Restriction map for
pFN21AE4286
(Circular) (Six-base) MAPSORT of: pFN21AE4286.seq Check: 7894 from: 1 to: 9598
pFN21AE4286 9598 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7408 278
Size: 53 83 186 936 5872 2468
Fragments arranged by size:
5872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 2745 6941 1937
Size: 484 324 4196 4594
Fragments arranged by size:
4594 4196 484 324
Acc65I G'GTAC_C
Cuts at: 2280 3003 3696 6919 2280
Size: 723 693 3223 4959
Fragments arranged by size:
4959 3223 723 693
AclI AA'CG_TT
Cuts at: 1563 7725 8098 1563
Size: 6162 373 3063
Fragments arranged by size:
6162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 2554 2952 4649 5477 6664 7661 9045
Size: 536 386 398 1697 828 1187 997 1384
Cuts at: 9045 9313 1632
Size: 268 1917
Fragments arranged by size:
1917 1697 1384 1187 997 828 536 398
386 268
AfeI AGC'GCT
Cuts at: 2129 2129
Size: 9598
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9401
AflIII A'CryG_T
Cuts at: 1203 8497 9474 1203
Size: 7294 977 1327
Fragments arranged by size:
7294 1327 977
AgeI A'CCGG_T
Cuts at: 8429 8429
Size: 9598
AhdI GACnn_n'nnGTC
Cuts at: 8327 8327
Size: 9598
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2106 4269 1184
Size: 922 2163 6513
Fragments arranged by size:
6513 2163 922
AlwNI CAG_nnn'CTG
Cuts at: 2104 3049 4675 5228 6074 6484 6631 7341 8913
Size: 945 1626 553 846 410 147 710 1572
Cuts at: 8913 2104
Size: 2789
Fragments arranged by size:
2789 1626 1572 945 846 710 553 410
147
ApaI G_GGCC'C
Cuts at: 2027 3364 4408 2027
Size: 1337 1044 7217
Fragments arranged by size:
7217 1337 1044
ApaLI G'TGCA_C
Cuts at: 7655 8811 7655
Size: 1156 8442
ApoI r'AATT_y
Cuts at: 1493 2501 5421 5477 6907 7133 7285 9394 1493
Size: 1008 2920 56 1430 226 152 2109 1697
Fragments arranged by size:
2920 2109 1697 1430 1008 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 8152 160
Size: 872 7120 1606
Fragments arranged by size:
7120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9598
AvaI C'yCGr_G
Cuts at: 1870 1943 2412 3372 4122 5330 6923 1870
Size: 73 469 960 750 1208 1593 4545
Fragments arranged by size:
4545 1593 1208 960 750 469 73
BamHI G'GATC_C
Cuts at: 3748 6928 7295 3748
Size: 3180 367 6051
Fragments arranged by size:
6051 3180 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2280 3003 3696 5770 6919
Size: 325 217 627 493 723 693 2074 1149
Cuts at: 6919 8374 618
Size: 1455 1842
Fragments arranged by size:
2074 1842 1455 1149 723 693 627 493
325 217
BanII G_rGCy'C
Cuts at: 1391 2027 2604 2893 2930 3315 3364 3964 4408
Size: 636 577 289 37 385 49 600 444
Cuts at: 4408 5605 5937 6917 1391
Size: 1197 332 980 4072
Fragments arranged by size:
4072 1197 980 636 600 577 444 385
332 289 49 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3635 5205 928
Size: 971 1736 1570 5321
Fragments arranged by size:
5321 1736 1570 971
BbvCI CC'TCA_GC
Cuts at: 2058 2511 3426 4614 2058
Size: 453 915 1188 7042
Fragments arranged by size:
7042 1188 915 453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5353 5387 7789 7823 1903
Size: 34 3416 34 2402 34 3678
Fragments arranged by size:
3678 3416 2402 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7490 8700 7490
Size: 1210 8388
BclI T'GATC_A
Cuts at: 1548 3118 5911 1548
Size: 1570 2793 5235
Fragments arranged by size:
5235 2793 1570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2888 3368 4230
Size: 107 122 71 631 605 1216 480 862
Cuts at: 4230 5633 8209 136
Size: 1403 2576 1525
Fragments arranged by size:
2576 1525 1403 1216 862 631 605 480
122 107 71
BglII A'GATC_T
Cuts at: 2256 6661 9593 2256
Size: 4405 2932 2261
Fragments arranged by size:
4405 2932 2261
BlpI GC'TnA_GC
Cuts at: 4257 4838 5210 5643 6624 7015 4257
Size: 581 372 433 981 391 6840
Fragments arranged by size:
6840 981 581 433 391 372
Bme1580I G_kGCm'C
Cuts at: 1790 2027 3212 3364 4408 5158 6006 7659 8815
Size: 237 1185 152 1044 750 848 1653 1156
Cuts at: 8815 1790
Size: 2573
Fragments arranged by size:
2573 1653 1185 1156 1044 848 750 237
152
BmgBI CAC'GTC
Cuts at: 1318 5086 1318
Size: 3768 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2697 7329 8287
Size: 416 53 83 399 24 1280 4632 958
Cuts at: 8287 442
Size: 1753
Fragments arranged by size:
4632 1753 1280 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9598
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3352 3849 3352
Size: 497 9101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2198 2540 2735 3029 3213 3263 3284
Size: 896 63 342 195 294 184 50 21
Cuts at: 3284 3386 3412 3774 4199 4367 4619 4697 4877
Size: 102 26 362 425 168 252 78 180
Cuts at: 4877 4981 5009 5030 5741 5906 5960 6785 8258
Size: 104 28 21 711 165 54 825 1473
Cuts at: 8258 1239
Size: 2579
Fragments arranged by size:
2579 1473 896 825 711 425 362 342
294 252 195 184 180 168 165 104
102 78 63 54 50 28 26 21
21
Bpu10I CC'TnA_GC
Cuts at: 2058 2511 3426 3509 4614 6014 6485 2058
Size: 453 915 83 1105 1400 471 5171
Fragments arranged by size:
5171 1400 1105 915 471 453 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3791 5341 5720 6143 7723 8588 8886 2927
Size: 864 1550 379 423 1580 865 298 3639
Fragments arranged by size:
3639 1580 1550 865 864 423 379 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 4756 8261 882
Size: 617 873 2384 3505 2219
Fragments arranged by size:
3505 2384 2219 873 617
BsaAI yAC'GTr
Cuts at: 493 1204 7389 493
Size: 711 6185 2702
Fragments arranged by size:
6185 2702 711
BsaBI GATnn'nnATC
Cuts at: 9592 9592
Size: 9598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2531 4441 5487 5568
Size: 53 83 186 936 998 1910 1046 81
Cuts at: 5568 6226 7405 7787 8422 275
Size: 658 1179 382 635 1451
Fragments arranged by size:
1910 1451 1179 1046 998 936 658 635
382 186 83 81 53
BsaWI w'CCGG_w
Cuts at: 3168 4728 6250 8031 8429 8703 8850 9261 3168
Size: 1560 1522 1781 398 274 147 411 3505
Fragments arranged by size:
3505 1781 1560 1522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3191 3221 4121 4151 4979
Size: 30 165 30 1788 30 900 30 828
Cuts at: 4979 5009 5352 5382 6155 6185 1178
Size: 30 343 30 773 30 4591
Fragments arranged by size:
4591 1788 900 828 773 343 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2075 2132 2168 2195 2998 3323 3695
Size: 840 49 57 36 27 803 325 372
Cuts at: 3695 3788 4142 4542 5360 5798 6524 6863 1186
Size: 93 354 400 818 438 726 339 3921
Fragments arranged by size:
3921 840 818 803 726 438 400 372
354 339 325 93 57 49 36 27
BseYI C'CCAG_C
Cuts at: 2031 2215 2458 3049 3219 3447 4288 5548 5616
Size: 184 243 591 170 228 841 1260 68
Cuts at: 5616 8801 9297 2031
Size: 3185 496 2332
Fragments arranged by size:
3185 2332 1260 841 591 496 243 228
184 170 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3167 4430 1163
Size: 573 1431 1263 6331
Fragments arranged by size:
6331 1431 1263 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7048 7809 8837 1540
Size: 275 186 5047 761 1028 2301
Fragments arranged by size:
5047 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2604 2710 3279 3315 5211 5892 5937 6110 6491
Size: 106 569 36 1896 681 45 173 381
Cuts at: 6491 7659 7744 8815 2604
Size: 1168 85 1071 3387
Fragments arranged by size:
3387 1896 1168 1071 681 569 381 173
106 85 45 36
BsmI GAATG_Cn'
Cuts at: 4261 7109 7202 4261
Size: 2848 93 6657
Fragments arranged by size:
6657 2848 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5335 5335
Size: 9598
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2604 2710 2893 2930 3212 3279
Size: 399 237 577 106 183 37 282 67
Cuts at: 3279 3315 3364 3964 4408 5158 5211 5605 5892
Size: 36 49 600 444 750 53 394 287
Cuts at: 5892 5937 6006 6110 6491 6917 7659 7744 8815
Size: 45 69 104 381 426 742 85 1071
Cuts at: 8815 1391
Size: 2174
Fragments arranged by size:
2174 1071 750 742 600 577 444 426
399 394 381 287 282 237 183 106
104 85 69 67 53 49 45 37
36
BspEI T'CCGG_A
Cuts at: 3168 9261 3168
Size: 6093 3505
BspHI T'CATG_A
Cuts at: 6822 7487 9217 6822
Size: 665 1730 7203
Fragments arranged by size:
7203 1730 665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3531 4896 6342 6953 844
Size: 2687 1365 1446 611 3489
Fragments arranged by size:
3489 2687 1446 1365 611
BsrBI CCG'CTC
Cuts at: 1394 1823 3892 6273 7485 1394
Size: 429 2069 2381 1212 3507
Fragments arranged by size:
3507 2381 2069 1212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5901 8093 8267 65
Size: 1183 4653 2192 174 1396
Fragments arranged by size:
4653 2192 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 8242 8429 1700
Size: 110 299 6133 187 2869
Fragments arranged by size:
6133 2869 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 4983 5841 6236 96
Size: 4887 858 395 3458
Fragments arranged by size:
4887 3458 858 395
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9598
BssSI C'ACGA_G
Cuts at: 7658 8670 7658
Size: 1012 8586
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2627 2789 794
Size: 1833 162 7603
Fragments arranged by size:
7603 1833 162
BstBI TT'CG_AA
Cuts at: 8402 8402
Size: 9598
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9598
BstXI CCAn_nnnn'nTGG
Cuts at: 2291 3421 4912 4960 5836 6748 2291
Size: 1130 1491 48 876 912 5141
Fragments arranged by size:
5141 1491 1130 912 876 48
BstYI r'GATC_y
Cuts at: 1972 2256 3748 3777 4074 4131 4302 4392 4482
Size: 284 1492 29 297 57 171 90 90
Cuts at: 4482 4656 5944 6165 6661 6787 6928 7295 7680
Size: 174 1288 221 496 126 141 367 385
Cuts at: 7680 7697 9149 9235 9247 9593 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1492 1452 1288 496 385 367 346
297 284 221 174 171 141 126 90
90 86 57 29 17 12
Bsu36I CC'TnA_GG
Cuts at: 3213 4436 3213
Size: 1223 8375
BtgI C'CryG_G
Cuts at: 513 1065 2004 2238 2382 2884 3975 4228 5586
Size: 552 939 234 144 502 1091 253 1358
Cuts at: 5586 5835 5850 513
Size: 249 15 4261
Fragments arranged by size:
4261 1358 1091 939 552 502 253 249
234 144 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5338 5974 487
Size: 683 56 4112 636 4111
Fragments arranged by size:
4112 4111 683 636 56
BtsI GCAGTG_nn'
Cuts at: 2661 3279 3918 4038 4155 4756 4974 5802 6257
Size: 618 639 120 117 601 218 828 455
Cuts at: 6257 6615 7114 7908 7928 2661
Size: 358 499 794 20 4331
Fragments arranged by size:
4331 828 794 639 618 601 499 455
358 218 120 117 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9563
DraI TTT'AAA
Cuts at: 4366 4830 6902 4366
Size: 464 2072 7062
Fragments arranged by size:
7062 2072 464
DraIII CAC_nnn'GTG
Cuts at: 3145 3145
Size: 9598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5501 8605 809
Size: 507 4185 3104 1802
Fragments arranged by size:
4185 3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2541 2592
Size: 54 1425 50 275 18 378 333 51
Cuts at: 2592 3301 4914 5445 5583 5826 6123 6402 7045
Size: 709 1613 531 138 243 297 279 643
Cuts at: 7045 7934 9563 8
Size: 889 1629 43
Fragments arranged by size:
1629 1613 1425 889 709 643 531 378
333 297 279 275 243 138 54 51
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7045 1537
Size: 275 5233 4090
Fragments arranged by size:
5233 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2450 2456 2715 3320 3558 4635 4886 6612
Size: 1097 6 259 605 238 1077 251 1726
Cuts at: 6612 6836 7528 1353
Size: 224 692 3423
Fragments arranged by size:
3423 1726 1097 1077 692 605 259 251
238 224 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3770 6457 8185 8559 8705 387
Size: 1064 138 2181 2687 1728 374 146 1280
Fragments arranged by size:
2687 2181 1728 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2602 3313 5935 2602
Size: 711 2622 6265
Fragments arranged by size:
6265 2622 711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2168 2198 2540 2554 2735 2952
Size: 393 503 33 30 342 14 181 217
Cuts at: 2952 3029 3213 3263 3284 3386 3412 3774 4199
Size: 77 184 50 21 102 26 362 425
Cuts at: 4199 4367 4619 4649 4697 4877 4981 5009 5030
Size: 168 252 30 48 180 104 28 21
Cuts at: 5030 5477 5741 5906 5960 6664 6785 7661 8258
Size: 447 264 165 54 704 121 876 597
Cuts at: 8258 9045 9313 1239
Size: 787 268 1524
Fragments arranged by size:
1524 876 787 704 597 503 447 425
393 362 342 268 264 252 217 184
181 180 168 165 121 104 102 77
54 50 48 33 30 30 28 26
21 21 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2843 3414 3425 6635 2150
Size: 693 571 11 3210 5113
Fragments arranged by size:
5113 3210 693 571 11
EcoO109I rG'GnC_Cy
Cuts at: 2947 3361 3577 4278 4684 5704 6481 2947
Size: 414 216 701 406 1020 777 6064
Fragments arranged by size:
6064 1020 777 701 414 406 216
EcoRI G'AATT_C
Cuts at: 6907 7285 6907
Size: 378 9220
EcoRV GAT'ATC
Cuts at: 6130 6130
Size: 9598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4942 6517 6661 4942
Size: 1575 144 7879
Fragments arranged by size:
7879 1575 144
FspI TGC'GCA
Cuts at: 8104 8104
Size: 9598
HaeII r_GCGC'y
Cuts at: 1678 2131 5623 6369 8745 1678
Size: 453 3492 746 2376 2531
Fragments arranged by size:
3492 2531 2376 746 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2467 2499 3733 3765 4121
Size: 32 135 32 895 32 1234 32 356
Cuts at: 4121 4153 4294 4326 5337 5369 5792 5824 6155
Size: 32 141 32 1011 32 423 32 331
Cuts at: 6155 6187 8239 8271 8313 8345 1373
Size: 32 2052 32 42 32 2626
Fragments arranged by size:
2626 2052 1234 1011 895 423 356 331
141 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 4468 5866 6856 6942 7188
Size: 988 281 940 1590 1398 990 86 246
Cuts at: 7188 669
Size: 3079
Fragments arranged by size:
3079 1590 1398 990 988 940 281 246
86
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9598
HpaI GTT'AAC
Cuts at: 4468 7188 4468
Size: 2720 6878
KpnI G_GTAC'C
Cuts at: 2284 3007 3700 6923 2284
Size: 723 693 3223 4959
Fragments arranged by size:
4959 3223 723 693
MfeI C'AATT_G
Cuts at: 7197 7197
Size: 9598
MluI A'CGCG_T
Cuts at: 9474 9474
Size: 9598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6108 8712 8896 1305
Size: 4803 2604 184 2007
Fragments arranged by size:
4803 2604 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2543 2594 3303 4916
Size: 54 1425 343 378 333 51 709 1613
Cuts at: 4916 5447 5585 5828 6404 10
Size: 531 138 243 576 3204
Fragments arranged by size:
3204 1613 1425 709 576 531 378 343
333 243 138 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 5849 7556 7915 8074 518
Size: 648 4683 1707 359 159 2042
Fragments arranged by size:
4683 2042 1707 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 2057 2275 2510 2560 2633 3016 3151
Size: 415 36 218 235 50 73 383 135
Cuts at: 3151 3223 3262 3341 3659 4142 4333 4675 4792
Size: 72 39 79 318 483 191 342 117
Cuts at: 4792 5029 5552 5594 6118 6214 6316 7014 7691
Size: 237 523 42 524 96 102 698 677
Cuts at: 7691 8839 9084 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 524 523 483 415
383 342 318 245 237 235 218 191
135 117 102 96 79 73 72 50
42 39 36
NaeI GCC'GGC
Cuts at: 2111 2111
Size: 9598
NcoI C'CATG_G
Cuts at: 513 1065 2004 2884 3975 5586 5835 513
Size: 552 939 880 1091 1611 249 4276
Fragments arranged by size:
4276 1611 1091 939 880 552 249
NdeI CA'TA_TG
Cuts at: 387 5296 387
Size: 4909 4689
NgoMIV G'CCGG_C
Cuts at: 2109 2109
Size: 9598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9598
NotI GC'GGCC_GC
Cuts at: 7045 7045
Size: 9598
NspI r_CATG'y
Cuts at: 1132 1229 2410 4541 6956 8501 1132
Size: 97 1181 2131 2415 1545 2229
Fragments arranged by size:
2415 2229 2131 1545 1181 97
PasI CC'CwG_GG
Cuts at: 1348 5629 6352 1348
Size: 4281 723 4594
Fragments arranged by size:
4594 4281 723
PciI A'CATG_T
Cuts at: 8497 8497
Size: 9598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2130 3790 5086 5629 1108
Size: 1022 1660 1296 543 5077
Fragments arranged by size:
5077 1660 1296 1022 543
PfoI T'CCnGG_A
Cuts at: 3751 4635 5046 5184 5937 6630 3751
Size: 884 411 138 753 693 6719
Fragments arranged by size:
6719 884 753 693 411 138
PmeI GTTT'AAAC
Cuts at: 6902 6902
Size: 9598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3915 3947 7667 7699 9180 9212 3915
Size: 32 3720 32 1481 32 4301
Fragments arranged by size:
4301 3720 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3577 4278 4684 5704 3577
Size: 701 406 1020 7471
Fragments arranged by size:
7471 1020 701 406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9598
PsiI TTA'TAA
Cuts at: 7168 7168
Size: 9598
PspOMI G'GGCC_C
Cuts at: 2023 3360 4404 2023
Size: 1337 1044 7217
Fragments arranged by size:
7217 1337 1044
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5513 5545 5513
Size: 32 9566
PstI C_TGCA'G
Cuts at: 830 2623 3103 3528 3658 4062 4743 5163 5998
Size: 1793 480 425 130 404 681 420 835
Cuts at: 5998 6468 6798 6950 830
Size: 470 330 152 3478
Fragments arranged by size:
3478 1793 835 681 480 470 425 420
404 330 152 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9598
PvuII CAG'CTG
Cuts at: 2057 2275 2510 2560 2633 3016 3223 3262 3341
Size: 218 235 50 73 383 207 39 79
Cuts at: 3341 3659 4142 4675 4792 5029 5552 5594 6118
Size: 318 483 533 117 237 523 42 524
Cuts at: 6118 6214 2057
Size: 96 5441
Fragments arranged by size:
5441 533 524 523 483 383 318 237
235 218 207 117 96 79 73 50
42 39
SacI G_AGCT'C
Cuts at: 2604 3315 5937 2604
Size: 711 2622 6265
Fragments arranged by size:
6265 2622 711
SalI G'TCGA_C
Cuts at: 1936 6940 1936
Size: 5004 4594
SapI GCTCTTCn'nnn_
Cuts at: 2715 3320 2715
Size: 605 8993
SbfI CC_TGCA'GG
Cuts at: 3528 5163 6950 3528
Size: 1635 1787 6176
Fragments arranged by size:
6176 1787 1635
ScaI AGT'ACT
Cuts at: 5807 7846 9558 5807
Size: 2039 1712 5847
Fragments arranged by size:
5847 2039 1712
SfcI C'TryA_G
Cuts at: 826 1045 2619 3099 3524 3654 4058 4522 4739
Size: 219 1574 480 425 130 404 464 217
Cuts at: 4739 5159 5651 5994 6464 6526 6794 6946 8081
Size: 420 492 343 470 62 268 152 1135
Cuts at: 8081 8762 8953 826
Size: 681 191 1471
Fragments arranged by size:
1574 1471 1135 681 492 480 470 464
425 420 404 343 268 219 217 191
152 130 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 3368 3368
Size: 9598
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9598
SmaI CCC'GGG
Cuts at: 1872 2414 3374 4124 6925 1872
Size: 542 960 750 2801 4545
Fragments arranged by size:
4545 2801 960 750 542
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2942 3770 5320 5735 6158 7702
Size: 197 926 999 828 1550 415 423 1544
Cuts at: 7702 8603 8865 820
Size: 901 262 1553
Fragments arranged by size:
1553 1550 1544 999 926 901 828 423
415 262 197
SnaBI TAC'GTA
Cuts at: 493 7389 493
Size: 6896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9598
SphI G_CATG'C
Cuts at: 1132 4541 6956 1132
Size: 3409 2415 3774
Fragments arranged by size:
3774 3409 2415
SspI AAT'ATT
Cuts at: 5 52 7522 5
Size: 47 7470 2081
Fragments arranged by size:
7470 2081 47
StuI AGG'CCT
Cuts at: 1517 2141 3420 4400 5012 5318 5675 6233 1517
Size: 624 1279 980 612 306 357 558 4882
Fragments arranged by size:
4882 1279 980 624 612 558 357 306
StyI C'CwwG_G
Cuts at: 513 1065 2004 2884 3975 4149 5586 5835 6405
Size: 552 939 880 1091 174 1437 249 570
Cuts at: 6405 7037 513
Size: 632 3074
Fragments arranged by size:
3074 1437 1091 939 880 632 570 552
249 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 3485 6740 7625 7642 7795 7980
Size: 1255 762 1143 3255 885 17 153 185
Cuts at: 7980 8425 325
Size: 445 1498
Fragments arranged by size:
3255 1498 1255 1143 885 762 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4983 5529 5805
Size: 274 80 33 51 1443 3006 546 276
Cuts at: 5805 5841 6236 6534 7844 9556 96
Size: 36 395 298 1310 1712 138
Fragments arranged by size:
3006 1712 1443 1310 546 395 298 276
274 138 80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 9558 9590 3191
Size: 32 6335 32 3199
Fragments arranged by size:
6335 3199 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3575 3634 5228 1316
Size: 2259 59 1594 5686
Fragments arranged by size:
5686 2259 1594 59
XbaI T'CTAG_A
Cuts at: 2893 6934 2893
Size: 4041 5557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2710 4672 4957 5426 6118 2131
Size: 579 1962 285 469 692 5611
Fragments arranged by size:
5611 1962 692 579 469 285
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9598
XmaI C'CCGG_G
Cuts at: 1870 2412 3372 4122 6923 1870
Size: 542 960 750 2801 4545
Fragments arranged by size:
4545 2801 960 750 542
XmnI GAAnn'nnTTC
Cuts at: 6663 6830 7727 6663
Size: 167 897 8534
Fragments arranged by size:
8534 897 167
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7406 276
Size: 53 83 186 936 5872 2468
Fragments arranged by size:
5872 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI RsrII SacII SanDI SexAI SfoI SrfI
SwaI