Restriction map for
pFN21AE4277
(Circular) (Six-base) MAPSORT of: pFN21AE4277.seq Check: 6216 from: 1 to: 8545
pFN21AE4277 8545 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6355 278
Size: 53 83 186 936 4819 2468
Fragments arranged by size:
4819 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2488 5888 1937
Size: 551 3400 4594
Fragments arranged by size:
4594 3400 551
Acc65I G'GTAC_C
Cuts at: 2464 5866 2464
Size: 3402 5143
AclI AA'CG_TT
Cuts at: 1563 6672 7045 1563
Size: 5109 373 3063
Fragments arranged by size:
5109 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2560 2845 3088 3535 4429 4739 5435 5639
Size: 928 285 243 447 894 310 696 204
Cuts at: 5639 6608 7992 8260 1632
Size: 969 1384 268 1917
Fragments arranged by size:
1917 1384 969 928 894 696 447 310
285 268 243 204
AfeI AGC'GCT
Cuts at: 4085 4085
Size: 8545
AflII C'TTAA_G
Cuts at: 820 1017 2842 820
Size: 197 1825 6523
Fragments arranged by size:
6523 1825 197
AflIII A'CryG_T
Cuts at: 1203 7444 8421 1203
Size: 6241 977 1327
Fragments arranged by size:
6241 1327 977
AgeI A'CCGG_T
Cuts at: 7376 7376
Size: 8545
AhdI GACnn_n'nnGTC
Cuts at: 7274 7274
Size: 8545
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8545
AlwNI CAG_nnn'CTG
Cuts at: 3829 3997 4303 5497 5841 6288 7860 3829
Size: 168 306 1194 344 447 1572 4514
Fragments arranged by size:
4514 1572 1194 447 344 306 168
ApaLI G'TGCA_C
Cuts at: 3455 4701 6602 7758 3455
Size: 1246 1901 1156 4242
Fragments arranged by size:
4242 1901 1246 1156
ApoI r'AATT_y
Cuts at: 1493 3098 3356 3365 5825 5854 6080 6232 8341
Size: 1605 258 9 2460 29 226 152 2109
Cuts at: 8341 1493
Size: 1697
Fragments arranged by size:
2460 2109 1697 1605 258 226 152 29
9
AseI AT'TA_AT
Cuts at: 160 1032 7099 160
Size: 872 6067 1606
Fragments arranged by size:
6067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8545
AvaI C'yCGr_G
Cuts at: 1870 1943 2429 5067 5870 1870
Size: 73 486 2638 803 4545
Fragments arranged by size:
4545 2638 803 486 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3596 3629 3596
Size: 33 8512
BamHI G'GATC_C
Cuts at: 2157 3679 5875 6242 2157
Size: 1522 2196 367 4460
Fragments arranged by size:
4460 2196 1522 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 2464 4975 5063 5866
Size: 325 217 627 660 17 2511 88 803
Cuts at: 5866 7321 618
Size: 1455 1842
Fragments arranged by size:
2511 1842 1455 803 660 627 325 217
88 17
BanII G_rGCy'C
Cuts at: 1391 2013 2735 5213 5864 1391
Size: 622 722 2478 651 4072
Fragments arranged by size:
4072 2478 722 651 622
BbeI G_GCGC'C
Cuts at: 2451 2451
Size: 8545
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4386 4890 5554 928
Size: 971 2487 504 664 3919
Fragments arranged by size:
3919 2487 971 664 504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6736 6770 1903
Size: 34 4799 34 3678
Fragments arranged by size:
4799 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3258 3951 6437 7647 3258
Size: 693 2486 1210 4156
Fragments arranged by size:
4156 2486 1210 693
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8545
BfrBI ATG'CAT
Cuts at: 4595 4595
Size: 8545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2592 5150
Size: 107 122 71 631 605 367 553 2558
Cuts at: 5150 7156 136
Size: 2006 1525
Fragments arranged by size:
2558 2006 1525 631 605 553 367 122
107 71
BglII A'GATC_T
Cuts at: 4098 8540 4098
Size: 4442 4103
BlpI GC'TnA_GC
Cuts at: 2729 5962 2729
Size: 3233 5312
Bme1580I G_kGCm'C
Cuts at: 1790 3459 4705 5068 6606 7762 1790
Size: 1669 1246 363 1538 1156 2573
Fragments arranged by size:
2573 1669 1538 1246 1156 363
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 3638 3909
Size: 416 53 83 399 24 697 1524 271
Cuts at: 3909 5031 5285 6276 7234 442
Size: 1122 254 991 958 1753
Fragments arranged by size:
1753 1524 1122 991 958 697 416 399
271 254 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 4975 2098
Size: 2877 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2186 2480 4312 4547 4945 5185 5468
Size: 854 93 294 1832 235 398 240 283
Cuts at: 5468 5543 7205 1239
Size: 75 1662 2579
Fragments arranged by size:
2579 1832 1662 854 398 294 283 240
235 93 75
Bpu10I CC'TnA_GC
Cuts at: 4847 4847
Size: 8545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3425 3578 4566 6670 7535 7833 3425
Size: 153 988 2104 865 298 4137
Fragments arranged by size:
4137 2104 988 865 298 153
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2685 7208 882
Size: 617 1186 4523 2219
Fragments arranged by size:
4523 2219 1186 617
BsaAI yAC'GTr
Cuts at: 493 1204 5111 6336 493
Size: 711 3907 1225 2702
Fragments arranged by size:
3907 2702 1225 711
BsaBI GATnn'nnATC
Cuts at: 8539 8539
Size: 8545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2448 6352 6734 7369
Size: 53 83 186 936 915 3904 382 635
Cuts at: 7369 275
Size: 1451
Fragments arranged by size:
3904 1451 936 915 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2024 4628 6978 7376 7650 7797 8208 2024
Size: 2604 2350 398 274 147 411 2361
Fragments arranged by size:
2604 2361 2350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 2159 2189 2267
Size: 30 165 30 627 30 99 30 78
Cuts at: 2267 2297 1178
Size: 30 7426
Fragments arranged by size:
7426 627 165 99 78 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2043 2407 3449 4345 4471 5549 1186
Size: 857 364 1042 896 126 1078 4182
Fragments arranged by size:
4182 1078 1042 896 857 364 126
BseYI C'CCAG_C
Cuts at: 2190 3683 4729 4904 7748 8244 2190
Size: 1493 1046 175 2844 496 2491
Fragments arranged by size:
2844 2491 1493 1046 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4709 5183 5495 1163
Size: 573 2973 474 312 4213
Fragments arranged by size:
4213 2973 573 474 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4221 5995 6756 7784 1540
Size: 275 186 2220 1774 761 1028 2301
Fragments arranged by size:
2301 2220 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 3459 4691 4705 6606 6691 7762 2013
Size: 1446 1232 14 1901 85 1071 2796
Fragments arranged by size:
2796 1901 1446 1232 1071 85 14
BsmI GAATG_Cn'
Cuts at: 4040 4177 6056 6149 4040
Size: 137 1879 93 6436
Fragments arranged by size:
6436 1879 137 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4300 4552 4300
Size: 252 8293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2735 3459 4691 4705 5068 5213
Size: 399 223 722 724 1232 14 363 145
Cuts at: 5213 5864 6606 6691 7762 1391
Size: 651 742 85 1071 2174
Fragments arranged by size:
2174 1232 1071 742 724 722 651 399
363 223 145 85 14
BspEI T'CCGG_A
Cuts at: 2024 8208 2024
Size: 6184 2361
BspHI T'CATG_A
Cuts at: 3619 4044 6434 8164 3619
Size: 425 2390 1730 4000
Fragments arranged by size:
4000 2390 1730 425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5245 5755 5900 844
Size: 4401 510 145 3489
Fragments arranged by size:
4401 3489 510 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2278 5539 5752 6432 1394
Size: 429 455 3261 213 680 3507
Fragments arranged by size:
3507 3261 680 455 429 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 4815 5779 7040 7214 65
Size: 1183 3567 964 1261 174 1396
Fragments arranged by size:
3567 1396 1261 1183 964 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2138 5175 5439 7189 7376 1700
Size: 110 328 3037 264 1750 187 2869
Fragments arranged by size:
3037 2869 1750 328 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8545
BssHII G'CGCG_C
Cuts at: 1925 5730 1925
Size: 3805 4740
BssSI C'ACGA_G
Cuts at: 2638 2682 3786 4245 6605 7617 2638
Size: 44 1104 459 2360 1012 3566
Fragments arranged by size:
3566 2360 1104 1012 459 44
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4975 5488 5497 794
Size: 4181 513 9 3842
Fragments arranged by size:
4181 3842 513 9
BstBI TT'CG_AA
Cuts at: 7349 7349
Size: 8545
BstEII G'GTnAC_C
Cuts at: 1184 2833 1184
Size: 1649 6896
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2533 4748 2458
Size: 75 2215 6255
Fragments arranged by size:
6255 2215 75
BstYI r'GATC_y
Cuts at: 1972 2157 2758 3679 4098 5034 5875 6242 6627
Size: 185 601 921 419 936 841 367 385
Cuts at: 6627 6644 8096 8182 8194 8540 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 936 921 841 601 419 385
367 346 185 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2390 2390
Size: 8545
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2532 3835 4221 513
Size: 552 939 30 498 1303 386 4837
Fragments arranged by size:
4837 1303 939 552 498 386 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2398 487
Size: 683 56 1172 6634
Fragments arranged by size:
6634 1172 683 56
BtsI GCAGTG_nn'
Cuts at: 2538 3207 3830 4851 6061 6855 6875 2538
Size: 669 623 1021 1210 794 20 4208
Fragments arranged by size:
4208 1210 1021 794 669 623 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4879 4914 529
Size: 35 4315 35 4160
Fragments arranged by size:
4315 4160 35 35
DraI TTT'AAA
Cuts at: 5849 5849
Size: 8545
DraIII CAC_nnn'GTG
Cuts at: 4975 5099 4975
Size: 124 8421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2114 7552 809
Size: 507 798 5438 1802
Fragments arranged by size:
5438 1802 798 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2051 3999 4656
Size: 54 1425 50 275 18 221 1948 657
Cuts at: 4656 5151 5992 6881 8510 8
Size: 495 841 889 1629 43
Fragments arranged by size:
1948 1629 1425 889 841 657 495 275
221 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5992 1537
Size: 275 4180 4090
Fragments arranged by size:
4180 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3123 5200 5331 5604 6475 1353
Size: 1770 2077 131 273 871 3423
Fragments arranged by size:
3423 2077 1770 871 273 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7132 7506 7652 387
Size: 1064 138 5543 374 146 1280
Fragments arranged by size:
5543 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 2011
Size: 8545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2186 2480 2560 2845 3088 3535
Size: 393 461 93 294 80 285 243 447
Cuts at: 3535 4312 4429 4547 4739 4945 5185 5435 5468
Size: 777 117 118 192 206 240 250 33
Cuts at: 5468 5543 5639 6608 7205 7992 8260 1239
Size: 75 96 969 597 787 268 1524
Fragments arranged by size:
1524 969 787 777 597 461 447 393
294 285 268 250 243 240 206 192
118 117 96 93 80 75 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2343 3932 2343
Size: 1589 6956
EcoO109I rG'GnC_Cy
Cuts at: 2101 2478 2986 3290 4235 4719 2101
Size: 377 508 304 945 484 5927
Fragments arranged by size:
5927 945 508 484 377 304
EcoRI G'AATT_C
Cuts at: 3098 5825 5854 6232 3098
Size: 2727 29 378 5411
Fragments arranged by size:
5411 2727 378 29
FspI TGC'GCA
Cuts at: 7051 7051
Size: 8545
HaeII r_GCGC'y
Cuts at: 1678 2045 2072 2451 2587 4087 5425 7692 1678
Size: 367 27 379 136 1500 1338 2267 2531
Fragments arranged by size:
2531 2267 1500 1338 379 367 136 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2481 2513 2663
Size: 32 135 32 458 32 419 32 150
Cuts at: 2663 2695 3700 3732 3941 3955 3973 3987 4090
Size: 32 1005 32 209 14 18 14 103
Cuts at: 4090 4103 4122 4135 4580 4612 7186 7218 7260
Size: 13 19 13 445 32 2574 32 42
Cuts at: 7260 7292 1373
Size: 32 2626
Fragments arranged by size:
2626 2574 1005 458 445 419 209 150
135 103 42 32 32 32 32 32
32 32 32 32 19 18 14 14
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3199 5889 6135 669
Size: 988 281 1261 2690 246 3079
Fragments arranged by size:
3079 2690 1261 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2624 3645 5801 748
Size: 1876 1021 2156 3492
Fragments arranged by size:
3492 2156 1876 1021
HpaI GTT'AAC
Cuts at: 6135 6135
Size: 8545
KasI G'GCGC_C
Cuts at: 2447 2447
Size: 8545
KpnI G_GTAC'C
Cuts at: 2468 5870 2468
Size: 3402 5143
MfeI C'AATT_G
Cuts at: 2935 6144 2935
Size: 3209 5336
MluI A'CGCG_T
Cuts at: 8421 8421
Size: 8545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 2130 4520 7659 7843 1305
Size: 798 27 2390 3139 184 2007
Fragments arranged by size:
3139 2390 2007 798 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4001 4658 10
Size: 54 1425 343 2169 657 3897
Fragments arranged by size:
3897 2169 1425 657 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5129 6503 6862 7021 518
Size: 648 3963 1374 359 159 2042
Fragments arranged by size:
3963 2042 1374 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 2089 2865 4672 5841 5961 6638 7786
Size: 430 53 776 1807 1169 120 677 1148
Cuts at: 7786 8031 1606
Size: 245 2120
Fragments arranged by size:
2120 1807 1169 1148 776 677 430 245
120 53
NaeI GCC'GGC
Cuts at: 5441 5441
Size: 8545
NarI GG'CG_CC
Cuts at: 2448 2448
Size: 8545
NcoI C'CATG_G
Cuts at: 513 1065 2004 2532 513
Size: 552 939 528 6526
Fragments arranged by size:
6526 939 552 528
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8545
NgoMIV G'CCGG_C
Cuts at: 5439 5439
Size: 8545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8545
NotI GC'GGCC_GC
Cuts at: 5992 5992
Size: 8545
NsiI A_TGCA'T
Cuts at: 4597 4597
Size: 8545
NspI r_CATG'y
Cuts at: 1132 1229 2149 4595 5128 5384 5764 5903 7448
Size: 97 920 2446 533 256 380 139 1545
Cuts at: 7448 1132
Size: 2229
Fragments arranged by size:
2446 2229 1545 920 533 380 256 139
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8545
PciI A'CATG_T
Cuts at: 7444 7444
Size: 8545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2126 1108
Size: 1018 7527
PfoI T'CCnGG_A
Cuts at: 4563 4563
Size: 8545
PmeI GTTT'AAAC
Cuts at: 5849 5849
Size: 8545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6614 6646 8127 8159 6614
Size: 32 1481 32 7000
Fragments arranged by size:
7000 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2478 2986 3290 2101
Size: 377 508 304 7356
Fragments arranged by size:
7356 508 377 304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8545
PsiI TTA'TAA
Cuts at: 6115 6115
Size: 8545
PstI C_TGCA'G
Cuts at: 830 4368 5897 830
Size: 3538 1529 3478
Fragments arranged by size:
3538 3478 1529
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8545
PvuII CAG'CTG
Cuts at: 2865 4672 5841 2865
Size: 1807 1169 5569
Fragments arranged by size:
5569 1807 1169
SacI G_AGCT'C
Cuts at: 2013 2013
Size: 8545
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 8545
SalI G'TCGA_C
Cuts at: 1936 5887 1936
Size: 3951 4594
SbfI CC_TGCA'GG
Cuts at: 5897 5897
Size: 8545
ScaI AGT'ACT
Cuts at: 3472 5020 6793 8505 3472
Size: 1548 1773 1712 3512
Fragments arranged by size:
3512 1773 1712 1548
SfcI C'TryA_G
Cuts at: 826 1045 4364 4824 5893 7028 7709 7900 826
Size: 219 3319 460 1069 1135 681 191 1471
Fragments arranged by size:
3319 1471 1135 1069 681 460 219 191
SfoI GGC'GCC
Cuts at: 2449 2449
Size: 8545
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8545
SgrAI Cr'CCGG_yG
Cuts at: 1700 2138 1700
Size: 438 8107
SmaI CCC'GGG
Cuts at: 1872 5872 1872
Size: 4000 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2842 3404 3593 4581 6649 7550
Size: 197 926 899 562 189 988 2068 901
Cuts at: 7550 7812 820
Size: 262 1553
Fragments arranged by size:
2068 1553 988 926 901 899 562 262
197 189
SnaBI TAC'GTA
Cuts at: 493 5111 6336 493
Size: 4618 1225 2702
Fragments arranged by size:
4618 2702 1225
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8545
SphI G_CATG'C
Cuts at: 1132 4595 5384 5764 5903 1132
Size: 3463 789 380 139 3774
Fragments arranged by size:
3774 3463 789 380 139
SspI AAT'ATT
Cuts at: 5 52 3008 3076 4374 6469 5
Size: 47 2956 68 1298 2095 2081
Fragments arranged by size:
2956 2095 2081 1298 68 47
StuI AGG'CCT
Cuts at: 1517 4820 1517
Size: 3303 5242
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 2809 2982 3483 3981 5984
Size: 552 939 528 277 173 501 498 2003
Cuts at: 5984 513
Size: 3074
Fragments arranged by size:
3074 2003 939 552 528 501 498 277
173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 2240 3283 4743 6572 6589 6742
Size: 1255 656 4 1043 1460 1829 17 153
Cuts at: 6742 6927 7372 325
Size: 185 445 1498
Fragments arranged by size:
1829 1498 1460 1255 1043 656 445 185
153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2510 2666 3257
Size: 274 80 33 51 1443 533 156 591
Cuts at: 3257 3470 5018 6791 8503 96
Size: 213 1548 1773 1712 138
Fragments arranged by size:
1773 1712 1548 1443 591 533 274 213
156 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 8505 8537 2159
Size: 32 6314 32 2167
Fragments arranged by size:
6314 2167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8545
XbaI T'CTAG_A
Cuts at: 5365 5881 5365
Size: 516 8029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3337 4651 5443 3337
Size: 1314 792 6439
Fragments arranged by size:
6439 1314 792
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8545
XmaI C'CCGG_G
Cuts at: 1870 5870 1870
Size: 4000 4545
XmnI GAAnn'nnTTC
Cuts at: 2904 2955 3008 6674 2904
Size: 51 53 3666 4775
Fragments arranged by size:
4775 3666 53 51
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6353 276
Size: 53 83 186 936 4819 2468
Fragments arranged by size:
4819 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BsiWI BstZ17I
EcoRV FalI FseI FspAI NruI PacI PmlI PspOMI
PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI
SwaI