Restriction map for
pFN21AE4264
(Circular) (Six-base) MAPSORT of: pFN21AE4264.seq Check: 1796 from: 1 to: 7912
pFN21AE4264 7912 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5722 278
Size: 53 83 186 936 4186 2468
Fragments arranged by size:
4186 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4537 5255 1937
Size: 2600 718 4594
Fragments arranged by size:
4594 2600 718
Acc65I G'GTAC_C
Cuts at: 5233 5233
Size: 7912
AclI AA'CG_TT
Cuts at: 1563 6039 6412 1563
Size: 4476 373 3063
Fragments arranged by size:
4476 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2028 2415 2601 2716 3316 3465 3729 4014
Size: 396 387 186 115 600 149 264 285
Cuts at: 4014 5975 7359 7627 1632
Size: 1961 1384 268 1917
Fragments arranged by size:
1961 1917 1384 600 396 387 285 268
264 186 149 115
AflII C'TTAA_G
Cuts at: 820 1017 2506 2524 820
Size: 197 1489 18 6208
Fragments arranged by size:
6208 1489 197 18
AflIII A'CryG_T
Cuts at: 1203 2740 4167 6811 7788 1203
Size: 1537 1427 2644 977 1327
Fragments arranged by size:
2644 1537 1427 1327 977
AgeI A'CCGG_T
Cuts at: 6743 6743
Size: 7912
AhdI GACnn_n'nnGTC
Cuts at: 6641 6641
Size: 7912
AleI CACnn'nnGTG
Cuts at: 1166 2417 1166
Size: 1251 6661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7912
AlwNI CAG_nnn'CTG
Cuts at: 2182 2840 4778 5655 7227 2182
Size: 658 1938 877 1572 2867
Fragments arranged by size:
2867 1938 1572 877 658
ApaLI G'TGCA_C
Cuts at: 5969 7125 5969
Size: 1156 6756
ApoI r'AATT_y
Cuts at: 1493 3355 3612 4085 4208 4887 4925 5221 5447
Size: 1862 257 473 123 679 38 296 226
Cuts at: 5447 5599 7708 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1862 1697 679 473 296 257 226
152 123 38
AseI AT'TA_AT
Cuts at: 160 1032 6466 160
Size: 872 5434 1606
Fragments arranged by size:
5434 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7912
AvaI C'yCGr_G
Cuts at: 1870 1943 3825 5237 1870
Size: 73 1882 1412 4545
Fragments arranged by size:
4545 1882 1412 73
BamHI G'GATC_C
Cuts at: 2212 5242 5609 2212
Size: 3030 367 4515
Fragments arranged by size:
4515 3030 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4337 4917 5233 6688 618
Size: 325 217 627 2550 580 316 1455 1842
Fragments arranged by size:
2550 1842 1455 627 580 325 316 217
BanII G_rGCy'C
Cuts at: 1391 2346 2730 4044 5171 5231 1391
Size: 955 384 1314 1127 60 4072
Fragments arranged by size:
4072 1314 1127 955 384 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2737 3041 4219 4506 5150 928
Size: 971 838 304 1178 287 644 3690
Fragments arranged by size:
3690 1178 971 838 644 304 287
BbvCI CC'TCA_GC
Cuts at: 4635 4635
Size: 7912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6103 6137 1903
Size: 34 4166 34 3678
Fragments arranged by size:
4166 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2772 3601 5804 7014 2772
Size: 829 2203 1210 3670
Fragments arranged by size:
3670 2203 1210 829
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6523 136
Size: 107 122 71 631 605 4851 1525
Fragments arranged by size:
4851 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7907 7907
Size: 7912
BlpI GC'TnA_GC
Cuts at: 5329 5329
Size: 7912
Bme1580I G_kGCm'C
Cuts at: 1790 5973 7129 1790
Size: 4183 1156 2573
Fragments arranged by size:
4183 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2743 5148 1318
Size: 1425 2405 4082
Fragments arranged by size:
4082 2405 1425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4174 5643 6601
Size: 416 53 83 399 24 2757 1469 958
Cuts at: 6601 442
Size: 1753
Fragments arranged by size:
2757 1753 1469 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2198 3743 1056
Size: 1142 1545 5225
Fragments arranged by size:
5225 1545 1142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3302 3516 5045 6572 1239
Size: 2063 214 1529 1527 2579
Fragments arranged by size:
2579 2063 1529 1527 214
Bpu10I CC'TnA_GC
Cuts at: 4043 4635 4043
Size: 592 7320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 4997 6037 6902 7200 2135
Size: 2862 1040 865 298 2847
Fragments arranged by size:
2862 2847 1040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3661 6575 882
Size: 617 2162 2914 2219
Fragments arranged by size:
2914 2219 2162 617
BsaAI yAC'GTr
Cuts at: 493 1204 5703 493
Size: 711 4499 2702
Fragments arranged by size:
4499 2702 711
BsaBI GATnn'nnATC
Cuts at: 2519 2993 7906 2519
Size: 474 4913 2525
Fragments arranged by size:
4913 2525 474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4534 5719 6101 6736
Size: 53 83 186 936 3001 1185 382 635
Cuts at: 6736 275
Size: 1451
Fragments arranged by size:
3001 1451 1185 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2748 6345 6743 7017 7164 7575 2748
Size: 3597 398 274 147 411 3085
Fragments arranged by size:
3597 3085 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5063 5093 1178
Size: 30 165 30 3660 30 3997
Fragments arranged by size:
3997 3660 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3472 3519 3735 3891 1186
Size: 2286 47 216 156 5207
Fragments arranged by size:
5207 2286 216 156 47
BseYI C'CCAG_C
Cuts at: 3847 4491 7115 7611 3847
Size: 644 2624 496 4148
Fragments arranged by size:
4148 2624 644 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2829 2949 3023 3624 5150 1163
Size: 573 1093 120 74 601 1526 3925
Fragments arranged by size:
3925 1526 1093 601 573 120 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5362 6123 7151 1540
Size: 275 186 3361 761 1028 2301
Fragments arranged by size:
3361 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2730 3978 5171 5973 6058 7129 2730
Size: 1248 1193 802 85 1071 3513
Fragments arranged by size:
3513 1248 1193 1071 802 85
BsmI GAATG_Cn'
Cuts at: 3783 4004 5423 5516 3783
Size: 221 1419 93 6179
Fragments arranged by size:
6179 1419 221 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5000 5000
Size: 7912
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2346 2730 3978 4044 5171 5231 5973
Size: 399 556 384 1248 66 1127 60 742
Cuts at: 5973 6058 7129 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1248 1127 1071 742 556 399 384
85 66 60
BspEI T'CCGG_A
Cuts at: 7575 7575
Size: 7912
BspHI T'CATG_A
Cuts at: 5801 7531 5801
Size: 1730 6182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4251 4323 5267 844
Size: 3407 72 944 3489
Fragments arranged by size:
3489 3407 944 72
BsrBI CCG'CTC
Cuts at: 1394 1823 4380 4522 4915 5799 1394
Size: 429 2557 142 393 884 3507
Fragments arranged by size:
3507 2557 884 429 393 142
BsrDI GCAATG_nn'
Cuts at: 65 1248 4078 6407 6581 65
Size: 1183 2830 2329 174 1396
Fragments arranged by size:
2830 2329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6556 6743 1700
Size: 110 4746 187 2869
Fragments arranged by size:
4746 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4131 96
Size: 4035 3877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7912
BssSI C'ACGA_G
Cuts at: 5972 6984 5972
Size: 1012 6900
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7912
BstBI TT'CG_AA
Cuts at: 4068 4304 6716 4068
Size: 236 2412 5264
Fragments arranged by size:
5264 2412 236
BstEII G'GTnAC_C
Cuts at: 1184 4594 1184
Size: 3410 4502
BstXI CCAn_nnnn'nTGG
Cuts at: 4873 4873
Size: 7912
BstYI r'GATC_y
Cuts at: 1972 2212 2602 3578 5242 5609 5994 6011 7463
Size: 240 390 976 1664 367 385 17 1452
Cuts at: 7463 7549 7561 7907 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1664 1452 976 390 385 367 346
240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6421
Fragments arranged by size:
6421 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7173
Fragments arranged by size:
7173 683 56
BtsI GCAGTG_nn'
Cuts at: 2796 2838 4200 5428 6222 6242 2796
Size: 42 1362 1228 794 20 4466
Fragments arranged by size:
4466 1362 1228 794 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7912
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7877
DraI TTT'AAA
Cuts at: 2962 5216 2962
Size: 2254 5658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6919 809
Size: 507 5603 1802
Fragments arranged by size:
5603 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2421 5359 6248
Size: 54 1425 50 275 18 591 2938 889
Cuts at: 6248 7877 8
Size: 1629 43
Fragments arranged by size:
2938 1629 1425 889 591 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5359 1537
Size: 275 3547 4090
Fragments arranged by size:
4090 3547 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2047 2657 2735 3540 3720 3927 4698 4719
Size: 694 610 78 805 180 207 771 21
Cuts at: 4719 4992 5842 1353
Size: 273 850 3423
Fragments arranged by size:
3423 850 805 771 694 610 273 207
180 78 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2795 6499 6873 7019 387
Size: 1064 138 1206 3704 374 146 1280
Fragments arranged by size:
3704 1280 1206 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2728 5169 2728
Size: 2441 5471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2415 2601 2716 3302 3316 3465
Size: 393 396 387 186 115 586 14 149
Cuts at: 3465 3516 3729 4014 5045 5975 6572 7359 7627
Size: 51 213 285 1031 930 597 787 268
Cuts at: 7627 1239
Size: 1524
Fragments arranged by size:
1524 1031 930 787 597 586 396 393
387 285 268 213 186 149 115 51
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2382 4109 5123 2382
Size: 1727 1014 5171
Fragments arranged by size:
5171 1727 1014
EcoO109I rG'GnC_Cy
Cuts at: 4621 4621
Size: 7912
EcoRI G'AATT_C
Cuts at: 5221 5599 5221
Size: 378 7534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4081 4081
Size: 7912
FspI TGC'GCA
Cuts at: 6418 6418
Size: 7912
HaeII r_GCGC'y
Cuts at: 1678 4105 7059 1678
Size: 2427 2954 2531
Fragments arranged by size:
2954 2531 2427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2096 2128 2200
Size: 32 135 32 467 32 25 32 72
Cuts at: 2200 2232 3092 3119 3124 3145 3151 3177 3511
Size: 32 860 27 5 21 6 26 334
Cuts at: 3511 3543 3711 3743 4813 4845 6553 6585 6627
Size: 32 168 32 1070 32 1708 32 42
Cuts at: 6627 6659 1373
Size: 32 2626
Fragments arranged by size:
2626 1708 1070 860 467 334 168 135
72 42 32 32 32 32 32 32
32 32 32 32 27 26 25 21
6 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4323 5256 5502 669
Size: 988 281 2385 933 246 3079
Fragments arranged by size:
3079 2385 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 2397 748
Size: 1649 6263
HpaI GTT'AAC
Cuts at: 5502 5502
Size: 7912
KpnI G_GTAC'C
Cuts at: 5237 5237
Size: 7912
MfeI C'AATT_G
Cuts at: 5511 5511
Size: 7912
MluI A'CGCG_T
Cuts at: 7788 7788
Size: 7912
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2005 4077 4539 7026 7210 1305
Size: 700 2072 462 2487 184 2007
Fragments arranged by size:
2487 2072 2007 700 462 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6090
Fragments arranged by size:
6090 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2718 2999 3756 4170 5870 6229
Size: 648 1251 301 281 757 414 1700 359
Cuts at: 6229 6388 518
Size: 159 2042
Fragments arranged by size:
2042 1700 1251 757 648 414 359 301
281 159
MspA1I CmG'CkG
Cuts at: 1606 2182 3233 3736 5023 5065 5328 6005 7153
Size: 576 1051 503 1287 42 263 677 1148
Cuts at: 7153 7398 1606
Size: 245 2120
Fragments arranged by size:
2120 1287 1148 1051 677 576 503 263
245 42
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6421
Fragments arranged by size:
6421 939 552
NdeI CA'TA_TG
Cuts at: 387 3987 387
Size: 3600 4312
NheI G'CTAG_C
Cuts at: 1052 2194 3739 1052
Size: 1142 1545 5225
Fragments arranged by size:
5225 1545 1142
NotI GC'GGCC_GC
Cuts at: 5359 5359
Size: 7912
NspI r_CATG'y
Cuts at: 1132 1229 3091 4171 5270 6815 1132
Size: 97 1862 1080 1099 1545 2229
Fragments arranged by size:
2229 1862 1545 1099 1080 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7912
PciI A'CATG_T
Cuts at: 4167 6811 4167
Size: 2644 5268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3500 4144 4591 5068 1108
Size: 2392 644 447 477 3952
Fragments arranged by size:
3952 2392 644 477 447
PfoI T'CCnGG_A
Cuts at: 3061 3279 4143 3061
Size: 218 864 6830
Fragments arranged by size:
6830 864 218
PmeI GTTT'AAAC
Cuts at: 5216 5216
Size: 7912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5981 6013 7494 7526 5981
Size: 32 1481 32 6367
Fragments arranged by size:
6367 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4621 4621
Size: 7912
PshAI GACnn'nnGTC
Cuts at: 1613 3290 1613
Size: 1677 6235
PsiI TTA'TAA
Cuts at: 5482 5482
Size: 7912
PstI C_TGCA'G
Cuts at: 830 5264 830
Size: 4434 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7912
PvuII CAG'CTG
Cuts at: 2182 3233 3736 5023 5065 2182
Size: 1051 503 1287 42 5029
Fragments arranged by size:
5029 1287 1051 503 42
SacI G_AGCT'C
Cuts at: 2730 5171 2730
Size: 2441 5471
SalI G'TCGA_C
Cuts at: 1936 5254 1936
Size: 3318 4594
SapI GCTCTTCn'nnn_
Cuts at: 2735 3720 2735
Size: 985 6927
SbfI CC_TGCA'GG
Cuts at: 5264 5264
Size: 7912
ScaI AGT'ACT
Cuts at: 3346 6160 7872 3346
Size: 2814 1712 3386
Fragments arranged by size:
3386 2814 1712
SexAI A'CCwGG_T
Cuts at: 3765 3765
Size: 7912
SfcI C'TryA_G
Cuts at: 826 1045 3234 3468 5260 6395 7076 7267 826
Size: 219 2189 234 1792 1135 681 191 1471
Fragments arranged by size:
2189 1792 1471 1135 681 234 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7912
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7912
SmaI CCC'GGG
Cuts at: 1872 3827 5239 1872
Size: 1955 1412 4545
Fragments arranged by size:
4545 1955 1412
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2506 2524 5012 6016 6917
Size: 197 926 207 356 18 2488 1004 901
Cuts at: 6917 7179 820
Size: 262 1553
Fragments arranged by size:
2488 1553 1004 926 901 356 262 207
197 18
SnaBI TAC'GTA
Cuts at: 493 5703 493
Size: 5210 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7912
SphI G_CATG'C
Cuts at: 1132 5270 1132
Size: 4138 3774
SspI AAT'ATT
Cuts at: 5 52 5836 5
Size: 47 5784 2081
Fragments arranged by size:
5784 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7912
StyI C'CwwG_G
Cuts at: 513 1065 2004 2971 4285 4968 5351 513
Size: 552 939 967 1314 683 383 3074
Fragments arranged by size:
3074 1314 967 939 683 552 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3685 5939 5956 6109 6294 6739 325
Size: 1255 2105 2254 17 153 185 445 1498
Fragments arranged by size:
2254 2105 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2203 3256 3344
Size: 274 80 33 51 1443 226 1053 88
Cuts at: 3344 4131 6158 7870 96
Size: 787 2027 1712 138
Fragments arranged by size:
2027 1712 1443 1053 787 274 226 138
88 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2155 2187 2939 2971 5063 5095 7872 7904 2155
Size: 32 752 32 2092 32 2777 32 2163
Fragments arranged by size:
2777 2163 2092 752 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7912
XbaI T'CTAG_A
Cuts at: 5248 5248
Size: 7912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 5062 2494
Size: 2568 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7912
XmaI C'CCGG_G
Cuts at: 1870 3825 5237 1870
Size: 1955 1412 4545
Fragments arranged by size:
4545 1955 1412
XmnI GAAnn'nnTTC
Cuts at: 3612 3714 4119 6041 3612
Size: 102 405 1922 5483
Fragments arranged by size:
5483 1922 405 102
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5720 276
Size: 53 83 186 936 4186 2468
Fragments arranged by size:
4186 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI