Restriction map for
pFN21AE4288
(Circular) (Six-base) MAPSORT of: pFN21AE4288.seq Check: 2187 from: 1 to: 9754
pFN21AE4288 9754 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7564 278
Size: 53 83 186 936 6028 2468
Fragments arranged by size:
6028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7097 1937
Size: 5160 4594
Acc65I G'GTAC_C
Cuts at: 7075 7075
Size: 9754
AclI AA'CG_TT
Cuts at: 1563 7881 8254 1563
Size: 6318 373 3063
Fragments arranged by size:
6318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2530 4058 4112 4895 5370 5555 6197
Size: 719 179 1528 54 783 475 185 642
Cuts at: 6197 6824 7062 7817 9201 9469 1632
Size: 627 238 755 1384 268 1917
Fragments arranged by size:
1917 1528 1384 783 755 719 642 627
475 268 238 185 179 54
AfeI AGC'GCT
Cuts at: 4403 4403
Size: 9754
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9557
AflIII A'CryG_T
Cuts at: 1203 3329 3331 3857 8653 9630 1203
Size: 2126 2 526 4796 977 1327
Fragments arranged by size:
4796 2126 1327 977 526 2
AgeI A'CCGG_T
Cuts at: 8585 8585
Size: 9754
AhdI GACnn_n'nnGTC
Cuts at: 3185 6348 8483 3185
Size: 3163 2135 4456
Fragments arranged by size:
4456 3163 2135
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9754
AlwNI CAG_nnn'CTG
Cuts at: 2452 3647 7497 9069 2452
Size: 1195 3850 1572 3137
Fragments arranged by size:
3850 3137 1572 1195
ApaLI G'TGCA_C
Cuts at: 6857 7811 8967 6857
Size: 954 1156 7644
Fragments arranged by size:
7644 1156 954
ApoI r'AATT_y
Cuts at: 1493 2398 2643 2695 3085 3111 3293 3603 3869
Size: 905 245 52 390 26 182 310 266
Cuts at: 3869 5456 5951 6665 7063 7289 7441 9550 1493
Size: 1587 495 714 398 226 152 2109 1697
Fragments arranged by size:
2109 1697 1587 905 714 495 398 390
310 266 245 226 182 152 52 26
AseI AT'TA_AT
Cuts at: 160 1032 4527 8308 160
Size: 872 3495 3781 1606
Fragments arranged by size:
3781 3495 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9754
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 4417 5868 7079 1870
Size: 73 1269 1205 1451 1211 4545
Fragments arranged by size:
4545 1451 1269 1211 1205 73
AvrII C'CTAG_G
Cuts at: 6104 6104
Size: 9754
BamHI G'GATC_C
Cuts at: 7084 7451 7084
Size: 367 9387
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5250 7075 8530 618
Size: 325 217 627 3463 1825 1455 1842
Fragments arranged by size:
3463 1842 1825 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4424 4873 6816 7073 1391
Size: 3033 449 1943 257 4072
Fragments arranged by size:
4072 3033 1943 449 257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5561 928
Size: 971 3662 5121
Fragments arranged by size:
5121 3662 971
BbvCI CC'TCA_GC
Cuts at: 4671 4928 4671
Size: 257 9497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7945 7979 1903
Size: 34 6008 34 3678
Fragments arranged by size:
6008 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4929 5253 7646 8856 4929
Size: 324 2393 1210 5827
Fragments arranged by size:
5827 2393 1210 324
BclI T'GATC_A
Cuts at: 1548 4003 4161 1548
Size: 2455 158 7141
Fragments arranged by size:
7141 2455 158
BfrBI ATG'CAT
Cuts at: 2080 6372 2080
Size: 4292 5462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8365 136
Size: 107 122 71 631 605 6693 1525
Fragments arranged by size:
6693 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9749 9749
Size: 9754
BlpI GC'TnA_GC
Cuts at: 4880 5092 7171 4880
Size: 212 2079 7463
Fragments arranged by size:
7463 2079 212
Bme1580I G_kGCm'C
Cuts at: 1790 2995 6861 7815 8971 1790
Size: 1205 3866 954 1156 2573
Fragments arranged by size:
3866 2573 1205 1156 954
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9754
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4876 7485 8443
Size: 416 53 83 399 24 3459 2609 958
Cuts at: 8443 442
Size: 1753
Fragments arranged by size:
3459 2609 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2016 4536 4770 5666 8414 1239
Size: 777 2520 234 896 2748 2579
Fragments arranged by size:
2748 2579 2520 896 777 234
Bpu10I CC'TnA_GC
Cuts at: 4671 4928 4671
Size: 257 9497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2704 5309 6246 7879 8744 9042 2704
Size: 2605 937 1633 865 298 3416
Fragments arranged by size:
3416 2605 1633 937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2911 3491 5879 8417 882
Size: 617 1412 580 2388 2538 2219
Fragments arranged by size:
2538 2388 2219 1412 617 580
BsaAI yAC'GTr
Cuts at: 493 1204 3332 7545 493
Size: 711 2128 4213 2702
Fragments arranged by size:
4213 2702 2128 711
BsaBI GATnn'nnATC
Cuts at: 3684 9748 3684
Size: 6064 3690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4381 7561 7943 8578
Size: 53 83 186 936 2848 3180 382 635
Cuts at: 8578 275
Size: 1451
Fragments arranged by size:
3180 2848 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2794 8187 8585 8859 9006 9417 2794
Size: 5393 398 274 147 411 3131
Fragments arranged by size:
5393 3131 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4758 4788 6401 6431 1178
Size: 30 165 30 3355 30 1613 30 4501
Fragments arranged by size:
4501 3355 1613 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2019 5152 5486 5828 6515 1186
Size: 833 3133 334 342 687 4425
Fragments arranged by size:
4425 3133 833 687 342 334
BseYI C'CCAG_C
Cuts at: 4509 5355 8957 9453 4509
Size: 846 3602 496 4810
Fragments arranged by size:
4810 3602 846 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2364 2981 5396 6740 1163
Size: 573 628 617 2415 1344 4177
Fragments arranged by size:
4177 2415 1344 628 617 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7204 7965 8993 1540
Size: 275 186 5203 761 1028 2301
Fragments arranged by size:
5203 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2029 5083 5319 5857 6313 6861 7815 7900 8971
Size: 3054 236 538 456 548 954 85 1071
Cuts at: 8971 2029
Size: 2812
Fragments arranged by size:
3054 2812 1071 954 548 538 456 236
85
BsmI GAATG_Cn'
Cuts at: 5100 6374 6933 6982 7265 7358 5100
Size: 1274 559 49 283 93 7496
Fragments arranged by size:
7496 1274 559 283 93 49
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2995 4424 4873 5083 5319 5857
Size: 399 239 966 1429 449 210 236 538
Cuts at: 5857 6313 6816 6861 7073 7815 7900 8971 1391
Size: 456 503 45 212 742 85 1071 2174
Fragments arranged by size:
2174 1429 1071 966 742 538 503 456
449 399 239 236 212 210 85 45
BspEI T'CCGG_A
Cuts at: 2794 9417 2794
Size: 6623 3131
BspHI T'CATG_A
Cuts at: 2724 6019 7643 9373 2724
Size: 3295 1624 1730 3105
Fragments arranged by size:
3295 3105 1730 1624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7109 844
Size: 6265 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 7641 1394
Size: 429 5818 3507
Fragments arranged by size:
5818 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4137 4922 6078 6942 8249 8423 65
Size: 1183 2889 785 1156 864 1307 174 1396
Fragments arranged by size:
2889 1396 1307 1183 1156 864 785 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8398 8585 1700
Size: 110 6588 187 2869
Fragments arranged by size:
6588 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5142 6889 96
Size: 5046 1747 2961
Fragments arranged by size:
5046 2961 1747
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9754
BssSI C'ACGA_G
Cuts at: 3488 4023 7814 8826 3488
Size: 535 3791 1012 4416
Fragments arranged by size:
4416 3791 1012 535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6937 794
Size: 6143 3611
BstBI TT'CG_AA
Cuts at: 8558 8558
Size: 9754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9754
BstXI CCAn_nnnn'nTGG
Cuts at: 2881 5005 2881
Size: 2124 7630
BstYI r'GATC_y
Cuts at: 1972 4449 4573 5980 6404 6580 7084 7451 7836
Size: 2477 124 1407 424 176 504 367 385
Cuts at: 7836 7853 9305 9391 9403 9749 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2477 1977 1452 1407 504 424 385 367
346 176 124 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3508 513
Size: 552 2443 6759
Fragments arranged by size:
6759 2443 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9015
Fragments arranged by size:
9015 683 56
BtsI GCAGTG_nn'
Cuts at: 4117 7270 8064 8084 4117
Size: 3153 794 20 5787
Fragments arranged by size:
5787 3153 794 20
ClaI AT'CG_AT
Cuts at: 1554 4531 1554
Size: 2977 6777
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5253 5288 529
Size: 35 4689 35 4995
Fragments arranged by size:
4995 4689 35 35
DraI TTT'AAA
Cuts at: 6718 7058 6718
Size: 340 9414
DraIII CAC_nnn'GTG
Cuts at: 2842 2842
Size: 9754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8761 809
Size: 507 7445 1802
Fragments arranged by size:
7445 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5281 7201 8090
Size: 54 1425 50 275 18 3451 1920 889
Cuts at: 8090 9719 8
Size: 1629 43
Fragments arranged by size:
3451 1920 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7201 1537
Size: 275 5389 4090
Fragments arranged by size:
5389 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2158 2210 2256 2370 2582 2772 3213 3546
Size: 805 52 46 114 212 190 441 333
Cuts at: 3546 4236 5004 5289 5592 7038 7684 1353
Size: 690 768 285 303 1446 646 3423
Fragments arranged by size:
3423 1446 805 768 690 646 441 333
303 285 212 190 114 52 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6114 8341 8715 8861 387
Size: 1064 138 4525 2227 374 146 1280
Fragments arranged by size:
4525 2227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2016 2351 2530 4058 4112 4536 4770
Size: 393 384 335 179 1528 54 424 234
Cuts at: 4770 4895 5370 5555 5666 6197 6824 7062 7817
Size: 125 475 185 111 531 627 238 755
Cuts at: 7817 8414 9201 9469 1239
Size: 597 787 268 1524
Fragments arranged by size:
1528 1524 787 755 627 597 531 475
424 393 384 335 268 238 234 185
179 125 111 54
EcoO109I rG'GnC_Cy
Cuts at: 5651 5651
Size: 9754
EcoRI G'AATT_C
Cuts at: 3869 7063 7441 3869
Size: 3194 378 6182
Fragments arranged by size:
6182 3194 378
EcoRV GAT'ATC
Cuts at: 4354 4354
Size: 9754
FspI TGC'GCA
Cuts at: 8260 8260
Size: 9754
HaeII r_GCGC'y
Cuts at: 1678 4405 8901 1678
Size: 2727 4496 2531
Fragments arranged by size:
4496 2727 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2922 2954 3160 3171 3192
Size: 32 135 32 1350 32 206 11 21
Cuts at: 3192 3203 3369 3401 4153 4185 4524 4556 5700
Size: 11 166 32 752 32 339 32 1144
Cuts at: 5700 5732 5750 5782 6257 6289 6334 6366 6436
Size: 32 18 32 475 32 45 32 70
Cuts at: 6436 6468 6765 6797 8395 8427 8469 8501 1373
Size: 32 297 32 1598 32 42 32 2626
Fragments arranged by size:
2626 1598 1350 1144 752 475 339 297
206 166 135 70 45 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 21 18 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3773 4146 7098 7344 669
Size: 988 281 1835 373 2952 246 3079
Fragments arranged by size:
3079 2952 1835 988 373 281 246
HindIII A'AGCT_T
Cuts at: 748 3740 5361 5531 748
Size: 2992 1621 170 4971
Fragments arranged by size:
4971 2992 1621 170
HpaI GTT'AAC
Cuts at: 7344 7344
Size: 9754
KpnI G_GTAC'C
Cuts at: 7079 7079
Size: 9754
MfeI C'AATT_G
Cuts at: 2250 2941 7353 2250
Size: 691 4412 4651
Fragments arranged by size:
4651 4412 691
MluI A'CGCG_T
Cuts at: 9630 9630
Size: 9754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2620 2754 5722 5805 8868 9052 1305
Size: 1315 134 2968 83 3063 184 2007
Fragments arranged by size:
3063 2968 2007 1315 184 134 83
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7932
Fragments arranged by size:
7932 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 5003 5975 6512 7712 8071 8230
Size: 648 1713 2124 972 537 1200 359 159
Cuts at: 8230 518
Size: 2042
Fragments arranged by size:
2124 2042 1713 1200 972 648 537 359
159
MspA1I CmG'CkG
Cuts at: 1606 3350 5308 7170 7847 8995 9240 1606
Size: 1744 1958 1862 677 1148 245 2120
Fragments arranged by size:
2120 1958 1862 1744 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3508 513
Size: 552 2443 6759
Fragments arranged by size:
6759 2443 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9754
NotI GC'GGCC_GC
Cuts at: 7201 7201
Size: 9754
NsiI A_TGCA'T
Cuts at: 2082 6374 2082
Size: 4292 5462
NspI r_CATG'y
Cuts at: 1132 1229 3861 4061 4217 7112 8657 1132
Size: 97 2632 200 156 2895 1545 2229
Fragments arranged by size:
2895 2632 2229 1545 200 156 97
PacI TTA_AT'TAA
Cuts at: 6906 6906
Size: 9754
PasI CC'CwG_GG
Cuts at: 1348 5255 1348
Size: 3907 5847
PciI A'CATG_T
Cuts at: 3857 8653 3857
Size: 4796 4958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3089 4195 4837 1108
Size: 1981 1106 642 6025
Fragments arranged by size:
6025 1981 1106 642
PfoI T'CCnGG_A
Cuts at: 2336 3710 2336
Size: 1374 8380
PmeI GTTT'AAAC
Cuts at: 7058 7058
Size: 9754
PmlI CAC'GTG
Cuts at: 3332 3332
Size: 9754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7823 7855 9336 9368 7823
Size: 32 1481 32 8209
Fragments arranged by size:
8209 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5651 5651
Size: 9754
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9754
PsiI TTA'TAA
Cuts at: 3705 7324 3705
Size: 3619 6135
PstI C_TGCA'G
Cuts at: 830 4501 7106 830
Size: 3671 2605 3478
Fragments arranged by size:
3671 3478 2605
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9754
PvuII CAG'CTG
Cuts at: 3350 5308 3350
Size: 1958 7796
SalI G'TCGA_C
Cuts at: 1936 7096 1936
Size: 5160 4594
SapI GCTCTTCn'nnn_
Cuts at: 5004 5592 7038 5004
Size: 588 1446 7720
Fragments arranged by size:
7720 1446 588
SbfI CC_TGCA'GG
Cuts at: 7106 7106
Size: 9754
ScaI AGT'ACT
Cuts at: 8002 9714 8002
Size: 1712 8042
SfcI C'TryA_G
Cuts at: 826 1045 4497 4681 6078 7102 8237 8918 9109
Size: 219 3452 184 1397 1024 1135 681 191
Cuts at: 9109 826
Size: 1471
Fragments arranged by size:
3452 1471 1397 1135 1024 681 219 191
184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9754
SmaI CCC'GGG
Cuts at: 1872 5870 7081 1872
Size: 3998 1211 4545
Fragments arranged by size:
4545 3998 1211
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2683 5288 6261 7858 8759 9021
Size: 197 926 740 2605 973 1597 901 262
Cuts at: 9021 820
Size: 1553
Fragments arranged by size:
2605 1597 1553 973 926 901 740 262
197
SnaBI TAC'GTA
Cuts at: 493 7545 493
Size: 7052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9754
SphI G_CATG'C
Cuts at: 1132 7112 1132
Size: 5980 3774
SspI AAT'ATT
Cuts at: 5 52 2305 5808 6026 6739 7678 5
Size: 47 2253 3503 218 713 939 2081
Fragments arranged by size:
3503 2253 2081 939 713 218 47
StuI AGG'CCT
Cuts at: 1517 4943 6992 1517
Size: 3426 2049 4279
Fragments arranged by size:
4279 3426 2049
StyI C'CwwG_G
Cuts at: 513 1065 3508 3596 5127 5423 6104 7193 513
Size: 552 2443 88 1531 296 681 1089 3074
Fragments arranged by size:
3074 2443 1531 1089 681 552 296 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4183 4968 5268 7781 7798 7951 8136
Size: 1255 2603 785 300 2513 17 153 185
Cuts at: 8136 8581 325
Size: 445 1498
Fragments arranged by size:
2603 2513 1498 1255 785 445 300 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 4324 4555
Size: 274 80 33 51 1443 1877 470 231
Cuts at: 4555 5142 6889 8000 9712 96
Size: 587 1747 1111 1712 138
Fragments arranged by size:
1877 1747 1712 1443 1111 587 470 274
231 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3129 3161 3210 3242 6401 6433 9714 9746 3129
Size: 32 49 32 3159 32 3281 32 3137
Fragments arranged by size:
3281 3159 3137 49 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9754
XbaI T'CTAG_A
Cuts at: 7090 7090
Size: 9754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3515 4517 3515
Size: 1002 8752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9754
XmaI C'CCGG_G
Cuts at: 1870 5868 7079 1870
Size: 3998 1211 4545
Fragments arranged by size:
4545 3998 1211
XmnI GAAnn'nnTTC
Cuts at: 2711 3312 5971 7883 2711
Size: 601 2659 1912 4582
Fragments arranged by size:
4582 2659 1912 601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7562 276
Size: 53 83 186 936 6028 2468
Fragments arranged by size:
6028 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI