Restriction map for
pFN21AE4276
(Circular) (Six-base) MAPSORT of: pFN21AE4276.seq Check: 9789 from: 1 to: 8488
pFN21AE4276 8488 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4230 6298 278
Size: 53 83 186 936 2694 2068 2468
Fragments arranged by size:
2694 2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4108 5831 1937
Size: 2171 1723 4594
Fragments arranged by size:
4594 2171 1723
Acc65I G'GTAC_C
Cuts at: 5809 5809
Size: 8488
AclI AA'CG_TT
Cuts at: 1563 4765 6615 6988 1563
Size: 3202 1850 373 3063
Fragments arranged by size:
3202 3063 1850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3444 3462 3759 4797 5418 6551 7935 8203
Size: 1812 18 297 1038 621 1133 1384 268
Cuts at: 8203 1632
Size: 1917
Fragments arranged by size:
1917 1812 1384 1133 1038 621 297 268
18
AflII C'TTAA_G
Cuts at: 820 1017 2104 820
Size: 197 1087 7204
Fragments arranged by size:
7204 1087 197
AflIII A'CryG_T
Cuts at: 1203 2175 4318 7387 8364 1203
Size: 972 2143 3069 977 1327
Fragments arranged by size:
3069 2143 1327 977 972
AgeI A'CCGG_T
Cuts at: 7319 7319
Size: 8488
AhdI GACnn_n'nnGTC
Cuts at: 7217 7217
Size: 8488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8488
AlwNI CAG_nnn'CTG
Cuts at: 2788 3316 3782 6231 7803 2788
Size: 528 466 2449 1572 3473
Fragments arranged by size:
3473 2449 1572 528 466
ApaLI G'TGCA_C
Cuts at: 5304 6545 7701 5304
Size: 1241 1156 6091
Fragments arranged by size:
6091 1241 1156
ApoI r'AATT_y
Cuts at: 1493 2566 2760 2867 3231 3398 3415 3958 4019
Size: 1073 194 107 364 167 17 543 61
Cuts at: 4019 4116 4202 4289 4398 4444 4830 5131 5207
Size: 97 86 87 109 46 386 301 76
Cuts at: 5207 5241 5772 5797 6023 6175 8284 1493
Size: 34 531 25 226 152 2109 1697
Fragments arranged by size:
2109 1697 1073 543 531 386 364 301
226 194 167 152 109 107 97 87
86 76 61 46 34 25 17
AseI AT'TA_AT
Cuts at: 160 1032 7042 160
Size: 872 6010 1606
Fragments arranged by size:
6010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8488
AvaI C'yCGr_G
Cuts at: 1870 1943 2502 5813 1870
Size: 73 559 3311 4545
Fragments arranged by size:
4545 3311 559 73
BamHI G'GATC_C
Cuts at: 5818 6185 5818
Size: 367 8121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5809 7264 618
Size: 325 217 627 4022 1455 1842
Fragments arranged by size:
4022 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2097 5807 1391
Size: 706 3710 4072
Fragments arranged by size:
4072 3710 706
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3027 3060 4365 4448 5424 5685 5763
Size: 971 1128 33 1305 83 976 261 78
Cuts at: 5763 928
Size: 3653
Fragments arranged by size:
3653 1305 1128 976 971 261 83 78
33
BbvCI CC'TCA_GC
Cuts at: 5563 5563
Size: 8488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6679 6713 1903
Size: 34 4742 34 3678
Fragments arranged by size:
4742 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 6380 7590 4064
Size: 2316 1210 4962
Fragments arranged by size:
4962 2316 1210
BclI T'GATC_A
Cuts at: 1548 4711 5292 1548
Size: 3163 581 4744
Fragments arranged by size:
4744 3163 581
BfrBI ATG'CAT
Cuts at: 3000 4898 3000
Size: 1898 6590
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7099 136
Size: 107 122 71 631 605 5427 1525
Fragments arranged by size:
5427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3656 4656 8483 3656
Size: 1000 3827 3661
Fragments arranged by size:
3827 3661 1000
BlpI GC'TnA_GC
Cuts at: 5905 5905
Size: 8488
Bme1580I G_kGCm'C
Cuts at: 1790 5308 6549 7705 1790
Size: 3518 1241 1156 2573
Fragments arranged by size:
3518 2573 1241 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5295 5370 6219
Size: 416 53 83 399 24 3878 75 849
Cuts at: 6219 7177 442
Size: 958 1753
Fragments arranged by size:
3878 1753 958 849 416 399 83 75
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8488
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5049 5049
Size: 8488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 3657 5294 5321 7148 1239
Size: 1601 817 1637 27 1827 2579
Fragments arranged by size:
2579 1827 1637 1601 817 27
Bpu10I CC'TnA_GC
Cuts at: 4980 5563 4980
Size: 583 7905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 6613 7478 7776 2081
Size: 4532 865 298 2793
Fragments arranged by size:
4532 2793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5609 5719 7151 882
Size: 617 4110 110 1432 2219
Fragments arranged by size:
4110 2219 1432 617 110
BsaAI yAC'GTr
Cuts at: 493 1204 6279 493
Size: 711 5075 2702
Fragments arranged by size:
5075 2702 711
BsaBI GATnn'nnATC
Cuts at: 5592 5709 8482 5592
Size: 117 2773 5598
Fragments arranged by size:
5598 2773 117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4227 6295 6677 7312
Size: 53 83 186 936 2694 2068 382 635
Cuts at: 7312 275
Size: 1451
Fragments arranged by size:
2694 2068 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2128 3246 6921 7319 7593 7740 8151 2128
Size: 1118 3675 398 274 147 411 2465
Fragments arranged by size:
3675 2465 1118 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3050 3080 4561 4591 1178
Size: 30 165 30 1647 30 1481 30 5075
Fragments arranged by size:
5075 1647 1481 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3071 3146 4582 5558 5702 1186
Size: 1885 75 1436 976 144 3972
Fragments arranged by size:
3972 1885 1436 976 144 75
BseYI C'CCAG_C
Cuts at: 7691 8187 7691
Size: 496 7992
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2894 3069 3588 1163
Size: 573 1158 175 519 6063
Fragments arranged by size:
6063 1158 573 519 175
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5938 6699 7727 1540
Size: 275 186 3937 761 1028 2301
Fragments arranged by size:
3937 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3635 5308 5467 6549 6634 7705 3635
Size: 1673 159 1082 85 1071 4418
Fragments arranged by size:
4418 1673 1082 1071 159 85
BsmI GAATG_Cn'
Cuts at: 2977 4185 5748 5999 6092 2977
Size: 1208 1563 251 93 5373
Fragments arranged by size:
5373 1563 1208 251 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5419 5419
Size: 8488
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 3635 5308 5467 5807 6549 6634
Size: 399 307 1538 1673 159 340 742 85
Cuts at: 6634 7705 1391
Size: 1071 2174
Fragments arranged by size:
2174 1673 1538 1071 742 399 340 307
159 85
BspEI T'CCGG_A
Cuts at: 2128 3246 8151 2128
Size: 1118 4905 2465
Fragments arranged by size:
4905 2465 1118
BspHI T'CATG_A
Cuts at: 6377 8107 6377
Size: 1730 6758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3499 5843 844
Size: 2655 2344 3489
Fragments arranged by size:
3489 2655 2344
BsrBI CCG'CTC
Cuts at: 1394 1823 6375 1394
Size: 429 4552 3507
Fragments arranged by size:
4552 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2479 6983 7157 65
Size: 1183 1231 4504 174 1396
Fragments arranged by size:
4504 1396 1231 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7132 7319 1700
Size: 110 5322 187 2869
Fragments arranged by size:
5322 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4281 96
Size: 4185 4303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8488
BssSI C'ACGA_G
Cuts at: 5606 6548 7560 5606
Size: 942 1012 6534
Fragments arranged by size:
6534 1012 942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2972 3316 794
Size: 2178 344 5966
Fragments arranged by size:
5966 2178 344
BstBI TT'CG_AA
Cuts at: 7292 7292
Size: 8488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8488
BstXI CCAn_nnnn'nTGG
Cuts at: 4528 5345 4528
Size: 817 7671
BstYI r'GATC_y
Cuts at: 1972 3656 3860 4311 4656 5818 6185 6570 6587
Size: 1684 204 451 345 1162 367 385 17
Cuts at: 6587 8039 8125 8137 8483 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1684 1452 1162 451 385 367 346
345 204 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 5599 513
Size: 552 4534 3402
Fragments arranged by size:
4534 3402 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7749
Fragments arranged by size:
7749 683 56
BtsI GCAGTG_nn'
Cuts at: 2328 2777 5474 5577 6004 6798 6818 2328
Size: 449 2697 103 427 794 20 3998
Fragments arranged by size:
3998 2697 794 449 427 103 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5001 5036 529
Size: 35 4437 35 3981
Fragments arranged by size:
4437 3981 35 35
DraI TTT'AAA
Cuts at: 2218 2262 3462 5792 2218
Size: 44 1200 2330 4914
Fragments arranged by size:
4914 2330 1200 44
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7495 809
Size: 507 6179 1802
Fragments arranged by size:
6179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4370 5935 6824
Size: 54 1425 50 275 18 2540 1565 889
Cuts at: 6824 8453 8
Size: 1629 43
Fragments arranged by size:
2540 1629 1565 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5935 1537
Size: 275 4123 4090
Fragments arranged by size:
4123 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2393 2808 3048 3123 3305 3540 3915 6418
Size: 1040 415 240 75 182 235 375 2503
Cuts at: 6418 1353
Size: 3423
Fragments arranged by size:
3423 2503 1040 415 375 240 235 182
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3093 7075 7449 7595 387
Size: 1064 138 1504 3982 374 146 1280
Fragments arranged by size:
3982 1504 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2840 3444 3462 3657 3759 4797 5294
Size: 393 1208 604 18 195 102 1038 497
Cuts at: 5294 5321 5418 6551 7148 7935 8203 1239
Size: 27 97 1133 597 787 268 1524
Fragments arranged by size:
1524 1208 1133 1038 787 604 597 497
393 268 195 102 97 27 18
EcoO109I rG'GnC_Cy
Cuts at: 3581 4668 3581
Size: 1087 7401
EcoRI G'AATT_C
Cuts at: 2760 4019 4398 5797 6175 2760
Size: 1259 379 1399 378 5073
Fragments arranged by size:
5073 1399 1259 379 378
EcoRV GAT'ATC
Cuts at: 2158 4219 4804 2158
Size: 2061 585 5842
Fragments arranged by size:
5842 2061 585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 8488
FspI TGC'GCA
Cuts at: 6994 6994
Size: 8488
HaeII r_GCGC'y
Cuts at: 1678 7635 1678
Size: 5957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3050 3082 3542 3574 3656
Size: 32 135 32 1478 32 460 32 82
Cuts at: 3656 3688 4536 4568 4928 4960 5414 5446 5624
Size: 32 848 32 360 32 454 32 178
Cuts at: 5624 5656 7129 7161 7203 7235 1373
Size: 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1478 1473 848 460 454 360 178
135 82 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2060 3295 5010 5669 5832 6078
Size: 988 281 122 1235 1715 659 163 246
Cuts at: 6078 669
Size: 3079
Fragments arranged by size:
3079 1715 1235 988 659 281 246 163
122
HindIII A'AGCT_T
Cuts at: 748 2690 4164 748
Size: 1942 1474 5072
Fragments arranged by size:
5072 1942 1474
HpaI GTT'AAC
Cuts at: 3295 6078 3295
Size: 2783 5705
KpnI G_GTAC'C
Cuts at: 5813 5813
Size: 8488
MfeI C'AATT_G
Cuts at: 2844 4430 6087 2844
Size: 1586 1657 5245
Fragments arranged by size:
5245 1657 1586
MluI A'CGCG_T
Cuts at: 8364 8364
Size: 8488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 3105 4339 7602 7786 1305
Size: 831 969 1234 3263 184 2007
Fragments arranged by size:
3263 2007 1234 969 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4372 10
Size: 54 1425 343 2540 4126
Fragments arranged by size:
4126 2540 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3896 4323 6446 6805 6964 518
Size: 648 2730 427 2123 359 159 2042
Fragments arranged by size:
2730 2123 2042 648 427 359 159
MspA1I CmG'CkG
Cuts at: 1606 5904 6581 7729 7974 1606
Size: 4298 677 1148 245 2120
Fragments arranged by size:
4298 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7936
NdeI CA'TA_TG
Cuts at: 387 2635 5386 387
Size: 2248 2751 3489
Fragments arranged by size:
3489 2751 2248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8488
NotI GC'GGCC_GC
Cuts at: 5935 5935
Size: 8488
NsiI A_TGCA'T
Cuts at: 3002 4900 3002
Size: 1898 6590
NspI r_CATG'y
Cuts at: 1132 1229 2179 3004 3310 4322 4902 5516 5846
Size: 97 950 825 306 1012 580 614 330
Cuts at: 5846 7391 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1012 950 825 614 580 330
306 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8488
PciI A'CATG_T
Cuts at: 2175 4318 7387 2175
Size: 2143 3069 3276
Fragments arranged by size:
3276 3069 2143
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3722 1108
Size: 2614 5874
PfoI T'CCnGG_A
Cuts at: 2391 3682 3991 5310 2391
Size: 1291 309 1319 5569
Fragments arranged by size:
5569 1319 1291 309
PmeI GTTT'AAAC
Cuts at: 5792 5792
Size: 8488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2629 6557 6589 8070 8102 2597
Size: 32 3928 32 1481 32 2983
Fragments arranged by size:
3928 2983 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3581 4668 3581
Size: 1087 7401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8488
PsiI TTA'TAA
Cuts at: 3593 5505 6058 3593
Size: 1912 553 6023
Fragments arranged by size:
6023 1912 553
PstI C_TGCA'G
Cuts at: 830 2320 2784 3321 3742 5481 5840 830
Size: 1490 464 537 421 1739 359 3478
Fragments arranged by size:
3478 1739 1490 537 464 421 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8488
SalI G'TCGA_C
Cuts at: 1936 5830 1936
Size: 3894 4594
SanDI GG'GwC_CC
Cuts at: 4668 4668
Size: 8488
SapI GCTCTTCn'nnn_
Cuts at: 2808 2808
Size: 8488
SbfI CC_TGCA'GG
Cuts at: 5840 5840
Size: 8488
ScaI AGT'ACT
Cuts at: 6736 8448 6736
Size: 1712 6776
SfcI C'TryA_G
Cuts at: 826 1045 2316 2780 3317 3738 4109 5067 5234
Size: 219 1271 464 537 421 371 958 167
Cuts at: 5234 5477 5836 6971 7652 7843 826
Size: 243 359 1135 681 191 1471
Fragments arranged by size:
1471 1271 1135 958 681 537 464 421
371 359 243 219 191 167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8488
SmaI CCC'GGG
Cuts at: 1872 5815 1872
Size: 3943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 2104 2502 6592 7493 7755
Size: 197 926 153 8 398 4090 901 262
Cuts at: 7755 820
Size: 1553
Fragments arranged by size:
4090 1553 926 901 398 262 197 153
8
SnaBI TAC'GTA
Cuts at: 493 6279 493
Size: 5786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8488
SphI G_CATG'C
Cuts at: 1132 3004 5846 1132
Size: 1872 2842 3774
Fragments arranged by size:
3774 2842 1872
SspI AAT'ATT
Cuts at: 5 52 6412 5
Size: 47 6360 2081
Fragments arranged by size:
6360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8488
StyI C'CwwG_G
Cuts at: 513 1065 3796 3904 4562 5036 5729 5927 513
Size: 552 2731 108 658 474 693 198 3074
Fragments arranged by size:
3074 2731 693 658 552 474 198 108
SwaI ATTT'AAAT
Cuts at: 3462 3462
Size: 8488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5482 6515 6532 6685 6870 7315 325
Size: 1255 3902 1033 17 153 185 445 1498
Fragments arranged by size:
3902 1498 1255 1033 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3390 4281 4471
Size: 274 80 33 51 1443 1413 891 190
Cuts at: 4471 6734 8446 96
Size: 2263 1712 138
Fragments arranged by size:
2263 1712 1443 1413 891 274 190 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3243 3275 3604 3636 4561 4593 8448 8480 3243
Size: 32 329 32 925 32 3855 32 3251
Fragments arranged by size:
3855 3251 925 329 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4548 1316
Size: 3232 5256
XbaI T'CTAG_A
Cuts at: 2239 3211 3653 3748 4927 5824 2239
Size: 972 442 95 1179 897 4903
Fragments arranged by size:
4903 1179 972 897 442 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2719 2719
Size: 8488
XhoI C'TCGA_G
Cuts at: 1943 2502 1943
Size: 559 7929
XmaI C'CCGG_G
Cuts at: 1870 5813 1870
Size: 3943 4545
XmnI GAAnn'nnTTC
Cuts at: 2226 2569 4658 6617 2226
Size: 343 2089 1959 4097
Fragments arranged by size:
4097 2089 1959 343
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4228 6296 276
Size: 53 83 186 936 2694 2068 2468
Fragments arranged by size:
2694 2468 2068 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI
SfoI SrfI