Restriction map for
pFN21AE4269
(Circular) (Six-base) MAPSORT of: pFN21AE4269.seq Check: 8989 from: 1 to: 8092
pFN21AE4269 8092 bp
With 182 enzymes: SgfI *
March 20, 2014 17:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3128 3128
Size: 8092
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5902 278
Size: 53 83 186 936 4366 2468
Fragments arranged by size:
4366 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5435 1937
Size: 3498 4594
Acc65I G'GTAC_C
Cuts at: 2991 5413 2991
Size: 2422 5670
AclI AA'CG_TT
Cuts at: 1563 6219 6592 1563
Size: 4656 373 3063
Fragments arranged by size:
4656 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1998 2201 5081 5148 6155 7539 7807 1632
Size: 366 203 2880 67 1007 1384 268 1917
Fragments arranged by size:
2880 1917 1384 1007 366 268 203 67
AfeI AGC'GCT
Cuts at: 4199 4199
Size: 8092
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7895
AflIII A'CryG_T
Cuts at: 1203 4102 6991 7968 1203
Size: 2899 2889 977 1327
Fragments arranged by size:
2899 2889 1327 977
AgeI A'CCGG_T
Cuts at: 6923 6923
Size: 8092
AhdI GACnn_n'nnGTC
Cuts at: 6821 6821
Size: 8092
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8092
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3028 3060 3658 3690 3906 3938 3028
Size: 32 598 32 216 32 7182
Fragments arranged by size:
7182 598 216 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2606 5835 7407 2606
Size: 3229 1572 3291
Fragments arranged by size:
3291 3229 1572
ApaI G_GGCC'C
Cuts at: 4551 4551
Size: 8092
ApaLI G'TGCA_C
Cuts at: 6149 7305 6149
Size: 1156 6936
ApoI r'AATT_y
Cuts at: 1493 3568 5401 5627 5779 7888 1493
Size: 2075 1833 226 152 2109 1697
Fragments arranged by size:
2109 2075 1833 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6646 160
Size: 872 5614 1606
Fragments arranged by size:
5614 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8092
AvaI C'yCGr_G
Cuts at: 1870 1943 5417 1870
Size: 73 3474 4545
Fragments arranged by size:
4545 3474 73
AvrII C'CTAG_G
Cuts at: 4388 4388
Size: 8092
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5154 5187 5154
Size: 33 8059
BamHI G'GATC_C
Cuts at: 3015 5422 5789 3015
Size: 2407 367 5318
Fragments arranged by size:
5318 2407 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2348 2637 2991 5413 6868
Size: 325 217 627 561 289 354 2422 1455
Cuts at: 6868 618
Size: 1842
Fragments arranged by size:
2422 1842 1455 627 561 354 325 289
217
BanII G_rGCy'C
Cuts at: 1391 4551 5262 5411 1391
Size: 3160 711 149 4072
Fragments arranged by size:
4072 3160 711 149
BbeI G_GCGC'C
Cuts at: 2641 2641
Size: 8092
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3150 3315 3674 5136 5228 928
Size: 971 1251 165 359 1462 92 3792
Fragments arranged by size:
3792 1462 1251 971 359 165 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6283 6317 1903
Size: 34 4346 34 3678
Fragments arranged by size:
4346 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2930 5984 7194 2930
Size: 3054 1210 3828
Fragments arranged by size:
3828 3054 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8092
BfrBI ATG'CAT
Cuts at: 2947 2947
Size: 8092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6703 136
Size: 107 122 71 631 605 5031 1525
Fragments arranged by size:
5031 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2009 2076 2485 4063 4323 8087 2009
Size: 67 409 1578 260 3764 2014
Fragments arranged by size:
3764 2014 1578 409 260 67
BlpI GC'TnA_GC
Cuts at: 5037 5509 5037
Size: 472 7620
Bme1580I G_kGCm'C
Cuts at: 1790 4551 6153 7309 1790
Size: 2761 1602 1156 2573
Fragments arranged by size:
2761 2573 1602 1156
BmgBI CAC'GTC
Cuts at: 1318 2133 4105 1318
Size: 815 1972 5305
Fragments arranged by size:
5305 1972 815
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4048 5823 6781
Size: 416 53 83 399 24 2631 1775 958
Cuts at: 6781 442
Size: 1753
Fragments arranged by size:
2631 1775 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5003 1056
Size: 3947 4145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4503 4503
Size: 8092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 2014 2204 2396 2939 4032 4216 4833
Size: 766 9 190 192 543 1093 184 617
Cuts at: 4833 5037 6752 1239
Size: 204 1715 2579
Fragments arranged by size:
2579 1715 1093 766 617 543 204 192
190 184 9
Bpu10I CC'TnA_GC
Cuts at: 3974 4875 3974
Size: 901 7191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 3728 6217 7082 7380 2474
Size: 1254 2489 865 298 3186
Fragments arranged by size:
3186 2489 1254 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2096 2457 4809 4986 6755 882
Size: 617 597 361 2352 177 1769 2219
Fragments arranged by size:
2352 2219 1769 617 597 361 177
BsaAI yAC'GTr
Cuts at: 493 1204 4111 5883 493
Size: 711 2907 1772 2702
Fragments arranged by size:
2907 2702 1772 711
BsaBI GATnn'nnATC
Cuts at: 4161 8086 4161
Size: 3925 4167
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2638 5106 5165 5899
Size: 53 83 186 936 1105 2468 59 734
Cuts at: 5899 6281 6916 275
Size: 382 635 1451
Fragments arranged by size:
2468 1451 1105 936 734 635 382 186
83 59 53
BsaWI w'CCGG_w
Cuts at: 3018 6525 6923 7197 7344 7755 3018
Size: 3507 398 274 147 411 3355
Fragments arranged by size:
3507 3355 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3028 3058 4357 4387 1178
Size: 30 165 30 1625 30 1299 30 4883
Fragments arranged by size:
4883 1625 1299 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 3049 4154 4219 4476 1186
Size: 1015 848 1105 65 257 4802
Fragments arranged by size:
4802 1105 1015 848 257 65
BseYI C'CCAG_C
Cuts at: 3575 4529 4635 4906 4987 7295 7791 3575
Size: 954 106 271 81 2308 496 3876
Fragments arranged by size:
3876 2308 954 496 271 106 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2850 5114 1163
Size: 573 402 712 2264 4141
Fragments arranged by size:
4141 2264 712 573 402
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5542 6303 7331 1540
Size: 275 186 3541 761 1028 2301
Fragments arranged by size:
3541 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2565 4649 5262 6153 6238 7309 2565
Size: 2084 613 891 85 1071 3348
Fragments arranged by size:
3348 2084 1071 891 613 85
BsmI GAATG_Cn'
Cuts at: 3474 5603 5696 3474
Size: 2129 93 5870
Fragments arranged by size:
5870 2129 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5097 5156 5097
Size: 59 8033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2565 4551 4649 5262 5411 6153 6238
Size: 399 775 1986 98 613 149 742 85
Cuts at: 6238 7309 1391
Size: 1071 2174
Fragments arranged by size:
2174 1986 1071 775 742 613 399 149
98 85
BspEI T'CCGG_A
Cuts at: 3018 7755 3018
Size: 4737 3355
BspHI T'CATG_A
Cuts at: 5981 7711 5981
Size: 1730 6362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3003 3128 4827 4923 4977 5004 5447 844
Size: 2159 125 1699 96 54 27 443 3489
Fragments arranged by size:
3489 2159 1699 443 125 96 54 27
BsrBI CCG'CTC
Cuts at: 1394 1823 5979 1394
Size: 429 4156 3507
Fragments arranged by size:
4156 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6587 6761 65
Size: 1183 5339 174 1396
Fragments arranged by size:
5339 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2925 6736 6923 1700
Size: 110 1115 3811 187 2869
Fragments arranged by size:
3811 2869 1115 187 110
BsrGI T'GTAC_A
Cuts at: 96 2269 3874 3942 96
Size: 2173 1605 68 4246
Fragments arranged by size:
4246 2173 1605 68
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8092
BssSI C'ACGA_G
Cuts at: 6152 7164 6152
Size: 1012 7080
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4664 794
Size: 3870 4222
BstBI TT'CG_AA
Cuts at: 6896 6896
Size: 8092
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8092
BstXI CCAn_nnnn'nTGG
Cuts at: 4840 4840
Size: 8092
BstYI r'GATC_y
Cuts at: 1972 2009 2076 2485 2707 3015 4063 4323 5077
Size: 37 67 409 222 308 1048 260 754
Cuts at: 5077 5422 5789 6174 6191 7643 7729 7741 8087
Size: 345 367 385 17 1452 86 12 346
Cuts at: 8087 1972
Size: 1977
Fragments arranged by size:
1977 1452 1048 754 409 385 367 346
345 308 260 222 86 67 37 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3264 3274 4365 513
Size: 552 2199 10 1091 4240
Fragments arranged by size:
4240 2199 1091 552 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2080 4170 487
Size: 683 56 854 2090 4409
Fragments arranged by size:
4409 2090 854 683 56
BtsI GCAGTG_nn'
Cuts at: 2393 2983 3633 3779 4259 5174 5608 6402 6422
Size: 590 650 146 480 915 434 794 20
Cuts at: 6422 2393
Size: 4063
Fragments arranged by size:
4063 915 794 650 590 480 434 146
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8092
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8057
DraI TTT'AAA
Cuts at: 5396 5396
Size: 8092
DraIII CAC_nnn'GTG
Cuts at: 2431 2431
Size: 8092
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7099 809
Size: 507 5783 1802
Fragments arranged by size:
5783 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3522 5323 5539
Size: 54 1425 50 275 18 1692 1801 216
Cuts at: 5539 6428 8057 8
Size: 889 1629 43
Fragments arranged by size:
1801 1692 1629 1425 889 275 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5539 1537
Size: 275 3727 4090
Fragments arranged by size:
4090 3727 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2898 3246 4472 4603 5295 6022 1353
Size: 1545 348 1226 131 692 727 3423
Fragments arranged by size:
3423 1545 1226 727 692 348 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6679 7053 7199 387
Size: 1064 138 5090 374 146 1280
Fragments arranged by size:
5090 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5260 5260
Size: 8092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2005 2014 2201 2204 2396 2939
Size: 393 366 7 9 187 3 192 543
Cuts at: 2939 4032 4216 4833 5037 5081 5148 6155 6752
Size: 1093 184 617 204 44 67 1007 597
Cuts at: 6752 7539 7807 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1093 1007 787 617 597 543 393
366 268 204 192 187 184 67 44
9 7 3
EcoNI CCTnn'n_nnAGG
Cuts at: 4878 4878
Size: 8092
EcoO109I rG'GnC_Cy
Cuts at: 3256 4548 4912 4966 3256
Size: 1292 364 54 6382
Fragments arranged by size:
6382 1292 364 54
EcoRI G'AATT_C
Cuts at: 5401 5779 5401
Size: 378 7714
EcoRV GAT'ATC
Cuts at: 3168 3228 3168
Size: 60 8032
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4085 5374 4085
Size: 1289 6803
FspI TGC'GCA
Cuts at: 6598 6598
Size: 8092
HaeII r_GCGC'y
Cuts at: 1678 2641 4201 7239 1678
Size: 963 1560 3038 2531
Fragments arranged by size:
3038 2531 1560 963
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2105 2137 3137
Size: 32 135 32 491 32 10 32 1000
Cuts at: 3137 3152 3169 3184 3362 3394 3833 3865 4616
Size: 15 17 15 178 32 439 32 751
Cuts at: 4616 4648 4715 4747 5094 5126 6733 6765 6807
Size: 32 67 32 347 32 1607 32 42
Cuts at: 6807 6839 1373
Size: 32 2626
Fragments arranged by size:
2626 1607 1000 751 491 439 347 178
135 67 42 32 32 32 32 32
32 32 32 32 32 32 17 15
15 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3865 4003 5436 5682 669
Size: 988 281 1927 138 1433 246 3079
Fragments arranged by size:
3079 1927 1433 988 281 246 138
HindIII A'AGCT_T
Cuts at: 748 3965 748
Size: 3217 4875
HpaI GTT'AAC
Cuts at: 4003 5682 4003
Size: 1679 6413
KasI G'GCGC_C
Cuts at: 2637 2637
Size: 8092
KpnI G_GTAC'C
Cuts at: 2995 5417 2995
Size: 2422 5670
MfeI C'AATT_G
Cuts at: 5691 5691
Size: 8092
MluI A'CGCG_T
Cuts at: 7968 7968
Size: 8092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4433 4545 5160 7206 7390 1305
Size: 3128 112 615 2046 184 2007
Fragments arranged by size:
3128 2046 2007 615 184 112
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3524 5325 10
Size: 54 1425 343 1692 1801 2777
Fragments arranged by size:
2777 1801 1692 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6050 6409 6568 518
Size: 648 4884 359 159 2042
Fragments arranged by size:
4884 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2777 3343 4294 4906 4987 5508 6185 7333
Size: 1171 566 951 612 81 521 677 1148
Cuts at: 7333 7578 1606
Size: 245 2120
Fragments arranged by size:
2120 1171 1148 951 677 612 566 521
245 81
NaeI GCC'GGC
Cuts at: 2927 2927
Size: 8092
NarI GG'CG_CC
Cuts at: 2638 2638
Size: 8092
NcoI C'CATG_G
Cuts at: 513 1065 3264 513
Size: 552 2199 5341
Fragments arranged by size:
5341 2199 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8092
NgoMIV G'CCGG_C
Cuts at: 2925 2925
Size: 8092
NheI G'CTAG_C
Cuts at: 1052 4999 1052
Size: 3947 4145
NotI GC'GGCC_GC
Cuts at: 5539 5539
Size: 8092
NsiI A_TGCA'T
Cuts at: 2949 2949
Size: 8092
NspI r_CATG'y
Cuts at: 1132 1229 2947 3080 5450 6995 1132
Size: 97 1718 133 2370 1545 2229
Fragments arranged by size:
2370 2229 1718 1545 133 97
PasI CC'CwG_GG
Cuts at: 1348 3350 3910 1348
Size: 2002 560 5530
Fragments arranged by size:
5530 2002 560
PciI A'CATG_T
Cuts at: 6991 6991
Size: 8092
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4811 1108
Size: 3703 4389
PmeI GTTT'AAAC
Cuts at: 5396 5396
Size: 8092
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3060 4636 4668 6161 6193 7674 7706 3028
Size: 32 1576 32 1493 32 1481 32 3414
Fragments arranged by size:
3414 1576 1493 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4912 4966 4912
Size: 54 8038
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8092
PsiI TTA'TAA
Cuts at: 2726 5662 2726
Size: 2936 5156
PspOMI G'GGCC_C
Cuts at: 4547 4547
Size: 8092
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3418 3450 3418
Size: 32 8060
PstI C_TGCA'G
Cuts at: 830 2165 2718 3841 5444 830
Size: 1335 553 1123 1603 3478
Fragments arranged by size:
3478 1603 1335 1123 553
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8092
PvuII CAG'CTG
Cuts at: 2777 4906 4987 2777
Size: 2129 81 5882
Fragments arranged by size:
5882 2129 81
SacI G_AGCT'C
Cuts at: 5262 5262
Size: 8092
SalI G'TCGA_C
Cuts at: 1936 5434 1936
Size: 3498 4594
SbfI CC_TGCA'GG
Cuts at: 5444 5444
Size: 8092
ScaI AGT'ACT
Cuts at: 6340 8052 6340
Size: 1712 6380
SfcI C'TryA_G
Cuts at: 826 1045 2161 2714 3837 3934 5440 6575 7256
Size: 219 1116 553 1123 97 1506 1135 681
Cuts at: 7256 7447 826
Size: 191 1471
Fragments arranged by size:
1506 1471 1135 1123 1116 681 553 219
191 97
SfoI GGC'GCC
Cuts at: 2639 2639
Size: 8092
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8092
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8092
SmaI CCC'GGG
Cuts at: 1872 5419 1872
Size: 3547 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 3707 6196 7097 7359 820
Size: 197 926 546 1218 2489 901 262 1553
Fragments arranged by size:
2489 1553 1218 926 901 546 262 197
SnaBI TAC'GTA
Cuts at: 493 5883 493
Size: 5390 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8092
SphI G_CATG'C
Cuts at: 1132 2947 5450 1132
Size: 1815 2503 3774
Fragments arranged by size:
3774 2503 1815
SspI AAT'ATT
Cuts at: 5 52 6016 5
Size: 47 5964 2081
Fragments arranged by size:
5964 2081 47
StuI AGG'CCT
Cuts at: 1517 4483 1517
Size: 2966 5126
StyI C'CwwG_G
Cuts at: 513 1065 2344 2442 3264 4280 4388 4571 4669
Size: 552 1279 98 822 1016 108 183 98
Cuts at: 4669 5031 5531 513
Size: 362 500 3074
Fragments arranged by size:
3074 1279 1016 822 552 500 362 183
108 98 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4219 4457 4936 5044 5085 6119 6136
Size: 1255 2639 238 479 108 41 1034 17
Cuts at: 6136 6289 6474 6919 325
Size: 153 185 445 1498
Fragments arranged by size:
2639 1498 1255 1034 479 445 238 185
153 108 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2269 2793 3874
Size: 274 80 33 51 1443 292 524 1081
Cuts at: 3874 3942 6338 8050 96
Size: 68 2396 1712 138
Fragments arranged by size:
2396 1712 1443 1081 524 292 274 138
80 68 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4357 4389 5297 5329 8052 8084 4357
Size: 32 908 32 2723 32 4365
Fragments arranged by size:
4365 2723 908 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8092
XbaI T'CTAG_A
Cuts at: 2194 5428 2194
Size: 3234 4858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2701 4082 4451 4903 4984 2701
Size: 1381 369 452 81 5809
Fragments arranged by size:
5809 1381 452 369 81
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8092
XmaI C'CCGG_G
Cuts at: 1870 5417 1870
Size: 3547 4545
XmnI GAAnn'nnTTC
Cuts at: 4764 6221 4764
Size: 1457 6635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5900 276
Size: 53 83 186 936 4366 2468
Fragments arranged by size:
4366 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbvCI BsiWI BstZ17I Bsu36I FseI FspAI NruI
PacI PfoI PmlI PspXI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI