Restriction map for
pFN21AE4255
(Circular) (Six-base) MAPSORT of: pFN21AE4255.seq Check: 2129 from: 1 to: 7771
pFN21AE4255 7771 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5581 278
Size: 53 83 186 936 4045 2468
Fragments arranged by size:
4045 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3736 5114 1937
Size: 1799 1378 4594
Fragments arranged by size:
4594 1799 1378
Acc65I G'GTAC_C
Cuts at: 5092 5092
Size: 7771
AclI AA'CG_TT
Cuts at: 1563 5898 6271 1563
Size: 4335 373 3063
Fragments arranged by size:
4335 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 2723 3045 4101 4704 5834 7218 7486
Size: 702 389 322 1056 603 1130 1384 268
Cuts at: 7486 1632
Size: 1917
Fragments arranged by size:
1917 1384 1130 1056 702 603 389 322
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7574
AflIII A'CryG_T
Cuts at: 1203 3110 3380 6670 7647 1203
Size: 1907 270 3290 977 1327
Fragments arranged by size:
3290 1907 1327 977 270
AgeI A'CCGG_T
Cuts at: 6602 6602
Size: 7771
AhdI GACnn_n'nnGTC
Cuts at: 6500 6500
Size: 7771
AleI CACnn'nnGTG
Cuts at: 1166 2750 1166
Size: 1584 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7771
AlwNI CAG_nnn'CTG
Cuts at: 5514 7086 5514
Size: 1572 6199
ApaLI G'TGCA_C
Cuts at: 2697 2912 5828 6984 2697
Size: 215 2916 1156 3484
Fragments arranged by size:
3484 2916 1156 215
ApoI r'AATT_y
Cuts at: 1493 2336 3998 4115 4128 4625 4744 4838 5080
Size: 843 1662 117 13 497 119 94 242
Cuts at: 5080 5306 5458 7567 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1662 843 497 242 226 152
119 117 94 13
AseI AT'TA_AT
Cuts at: 160 1032 4122 6325 160
Size: 872 3090 2203 1606
Fragments arranged by size:
3090 2203 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7771
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 3119 3386 5096 1870
Size: 73 123 1053 267 1710 4545
Fragments arranged by size:
4545 1710 1053 267 123 73
BamHI G'GATC_C
Cuts at: 2544 5101 5468 2544
Size: 2557 367 4847
Fragments arranged by size:
4847 2557 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 5092 6547 618
Size: 325 217 627 234 3071 1455 1842
Fragments arranged by size:
3071 1842 1455 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2578 3409 5090 1391
Size: 1187 831 1681 4072
Fragments arranged by size:
4072 1681 1187 831
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 7771
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2284 3033 3811 928
Size: 971 385 749 778 4888
Fragments arranged by size:
4888 971 778 749 385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5962 5996 1903
Size: 34 4025 34 3678
Fragments arranged by size:
4025 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2804 3091 4227 4757 5663 6873 2804
Size: 287 1136 530 906 1210 3702
Fragments arranged by size:
3702 1210 1136 906 530 287
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7771
BfrBI ATG'CAT
Cuts at: 3478 3970 3478
Size: 492 7279
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 6382 136
Size: 107 122 71 631 605 901 3809 1525
Fragments arranged by size:
3809 1525 901 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7766 7766
Size: 7771
BlpI GC'TnA_GC
Cuts at: 2073 5188 2073
Size: 3115 4656
Bme1580I G_kGCm'C
Cuts at: 1790 2701 2916 5832 6988 1790
Size: 911 215 2916 1156 2573
Fragments arranged by size:
2916 2573 1156 911 215
BmgBI CAC'GTC
Cuts at: 1318 4862 1318
Size: 3544 4227
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2660 3843 4504
Size: 416 53 83 399 24 1243 1183 661
Cuts at: 4504 5502 6460 442
Size: 998 958 1753
Fragments arranged by size:
1753 1243 1183 998 958 661 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7771
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2521 4487 2122
Size: 399 1966 5406
Fragments arranged by size:
5406 1966 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2014 2558 3306 4164 6431 1239
Size: 775 544 748 858 2267 2579
Fragments arranged by size:
2579 2267 858 775 748 544
Bpu10I CC'TnA_GC
Cuts at: 2556 2556
Size: 7771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5896 6761 7059 5896
Size: 865 298 6608
Fragments arranged by size:
6608 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6434 882
Size: 617 4935 2219
Fragments arranged by size:
4935 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3113 3381 5562 493
Size: 711 1909 268 2181 2702
Fragments arranged by size:
2702 2181 1909 711 268
BsaBI GATnn'nnATC
Cuts at: 7765 7765
Size: 7771
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 5578 5960 6595
Size: 53 83 186 936 489 3556 382 635
Cuts at: 6595 275
Size: 1451
Fragments arranged by size:
3556 1451 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2977 6204 6602 6876 7023 7434 2977
Size: 3227 398 274 147 411 3314
Fragments arranged by size:
3314 3227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 3100 3130 1178
Size: 30 165 30 1002 30 665 30 5819
Fragments arranged by size:
5819 1002 665 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7771
BseYI C'CCAG_C
Cuts at: 2167 2323 2482 2616 2806 6974 7470 2167
Size: 156 159 134 190 4168 496 2468
Fragments arranged by size:
4168 2468 496 190 159 156 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5221 5982 7010 1540
Size: 275 186 3220 761 1028 2301
Fragments arranged by size:
3220 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2701 2916 3118 3409 5832 5917 6988 2262
Size: 439 215 202 291 2423 85 1071 3045
Fragments arranged by size:
3045 2423 1071 439 291 215 202 85
BsmI GAATG_Cn'
Cuts at: 4296 5282 5375 4296
Size: 986 93 6692
Fragments arranged by size:
6692 986 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4606 4606
Size: 7771
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2578 2701 2916 3118 3409 5090
Size: 399 472 316 123 215 202 291 1681
Cuts at: 5090 5832 5917 6988 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1681 1071 742 472 399 316 291
215 202 123 85
BspEI T'CCGG_A
Cuts at: 2977 7434 2977
Size: 4457 3314
BspHI T'CATG_A
Cuts at: 5660 7390 5660
Size: 1730 6041
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3844 5126 844
Size: 3000 1282 3489
Fragments arranged by size:
3489 3000 1282
BsrBI CCG'CTC
Cuts at: 1394 1823 5658 1394
Size: 429 3835 3507
Fragments arranged by size:
3835 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6266 6440 65
Size: 1183 5018 174 1396
Fragments arranged by size:
5018 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6415 6602 1700
Size: 110 4605 187 2869
Fragments arranged by size:
4605 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7771
BssHII G'CGCG_C
Cuts at: 1925 2181 1925
Size: 256 7515
BssSI C'ACGA_G
Cuts at: 2140 2240 5831 6843 2140
Size: 100 3591 1012 3068
Fragments arranged by size:
3591 3068 1012 100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2747 794
Size: 1953 5818
BstBI TT'CG_AA
Cuts at: 6575 6575
Size: 7771
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7771
BstXI CCAn_nnnn'nTGG
Cuts at: 3307 4543 3307
Size: 1236 6535
BstYI r'GATC_y
Cuts at: 1972 2544 4530 5101 5468 5853 5870 7322 7408
Size: 572 1986 571 367 385 17 1452 86
Cuts at: 7408 7420 7766 1972
Size: 12 346 1977
Fragments arranged by size:
1986 1977 1452 572 571 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2473 3159 513
Size: 552 1408 686 5125
Fragments arranged by size:
5125 1408 686 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7032
Fragments arranged by size:
7032 683 56
BtsI GCAGTG_nn'
Cuts at: 2745 3186 5287 6081 6101 2745
Size: 441 2101 794 20 4415
Fragments arranged by size:
4415 2101 794 441 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7771
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7736
DraI TTT'AAA
Cuts at: 3270 4320 4395 5075 3270
Size: 1050 75 680 5966
Fragments arranged by size:
5966 1050 680 75
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6778 809
Size: 507 5462 1802
Fragments arranged by size:
5462 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 5218 6107
Size: 54 1425 50 275 18 258 3130 889
Cuts at: 6107 7736 8
Size: 1629 43
Fragments arranged by size:
3130 1629 1425 889 275 258 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5218 1537
Size: 275 3406 4090
Fragments arranged by size:
4090 3406 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2825 3414 4176 4415 5701 1353
Size: 1472 589 762 239 1286 3423
Fragments arranged by size:
3423 1472 1286 762 589 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6358 6732 6878 387
Size: 1064 138 4769 374 146 1280
Fragments arranged by size:
4769 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3407 3407
Size: 7771
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2014 2334 2558 2723 3045 3306 4101
Size: 393 382 320 224 165 322 261 795
Cuts at: 4101 4164 4704 5834 6431 7218 7486 1239
Size: 63 540 1130 597 787 268 1524
Fragments arranged by size:
1524 1130 795 787 597 540 393 382
322 320 268 261 224 165 63
EcoO109I rG'GnC_Cy
Cuts at: 2134 2532 2983 2134
Size: 398 451 6922
Fragments arranged by size:
6922 451 398
EcoRI G'AATT_C
Cuts at: 2336 4744 5080 5458 2336
Size: 2408 336 378 4649
Fragments arranged by size:
4649 2408 378 336
FspI TGC'GCA
Cuts at: 6277 6277
Size: 7771
HaeII r_GCGC'y
Cuts at: 1678 2025 2047 2104 6918 1678
Size: 347 22 57 4814 2531
Fragments arranged by size:
4814 2531 347 57 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2780 2812 3076 3108 4266
Size: 32 135 32 1208 32 264 32 1158
Cuts at: 4266 4298 5031 5063 6412 6444 6486 6518 1373
Size: 32 733 32 1349 32 42 32 2626
Fragments arranged by size:
2626 1349 1208 1158 733 264 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3737 4513 5115 5361 669
Size: 988 281 1407 392 776 602 246 3079
Fragments arranged by size:
3079 1407 988 776 602 392 281 246
HindIII A'AGCT_T
Cuts at: 748 3435 748
Size: 2687 5084
HpaI GTT'AAC
Cuts at: 3345 5361 3345
Size: 2016 5755
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 7771
KpnI G_GTAC'C
Cuts at: 5096 5096
Size: 7771
MfeI C'AATT_G
Cuts at: 5370 5370
Size: 7771
MluI A'CGCG_T
Cuts at: 7647 7647
Size: 7771
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3798 6885 7069 1305
Size: 2493 3087 184 2007
Fragments arranged by size:
3087 2493 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5949
Fragments arranged by size:
5949 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2750 3305 3675 3965 4541 5729 6088
Size: 648 1584 555 370 290 576 1188 359
Cuts at: 6088 6247 518
Size: 159 2042
Fragments arranged by size:
2042 1584 1188 648 576 555 370 359
290 159
MspA1I CmG'CkG
Cuts at: 1606 2094 2167 2323 2482 2641 2730 2806 3604
Size: 488 73 156 159 159 89 76 798
Cuts at: 3604 3640 5187 5864 7012 7257 1606
Size: 36 1547 677 1148 245 2120
Fragments arranged by size:
2120 1547 1148 798 677 488 245 159
159 156 89 76 73 36
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 7771
NcoI C'CATG_G
Cuts at: 513 1065 2473 3159 513
Size: 552 1408 686 5125
Fragments arranged by size:
5125 1408 686 552
NdeI CA'TA_TG
Cuts at: 387 2908 3070 387
Size: 2521 162 5088
Fragments arranged by size:
5088 2521 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7771
NotI GC'GGCC_GC
Cuts at: 5218 5218
Size: 7771
NsiI A_TGCA'T
Cuts at: 3480 3972 3480
Size: 492 7279
NspI r_CATG'y
Cuts at: 1132 1229 3964 4240 4877 5129 6674 1132
Size: 97 2735 276 637 252 1545 2229
Fragments arranged by size:
2735 2229 1545 637 276 252 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7771
PciI A'CATG_T
Cuts at: 6670 6670
Size: 7771
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 3160 4202 1108
Size: 1317 735 1042 4677
Fragments arranged by size:
4677 1317 1042 735
PmeI GTTT'AAAC
Cuts at: 5075 5075
Size: 7771
PmlI CAC'GTG
Cuts at: 3113 3113
Size: 7771
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5840 5872 7353 7385 5840
Size: 32 1481 32 6226
Fragments arranged by size:
6226 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2532 2134
Size: 398 7373
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7771
PsiI TTA'TAA
Cuts at: 5341 5341
Size: 7771
PstI C_TGCA'G
Cuts at: 830 2043 2085 3841 5123 830
Size: 1213 42 1756 1282 3478
Fragments arranged by size:
3478 1756 1282 1213 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7771
PvuII CAG'CTG
Cuts at: 2167 2323 2482 2641 2730 2806 3640 2167
Size: 156 159 159 89 76 834 6298
Fragments arranged by size:
6298 834 159 159 156 89 76
SacI G_AGCT'C
Cuts at: 3409 3409
Size: 7771
SalI G'TCGA_C
Cuts at: 1936 3735 5113 1936
Size: 1799 1378 4594
Fragments arranged by size:
4594 1799 1378
SapI GCTCTTCn'nnn_
Cuts at: 3414 3414
Size: 7771
SbfI CC_TGCA'GG
Cuts at: 5123 5123
Size: 7771
ScaI AGT'ACT
Cuts at: 3196 6019 7731 3196
Size: 2823 1712 3236
Fragments arranged by size:
3236 2823 1712
SexAI A'CCwGG_T
Cuts at: 3501 3501
Size: 7771
SfcI C'TryA_G
Cuts at: 826 1045 2039 2081 3837 5119 6254 6935 7126
Size: 219 994 42 1756 1282 1135 681 191
Cuts at: 7126 826
Size: 1471
Fragments arranged by size:
1756 1471 1282 1135 994 681 219 191
42
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 7771
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7771
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7771
SmaI CCC'GGG
Cuts at: 1872 2068 3121 5098 1872
Size: 196 1053 1977 4545
Fragments arranged by size:
4545 1977 1053 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3386 5875 6776 7038 820
Size: 197 926 1443 2489 901 262 1553
Fragments arranged by size:
2489 1553 1443 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5562 493
Size: 5069 2702
SpeI A'CTAG_T
Cuts at: 152 3101 152
Size: 2949 4822
SphI G_CATG'C
Cuts at: 1132 4877 5129 1132
Size: 3745 252 3774
Fragments arranged by size:
3774 3745 252
SspI AAT'ATT
Cuts at: 5 52 4966 5695 5
Size: 47 4914 729 2081
Fragments arranged by size:
4914 2081 729 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7771
StyI C'CwwG_G
Cuts at: 513 1065 2473 3159 4821 5210 513
Size: 552 1408 686 1662 389 3074
Fragments arranged by size:
3074 1662 1408 686 552 389
SwaI ATTT'AAAT
Cuts at: 4320 4320
Size: 7771
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4027 4956 5068 5798 5815 5968 6153
Size: 1255 2447 929 112 730 17 153 185
Cuts at: 6153 6598 325
Size: 445 1498
Fragments arranged by size:
2447 1498 1255 929 730 445 185 153
112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3194 6017
Size: 274 80 33 51 1443 30 1187 2823
Cuts at: 6017 7729 96
Size: 1712 138
Fragments arranged by size:
2823 1712 1443 1187 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 3100 3132 7731 7763 2405
Size: 32 663 32 4599 32 2413
Fragments arranged by size:
4599 2413 663 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4038 1316
Size: 2722 5049
XbaI T'CTAG_A
Cuts at: 4438 5107 4438
Size: 669 7102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2926 2926
Size: 7771
XhoI C'TCGA_G
Cuts at: 1943 3386 1943
Size: 1443 6328
XmaI C'CCGG_G
Cuts at: 1870 2066 3119 5096 1870
Size: 196 1053 1977 4545
Fragments arranged by size:
4545 1977 1053 196
XmnI GAAnn'nnTTC
Cuts at: 2208 2498 2774 3978 4805 4838 5013 5900 2208
Size: 290 276 1204 827 33 175 887 4079
Fragments arranged by size:
4079 1204 887 827 290 276 175 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5579 276
Size: 53 83 186 936 4045 2468
Fragments arranged by size:
4045 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SrfI