Restriction map for
pFN21AE4273
(Circular) (Six-base) MAPSORT of: pFN21AE4273.seq Check: 5777 from: 1 to: 8272
pFN21AE4273 8272 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4316 4316
Size: 8272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6082 278
Size: 53 83 186 936 4546 2468
Fragments arranged by size:
4546 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2776 3243 3438 4960 5615 1937
Size: 839 467 195 1522 655 4594
Fragments arranged by size:
4594 1522 839 655 467 195
Acc65I G'GTAC_C
Cuts at: 3678 5593 3678
Size: 1915 6357
AclI AA'CG_TT
Cuts at: 1563 3012 6399 6772 1563
Size: 1449 3387 373 3063
Fragments arranged by size:
3387 3063 1449 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2844 3417 3557 3729 4548 4767 6335 7719
Size: 1212 573 140 172 819 219 1568 1384
Cuts at: 7719 7987 1632
Size: 268 1917
Fragments arranged by size:
1917 1568 1384 1212 819 573 268 219
172 140
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8075
AflIII A'CryG_T
Cuts at: 1203 3037 4378 7171 8148 1203
Size: 1834 1341 2793 977 1327
Fragments arranged by size:
2793 1834 1341 1327 977
AgeI A'CCGG_T
Cuts at: 7103 7103
Size: 8272
AhdI GACnn_n'nnGTC
Cuts at: 5358 7001 5358
Size: 1643 6629
AleI CACnn'nnGTG
Cuts at: 1166 5060 1166
Size: 3894 4378
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4276 1184
Size: 3092 5180
AlwNI CAG_nnn'CTG
Cuts at: 5057 6015 7587 5057
Size: 958 1572 5742
Fragments arranged by size:
5742 1572 958
ApaLI G'TGCA_C
Cuts at: 6329 7485 6329
Size: 1156 7116
ApoI r'AATT_y
Cuts at: 1493 2446 2638 2819 3087 3295 3855 4187 5233
Size: 953 192 181 268 208 560 332 1046
Cuts at: 5233 5513 5581 5807 5959 8068 1493
Size: 280 68 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 953 560 332 280 268
226 208 192 181 152 68
AseI AT'TA_AT
Cuts at: 160 1032 5183 5213 6826 160
Size: 872 4151 30 1613 1606
Fragments arranged by size:
4151 1613 1606 872 30
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8272
AvaI C'yCGr_G
Cuts at: 1870 1943 5597 1870
Size: 73 3654 4545
Fragments arranged by size:
4545 3654 73
BamHI G'GATC_C
Cuts at: 5602 5969 5602
Size: 367 7905
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3678 4485 5593 7048 618
Size: 325 217 627 1891 807 1108 1455 1842
Fragments arranged by size:
1891 1842 1455 1108 807 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2475 5591 1391
Size: 1084 3116 4072
Fragments arranged by size:
4072 3116 1084
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 3531 4805 928
Size: 971 354 1278 1274 4395
Fragments arranged by size:
4395 1278 1274 971 354
BbvCI CC'TCA_GC
Cuts at: 2540 2540
Size: 8272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4792 4826 6463 6497 1903
Size: 34 2855 34 1637 34 3678
Fragments arranged by size:
3678 2855 1637 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6164 7374 6164
Size: 1210 7062
BclI T'GATC_A
Cuts at: 1548 2980 3598 3670 1548
Size: 1432 618 72 6150
Fragments arranged by size:
6150 1432 618 72
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2741 6883 136
Size: 107 122 71 631 605 1069 4142 1525
Fragments arranged by size:
4142 1525 1069 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8267 8267
Size: 8272
BlpI GC'TnA_GC
Cuts at: 4788 5689 4788
Size: 901 7371
Bme1580I G_kGCm'C
Cuts at: 1790 6333 7489 1790
Size: 4543 1156 2573
Fragments arranged by size:
4543 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4063 1318
Size: 2745 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3345 4050 6003
Size: 416 53 83 399 24 1928 705 1953
Cuts at: 6003 6961 442
Size: 958 1753
Fragments arranged by size:
1953 1928 1753 958 705 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2565 4637 2565
Size: 2072 6200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3849 4203 6932 1239
Size: 2610 354 2729 2579
Fragments arranged by size:
2729 2610 2579 354
Bpu10I CC'TnA_GC
Cuts at: 2540 3584 2540
Size: 1044 7228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 2768 6397 7262 7560 2672
Size: 96 3629 865 298 3384
Fragments arranged by size:
3629 3384 865 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6935 882
Size: 617 5436 2219
Fragments arranged by size:
5436 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2845 6063 493
Size: 711 1641 3218 2702
Fragments arranged by size:
3218 2702 1641 711
BsaBI GATnn'nnATC
Cuts at: 3624 4677 8266 3624
Size: 1053 3589 3630
Fragments arranged by size:
3630 3589 1053
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3977 6079 6461 7096
Size: 53 83 186 936 2444 2102 382 635
Cuts at: 7096 275
Size: 1451
Fragments arranged by size:
2444 2102 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6705 7103 7377 7524 7935 6705
Size: 398 274 147 411 7042
Fragments arranged by size:
7042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 2558 2588 1178
Size: 30 165 30 874 30 251 30 6862
Fragments arranged by size:
6862 874 251 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2484 1186
Size: 1298 6974
BseYI C'CCAG_C
Cuts at: 3160 4562 7475 7971 3160
Size: 1402 2913 496 3461
Fragments arranged by size:
3461 2913 1402 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2589 2641 2867 3041 5052 5419 1163
Size: 573 853 52 226 174 2011 367 4016
Fragments arranged by size:
4016 2011 853 573 367 226 174 52
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5722 6483 7511 1540
Size: 275 186 3721 761 1028 2301
Fragments arranged by size:
3721 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2549 3655 5073 6333 6418 7489 2549
Size: 1106 1418 1260 85 1071 3332
Fragments arranged by size:
3332 1418 1260 1106 1071 85
BsmI GAATG_Cn'
Cuts at: 5783 5876 5783
Size: 93 8179
BsmBI CGTCTCn'nnnn_
Cuts at: 3968 4980 3968
Size: 1012 7260
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2475 2549 3655 5073 5591 6333 6418
Size: 399 685 74 1106 1418 518 742 85
Cuts at: 6418 7489 1391
Size: 1071 2174
Fragments arranged by size:
2174 1418 1106 1071 742 685 518 399
85 74
BspEI T'CCGG_A
Cuts at: 7935 7935
Size: 8272
BspHI T'CATG_A
Cuts at: 3882 6161 7891 3882
Size: 2279 1730 4263
Fragments arranged by size:
4263 2279 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4316 5627 844
Size: 3472 1311 3489
Fragments arranged by size:
3489 3472 1311
BsrBI CCG'CTC
Cuts at: 1394 1823 6159 1394
Size: 429 4336 3507
Fragments arranged by size:
4336 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4951 6767 6941 65
Size: 1183 3551 152 1816 174 1396
Fragments arranged by size:
3551 1816 1396 1183 174 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 6916 7103 1700
Size: 110 5106 187 2869
Fragments arranged by size:
5106 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3895 4759 96
Size: 3799 864 3609
Fragments arranged by size:
3799 3609 864
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8272
BssSI C'ACGA_G
Cuts at: 6332 7344 6332
Size: 1012 7260
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2619 4019 4997 794
Size: 1825 1400 978 4069
Fragments arranged by size:
4069 1825 1400 978
BstBI TT'CG_AA
Cuts at: 7076 7076
Size: 8272
BstEII G'GTnAC_C
Cuts at: 1184 2812 3265 1184
Size: 1628 453 6191
Fragments arranged by size:
6191 1628 453
BstXI CCAn_nnnn'nTGG
Cuts at: 2522 4116 2522
Size: 1594 6678
BstYI r'GATC_y
Cuts at: 1972 5602 5969 6354 6371 7823 7909 7921 8267
Size: 3630 367 385 17 1452 86 12 346
Cuts at: 8267 1972
Size: 1977
Fragments arranged by size:
3630 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2485 2485
Size: 8272
BtgI C'CryG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 5992
Fragments arranged by size:
5992 1728 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2501 487
Size: 683 56 1275 6258
Fragments arranged by size:
6258 1275 683 56
BtsI GCAGTG_nn'
Cuts at: 3187 4258 5788 6582 6602 3187
Size: 1071 1530 794 20 4857
Fragments arranged by size:
4857 1530 1071 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2664 2699 529
Size: 35 2100 35 6102
Fragments arranged by size:
6102 2100 35 35
DraI TTT'AAA
Cuts at: 4221 4461 4689 5400 5576 4221
Size: 240 228 711 176 6917
Fragments arranged by size:
6917 711 240 228 176
DraIII CAC_nnn'GTG
Cuts at: 2351 2351
Size: 8272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7279 809
Size: 507 5963 1802
Fragments arranged by size:
5963 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3553 5719 6608
Size: 54 1425 50 275 18 1723 2166 889
Cuts at: 6608 8237 8
Size: 1629 43
Fragments arranged by size:
2166 1723 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5719 1537
Size: 275 3907 4090
Fragments arranged by size:
4090 3907 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 2502 2587 3249 4786 5209 5486 6202
Size: 1127 22 85 662 1537 423 277 716
Cuts at: 6202 1353
Size: 3423
Fragments arranged by size:
3423 1537 1127 716 662 423 277 85
22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6859 7233 7379 387
Size: 1064 138 5270 374 146 1280
Fragments arranged by size:
5270 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2844 3417 3557 3729 3849 4203 4548
Size: 393 1212 573 140 172 120 354 345
Cuts at: 4548 4767 6335 6932 7719 7987 1239
Size: 219 1568 597 787 268 1524
Fragments arranged by size:
1568 1524 1212 787 597 573 393 354
345 268 219 172 140 120
EcoO109I rG'GnC_Cy
Cuts at: 2489 2489
Size: 8272
EcoRI G'AATT_C
Cuts at: 2446 2819 5581 5959 2446
Size: 373 2762 378 4759
Fragments arranged by size:
4759 2762 378 373
EcoRV GAT'ATC
Cuts at: 4446 4446
Size: 8272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4381 4381
Size: 8272
FspI TGC'GCA
Cuts at: 6778 6778
Size: 8272
HaeII r_GCGC'y
Cuts at: 1678 5147 7419 1678
Size: 3469 2272 2531
Fragments arranged by size:
3469 2531 2272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2645 2677 2692 2724 3760
Size: 32 135 32 1073 32 15 32 1036
Cuts at: 3760 3792 4162 4194 4490 4522 5344 5376 6913
Size: 32 370 32 296 32 822 32 1537
Cuts at: 6913 6945 6987 7019 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1537 1073 1036 822 370 296 135
42 32 32 32 32 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3244 3468 3539 4141 4920 4961
Size: 988 281 1306 224 71 602 779 41
Cuts at: 4961 5616 5862 669
Size: 655 246 3079
Fragments arranged by size:
3079 1306 988 779 655 602 281 246
224 71 41
HindIII A'AGCT_T
Cuts at: 748 2105 3560 748
Size: 1357 1455 5460
Fragments arranged by size:
5460 1455 1357
HpaI GTT'AAC
Cuts at: 3468 5862 3468
Size: 2394 5878
KpnI G_GTAC'C
Cuts at: 3682 5597 3682
Size: 1915 6357
MfeI C'AATT_G
Cuts at: 5871 5871
Size: 8272
MluI A'CGCG_T
Cuts at: 8148 8148
Size: 8272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3385 3706 3777 3915 7386 7570 1305
Size: 2080 321 71 138 3471 184 2007
Fragments arranged by size:
3471 2080 2007 321 184 138 71
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6450
Fragments arranged by size:
6450 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 3987 4114 5060 6230 6589 6748
Size: 648 1354 1467 127 946 1170 359 159
Cuts at: 6748 518
Size: 2042
Fragments arranged by size:
2042 1467 1354 1170 946 648 359 159
127
MspA1I CmG'CkG
Cuts at: 1606 4787 4925 5688 6365 7513 7758 1606
Size: 3181 138 763 677 1148 245 2120
Fragments arranged by size:
3181 2120 1148 763 677 245 138
NcoI C'CATG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 5992
Fragments arranged by size:
5992 1728 552
NdeI CA'TA_TG
Cuts at: 387 2911 387
Size: 2524 5748
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8272
NotI GC'GGCC_GC
Cuts at: 5719 5719
Size: 8272
NspI r_CATG'y
Cuts at: 1132 1229 2586 3041 4382 4918 4995 5630 7175
Size: 97 1357 455 1341 536 77 635 1545
Cuts at: 7175 1132
Size: 2229
Fragments arranged by size:
2229 1545 1357 1341 635 536 455 97
77
PasI CC'CwG_GG
Cuts at: 1348 2831 1348
Size: 1483 6789
PciI A'CATG_T
Cuts at: 3037 4378 7171 3037
Size: 1341 2793 4138
Fragments arranged by size:
4138 2793 1341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3635 4982 1108
Size: 2527 1347 4398
Fragments arranged by size:
4398 2527 1347
PfoI T'CCnGG_A
Cuts at: 2134 4180 2134
Size: 2046 6226
PmeI GTTT'AAAC
Cuts at: 5576 5576
Size: 8272
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 6341 6373 7854 7886 2873
Size: 32 3436 32 1481 32 3259
Fragments arranged by size:
3436 3259 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2489 2489
Size: 8272
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8272
PsiI TTA'TAA
Cuts at: 5842 5842
Size: 8272
PstI C_TGCA'G
Cuts at: 830 4429 5624 830
Size: 3599 1195 3478
Fragments arranged by size:
3599 3478 1195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8272
PvuII CAG'CTG
Cuts at: 4787 4925 4787
Size: 138 8134
SalI G'TCGA_C
Cuts at: 1936 3242 4959 5614 1936
Size: 1306 1717 655 4594
Fragments arranged by size:
4594 1717 1306 655
SapI GCTCTTCn'nnn_
Cuts at: 2480 5209 2480
Size: 2729 5543
SbfI CC_TGCA'GG
Cuts at: 5624 5624
Size: 8272
ScaI AGT'ACT
Cuts at: 3224 3593 6520 8232 3224
Size: 369 2927 1712 3264
Fragments arranged by size:
3264 2927 1712 369
SfcI C'TryA_G
Cuts at: 826 1045 3276 4425 4969 5620 6755 7436 7627
Size: 219 2231 1149 544 651 1135 681 191
Cuts at: 7627 826
Size: 1471
Fragments arranged by size:
2231 1471 1149 1135 681 651 544 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8272
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8272
SmaI CCC'GGG
Cuts at: 1872 5599 1872
Size: 3727 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 2783 6376 7277 7539 820
Size: 197 926 708 132 3593 901 262 1553
Fragments arranged by size:
3593 1553 926 901 708 262 197 132
SnaBI TAC'GTA
Cuts at: 493 6063 493
Size: 5570 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8272
SphI G_CATG'C
Cuts at: 1132 4995 5630 1132
Size: 3863 635 3774
Fragments arranged by size:
3863 3774 635
SspI AAT'ATT
Cuts at: 5 52 2231 2386 6196 5
Size: 47 2179 155 3810 2081
Fragments arranged by size:
3810 2179 2081 155 47
StuI AGG'CCT
Cuts at: 1517 2626 1517
Size: 1109 7163
StyI C'CwwG_G
Cuts at: 513 1065 2793 4083 5711 513
Size: 552 1728 1290 1628 3074
Fragments arranged by size:
3074 1728 1628 1290 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2386 2490 2844 4076 6299 6316 6469
Size: 1255 806 104 354 1232 2223 17 153
Cuts at: 6469 6654 7099 325
Size: 185 445 1498
Fragments arranged by size:
2223 1498 1255 1232 806 445 354 185
153 104 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 3222 3591
Size: 274 80 33 51 1443 993 252 369
Cuts at: 3591 3718 3826 3895 4387 4759 6518 8230 96
Size: 127 108 69 492 372 1759 1712 138
Fragments arranged by size:
1759 1712 1443 993 492 372 369 274
252 138 127 108 80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2354 3659 3691 4627 4659 4895 4927 8232
Size: 32 1305 32 936 32 236 32 3305
Cuts at: 8232 8264 2322
Size: 32 2330
Fragments arranged by size:
3305 2330 1305 936 236 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3774 1316
Size: 2458 5814
XbaI T'CTAG_A
Cuts at: 4508 5608 4508
Size: 1100 7172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 4081 2230
Size: 1851 6421
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8272
XmaI C'CCGG_G
Cuts at: 1870 5597 1870
Size: 3727 4545
XmnI GAAnn'nnTTC
Cuts at: 3072 5203 5472 6401 3072
Size: 2131 269 929 4943
Fragments arranged by size:
4943 2131 929 269
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6080 276
Size: 53 83 186 936 4546 2468
Fragments arranged by size:
4546 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI