Restriction map for
pFN21AE4240
(Circular) (Six-base) MAPSORT of: pFN21AE4240.seq Check: 4161 from: 1 to: 7330
pFN21AE4240 7330 bp
With 182 enzymes: SgfI *
March 28, 2012 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2722 3734 3830 2722
Size: 1012 96 6222
Fragments arranged by size:
6222 1012 96
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3156 5140 278
Size: 53 83 186 936 1620 1984 2468
Fragments arranged by size:
2468 1984 1620 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 4044 4673 1937
Size: 1243 864 629 4594
Fragments arranged by size:
4594 1243 864 629
Acc65I G'GTAC_C
Cuts at: 3849 4651 3849
Size: 802 6528
AclI AA'CG_TT
Cuts at: 1563 5457 5830 1563
Size: 3894 373 3063
Fragments arranged by size:
3894 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2503 3874 5393 6777 7045 1632
Size: 871 1371 1519 1384 268 1917
Fragments arranged by size:
1917 1519 1384 1371 871 268
AfeI AGC'GCT
Cuts at: 3803 3803
Size: 7330
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7133
AflIII A'CryG_T
Cuts at: 1203 3972 4203 4272 6229 7206 1203
Size: 2769 231 69 1957 977 1327
Fragments arranged by size:
2769 1957 1327 977 231 69
AgeI A'CCGG_T
Cuts at: 6161 6161
Size: 7330
AhdI GACnn_n'nnGTC
Cuts at: 2317 6059 2317
Size: 3742 3588
AleI CACnn'nnGTG
Cuts at: 1166 2756 1166
Size: 1590 5740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7330
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3986 4018 3986
Size: 32 7298
AlwNI CAG_nnn'CTG
Cuts at: 2100 2213 2329 2644 2680 2701 2890 3309 5073
Size: 113 116 315 36 21 189 419 1764
Cuts at: 5073 6645 2100
Size: 1572 2785
Fragments arranged by size:
2785 1764 1572 419 315 189 116 113
36 21
ApaI G_GGCC'C
Cuts at: 2589 2784 2856 4418 4509 2589
Size: 195 72 1562 91 5410
Fragments arranged by size:
5410 1562 195 91 72
ApaLI G'TGCA_C
Cuts at: 5387 6543 5387
Size: 1156 6174
ApoI r'AATT_y
Cuts at: 1493 4639 4865 5017 7126 1493
Size: 3146 226 152 2109 1697
Fragments arranged by size:
3146 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5884 160
Size: 872 4852 1606
Fragments arranged by size:
4852 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2929 3284 3395 4067 4655 1870
Size: 73 170 816 355 111 672 588 4545
Fragments arranged by size:
4545 816 672 588 355 170 111 73
BamHI G'GATC_C
Cuts at: 4660 5027 4660
Size: 367 6963
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 2179 2834 2934 3849
Size: 325 217 627 226 166 655 100 915
Cuts at: 3849 4651 6106 618
Size: 802 1455 1842
Fragments arranged by size:
1842 1455 915 802 655 627 325 226
217 166 100
BanII G_rGCy'C
Cuts at: 1391 2589 2784 2856 3129 3191 3357 3561 4418
Size: 1198 195 72 273 62 166 204 857
Cuts at: 4418 4509 4539 4649 1391
Size: 91 30 110 4072
Fragments arranged by size:
4072 1198 857 273 204 195 166 110
91 72 62 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6359
BbvCI CC'TCA_GC
Cuts at: 2144 2625 2694 3422 3483 3753 2144
Size: 481 69 728 61 270 5721
Fragments arranged by size:
5721 728 481 270 69 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5521 5555 1903
Size: 34 3584 34 3678
Fragments arranged by size:
3678 3584 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5222 6432 5222
Size: 1210 6120
BclI T'GATC_A
Cuts at: 1548 3630 1548
Size: 2082 5248
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2188 3108 5941
Size: 107 122 71 631 605 516 920 2833
Cuts at: 5941 136
Size: 1525
Fragments arranged by size:
2833 1525 920 631 605 516 122 107
71
BglII A'GATC_T
Cuts at: 3387 4197 7325 3387
Size: 810 3128 3392
Fragments arranged by size:
3392 3128 810
BlpI GC'TnA_GC
Cuts at: 3555 4747 3555
Size: 1192 6138
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2589 2784 2839 2856 2937 4418 4509
Size: 394 405 195 55 17 81 1481 91
Cuts at: 4509 5391 6547 1790
Size: 882 1156 2573
Fragments arranged by size:
2573 1481 1156 882 405 394 195 91
81 55 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7330
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2823 5061 6019
Size: 416 53 83 399 24 1406 2238 958
Cuts at: 6019 442
Size: 1753
Fragments arranged by size:
2238 1753 1406 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7330
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 7330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3143 3179 3191 3248 3988 4232 4503 5990
Size: 1904 36 12 57 740 244 271 1487
Cuts at: 5990 1239
Size: 2579
Fragments arranged by size:
2579 1904 1487 740 271 244 57 36
12
Bpu10I CC'TnA_GC
Cuts at: 2144 2625 2694 3422 3483 3753 2144
Size: 481 69 728 61 270 5721
Fragments arranged by size:
5721 728 481 270 69 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5455 6320 6618 5455
Size: 865 298 6167
Fragments arranged by size:
6167 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5993 882
Size: 617 4494 2219
Fragments arranged by size:
4494 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2581 4102 4204 4275 5121 493
Size: 711 1377 1521 102 71 846 2702
Fragments arranged by size:
2702 1521 1377 846 711 102 71
BsaBI GATnn'nnATC
Cuts at: 7324 7324
Size: 7330
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2357 3153 3716 4242
Size: 53 83 186 936 824 796 563 526
Cuts at: 4242 5137 5519 6154 275
Size: 895 382 635 1451
Fragments arranged by size:
1451 936 895 824 796 635 563 526
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2343 5763 6161 6435 6582 6993 2343
Size: 3420 398 274 147 411 2680
Fragments arranged by size:
3420 2680 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2275 2305 3173 3203 3479
Size: 30 165 30 872 30 868 30 276
Cuts at: 3479 3509 3986 4016 4476 4506 1178
Size: 30 477 30 460 30 4002
Fragments arranged by size:
4002 872 868 477 460 276 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3428 3500 4550 1186
Size: 2242 72 1050 3966
Fragments arranged by size:
3966 2242 1050 72
BseYI C'CCAG_C
Cuts at: 2049 2505 2589 2808 3593 3798 4513 6533 7029
Size: 456 84 219 785 205 715 2020 496
Cuts at: 7029 2049
Size: 2350
Fragments arranged by size:
2350 2020 785 715 496 456 219 205
84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2206 3367 3924 1163
Size: 573 470 1161 557 4569
Fragments arranged by size:
4569 1161 573 557 470
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3731 4780 5541 6569 1540
Size: 275 186 1111 619 1049 761 1028 2301
Fragments arranged by size:
2301 1111 1049 1028 761 619 275 186
BsiHKAI G_wGCw'C
Cuts at: 3129 3357 3561 5391 5476 6547 3129
Size: 228 204 1830 85 1071 3912
Fragments arranged by size:
3912 1830 1071 228 204 85
BsmI GAATG_Cn'
Cuts at: 4841 4934 4841
Size: 93 7237
BsmBI CGTCTCn'nnnn_
Cuts at: 3460 4006 3460
Size: 546 6784
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2589 2784 2839 2856 2937 3129
Size: 399 394 405 195 55 17 81 192
Cuts at: 3129 3191 3357 3561 4418 4509 4539 4649 5391
Size: 62 166 204 857 91 30 110 742
Cuts at: 5391 5476 6547 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 857 742 405 399 394 204
195 192 166 110 91 85 81 62
55 30 17
BspEI T'CCGG_A
Cuts at: 6993 6993
Size: 7330
BspHI T'CATG_A
Cuts at: 5219 6949 5219
Size: 1730 5600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2722 3150 3609 3734 3830 4685 844
Size: 1878 428 459 125 96 855 3489
Fragments arranged by size:
3489 1878 855 459 428 125 96
BsrBI CCG'CTC
Cuts at: 1394 1823 5217 1394
Size: 429 3394 3507
Fragments arranged by size:
3507 3394 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5825 5999 65
Size: 1183 4577 174 1396
Fragments arranged by size:
4577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 2228 4238 5974 6161 1700
Size: 110 310 108 2010 1736 187 2869
Fragments arranged by size:
2869 2010 1736 310 187 110 108
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7330
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7330
BssSI C'ACGA_G
Cuts at: 2255 5390 6402 2255
Size: 3135 1012 3183
Fragments arranged by size:
3183 3135 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2890 3270 794
Size: 2096 380 4854
Fragments arranged by size:
4854 2096 380
BstBI TT'CG_AA
Cuts at: 2028 6134 2028
Size: 4106 3224
BstEII G'GTnAC_C
Cuts at: 1184 2278 2446 2557 1184
Size: 1094 168 111 5957
Fragments arranged by size:
5957 1094 168 111
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 2758 2905 2257
Size: 501 147 6682
Fragments arranged by size:
6682 501 147
BstYI r'GATC_y
Cuts at: 1972 3387 3417 4197 4660 5027 5412 5429 6881
Size: 1415 30 780 463 367 385 17 1452
Cuts at: 6881 6967 6979 7325 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1415 780 463 385 367 346
86 30 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2175 2441 2451 2875 3327 3567 513
Size: 552 1110 266 10 424 452 240 4276
Fragments arranged by size:
4276 1110 552 452 424 266 240 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3430 487
Size: 683 56 2204 4387
Fragments arranged by size:
4387 2204 683 56
BtsI GCAGTG_nn'
Cuts at: 2110 2567 2714 3221 3350 4453 4504 4846 5640
Size: 457 147 507 129 1103 51 342 794
Cuts at: 5640 5660 2110
Size: 20 3780
Fragments arranged by size:
3780 1103 794 507 457 342 147 129
51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7330
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7295
DraI TTT'AAA
Cuts at: 4634 4634
Size: 7330
DraIII CAC_nnn'GTG
Cuts at: 4185 4185
Size: 7330
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6337 809
Size: 507 5021 1802
Fragments arranged by size:
5021 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2457 2703
Size: 54 1425 50 275 18 429 198 246
Cuts at: 2703 2767 3100 3109 3207 3234 3300 3324 3330
Size: 64 333 9 98 27 66 24 6
Cuts at: 3330 3366 3402 3564 3728 3813 4777 5666 7295
Size: 36 36 162 164 85 964 889 1629
Cuts at: 7295 8
Size: 43
Fragments arranged by size:
1629 1425 964 889 429 333 275 246
198 164 162 98 85 66 64 54
50 43 36 36 27 24 18 9
6
EagI C'GGCC_G
Cuts at: 1537 1812 3109 3728 4777 1537
Size: 275 1297 619 1049 4090
Fragments arranged by size:
4090 1297 1049 619 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3618 5260 1353
Size: 2265 1642 3423
Fragments arranged by size:
3423 2265 1642
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2806 3563 5917 6291 6437 387
Size: 1064 138 1217 757 2354 374 146 1280
Fragments arranged by size:
2354 1280 1217 1064 757 374 146 138
EcoICRI GAG'CTC
Cuts at: 3127 3355 3559 3127
Size: 228 204 6898
Fragments arranged by size:
6898 228 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2503 3143 3179 3191 3248 3874 3988
Size: 393 871 640 36 12 57 626 114
Cuts at: 3988 4232 4503 5393 5990 6777 7045 1239
Size: 244 271 890 597 787 268 1524
Fragments arranged by size:
1524 890 871 787 640 626 597 393
271 268 244 114 57 36 12
EcoNI CCTnn'n_nnAGG
Cuts at: 4395 4395
Size: 7330
EcoO109I rG'GnC_Cy
Cuts at: 2542 2585 2586 2677 2747 2780 2781 2853 3070
Size: 43 1 91 70 33 1 72 217
Cuts at: 3070 3526 3655 4505 2542
Size: 456 129 850 5367
Fragments arranged by size:
5367 850 456 217 129 91 72 70
43 33 1 1
EcoRI G'AATT_C
Cuts at: 4639 5017 4639
Size: 378 6952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3457 3457
Size: 7330
FspI TGC'GCA
Cuts at: 5836 5836
Size: 7330
HaeII r_GCGC'y
Cuts at: 1678 3805 6477 1678
Size: 2127 2672 2531
Fragments arranged by size:
2672 2531 2127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 2410 2442 4476
Size: 32 135 32 731 32 75 32 2034
Cuts at: 4476 4508 5971 6003 6045 6077 1373
Size: 32 1463 32 42 32 2626
Fragments arranged by size:
2626 2034 1463 731 135 75 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2074 2662 3616 4674 4920 669
Size: 988 281 136 588 954 1058 246 3079
Fragments arranged by size:
3079 1058 988 954 588 281 246 136
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7330
HpaI GTT'AAC
Cuts at: 4920 4920
Size: 7330
KpnI G_GTAC'C
Cuts at: 3853 4655 3853
Size: 802 6528
MfeI C'AATT_G
Cuts at: 4929 4929
Size: 7330
MluI A'CGCG_T
Cuts at: 7206 7206
Size: 7330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3483 6444 6628 1305
Size: 2178 2961 184 2007
Fragments arranged by size:
2961 2178 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 3326 3368 10
Size: 54 1425 343 429 1065 42 3972
Fragments arranged by size:
3972 1425 1065 429 343 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2493 2555 2756 2903 3036 5288 5647
Size: 648 1327 62 201 147 133 2252 359
Cuts at: 5647 5806 518
Size: 159 2042
Fragments arranged by size:
2252 2042 1327 648 359 201 159 147
133 62
MspA1I CmG'CkG
Cuts at: 1606 2170 2599 2756 3099 3295 4023 4322 4746
Size: 564 429 157 343 196 728 299 424
Cuts at: 4746 5423 6571 6816 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 728 677 564 429 424 343
299 245 196 157
NaeI GCC'GGC
Cuts at: 4240 4240
Size: 7330
NcoI C'CATG_G
Cuts at: 513 1065 2441 3327 513
Size: 552 1376 886 4516
Fragments arranged by size:
4516 1376 886 552
NdeI CA'TA_TG
Cuts at: 387 3684 387
Size: 3297 4033
NgoMIV G'CCGG_C
Cuts at: 4238 4238
Size: 7330
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7330
NotI GC'GGCC_GC
Cuts at: 4777 4777
Size: 7330
NspI r_CATG'y
Cuts at: 1132 1229 2668 3976 4573 4688 6233 1132
Size: 97 1439 1308 597 115 1545 2229
Fragments arranged by size:
2229 1545 1439 1308 597 115 97
PasI CC'CwG_GG
Cuts at: 1348 2323 2379 1348
Size: 975 56 6299
Fragments arranged by size:
6299 975 56
PciI A'CATG_T
Cuts at: 3972 6229 3972
Size: 2257 5073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2329 2379 4131 4275 4551 1108
Size: 1221 50 1752 144 276 3887
Fragments arranged by size:
3887 1752 1221 276 144 50
PfoI T'CCnGG_A
Cuts at: 3156 3156
Size: 7330
PmeI GTTT'AAAC
Cuts at: 4634 4634
Size: 7330
PmlI CAC'GTG
Cuts at: 2581 4102 4275 2581
Size: 1521 173 5636
Fragments arranged by size:
5636 1521 173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5399 5431 6912 6944 3986
Size: 32 1381 32 1481 32 4372
Fragments arranged by size:
4372 1481 1381 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2747 3070 3526 3655 2747
Size: 323 456 129 6422
Fragments arranged by size:
6422 456 323 129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7330
PsiI TTA'TAA
Cuts at: 4900 4900
Size: 7330
PspOMI G'GGCC_C
Cuts at: 2585 2780 2852 4414 4505 2585
Size: 195 72 1562 91 5410
Fragments arranged by size:
5410 1562 195 91 72
PstI C_TGCA'G
Cuts at: 830 2105 2886 3056 3147 3387 3745 4177 4448
Size: 1275 781 170 91 240 358 432 271
Cuts at: 4448 4682 830
Size: 234 3478
Fragments arranged by size:
3478 1275 781 432 358 271 240 234
170 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7330
PvuII CAG'CTG
Cuts at: 3295 3295
Size: 7330
SacI G_AGCT'C
Cuts at: 3129 3357 3561 3129
Size: 228 204 6898
Fragments arranged by size:
6898 228 204
SalI G'TCGA_C
Cuts at: 1936 4672 1936
Size: 2736 4594
SapI GCTCTTCn'nnn_
Cuts at: 3618 3618
Size: 7330
SbfI CC_TGCA'GG
Cuts at: 3147 4682 3147
Size: 1535 5795
ScaI AGT'ACT
Cuts at: 5578 7290 5578
Size: 1712 5618
SexAI A'CCwGG_T
Cuts at: 3657 3657
Size: 7330
SfcI C'TryA_G
Cuts at: 826 1045 2101 2205 2882 3052 3143 3383 3741
Size: 219 1056 104 677 170 91 240 358
Cuts at: 3741 4173 4444 4678 5813 6494 6685 826
Size: 432 271 234 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 681 677 432 358 271
240 234 219 191 170 104 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 3108 3108
Size: 7330
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7330
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7330
SmaI CCC'GGG
Cuts at: 1872 2931 4657 1872
Size: 1059 1726 4545
Fragments arranged by size:
4545 1726 1059
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3284 5434 6335 6597 820
Size: 197 926 1341 2150 901 262 1553
Fragments arranged by size:
2150 1553 1341 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5121 493
Size: 4628 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7330
SphI G_CATG'C
Cuts at: 1132 4688 1132
Size: 3556 3774
SspI AAT'ATT
Cuts at: 5 52 5254 5
Size: 47 5202 2081
Fragments arranged by size:
5202 2081 47
StuI AGG'CCT
Cuts at: 1517 3374 4529 1517
Size: 1857 1155 4318
Fragments arranged by size:
4318 1857 1155
StyI C'CwwG_G
Cuts at: 513 1065 2286 2441 3327 3369 3432 3477 3855
Size: 552 1221 155 886 42 63 45 378
Cuts at: 3855 4155 4769 513
Size: 300 614 3074
Fragments arranged by size:
3074 1221 886 614 552 378 300 155
63 45 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2434 5357 5374 5527 5712 6157 325
Size: 1255 854 2923 17 153 185 445 1498
Fragments arranged by size:
2923 1498 1255 854 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4162 5576 7288
Size: 274 80 33 51 1443 2185 1414 1712
Cuts at: 7288 96
Size: 138
Fragments arranged by size:
2185 1712 1443 1414 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2275 2307 4065 4097 7290 7322 2275
Size: 32 1758 32 3193 32 2283
Fragments arranged by size:
3193 2283 1758 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7330
XbaI T'CTAG_A
Cuts at: 4666 4666
Size: 7330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3223 3310 3223
Size: 87 7243
XhoI C'TCGA_G
Cuts at: 1943 3284 1943
Size: 1341 5989
XmaI C'CCGG_G
Cuts at: 1870 2929 4655 1870
Size: 1059 1726 4545
Fragments arranged by size:
4545 1726 1059
XmnI GAAnn'nnTTC
Cuts at: 5459 5459
Size: 7330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3154 5138 276
Size: 53 83 186 936 1620 1984 2468
Fragments arranged by size:
2468 1984 1620 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI