Restriction map for
pFN21AE4242
(Circular) (Six-base) MAPSORT of: pFN21AE4242.seq Check: 7333 from: 1 to: 7417
pFN21AE4242 7417 bp
With 182 enzymes: SgfI *
March 28, 2012 13:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5227 278
Size: 53 83 186 936 3691 2468
Fragments arranged by size:
3691 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4760 1937
Size: 2823 4594
Acc65I G'GTAC_C
Cuts at: 4738 4738
Size: 7417
AclI AA'CG_TT
Cuts at: 1563 5544 5917 1563
Size: 3981 373 3063
Fragments arranged by size:
3981 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3129 3174 4088 4598 5480 6864 7132 1632
Size: 1497 45 914 510 882 1384 268 1917
Fragments arranged by size:
1917 1497 1384 914 882 510 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7220
AflIII A'CryG_T
Cuts at: 1203 6316 7293 1203
Size: 5113 977 1327
Fragments arranged by size:
5113 1327 977
AgeI A'CCGG_T
Cuts at: 6248 6248
Size: 7417
AhdI GACnn_n'nnGTC
Cuts at: 3619 6146 3619
Size: 2527 4890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7417
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3201 3233 4649 4681 3201
Size: 32 1416 32 5937
Fragments arranged by size:
5937 1416 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3757 5160 6732 3757
Size: 1403 1572 4442
Fragments arranged by size:
4442 1572 1403
ApaI G_GGCC'C
Cuts at: 3929 3929
Size: 7417
ApaLI G'TGCA_C
Cuts at: 5474 6630 5474
Size: 1156 6261
ApoI r'AATT_y
Cuts at: 1493 2138 2571 3094 3646 4726 4952 5104 7213
Size: 645 433 523 552 1080 226 152 2109
Cuts at: 7213 1493
Size: 1697
Fragments arranged by size:
2109 1697 1080 645 552 523 433 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3663 5971 160
Size: 872 2631 2308 1606
Fragments arranged by size:
2631 2308 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
AvaI C'yCGr_G
Cuts at: 1870 1943 4742 1870
Size: 73 2799 4545
Fragments arranged by size:
4545 2799 73
AvrII C'CTAG_G
Cuts at: 2474 2474
Size: 7417
BamHI G'GATC_C
Cuts at: 3314 4747 5114 3314
Size: 1433 367 5617
Fragments arranged by size:
5617 1433 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4738 6193 618
Size: 325 217 627 2951 1455 1842
Fragments arranged by size:
2951 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3929 4736 1391
Size: 2538 807 4072
Fragments arranged by size:
4072 2538 807
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3606 928
Size: 971 1707 4739
Fragments arranged by size:
4739 1707 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5608 5642 1903
Size: 34 3671 34 3678
Fragments arranged by size:
3678 3671 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3441 5309 6519 3441
Size: 1868 1210 4339
Fragments arranged by size:
4339 1868 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7417
BfrBI ATG'CAT
Cuts at: 4604 4604
Size: 7417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6028 136
Size: 107 122 71 631 605 4356 1525
Fragments arranged by size:
4356 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2846 7412 2846
Size: 4566 2851
BlpI GC'TnA_GC
Cuts at: 3629 4103 4834 3629
Size: 474 731 6212
Fragments arranged by size:
6212 731 474
Bme1580I G_kGCm'C
Cuts at: 1790 3189 3929 4490 5478 6634 1790
Size: 1399 740 561 988 1156 2573
Fragments arranged by size:
2573 1399 1156 988 740 561
BmgBI CAC'GTC
Cuts at: 1318 3372 1318
Size: 2054 5363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3276 5148 6106
Size: 416 53 83 399 24 1859 1872 958
Cuts at: 6106 442
Size: 1753
Fragments arranged by size:
1872 1859 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2558 3665 3689 6077 1239
Size: 1319 1107 24 2388 2579
Fragments arranged by size:
2579 2388 1319 1107 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5542 6407 6705 5542
Size: 865 298 6254
Fragments arranged by size:
6254 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6080 882
Size: 617 4581 2219
Fragments arranged by size:
4581 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2594 3172 3748 5208 493
Size: 711 1390 578 576 1460 2702
Fragments arranged by size:
2702 1460 1390 711 578 576
BsaBI GATnn'nnATC
Cuts at: 7411 7411
Size: 7417
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5224 5606 6241 275
Size: 53 83 186 936 3691 382 635 1451
Fragments arranged by size:
3691 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5850 6248 6522 6669 7080 5850
Size: 398 274 147 411 6187
Fragments arranged by size:
6187 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3936 3966 1178
Size: 30 165 30 2533 30 4629
Fragments arranged by size:
4629 2533 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7417
BseYI C'CCAG_C
Cuts at: 3225 3310 3928 6620 7116 3225
Size: 85 618 2692 496 3526
Fragments arranged by size:
3526 2692 618 496 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3770 4054 1163
Size: 573 2034 284 4526
Fragments arranged by size:
4526 2034 573 284
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4867 5628 6656 1540
Size: 275 186 2866 761 1028 2301
Fragments arranged by size:
2866 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5478 5563 6634 5478
Size: 85 1071 6261
Fragments arranged by size:
6261 1071 85
BsmI GAATG_Cn'
Cuts at: 4052 4606 4928 5021 4052
Size: 554 322 93 6448
Fragments arranged by size:
6448 554 322 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3189 3929 4490 4736 5478 5563 6634
Size: 399 1399 740 561 246 742 85 1071
Cuts at: 6634 1391
Size: 2174
Fragments arranged by size:
2174 1399 1071 742 740 561 399 246
85
BspEI T'CCGG_A
Cuts at: 7080 7080
Size: 7417
BspHI T'CATG_A
Cuts at: 5306 7036 5306
Size: 1730 5687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4772 844
Size: 3928 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5304 1394
Size: 429 3481 3507
Fragments arranged by size:
3507 3481 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 4384 4536 5912 6086 65
Size: 1183 1191 1945 152 1376 174 1396
Fragments arranged by size:
1945 1396 1376 1191 1183 174 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 6061 6248 1700
Size: 110 4251 187 2869
Fragments arranged by size:
4251 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7417
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7417
BssSI C'ACGA_G
Cuts at: 5477 6489 5477
Size: 1012 6405
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7417
BstBI TT'CG_AA
Cuts at: 4687 6221 4687
Size: 1534 5883
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7417
BstYI r'GATC_y
Cuts at: 1972 2846 3314 4747 5114 5499 5516 6968 7054
Size: 874 468 1433 367 385 17 1452 86
Cuts at: 7054 7066 7412 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1433 874 468 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2561 2561
Size: 7417
BtgI C'CryG_G
Cuts at: 513 1065 2004 3498 4204 513
Size: 552 939 1494 706 3726
Fragments arranged by size:
3726 1494 939 706 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3054 487
Size: 683 56 1828 4850
Fragments arranged by size:
4850 1828 683 56
BtsI GCAGTG_nn'
Cuts at: 4218 4933 5727 5747 4218
Size: 715 794 20 5888
Fragments arranged by size:
5888 794 715 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3253 3288 4207 4242 529
Size: 35 2689 35 919 35 3704
Fragments arranged by size:
3704 2689 919 35 35 35
DraI TTT'AAA
Cuts at: 3645 4721 3645
Size: 1076 6341
DraIII CAC_nnn'GTG
Cuts at: 3940 3940
Size: 7417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6424 809
Size: 507 5108 1802
Fragments arranged by size:
5108 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2864 3147
Size: 54 1425 50 275 18 390 644 283
Cuts at: 3147 4864 5753 7382 8
Size: 1717 889 1629 43
Fragments arranged by size:
1717 1629 1425 889 644 390 283 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4864 1537
Size: 275 3052 4090
Fragments arranged by size:
4090 3052 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3343 3449 4064 4460 5347 1353
Size: 1990 106 615 396 887 3423
Fragments arranged by size:
3423 1990 887 615 396 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6004 6378 6524 387
Size: 1064 138 4415 374 146 1280
Fragments arranged by size:
4415 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2558 3129 3174 3665 3689 4088 4598
Size: 393 926 571 45 491 24 399 510
Cuts at: 4598 5480 6077 6864 7132 1239
Size: 882 597 787 268 1524
Fragments arranged by size:
1524 926 882 787 597 571 510 491
399 393 268 45 24
EcoO109I rG'GnC_Cy
Cuts at: 3331 3925 3331
Size: 594 6823
EcoRI G'AATT_C
Cuts at: 2138 4726 5104 2138
Size: 2588 378 4451
Fragments arranged by size:
4451 2588 378
EcoRV GAT'ATC
Cuts at: 4352 4352
Size: 7417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4169 4428 4169
Size: 259 7158
FspI TGC'GCA
Cuts at: 3537 5923 3537
Size: 2386 5031
HaeII r_GCGC'y
Cuts at: 1678 2434 6564 1678
Size: 756 4130 2531
Fragments arranged by size:
4130 2531 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2831 2863 3605 3637 3859
Size: 32 135 32 1259 32 742 32 222
Cuts at: 3859 3891 3936 3968 4280 4312 6058 6090 6132
Size: 32 45 32 312 32 1746 32 42
Cuts at: 6132 6164 1373
Size: 32 2626
Fragments arranged by size:
2626 1746 1259 742 312 222 135 45
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4201 4761 5007 669
Size: 988 281 2263 560 246 3079
Fragments arranged by size:
3079 2263 988 560 281 246
HindIII A'AGCT_T
Cuts at: 748 2718 748
Size: 1970 5447
HpaI GTT'AAC
Cuts at: 5007 5007
Size: 7417
KpnI G_GTAC'C
Cuts at: 4742 4742
Size: 7417
MfeI C'AATT_G
Cuts at: 4224 5016 4224
Size: 792 6625
MluI A'CGCG_T
Cuts at: 7293 7293
Size: 7417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2426 6531 6715 1305
Size: 1121 4105 184 2007
Fragments arranged by size:
4105 2007 1121 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 3149 10
Size: 54 1425 343 390 927 4278
Fragments arranged by size:
4278 1425 927 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2835 3503 4280 4389 4434 5375 5734
Size: 648 1669 668 777 109 45 941 359
Cuts at: 5734 5893 518
Size: 159 2042
Fragments arranged by size:
2042 1669 941 777 668 648 359 159
109 45
MspA1I CmG'CkG
Cuts at: 1606 2306 2319 3229 3427 4833 5510 6658 6903
Size: 700 13 910 198 1406 677 1148 245
Cuts at: 6903 1606
Size: 2120
Fragments arranged by size:
2120 1406 1148 910 700 677 245 198
13
NcoI C'CATG_G
Cuts at: 513 1065 2004 3498 513
Size: 552 939 1494 4432
Fragments arranged by size:
4432 1494 939 552
NdeI CA'TA_TG
Cuts at: 387 2854 387
Size: 2467 4950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7417
NotI GC'GGCC_GC
Cuts at: 4864 4864
Size: 7417
NsiI A_TGCA'T
Cuts at: 4606 4606
Size: 7417
NspI r_CATG'y
Cuts at: 1132 1229 4199 4775 6320 1132
Size: 97 2970 576 1545 2229
Fragments arranged by size:
2970 2229 1545 576 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7417
PciI A'CATG_T
Cuts at: 6316 6316
Size: 7417
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7417
PfoI T'CCnGG_A
Cuts at: 2888 2888
Size: 7417
PmeI GTTT'AAAC
Cuts at: 4721 4721
Size: 7417
PmlI CAC'GTG
Cuts at: 3172 3172
Size: 7417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5486 5518 6999 7031 5486
Size: 32 1481 32 5872
Fragments arranged by size:
5872 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7417
PsiI TTA'TAA
Cuts at: 2096 4987 2096
Size: 2891 4526
PspOMI G'GGCC_C
Cuts at: 3925 3925
Size: 7417
PstI C_TGCA'G
Cuts at: 830 3453 3918 4192 4769 830
Size: 2623 465 274 577 3478
Fragments arranged by size:
3478 2623 577 465 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7417
PvuII CAG'CTG
Cuts at: 2319 3229 3427 2319
Size: 910 198 6309
Fragments arranged by size:
6309 910 198
SalI G'TCGA_C
Cuts at: 1936 4759 1936
Size: 2823 4594
SapI GCTCTTCn'nnn_
Cuts at: 3449 3449
Size: 7417
SbfI CC_TGCA'GG
Cuts at: 4769 4769
Size: 7417
ScaI AGT'ACT
Cuts at: 4683 5665 7377 4683
Size: 982 1712 4723
Fragments arranged by size:
4723 1712 982
SfcI C'TryA_G
Cuts at: 826 1045 2238 3365 3449 3863 3914 4188 4644
Size: 219 1193 1127 84 414 51 274 456
Cuts at: 4644 4765 5900 6581 6772 826
Size: 121 1135 681 191 1471
Fragments arranged by size:
1471 1193 1135 1127 681 456 414 274
219 191 121 84 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7417
SmaI CCC'GGG
Cuts at: 1872 4744 1872
Size: 2872 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5521 6422 6684 820
Size: 197 926 3578 901 262 1553
Fragments arranged by size:
3578 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2594 5208 493
Size: 2101 2614 2702
Fragments arranged by size:
2702 2614 2101
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7417
SphI G_CATG'C
Cuts at: 1132 4775 1132
Size: 3643 3774
SspI AAT'ATT
Cuts at: 5 52 2776 3033 4576 5341 5
Size: 47 2724 257 1543 765 2081
Fragments arranged by size:
2724 2081 1543 765 257 47
StuI AGG'CCT
Cuts at: 1517 3106 1517
Size: 1589 5828
StyI C'CwwG_G
Cuts at: 513 1065 2004 2474 2624 2967 3070 3190 3498
Size: 552 939 470 150 343 103 120 308
Cuts at: 3498 4856 513
Size: 1358 3074
Fragments arranged by size:
3074 1358 939 552 470 343 308 150
120 103
SwaI ATTT'AAAT
Cuts at: 3645 3645
Size: 7417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 5444 5461 5614 5799 6244 325
Size: 1255 1037 2827 17 153 185 445 1498
Fragments arranged by size:
2827 1498 1255 1037 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4681 5663 7375
Size: 274 80 33 51 1443 2704 982 1712
Cuts at: 7375 96
Size: 138
Fragments arranged by size:
2704 1712 1443 982 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3129 3161 7377 7409 3129
Size: 32 4216 32 3137
Fragments arranged by size:
4216 3137 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3373 1316
Size: 2057 5360
XbaI T'CTAG_A
Cuts at: 3892 4753 3892
Size: 861 6556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4629 4629
Size: 7417
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7417
XmaI C'CCGG_G
Cuts at: 1870 4742 1870
Size: 2872 4545
XmnI GAAnn'nnTTC
Cuts at: 2788 2976 4575 5546 2788
Size: 188 1599 971 4659
Fragments arranged by size:
4659 1599 971 188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5225 276
Size: 53 83 186 936 3691 2468
Fragments arranged by size:
3691 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI