716 706 686 681 550
543 427 374 347 341 339 297 295
219 191 129 114 86
SfiI GGCCn_nnn'nGGCC
Cuts at: 4121 4121
Size: 10750
SfoI GGC'GCC
Cuts at: 2212 6265 7819 7975 2212
Size: 4053 1554 156 4987
Fragments arranged by size:
4987 4053 1554 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10750
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10750
SmaI CCC'GGG
Cuts at: 1872 2216 8077 1872
Size: 344 5861 4545
Fragments arranged by size:
5861 4545 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 3436 3474 4060 6827 7573
Size: 197 926 480 1013 38 586 2767 746
Cuts at: 7573 8854 9755 10017 820
Size: 1281 901 262 1553
Fragments arranged by size:
2767 1553 1281 1013 926 901 746 586
480 262 197 38
SnaBI TAC'GTA
Cuts at: 493 8541 493
Size: 8048 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10750
SphI G_CATG'C
Cuts at: 1132 5935 8108 1132
Size: 4803 2173 3774
Fragments arranged by size:
4803 3774 2173
SspI AAT'ATT
Cuts at: 5 52 2637 4813 8674 5
Size: 47 2585 2176 3861 2081
Fragments arranged by size:
3861 2585 2176 2081 47
StuI AGG'CCT
Cuts at: 1517 2234 6313 6863 1517
Size: 717 4079 550 5404
Fragments arranged by size:
5404 4079 717 550
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2478 4068 4239 5391 6268
Size: 552 939 81 393 1590 171 1152 877
Cuts at: 6268 6435 6864 7089 7206 7284 7377 7695 8189
Size: 167 429 225 117 78 93 318 494
Cuts at: 8189 513
Size: 3074
Fragments arranged by size:
3074 1590 1152 939 877 552 494 429
393 318 225 171 167 117 93 81
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4815 5027 5693 6107 8777 8794 8947
Size: 1255 3235 212 666 414 2670 17 153
Cuts at: 8947 9132 9577 325
Size: 185 445 1498
Fragments arranged by size:
3235 2670 1498 1255 666 445 414 212
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2037 2718 4914
Size: 274 80 33 51 1443 60 681 2196
Cuts at: 4914 5562 6645 7461 8996 10708 96
Size: 648 1083 816 1535 1712 138
Fragments arranged by size:
2196 1712 1535 1443 1083 816 681 648
274 138 80 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3679 3711 5402 5434 10710 10742 3679
Size: 32 1691 32 5276 32 3687
Fragments arranged by size:
5276 3687 1691 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3689 5411 5582 1316
Size: 2373 1722 171 6484
Fragments arranged by size:
6484 2373 1722 171
XbaI T'CTAG_A
Cuts at: 8086 8086
Size: 10750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3038 3310 4004 4052 4303 5698 5759 3038
Size: 272 694 48 251 1395 61 8029
Fragments arranged by size:
8029 1395 694 272 251 61 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10750
XmaI C'CCGG_G
Cuts at: 1870 2214 8075 1870
Size: 344 5861 4545
Fragments arranged by size:
5861 4545 344
XmnI GAAnn'nnTTC
Cuts at: 2570 5903 8879 2570
Size: 3333 2976 4441
Fragments arranged by size:
4441 3333 2976
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6199 8558 276
Size: 53 83 186 936 4665 2359 2468
Fragments arranged by size:
4665 2468 2359 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI
NruI NsiI PacI PspXI RsrII SrfI SwaI