Restriction map for
pFN21AE4244
(Circular) (Six-base) MAPSORT of: pFN21AE4244.seq Check: 7103 from: 1 to: 7432
pFN21AE4244 7432 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5242 278
Size: 53 83 186 936 3706 2468
Fragments arranged by size:
3706 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2644 4775 1937
Size: 707 2131 4594
Fragments arranged by size:
4594 2131 707
Acc65I G'GTAC_C
Cuts at: 4753 4753
Size: 7432
AclI AA'CG_TT
Cuts at: 1563 5559 5932 1563
Size: 3996 373 3063
Fragments arranged by size:
3996 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2160 2937 4437 5495 6879 7147 1632
Size: 423 105 777 1500 1058 1384 268 1917
Fragments arranged by size:
1917 1500 1384 1058 777 423 268 105
AfeI AGC'GCT
Cuts at: 3194 3194
Size: 7432
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7235
AflIII A'CryG_T
Cuts at: 1203 6331 7308 1203
Size: 5128 977 1327
Fragments arranged by size:
5128 1327 977
AgeI A'CCGG_T
Cuts at: 6263 6263
Size: 7432
AhdI GACnn_n'nnGTC
Cuts at: 6161 6161
Size: 7432
AleI CACnn'nnGTG
Cuts at: 1166 3144 3156 1166
Size: 1978 12 5442
Fragments arranged by size:
5442 1978 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4209 1184
Size: 3025 4407
AlwNI CAG_nnn'CTG
Cuts at: 5175 6747 5175
Size: 1572 5860
ApaLI G'TGCA_C
Cuts at: 2304 5489 6645 2304
Size: 3185 1156 3091
Fragments arranged by size:
3185 3091 1156
ApoI r'AATT_y
Cuts at: 1493 2218 2297 2426 2444 2492 2633 2854 3251
Size: 725 79 129 18 48 141 221 397
Cuts at: 3251 3356 3598 4029 4101 4741 4967 5119 7228
Size: 105 242 431 72 640 226 152 2109
Cuts at: 7228 1493
Size: 1697
Fragments arranged by size:
2109 1697 725 640 431 397 242 226
221 152 141 129 105 79 72 48
18
AseI AT'TA_AT
Cuts at: 160 1032 5986 160
Size: 872 4954 1606
Fragments arranged by size:
4954 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7432
AvaI C'yCGr_G
Cuts at: 1870 1943 4757 1870
Size: 73 2814 4545
Fragments arranged by size:
4545 2814 73
BamHI G'GATC_C
Cuts at: 4762 5129 4762
Size: 367 7065
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4753 6208 618
Size: 325 217 627 2966 1455 1842
Fragments arranged by size:
2966 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4751 1391
Size: 3360 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2614 3314 3380 3433 928
Size: 971 235 480 700 66 53 4927
Fragments arranged by size:
4927 971 700 480 235 66 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2522 2556 3737 3771 5623 5657 1903
Size: 34 585 34 1181 34 1852 34 3678
Fragments arranged by size:
3678 1852 1181 585 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4306 5324 6534 4306
Size: 1018 1210 5204
Fragments arranged by size:
5204 1210 1018
BclI T'GATC_A
Cuts at: 1548 3850 1548
Size: 2302 5130
BfrBI ATG'CAT
Cuts at: 4060 4060
Size: 7432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6043 136
Size: 107 122 71 631 605 4371 1525
Fragments arranged by size:
4371 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7427 7427
Size: 7432
BlpI GC'TnA_GC
Cuts at: 4849 4849
Size: 7432
Bme1580I G_kGCm'C
Cuts at: 1790 2308 2783 3278 5493 6649 1790
Size: 518 475 495 2215 1156 2573
Fragments arranged by size:
2573 2215 1156 518 495 475
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7432
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4613 4685 5163
Size: 416 53 83 399 24 3196 72 478
Cuts at: 5163 6121 442
Size: 958 1753
Fragments arranged by size:
3196 1753 958 478 416 399 83 72
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 2756 3036 6092 1239
Size: 921 596 280 3056 2579
Fragments arranged by size:
3056 2579 921 596 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 3447 5557 6422 6720 2057
Size: 1390 2110 865 298 2769
Fragments arranged by size:
2769 2110 1390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6095 882
Size: 617 4596 2219
Fragments arranged by size:
4596 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2395 5223 493
Size: 711 1191 2828 2702
Fragments arranged by size:
2828 2702 1191 711
BsaBI GATnn'nnATC
Cuts at: 7426 7426
Size: 7432
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5239 5621 6256 275
Size: 53 83 186 936 3706 382 635 1451
Fragments arranged by size:
3706 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5865 6263 6537 6684 7095 5865
Size: 398 274 147 411 6202
Fragments arranged by size:
6202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2927 2957 3257 3287 3963
Size: 30 165 30 1524 30 300 30 676
Cuts at: 3963 3993 4576 4606 1178
Size: 30 583 30 4004
Fragments arranged by size:
4004 1524 676 583 300 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2948 2951 4584 1186
Size: 843 919 3 1633 4034
Fragments arranged by size:
4034 1633 919 843 3
BseYI C'CCAG_C
Cuts at: 6635 7131 6635
Size: 496 6936
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6859
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4882 5643 6671 1540
Size: 275 186 2881 761 1028 2301
Fragments arranged by size:
2881 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2308 3270 5493 5578 6649 2308
Size: 962 2223 85 1071 3091
Fragments arranged by size:
3091 2223 1071 962 85
BsmI GAATG_Cn'
Cuts at: 4072 4943 5036 4072
Size: 871 93 6468
Fragments arranged by size:
6468 871 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2308 2783 3270 3278 4751 5493 5578
Size: 399 518 475 487 8 1473 742 85
Cuts at: 5578 6649 1391
Size: 1071 2174
Fragments arranged by size:
2174 1473 1071 742 518 487 475 399
85 8
BspEI T'CCGG_A
Cuts at: 7095 7095
Size: 7432
BspHI T'CATG_A
Cuts at: 3037 3796 3847 4243 4510 5321 7051 3037
Size: 759 51 396 267 811 1730 3418
Fragments arranged by size:
3418 1730 811 759 396 267 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4609 4787 844
Size: 3765 178 3489
Fragments arranged by size:
3765 3489 178
BsrBI CCG'CTC
Cuts at: 1394 1823 5319 1394
Size: 429 3496 3507
Fragments arranged by size:
3507 3496 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5927 6101 65
Size: 1183 4679 174 1396
Fragments arranged by size:
4679 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6076 6263 1700
Size: 110 4266 187 2869
Fragments arranged by size:
4266 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2454 96
Size: 2358 5074
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7432
BssSI C'ACGA_G
Cuts at: 4551 5492 6504 4551
Size: 941 1012 5479
Fragments arranged by size:
5479 1012 941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7432
BstBI TT'CG_AA
Cuts at: 4066 6236 4066
Size: 2170 5262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7432
BstYI r'GATC_y
Cuts at: 1972 2043 4642 4762 5129 5514 5531 6983 7069
Size: 71 2599 120 367 385 17 1452 86
Cuts at: 7069 7081 7427 1972
Size: 12 346 1977
Fragments arranged by size:
2599 1977 1452 385 367 346 120 86
71 17 12
Bsu36I CC'TnA_GG
Cuts at: 3009 3009
Size: 7432
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6880
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2820 3834 487
Size: 683 56 1594 1014 4085
Fragments arranged by size:
4085 1594 1014 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 2520 3095 4948 5742 5762 2094
Size: 426 575 1853 794 20 3764
Fragments arranged by size:
3764 1853 794 575 426 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7432
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7397
DraI TTT'AAA
Cuts at: 2235 3505 4036 4736 2235
Size: 1270 531 700 4931
Fragments arranged by size:
4931 1270 700 531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6439 809
Size: 507 5123 1802
Fragments arranged by size:
5123 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4879 5768 7397
Size: 54 1425 50 275 18 3049 889 1629
Cuts at: 7397 8
Size: 43
Fragments arranged by size:
3049 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4879 1537
Size: 275 3067 4090
Fragments arranged by size:
4090 3067 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2151 5362 1353
Size: 653 145 3211 3423
Fragments arranged by size:
3423 3211 653 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6019 6393 6539 387
Size: 1064 138 4430 374 146 1280
Fragments arranged by size:
4430 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2160 2756 2937 3036 4437 5495
Size: 393 423 105 596 181 99 1401 1058
Cuts at: 5495 6092 6879 7147 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1401 1058 787 597 596 423 393
268 181 105 99
EcoO109I rG'GnC_Cy
Cuts at: 2697 2893 3551 2697
Size: 196 658 6578
Fragments arranged by size:
6578 658 196
EcoRI G'AATT_C
Cuts at: 4741 5119 4741
Size: 378 7054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3702 3702
Size: 7432
FspI TGC'GCA
Cuts at: 5938 5938
Size: 7432
HaeII r_GCGC'y
Cuts at: 1678 3196 6579 1678
Size: 1518 3383 2531
Fragments arranged by size:
3383 2531 1518
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 2347 2379 2927
Size: 32 135 32 526 32 217 32 548
Cuts at: 2927 2939 2959 2971 3322 3354 4301 4333 4505
Size: 12 20 12 351 32 947 32 172
Cuts at: 4505 4537 6073 6105 6147 6179 1373
Size: 32 1536 32 42 32 2626
Fragments arranged by size:
2626 1536 947 548 526 351 217 172
135 42 32 32 32 32 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 4539 4776 5022 669
Size: 988 281 1110 1491 237 246 3079
Fragments arranged by size:
3079 1491 1110 988 281 246 237
HindIII A'AGCT_T
Cuts at: 748 2189 748
Size: 1441 5991
HpaI GTT'AAC
Cuts at: 3048 5022 3048
Size: 1974 5458
KpnI G_GTAC'C
Cuts at: 4757 4757
Size: 7432
MfeI C'AATT_G
Cuts at: 5031 5031
Size: 7432
MluI A'CGCG_T
Cuts at: 7308 7308
Size: 7432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3371 4362 6546 6730 1305
Size: 2066 991 2184 184 2007
Fragments arranged by size:
2184 2066 2007 991 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5610
Fragments arranged by size:
5610 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2991 3144 3156 3240 3798 3882 3900
Size: 648 1825 153 12 84 558 84 18
Cuts at: 3900 4011 4077 4093 4161 4191 4242 5390 5749
Size: 111 66 16 68 30 51 1148 359
Cuts at: 5749 5908 518
Size: 159 2042
Fragments arranged by size:
2042 1825 1148 648 558 359 159 153
111 84 84 68 66 51 30 18
16 12
MspA1I CmG'CkG
Cuts at: 1606 4559 4848 5525 6673 6918 1606
Size: 2953 289 677 1148 245 2120
Fragments arranged by size:
2953 2120 1148 677 289 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6880
NdeI CA'TA_TG
Cuts at: 387 3447 387
Size: 3060 4372
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7432
NotI GC'GGCC_GC
Cuts at: 4879 4879
Size: 7432
NsiI A_TGCA'T
Cuts at: 4062 4062
Size: 7432
NspI r_CATG'y
Cuts at: 1132 1229 3326 4790 6335 1132
Size: 97 2097 1464 1545 2229
Fragments arranged by size:
2229 2097 1545 1464 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7432
PciI A'CATG_T
Cuts at: 6331 6331
Size: 7432
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7432
PmeI GTTT'AAAC
Cuts at: 4736 4736
Size: 7432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5501 5533 7014 7046 5501
Size: 32 1481 32 5887
Fragments arranged by size:
5887 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2697 2893 3551 2697
Size: 196 658 6578
Fragments arranged by size:
6578 658 196
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7432
PsiI TTA'TAA
Cuts at: 2658 5002 2658
Size: 2344 5088
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3018 3050 4425 4457 3018
Size: 32 1375 32 5993
Fragments arranged by size:
5993 1375 32 32
PstI C_TGCA'G
Cuts at: 830 2125 4784 830
Size: 1295 2659 3478
Fragments arranged by size:
3478 2659 1295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7432
SalI G'TCGA_C
Cuts at: 1936 4774 1936
Size: 2838 4594
SapI GCTCTTCn'nnn_
Cuts at: 2151 2151
Size: 7432
SbfI CC_TGCA'GG
Cuts at: 4784 4784
Size: 7432
ScaI AGT'ACT
Cuts at: 2954 4276 4313 5680 7392 2954
Size: 1322 37 1367 1712 2994
Fragments arranged by size:
2994 1712 1367 1322 37
SexAI A'CCwGG_T
Cuts at: 4146 4146
Size: 7432
SfcI C'TryA_G
Cuts at: 826 1045 2121 2638 3024 4780 5915 6596 6787
Size: 219 1076 517 386 1756 1135 681 191
Cuts at: 6787 826
Size: 1471
Fragments arranged by size:
1756 1471 1135 1076 681 517 386 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7432
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7432
SmaI CCC'GGG
Cuts at: 1872 4759 1872
Size: 2887 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 3462 5536 6437 6699 820
Size: 197 926 93 1426 2074 901 262 1553
Fragments arranged by size:
2074 1553 1426 926 901 262 197 93
SnaBI TAC'GTA
Cuts at: 493 5223 493
Size: 4730 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7432
SphI G_CATG'C
Cuts at: 1132 4790 1132
Size: 3658 3774
SspI AAT'ATT
Cuts at: 5 52 2231 3084 4708 5356 5
Size: 47 2179 853 1624 648 2081
Fragments arranged by size:
2179 2081 1624 853 648 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7432
StyI C'CwwG_G
Cuts at: 513 1065 3967 4871 513
Size: 552 2902 904 3074
Fragments arranged by size:
3074 2902 904 552
SwaI ATTT'AAAT
Cuts at: 2235 2235
Size: 7432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3132 4632 5459 5476 5629 5814 6259
Size: 1255 1552 1500 827 17 153 185 445
Cuts at: 6259 325
Size: 1498
Fragments arranged by size:
1552 1500 1498 1255 827 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2454 2952 3620
Size: 274 80 33 51 1443 477 498 668
Cuts at: 3620 3790 4274 4311 5678 7390 96
Size: 170 484 37 1367 1712 138
Fragments arranged by size:
1712 1443 1367 668 498 484 477 274
170 138 80 51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3963 3995 7392 7424 3257
Size: 32 674 32 3397 32 3265
Fragments arranged by size:
3397 3265 674 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7432
XbaI T'CTAG_A
Cuts at: 4768 4768
Size: 7432
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7432
XmaI C'CCGG_G
Cuts at: 1870 4757 1870
Size: 2887 4545
XmnI GAAnn'nnTTC
Cuts at: 2145 2884 4072 5561 2145
Size: 739 1188 1489 4016
Fragments arranged by size:
4016 1489 1188 739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5240 276
Size: 53 83 186 936 3706 2468
Fragments arranged by size:
3706 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SfiI SfoI SrfI XcmI