Restriction map for
pFN21AE4258
(Circular) (Six-base) MAPSORT of: pFN21AE4258.seq Check: 3092 from: 1 to: 7648
pFN21AE4258 7648 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2204 2204
Size: 7648
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5458 278
Size: 53 83 186 936 3922 2468
Fragments arranged by size:
3922 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2023 3194 4791 4991 1937
Size: 86 1171 1597 200 4594
Fragments arranged by size:
4594 1597 1171 200 86
Acc65I G'GTAC_C
Cuts at: 4920 4969 4920
Size: 49 7599
AclI AA'CG_TT
Cuts at: 1563 5775 6148 1563
Size: 4212 373 3063
Fragments arranged by size:
4212 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2535 4383 4497 5711 7095 7363 1632
Size: 903 1848 114 1214 1384 268 1917
Fragments arranged by size:
1917 1848 1384 1214 903 268 114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7451
AflIII A'CryG_T
Cuts at: 1203 6547 7524 1203
Size: 5344 977 1327
Fragments arranged by size:
5344 1327 977
AgeI A'CCGG_T
Cuts at: 6479 6479
Size: 7648
AhdI GACnn_n'nnGTC
Cuts at: 4798 6377 4798
Size: 1579 6069
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2731 1184
Size: 1547 6101
AlwNI CAG_nnn'CTG
Cuts at: 3368 4865 5391 6963 3368
Size: 1497 526 1572 4053
Fragments arranged by size:
4053 1572 1497 526
ApaLI G'TGCA_C
Cuts at: 5705 6861 5705
Size: 1156 6492
ApoI r'AATT_y
Cuts at: 1493 2649 2915 3184 4544 4629 4957 5183 5335
Size: 1156 266 269 1360 85 328 226 152
Cuts at: 5335 7444 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1360 1156 328 269 266 226
152 85
AseI AT'TA_AT
Cuts at: 160 1032 6202 160
Size: 872 5170 1606
Fragments arranged by size:
5170 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7648
AvaI C'yCGr_G
Cuts at: 1870 1943 4973 1870
Size: 73 3030 4545
Fragments arranged by size:
4545 3030 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4904 4912 4937 4945 4904
Size: 8 25 8 7607
Fragments arranged by size:
7607 25 8 8
BamHI G'GATC_C
Cuts at: 4978 5345 4978
Size: 367 7281
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4920 4969 6424 618
Size: 325 217 627 3133 49 1455 1842
Fragments arranged by size:
3133 1842 1455 627 325 217 49
BanII G_rGCy'C
Cuts at: 1391 2087 3046 3330 4020 4967 1391
Size: 696 959 284 690 947 4072
Fragments arranged by size:
4072 959 947 696 690 284
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 3510 4122 928
Size: 971 433 1178 612 4454
Fragments arranged by size:
4454 1178 971 612 433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5839 5873 1903
Size: 34 3902 34 3678
Fragments arranged by size:
3902 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5540 6750 5540
Size: 1210 6438
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7648
BfrBI ATG'CAT
Cuts at: 2317 2317
Size: 7648
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6259 136
Size: 107 122 71 631 605 4587 1525
Fragments arranged by size:
4587 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2351 7643 2351
Size: 5292 2356
BlpI GC'TnA_GC
Cuts at: 5065 5065
Size: 7648
Bme1580I G_kGCm'C
Cuts at: 1790 5709 6865 1790
Size: 3919 1156 2573
Fragments arranged by size:
3919 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7648
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4091 4457 4558
Size: 416 53 83 399 24 2674 366 101
Cuts at: 4558 4920 5379 6337 442
Size: 362 459 958 1753
Fragments arranged by size:
2674 1753 958 459 416 399 366 362
101 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2858 2898 3154 4214 6308 1239
Size: 1619 40 256 1060 2094 2579
Fragments arranged by size:
2579 2094 1619 1060 256 40
Bpu10I CC'TnA_GC
Cuts at: 3762 4455 3762
Size: 693 6955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3677 3719 3976 5773 6638 6936 3677
Size: 42 257 1797 865 298 4389
Fragments arranged by size:
4389 1797 865 298 257 42
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3787 6311 882
Size: 617 2288 2524 2219
Fragments arranged by size:
2524 2288 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4157 5439 493
Size: 711 2953 1282 2702
Fragments arranged by size:
2953 2702 1282 711
BsaBI GATnn'nnATC
Cuts at: 7642 7642
Size: 7648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5455 5837 6472 275
Size: 53 83 186 936 3922 382 635 1451
Fragments arranged by size:
3922 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2701 3468 6081 6479 6753 6900 7311 2701
Size: 767 2613 398 274 147 411 3038
Fragments arranged by size:
3038 2613 767 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 1178
Size: 30 165 30 687 30 6706
Fragments arranged by size:
6706 687 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2302 1186
Size: 1116 6532
BseYI C'CCAG_C
Cuts at: 2658 4095 6851 7347 2658
Size: 1437 2756 496 2959
Fragments arranged by size:
2959 2756 1437 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 1163
Size: 573 496 6579
Fragments arranged by size:
6579 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5098 5859 6887 1540
Size: 275 186 3097 761 1028 2301
Fragments arranged by size:
3097 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3046 3330 4020 4456 5709 5794 6865 3046
Size: 284 690 436 1253 85 1071 3829
Fragments arranged by size:
3829 1253 1071 690 436 284 85
BsmI GAATG_Cn'
Cuts at: 4188 5159 5252 4188
Size: 971 93 6584
Fragments arranged by size:
6584 971 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 3046 3330 4020 4456 4967 5709
Size: 399 297 959 284 690 436 511 742
Cuts at: 5709 5794 6865 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 959 742 690 511 436 399
297 284 85
BspEI T'CCGG_A
Cuts at: 3468 7311 3468
Size: 3843 3805
BspHI T'CATG_A
Cuts at: 3349 5537 7267 3349
Size: 2188 1730 3730
Fragments arranged by size:
3730 2188 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2204 2573 2633 5003 844
Size: 1360 369 60 2370 3489
Fragments arranged by size:
3489 2370 1360 369 60
BsrBI CCG'CTC
Cuts at: 1394 1823 5535 1394
Size: 429 3712 3507
Fragments arranged by size:
3712 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3345 6143 6317 65
Size: 1183 2097 2798 174 1396
Fragments arranged by size:
2798 2097 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6292 6479 1700
Size: 110 4482 187 2869
Fragments arranged by size:
4482 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4764 96
Size: 4668 2980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7648
BssSI C'ACGA_G
Cuts at: 5708 6720 5708
Size: 1012 6636
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7648
BstBI TT'CG_AA
Cuts at: 2305 6452 2305
Size: 4147 3501
BstEII G'GTnAC_C
Cuts at: 1184 4236 1184
Size: 3052 4596
BstYI r'GATC_y
Cuts at: 1972 2351 3471 4170 4719 4978 5345 5730 5747
Size: 379 1120 699 549 259 367 385 17
Cuts at: 5747 7199 7285 7297 7643 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1120 699 549 385 379 367
346 259 86 17 12
BstZ17I GTA'TAC
Cuts at: 2024 2024
Size: 7648
Bsu36I CC'TnA_GG
Cuts at: 3725 4873 3725
Size: 1148 6500
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7096
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6909
Fragments arranged by size:
6909 683 56
BtsI GCAGTG_nn'
Cuts at: 4526 4674 4863 5164 5958 5978 4526
Size: 148 189 301 794 20 6196
Fragments arranged by size:
6196 794 301 189 148 20
ClaI AT'CG_AT
Cuts at: 1554 3556 1554
Size: 2002 5646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2076 2111 4650 4685 529
Size: 35 1512 35 2539 35 3492
Fragments arranged by size:
3492 2539 1512 35 35 35
DraI TTT'AAA
Cuts at: 2986 4952 2986
Size: 1966 5682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6655 809
Size: 507 5339 1802
Fragments arranged by size:
5339 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5095 5984 7613
Size: 54 1425 50 275 18 3265 889 1629
Cuts at: 7613 8
Size: 43
Fragments arranged by size:
3265 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5095 1537
Size: 275 3283 4090
Fragments arranged by size:
4090 3283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 2600 4003 5578 1353
Size: 1052 195 1403 1575 3423
Fragments arranged by size:
3423 1575 1403 1052 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6235 6609 6755 387
Size: 1064 138 4646 374 146 1280
Fragments arranged by size:
4646 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3044 3328 4018 3044
Size: 284 690 6674
Fragments arranged by size:
6674 690 284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2535 2858 2898 3154 4214 4383 4497
Size: 393 903 323 40 256 1060 169 114
Cuts at: 4497 5711 6308 7095 7363 1239
Size: 1214 597 787 268 1524
Fragments arranged by size:
1524 1214 1060 903 787 597 393 323
268 256 169 114 40
EcoNI CCTnn'n_nnAGG
Cuts at: 2373 3724 2373
Size: 1351 6297
EcoO109I rG'GnC_Cy
Cuts at: 2425 2425
Size: 7648
EcoRI G'AATT_C
Cuts at: 4544 4957 5335 4544
Size: 413 378 6857
Fragments arranged by size:
6857 413 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 7648
FspI TGC'GCA
Cuts at: 6154 6154
Size: 7648
HaeII r_GCGC'y
Cuts at: 1678 3529 6795 1678
Size: 1851 3266 2531
Fragments arranged by size:
3266 2531 1851
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2341 2373 2620 2652 2871
Size: 32 135 32 769 32 247 32 219
Cuts at: 2871 2903 2981 3013 3908 3940 3978 4010 4262
Size: 32 78 32 895 32 38 32 252
Cuts at: 4262 4294 4784 4816 6289 6321 6363 6395 1373
Size: 32 490 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1473 895 769 490 252 247 219
135 78 42 38 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4992 5238 669
Size: 988 281 3054 246 3079
Fragments arranged by size:
3079 3054 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2269 2300 3159 3752 748
Size: 1521 31 859 593 4644
Fragments arranged by size:
4644 1521 859 593 31
HpaI GTT'AAC
Cuts at: 5238 5238
Size: 7648
KpnI G_GTAC'C
Cuts at: 4924 4973 4924
Size: 49 7599
MfeI C'AATT_G
Cuts at: 5247 5247
Size: 7648
MluI A'CGCG_T
Cuts at: 7524 7524
Size: 7648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 3019 6762 6946 1305
Size: 771 943 3743 184 2007
Fragments arranged by size:
3743 2007 943 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5826
Fragments arranged by size:
5826 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4181 5606 5965 6124 518
Size: 648 3015 1425 359 159 2042
Fragments arranged by size:
3015 2042 1425 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2836 3301 3544 4798 5064 5741 6889
Size: 796 434 465 243 1254 266 677 1148
Cuts at: 6889 7134 1606
Size: 245 2120
Fragments arranged by size:
2120 1254 1148 796 677 465 434 266
245 243
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7096
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7648
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7648
NotI GC'GGCC_GC
Cuts at: 5095 5095
Size: 7648
NsiI A_TGCA'T
Cuts at: 2319 2319
Size: 7648
NspI r_CATG'y
Cuts at: 1132 1229 2185 2509 5006 6551 1132
Size: 97 956 324 2497 1545 2229
Fragments arranged by size:
2497 2229 1545 956 324 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7648
PciI A'CATG_T
Cuts at: 6547 6547
Size: 7648
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3692 1108
Size: 2584 5064
PfoI T'CCnGG_A
Cuts at: 4901 4901
Size: 7648
PmeI GTTT'AAAC
Cuts at: 4952 4952
Size: 7648
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5717 5749 7230 7262 5717
Size: 32 1481 32 6103
Fragments arranged by size:
6103 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2425 2425
Size: 7648
PshAI GACnn'nnGTC
Cuts at: 1613 4776 1613
Size: 3163 4485
PsiI TTA'TAA
Cuts at: 5218 5218
Size: 7648
PstI C_TGCA'G
Cuts at: 830 4669 5000 830
Size: 3839 331 3478
Fragments arranged by size:
3839 3478 331
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7648
PvuII CAG'CTG
Cuts at: 2402 2836 3301 3544 4798 2402
Size: 434 465 243 1254 5252
Fragments arranged by size:
5252 1254 465 434 243
SacI G_AGCT'C
Cuts at: 3046 3330 4020 3046
Size: 284 690 6674
Fragments arranged by size:
6674 690 284
SalI G'TCGA_C
Cuts at: 1936 4990 1936
Size: 3054 4594
SbfI CC_TGCA'GG
Cuts at: 5000 5000
Size: 7648
ScaI AGT'ACT
Cuts at: 4446 5896 7608 4446
Size: 1450 1712 4486
Fragments arranged by size:
4486 1712 1450
SfcI C'TryA_G
Cuts at: 826 1045 2565 3475 4244 4665 4788 4996 6131
Size: 219 1520 910 769 421 123 208 1135
Cuts at: 6131 6812 7003 826
Size: 681 191 1471
Fragments arranged by size:
1520 1471 1135 910 769 681 421 219
208 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7648
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7648
SmaI CCC'GGG
Cuts at: 1872 4975 1872
Size: 3103 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3656 3734 3955 5752 6653 6915
Size: 197 926 1713 78 221 1797 901 262
Cuts at: 6915 820
Size: 1553
Fragments arranged by size:
1797 1713 1553 926 901 262 221 197
78
SnaBI TAC'GTA
Cuts at: 493 4157 5439 493
Size: 3664 1282 2702
Fragments arranged by size:
3664 2702 1282
SpeI A'CTAG_T
Cuts at: 152 3094 152
Size: 2942 4706
SphI G_CATG'C
Cuts at: 1132 5006 1132
Size: 3874 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5572 5
Size: 47 3648 1872 2081
Fragments arranged by size:
3648 2081 1872 47
StuI AGG'CCT
Cuts at: 1517 4404 1517
Size: 2887 4761
StyI C'CwwG_G
Cuts at: 513 1065 2146 4300 5087 513
Size: 552 1081 2154 787 3074
Fragments arranged by size:
3074 2154 1081 787 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2145 2362 3778 4134 4439 5675 5692
Size: 1255 565 217 1416 356 305 1236 17
Cuts at: 5692 5845 6030 6475 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 1236 565 445 356 305
217 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4444 4764 4856
Size: 274 80 33 51 1443 2467 320 92
Cuts at: 4856 5894 7606 96
Size: 1038 1712 138
Fragments arranged by size:
2467 1712 1443 1038 320 274 138 92
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2683 2715 3611 3643 3739 3771 4704
Size: 32 524 32 896 32 96 32 933
Cuts at: 4704 4736 7608 7640 2127
Size: 32 2872 32 2135
Fragments arranged by size:
2872 2135 933 896 524 96 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4067 1316
Size: 2751 4897
XbaI T'CTAG_A
Cuts at: 4984 4984
Size: 7648
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2737 4347 2167
Size: 570 1610 5468
Fragments arranged by size:
5468 1610 570
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7648
XmaI C'CCGG_G
Cuts at: 1870 4973 1870
Size: 3103 4545
XmnI GAAnn'nnTTC
Cuts at: 2846 5777 2846
Size: 2931 4717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5456 276
Size: 53 83 186 936 3922 2468
Fragments arranged by size:
3922 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
BsiWI BsmBI BstXI DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI