Restriction map for
pFN21AE4256
(Circular) (Six-base) MAPSORT of: pFN21AE4256.seq Check: 4069 from: 1 to: 7780
pFN21AE4256 7780 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3017 3017
Size: 7780
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5590 278
Size: 53 83 186 936 4054 2468
Fragments arranged by size:
4054 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5061 5123 1937
Size: 3124 62 4594
Fragments arranged by size:
4594 3124 62
Acc65I G'GTAC_C
Cuts at: 5101 5101
Size: 7780
AclI AA'CG_TT
Cuts at: 1563 5907 6280 1563
Size: 4344 373 3063
Fragments arranged by size:
4344 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2896 3949 4300 5843 7227 7495 1632
Size: 419 845 1053 351 1543 1384 268 1917
Fragments arranged by size:
1917 1543 1384 1053 845 419 351 268
AfeI AGC'GCT
Cuts at: 4177 4177
Size: 7780
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7583
AflIII A'CryG_T
Cuts at: 1203 6679 7656 1203
Size: 5476 977 1327
Fragments arranged by size:
5476 1327 977
AgeI A'CCGG_T
Cuts at: 6611 6611
Size: 7780
AhdI GACnn_n'nnGTC
Cuts at: 4045 6509 4045
Size: 2464 5316
AleI CACnn'nnGTG
Cuts at: 1166 3011 1166
Size: 1845 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5015 1184
Size: 3831 3949
AlwNI CAG_nnn'CTG
Cuts at: 2440 3452 3472 4423 4717 5008 5026 5523 7095
Size: 1012 20 951 294 291 18 497 1572
Cuts at: 7095 2440
Size: 3125
Fragments arranged by size:
3125 1572 1012 951 497 294 291 20
18
ApaI G_GGCC'C
Cuts at: 4122 4122
Size: 7780
ApaLI G'TGCA_C
Cuts at: 2461 2498 5051 5837 6993 2461
Size: 37 2553 786 1156 3248
Fragments arranged by size:
3248 2553 1156 786 37
ApoI r'AATT_y
Cuts at: 1493 3458 5089 5315 5467 7576 1493
Size: 1965 1631 226 152 2109 1697
Fragments arranged by size:
2109 1965 1697 1631 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6334 160
Size: 872 5302 1606
Fragments arranged by size:
5302 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7780
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 2279 2319 3088 3223 3441 5073
Size: 73 288 48 40 769 135 218 1632
Cuts at: 5073 5105 1870
Size: 32 4545
Fragments arranged by size:
4545 1632 769 288 218 135 73 48
40 32
BamHI G'GATC_C
Cuts at: 5011 5110 5477 5011
Size: 99 367 7314
Fragments arranged by size:
7314 367 99
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2382 3092 3678 3776 3893
Size: 325 217 627 595 710 586 98 117
Cuts at: 3893 5101 6556 618
Size: 1208 1455 1842
Fragments arranged by size:
1842 1455 1208 710 627 595 586 325
217 117 98
BanII G_rGCy'C
Cuts at: 1391 2835 2979 3794 3938 4122 5099 1391
Size: 1444 144 815 144 184 977 4072
Fragments arranged by size:
4072 1444 977 815 184 144 144
BbeI G_GCGC'C
Cuts at: 2386 3897 2386
Size: 1511 6269
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 4493 928
Size: 971 140 2454 4215
Fragments arranged by size:
4215 2454 971 140
BbvCI CC'TCA_GC
Cuts at: 2796 3908 4211 4844 2796
Size: 1112 303 633 5732
Fragments arranged by size:
5732 1112 633 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2945 2979 4648 4682 5971 6005 1903
Size: 34 1008 34 1669 34 1289 34 3678
Fragments arranged by size:
3678 1669 1289 1008 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5672 6882 5672
Size: 1210 6570
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2429 2543 2763
Size: 107 122 71 631 605 757 114 220
Cuts at: 2763 3110 3527 3851 4126 4501 6391 136
Size: 347 417 324 275 375 1890 1525
Fragments arranged by size:
1890 1525 757 631 605 417 375 347
324 275 220 122 114 107 71
BglII A'GATC_T
Cuts at: 7775 7775
Size: 7780
BlpI GC'TnA_GC
Cuts at: 2093 3884 4130 4179 5197 2093
Size: 1791 246 49 1018 4676
Fragments arranged by size:
4676 1791 1018 246 49
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2465 2502 3089 3781 4122 4219 5055
Size: 620 55 37 587 692 341 97 836
Cuts at: 5055 5841 6997 1790
Size: 786 1156 2573
Fragments arranged by size:
2573 1156 836 786 692 620 587 341
97 55 37
BmgBI CAC'GTC
Cuts at: 1318 2314 4042 1318
Size: 996 1728 5056
Fragments arranged by size:
5056 1728 996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2353 4229 5511
Size: 416 53 83 399 24 936 1876 1282
Cuts at: 5511 6469 442
Size: 958 1753
Fragments arranged by size:
1876 1753 1282 958 936 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2306 2825 2993 3008 3938 4073 4787
Size: 821 246 519 168 15 930 135 714
Cuts at: 4787 4973 6440 1239
Size: 186 1467 2579
Fragments arranged by size:
2579 1467 930 821 714 519 246 186
168 135 15
Bpu10I CC'TnA_GC
Cuts at: 2441 2796 3908 4211 4844 2441
Size: 355 1112 303 633 5377
Fragments arranged by size:
5377 1112 633 355 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4373 4916 5905 6770 7068 4373
Size: 543 989 865 298 5085
Fragments arranged by size:
5085 989 865 543 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3049 6443 882
Size: 617 1550 3394 2219
Fragments arranged by size:
3394 2219 1550 617
BsaAI yAC'GTr
Cuts at: 493 1204 4192 5571 493
Size: 711 2988 1379 2702
Fragments arranged by size:
2988 2702 1379 711
BsaBI GATnn'nnATC
Cuts at: 2813 7774 2813
Size: 4961 2819
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2383 2826 2841 3073
Size: 53 83 186 936 850 443 15 232
Cuts at: 3073 3150 3575 3830 3894 5587 5969 6604 275
Size: 77 425 255 64 1693 382 635 1451
Fragments arranged by size:
1693 1451 936 850 635 443 425 382
255 232 186 83 77 64 53 15
BsaWI w'CCGG_w
Cuts at: 3426 3538 4482 6213 6611 6885 7032 7443 3426
Size: 112 944 1731 398 274 147 411 3763
Fragments arranged by size:
3763 1731 944 411 398 274 147 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2195 3029 3059 4151
Size: 30 165 30 762 30 834 30 1092
Cuts at: 4151 4181 4304 4334 4760 4790 5054 5084 1178
Size: 30 123 30 426 30 264 30 3874
Fragments arranged by size:
3874 1092 834 762 426 264 165 123
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2738 3896 3928 4070 4346 4349 4361
Size: 1117 435 1158 32 142 276 3 12
Cuts at: 4361 1186
Size: 4605
Fragments arranged by size:
4605 1158 1117 435 276 142 32 12
3
BseYI C'CCAG_C
Cuts at: 2205 2505 2967 3599 3635 4466 6983 7479 2205
Size: 300 462 632 36 831 2517 496 2506
Fragments arranged by size:
2517 2506 831 632 496 462 300 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 2498 4223 4316 5021 1163
Size: 573 699 63 1725 93 705 3922
Fragments arranged by size:
3922 1725 705 699 573 93 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2347 4987 5230 5991 7019 1540
Size: 275 186 346 2640 243 761 1028 2301
Fragments arranged by size:
2640 2301 1028 761 346 275 243 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2465 2502 2835 2979 3312 4185 4835 5055
Size: 137 37 333 144 333 873 650 220
Cuts at: 5055 5841 5926 6997 2328
Size: 786 85 1071 3111
Fragments arranged by size:
3111 1071 873 786 650 333 333 220
144 137 85 37
BsmI GAATG_Cn'
Cuts at: 5291 5384 5291
Size: 93 7687
BsmBI CGTCTCn'nnnn_
Cuts at: 2304 3821 2304
Size: 1517 6263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2410 2465 2502 2835 2979 3089
Size: 399 538 82 55 37 333 144 110
Cuts at: 3089 3312 3781 3794 3938 4122 4185 4219 4835
Size: 223 469 13 144 184 63 34 616
Cuts at: 4835 5055 5099 5841 5926 6997 1391
Size: 220 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 538 469 399 333
223 220 184 144 144 110 85 82
63 55 44 37 34 13
BspEI T'CCGG_A
Cuts at: 3426 3538 4482 7443 3426
Size: 112 944 2961 3763
Fragments arranged by size:
3763 2961 944 112
BspHI T'CATG_A
Cuts at: 5669 7399 5669
Size: 1730 6050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3017 5034 5135 844
Size: 2173 2017 101 3489
Fragments arranged by size:
3489 2173 2017 101
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 5667 1394
Size: 429 265 3579 3507
Fragments arranged by size:
3579 3507 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 6275 6449 65
Size: 1183 5027 174 1396
Fragments arranged by size:
5027 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2346 2570 3702 4495 6424 6611 1700
Size: 110 536 224 1132 793 1929 187 2869
Fragments arranged by size:
2869 1929 1132 793 536 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 2556 96
Size: 2460 5320
BssHII G'CGCG_C
Cuts at: 1925 2526 2600 1925
Size: 601 74 7105
Fragments arranged by size:
7105 601 74
BssSI C'ACGA_G
Cuts at: 2080 2675 3947 5840 6852 2080
Size: 595 1272 1893 1012 3008
Fragments arranged by size:
3008 1893 1272 1012 595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3064 5026 794
Size: 2270 1962 3548
Fragments arranged by size:
3548 2270 1962
BstBI TT'CG_AA
Cuts at: 6584 6584
Size: 7780
BstEII G'GTnAC_C
Cuts at: 1184 4354 1184
Size: 3170 4610
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 3013 4106 4448 2743
Size: 270 1093 342 6075
Fragments arranged by size:
6075 1093 342 270
BstYI r'GATC_y
Cuts at: 1972 2727 4059 4479 4507 4746 4824 4884 5011
Size: 755 1332 420 28 239 78 60 127
Cuts at: 5011 5110 5477 5862 5879 7331 7417 7429 7775
Size: 99 367 385 17 1452 86 12 346
Cuts at: 7775 1972
Size: 1977
Fragments arranged by size:
1977 1452 1332 755 420 385 367 346
239 127 99 86 78 60 28 17
12
Bsu36I CC'TnA_GG
Cuts at: 4001 4001
Size: 7780
BtgI C'CryG_G
Cuts at: 513 1065 2004 2152 2270 3198 3327 3555 3925
Size: 552 939 148 118 928 129 228 370
Cuts at: 3925 3982 4203 4740 513
Size: 57 221 537 3553
Fragments arranged by size:
3553 939 928 552 537 370 228 221
148 129 118 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3626 487
Size: 683 56 2400 4641
Fragments arranged by size:
4641 2400 683 56
BtsI GCAGTG_nn'
Cuts at: 3804 4111 4441 4816 5015 5296 6090 6110 3804
Size: 307 330 375 199 281 794 20 5474
Fragments arranged by size:
5474 794 375 330 307 281 199 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7780
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4500 4535 529
Size: 35 3936 35 3774
Fragments arranged by size:
3936 3774 35 35
DraI TTT'AAA
Cuts at: 5084 5084
Size: 7780
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6787 809
Size: 507 5471 1802
Fragments arranged by size:
5471 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2247 2344
Size: 54 1425 50 275 18 177 240 97
Cuts at: 2344 2388 2514 2745 3003 3291 3985 4065 4497
Size: 44 126 231 258 288 694 80 432
Cuts at: 4497 4984 5227 6116 7745 8
Size: 487 243 889 1629 43
Fragments arranged by size:
1629 1425 889 694 487 432 288 275
258 243 240 231 177 126 97 80
54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2344 4984 5227 1537
Size: 275 532 2640 243 4090
Fragments arranged by size:
4090 2640 532 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2140 2261 5710 1353
Size: 787 121 3449 3423
Fragments arranged by size:
3449 3423 787 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2356 2781 6367 6741 6887 387
Size: 1064 138 767 425 3586 374 146 1280
Fragments arranged by size:
3586 1280 1064 767 425 374 146 138
EcoICRI GAG'CTC
Cuts at: 2833 2977 2833
Size: 144 7636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2060 2306 2825 2896 2993 3008
Size: 393 419 9 246 519 71 97 15
Cuts at: 3008 3938 3949 4073 4300 4787 4973 5843 6440
Size: 930 11 124 227 487 186 870 597
Cuts at: 6440 7227 7495 1239
Size: 787 268 1524
Fragments arranged by size:
1524 930 870 787 597 519 487 419
393 268 246 227 186 124 97 71
15 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 3427 3947 4067 4157 2720
Size: 707 520 120 90 6343
Fragments arranged by size:
6343 707 520 120 90
EcoO109I rG'GnC_Cy
Cuts at: 2236 2800 2931 3469 4118 4150 4263 4945 5005
Size: 564 131 538 649 32 113 682 60
Cuts at: 5005 5023 2236
Size: 18 4993
Fragments arranged by size:
4993 682 649 564 538 131 113 60
32 18
EcoRI G'AATT_C
Cuts at: 5089 5467 5089
Size: 378 7402
FspI TGC'GCA
Cuts at: 2669 6286 2669
Size: 3617 4163
FspAI rTGC'GCAy
Cuts at: 2669 2669
Size: 7780
HaeII r_GCGC'y
Cuts at: 1678 2129 2340 2386 2440 2487 2513 2549 2571
Size: 451 211 46 54 47 26 36 22
Cuts at: 2571 3897 4179 6927 1678
Size: 1326 282 2748 2531
Fragments arranged by size:
2748 2531 1326 451 282 211 54 47
46 36 26 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 3029 3061 4031
Size: 32 135 32 671 32 754 32 970
Cuts at: 4031 4063 4240 4272 4304 4336 4699 4731 4760
Size: 32 177 32 32 32 363 32 29
Cuts at: 4760 4792 6421 6453 6495 6527 1373
Size: 32 1629 32 42 32 2626
Fragments arranged by size:
2626 1629 970 754 671 363 177 135
42 32 32 32 32 32 32 32
32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 3550 5124 5370 669
Size: 988 281 1612 1574 246 3079
Fragments arranged by size:
3079 1612 1574 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7780
HpaI GTT'AAC
Cuts at: 3550 5370 3550
Size: 1820 5960
KasI G'GCGC_C
Cuts at: 2382 3893 2382
Size: 1511 6269
KpnI G_GTAC'C
Cuts at: 5105 5105
Size: 7780
MfeI C'AATT_G
Cuts at: 3758 5379 3758
Size: 1621 6159
MluI A'CGCG_T
Cuts at: 7656 7656
Size: 7780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 6894 7078 1305
Size: 834 4755 184 2007
Fragments arranged by size:
4755 2007 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 3005 3293 10
Size: 54 1425 343 915 258 288 4497
Fragments arranged by size:
4497 1425 915 343 288 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3011 3749 4202 4446 5738 6097 6256
Size: 648 1845 738 453 244 1292 359 159
Cuts at: 6256 518
Size: 2042
Fragments arranged by size:
2042 1845 1292 738 648 453 359 244
159
MspA1I CmG'CkG
Cuts at: 1606 2154 2205 2272 2505 2640 2971 3336 3841
Size: 548 51 67 233 135 331 365 505
Cuts at: 3841 3977 4781 4798 5196 5873 7021 7266 1606
Size: 136 804 17 398 677 1148 245 2120
Fragments arranged by size:
2120 1148 804 677 548 505 398 365
331 245 233 136 135 67 51 17
NaeI GCC'GGC
Cuts at: 2348 2572 3704 4497 2348
Size: 224 1132 793 5631
Fragments arranged by size:
5631 1132 793 224
NarI GG'CG_CC
Cuts at: 2383 3894 2383
Size: 1511 6269
NcoI C'CATG_G
Cuts at: 513 1065 2004 3198 3555 4203 4740 513
Size: 552 939 1194 357 648 537 3553
Fragments arranged by size:
3553 1194 939 648 552 537 357
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7780
NgoMIV G'CCGG_C
Cuts at: 2346 2570 3702 4495 2346
Size: 224 1132 793 5631
Fragments arranged by size:
5631 1132 793 224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7780
NotI GC'GGCC_GC
Cuts at: 5227 5227
Size: 7780
NspI r_CATG'y
Cuts at: 1132 1229 3067 3487 3916 5138 6683 1132
Size: 97 1838 420 429 1222 1545 2229
Fragments arranged by size:
2229 1838 1545 1222 429 420 97
PasI CC'CwG_GG
Cuts at: 1348 2935 1348
Size: 1587 6193
PciI A'CATG_T
Cuts at: 6679 6679
Size: 7780
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3138 3472 3931 4423 1108
Size: 2030 334 459 492 4465
Fragments arranged by size:
4465 2030 492 459 334
PfoI T'CCnGG_A
Cuts at: 4764 4764
Size: 7780
PmeI GTTT'AAAC
Cuts at: 5084 5084
Size: 7780
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5849 5881 7362 7394 5849
Size: 32 1481 32 6235
Fragments arranged by size:
6235 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2236 2931 3469 4263 5023 2236
Size: 695 538 794 760 4993
Fragments arranged by size:
4993 794 760 695 538
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7780
PsiI TTA'TAA
Cuts at: 5350 5350
Size: 7780
PspOMI G'GGCC_C
Cuts at: 4118 4118
Size: 7780
PstI C_TGCA'G
Cuts at: 830 3840 4812 5022 5031 5132 830
Size: 3010 972 210 9 101 3478
Fragments arranged by size:
3478 3010 972 210 101 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7780
PvuII CAG'CTG
Cuts at: 2205 2505 2971 3841 3977 4781 4798 2205
Size: 300 466 870 136 804 17 5187
Fragments arranged by size:
5187 870 804 466 300 136 17
SacI G_AGCT'C
Cuts at: 2835 2979 2835
Size: 144 7636
SacII CC_GC'GG
Cuts at: 2155 2273 2155
Size: 118 7662
SalI G'TCGA_C
Cuts at: 1936 5122 1936
Size: 3186 4594
SanDI GG'GwC_CC
Cuts at: 2931 2931
Size: 7780
SbfI CC_TGCA'GG
Cuts at: 5132 5132
Size: 7780
ScaI AGT'ACT
Cuts at: 6028 7740 6028
Size: 1712 6068
SexAI A'CCwGG_T
Cuts at: 2715 2715
Size: 7780
SfcI C'TryA_G
Cuts at: 826 1045 3836 4154 4171 4808 5018 5027 5128
Size: 219 2791 318 17 637 210 9 101
Cuts at: 5128 6263 6944 7135 826
Size: 1135 681 191 1471
Fragments arranged by size:
2791 1471 1135 681 637 318 219 210
191 101 17 9
SfoI GGC'GCC
Cuts at: 2384 3895 2384
Size: 1511 6269
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7780
SgrAI Cr'CCGG_yG
Cuts at: 1700 2570 1700
Size: 870 6910
SmaI CCC'GGG
Cuts at: 1872 2321 3090 5075 5107 1872
Size: 449 769 1985 32 4545
Fragments arranged by size:
4545 1985 769 449 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4388 4931 5884 6785 7047 820
Size: 197 926 2445 543 953 901 262 1553
Fragments arranged by size:
2445 1553 953 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 5571 493
Size: 5078 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7780
SphI G_CATG'C
Cuts at: 1132 3487 3916 5138 1132
Size: 2355 429 1222 3774
Fragments arranged by size:
3774 2355 1222 429
SspI AAT'ATT
Cuts at: 5 52 5704 5
Size: 47 5652 2081
Fragments arranged by size:
5652 2081 47
StuI AGG'CCT
Cuts at: 1517 3530 1517
Size: 2013 5767
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3198 3531 3555 4146 4203
Size: 552 939 48 1146 333 24 591 57
Cuts at: 4203 4740 5219 513
Size: 537 479 3074
Fragments arranged by size:
3074 1146 939 591 552 537 479 333
57 48 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 4373 4454 5807 5824 5977 6162
Size: 1255 738 2055 81 1353 17 153 185
Cuts at: 6162 6607 325
Size: 445 1498
Fragments arranged by size:
2055 1498 1353 1255 738 445 185 153
81 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 4167 4338
Size: 274 80 33 51 1443 579 1611 171
Cuts at: 4338 4719 6026 7738 96
Size: 381 1307 1712 138
Fragments arranged by size:
1712 1611 1443 1307 579 381 274 171
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4209 4241 4569 4601 7740 7772 4209
Size: 32 328 32 3139 32 4217
Fragments arranged by size:
4217 3139 328 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3041 4409 1316
Size: 1725 1368 4687
Fragments arranged by size:
4687 1725 1368
XbaI T'CTAG_A
Cuts at: 5116 5116
Size: 7780
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2759 3529 4762 2759
Size: 770 1233 5777
Fragments arranged by size:
5777 1233 770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7780
XmaI C'CCGG_G
Cuts at: 1870 2319 3088 5073 5105 1870
Size: 449 769 1985 32 4545
Fragments arranged by size:
4545 1985 769 449 32
XmnI GAAnn'nnTTC
Cuts at: 3962 5909 3962
Size: 1947 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5588 276
Size: 53 83 186 936 4054 2468
Fragments arranged by size:
4054 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
DraIII EcoRV FalI FseI NruI NsiI PacI PmlI
PspXI PsrI RsrII SapI SfiI SrfI SwaI