Restriction map for
pFN21AE4235
(Circular) (Six-base) MAPSORT of: pFN21AE4235.seq Check: 5770 from: 1 to: 7279
pFN21AE4235 7279 bp
With 182 enzymes: SgfI *
October 29, 2018 21:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4527 5089 278
Size: 53 83 186 936 2991 562 2468
Fragments arranged by size:
2991 2468 936 562 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4622 1937
Size: 2685 4594
Acc65I G'GTAC_C
Cuts at: 4600 4600
Size: 7279
AclI AA'CG_TT
Cuts at: 1563 3579 5406 5779 1563
Size: 2016 1827 373 3063
Fragments arranged by size:
3063 2016 1827 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2332 5342 6726 6994 1632
Size: 700 3010 1384 268 1917
Fragments arranged by size:
3010 1917 1384 700 268
AflII C'TTAA_G
Cuts at: 820 1017 2927 4429 820
Size: 197 1910 1502 3670
Fragments arranged by size:
3670 1910 1502 197
AflIII A'CryG_T
Cuts at: 1203 2499 3597 6178 7155 1203
Size: 1296 1098 2581 977 1327
Fragments arranged by size:
2581 1327 1296 1098 977
AgeI A'CCGG_T
Cuts at: 6110 6110
Size: 7279
AhdI GACnn_n'nnGTC
Cuts at: 6008 6008
Size: 7279
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7279
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 7247
AlwNI CAG_nnn'CTG
Cuts at: 2962 3574 5022 6594 2962
Size: 612 1448 1572 3647
Fragments arranged by size:
3647 1572 1448 612
ApaI G_GGCC'C
Cuts at: 2786 2786
Size: 7279
ApaLI G'TGCA_C
Cuts at: 5336 6492 5336
Size: 1156 6123
ApoI r'AATT_y
Cuts at: 1493 2913 4588 4814 4966 7075 1493
Size: 1420 1675 226 152 2109 1697
Fragments arranged by size:
2109 1697 1675 1420 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5833 160
Size: 872 4801 1606
Fragments arranged by size:
4801 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2213 2269 4604 1870
Size: 73 114 156 56 2335 4545
Fragments arranged by size:
4545 2335 156 114 73 56
BamHI G'GATC_C
Cuts at: 3533 4609 4976 3533
Size: 1076 367 5836
Fragments arranged by size:
5836 1076 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 3896 4600 6055 618
Size: 325 217 627 486 1623 704 1455 1842
Fragments arranged by size:
1842 1623 1455 704 627 486 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2361 2448 2786 3892 3974 4166 4552
Size: 673 297 87 338 1106 82 192 386
Cuts at: 4552 4598 1391
Size: 46 4072
Fragments arranged by size:
4072 1106 673 386 338 297 192 87
82 46
BbeI G_GCGC'C
Cuts at: 2277 2277
Size: 7279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3043 3549 3600 928
Size: 971 1144 506 51 4607
Fragments arranged by size:
4607 1144 971 506 51
BbvCI CC'TCA_GC
Cuts at: 2741 3567 2741
Size: 826 6453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5470 5504 1903
Size: 34 50 34 3449 34 3678
Fragments arranged by size:
3678 3449 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3352 3707 5171 6381 3352
Size: 355 1464 1210 4250
Fragments arranged by size:
4250 1464 1210 355
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7279
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2271 5890
Size: 107 122 71 631 605 337 262 3619
Cuts at: 5890 136
Size: 1525
Fragments arranged by size:
3619 1525 631 605 337 262 122 107
71
BglII A'GATC_T
Cuts at: 2382 2895 7274 2382
Size: 513 4379 2387
Fragments arranged by size:
4379 2387 513
BlpI GC'TnA_GC
Cuts at: 2342 4160 4696 2342
Size: 1818 536 4925
Fragments arranged by size:
4925 1818 536
Bme1580I G_kGCm'C
Cuts at: 1790 2786 5340 6496 1790
Size: 996 2554 1156 2573
Fragments arranged by size:
2573 2554 1156 996
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2316 2377 3861
Size: 416 53 83 399 24 899 61 1484
Cuts at: 3861 4041 4473 5010 5968 442
Size: 180 432 537 958 1753
Fragments arranged by size:
1753 1484 958 899 537 432 416 399
180 83 61 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2561 2939 3025 3332 4148 4303 5939
Size: 1172 150 378 86 307 816 155 1636
Cuts at: 5939 1239
Size: 2579
Fragments arranged by size:
2579 1636 1172 816 378 307 155 150
86
Bpu10I CC'TnA_GC
Cuts at: 2640 2741 3567 2640
Size: 101 826 6352
Fragments arranged by size:
6352 826 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 3487 4523 5404 6269 6567 2432
Size: 1055 1036 881 865 298 3144
Fragments arranged by size:
3144 1055 1036 881 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 4218 5942 882
Size: 617 957 1762 1724 2219
Fragments arranged by size:
2219 1762 1724 957 617
BsaAI yAC'GTr
Cuts at: 493 1204 2502 2698 5070 493
Size: 711 1298 196 2372 2702
Fragments arranged by size:
2702 2372 1298 711 196
BsaBI GATnn'nnATC
Cuts at: 7273 7273
Size: 7279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 4524 5086 5468
Size: 53 83 186 936 741 2250 562 382
Cuts at: 5468 6103 275
Size: 635 1451
Fragments arranged by size:
2250 1451 936 741 635 562 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5712 6110 6384 6531 6942 5712
Size: 398 274 147 411 6049
Fragments arranged by size:
6049 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 2693 2723 3198
Size: 30 165 30 915 30 345 30 475
Cuts at: 3198 3228 4336 4366 4499 4529 1178
Size: 30 1108 30 133 30 3928
Fragments arranged by size:
3928 1108 915 475 345 165 133 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2456 2501 2743 2995 4046 4560 1186
Size: 1156 114 45 242 252 1051 514 3905
Fragments arranged by size:
3905 1156 1051 514 252 242 114 45
BseYI C'CCAG_C
Cuts at: 2173 2685 3097 3701 3842 3929 6482 6978 2173
Size: 512 412 604 141 87 2553 496 2474
Fragments arranged by size:
2553 2474 604 512 496 412 141 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 1163
Size: 573 564 6142
Fragments arranged by size:
6142 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4729 5490 6518 1540
Size: 275 186 2728 761 1028 2301
Fragments arranged by size:
2728 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2093 2361 2448 3892 4018 4349 5340 5425 6496
Size: 268 87 1444 126 331 991 85 1071
Cuts at: 6496 2093
Size: 2876
Fragments arranged by size:
2876 1444 1071 991 331 268 126 87
85
BsmI GAATG_Cn'
Cuts at: 4790 4883 4790
Size: 93 7186
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2093 2361 2448 2786 3892 3974
Size: 399 274 29 268 87 338 1106 82
Cuts at: 3974 4018 4166 4349 4552 4598 5340 5425 6496
Size: 44 148 183 203 46 742 85 1071
Cuts at: 6496 1391
Size: 2174
Fragments arranged by size:
2174 1106 1071 742 399 338 274 268
203 183 148 87 85 82 46 44
29
BspEI T'CCGG_A
Cuts at: 6942 6942
Size: 7279
BspHI T'CATG_A
Cuts at: 5168 6898 5168
Size: 1730 5549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2745 4634 844
Size: 1901 1889 3489
Fragments arranged by size:
3489 1901 1889
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 2476 5166 1394
Size: 429 214 439 2690 3507
Fragments arranged by size:
3507 2690 439 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 2613 3198 5774 5948 65
Size: 1183 1365 585 2576 174 1396
Fragments arranged by size:
2576 1396 1365 1183 585 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 5923 6110 1700
Size: 110 198 3915 187 2869
Fragments arranged by size:
3915 2869 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 3600 96
Size: 3504 3775
BssHII G'CGCG_C
Cuts at: 1925 2337 1925
Size: 412 6867
BssSI C'ACGA_G
Cuts at: 2129 4563 5339 6351 2129
Size: 2434 776 1012 3057
Fragments arranged by size:
3057 2434 1012 776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2088 794
Size: 1294 5985
BstBI TT'CG_AA
Cuts at: 6083 6083
Size: 7279
BstEII G'GTnAC_C
Cuts at: 1184 4040 1184
Size: 2856 4423
BstXI CCAn_nnnn'nTGG
Cuts at: 2948 4144 2948
Size: 1196 6083
BstYI r'GATC_y
Cuts at: 1972 2382 2571 2895 3533 4609 4976 5361 5378
Size: 410 189 324 638 1076 367 385 17
Cuts at: 5378 6830 6916 6928 7274 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1076 638 410 385 367 346
324 189 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3801 3801
Size: 7279
BtgI C'CryG_G
Cuts at: 513 1065 2064 2727 3021 4552 513
Size: 552 999 663 294 1531 3240
Fragments arranged by size:
3240 1531 999 663 552 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2238 2698 487
Size: 683 56 1012 460 5068
Fragments arranged by size:
5068 1012 683 460 56
BtsI GCAGTG_nn'
Cuts at: 4795 5589 5609 4795
Size: 794 20 6465
Fragments arranged by size:
6465 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7244
DraI TTT'AAA
Cuts at: 4583 4583
Size: 7279
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6286 809
Size: 507 4970 1802
Fragments arranged by size:
4970 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2665 2808 3612
Size: 54 1425 50 275 18 835 143 804
Cuts at: 3612 4726 5615 7244 8
Size: 1114 889 1629 43
Fragments arranged by size:
1629 1425 1114 889 835 804 275 143
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4726 1537
Size: 275 2914 4090
Fragments arranged by size:
4090 2914 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2510 3017 3228 3269 5209 1353
Size: 1157 507 211 41 1940 3423
Fragments arranged by size:
3423 1940 1157 507 211 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 2870 5866 6240 6386 387
Size: 1064 138 504 777 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 777 504 374 146 138
EcoICRI GAG'CTC
Cuts at: 2359 2446 3890 2359
Size: 87 1444 5748
Fragments arranged by size:
5748 1444 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2332 2411 2561 2939 3025 3332 4148
Size: 393 700 79 150 378 86 307 816
Cuts at: 4148 4303 5342 5939 6726 6994 1239
Size: 155 1039 597 787 268 1524
Fragments arranged by size:
1524 1039 816 787 700 597 393 378
307 268 155 150 86 79
EcoO109I rG'GnC_Cy
Cuts at: 2533 2782 4475 2533
Size: 249 1693 5337
Fragments arranged by size:
5337 1693 249
EcoRI G'AATT_C
Cuts at: 2913 4588 4966 2913
Size: 1675 378 5226
Fragments arranged by size:
5226 1675 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4422 4422
Size: 7279
FspI TGC'GCA
Cuts at: 2078 2509 5785 2078
Size: 431 3276 3572
Fragments arranged by size:
3572 3276 431
HaeII r_GCGC'y
Cuts at: 1678 2277 6426 1678
Size: 599 4149 2531
Fragments arranged by size:
4149 2531 599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4287 4319 4336 4368 5920
Size: 32 135 32 2715 32 17 32 1552
Cuts at: 5920 5952 5994 6026 1373
Size: 32 42 32 2626
Fragments arranged by size:
2715 2626 1552 135 42 32 32 32
32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 4027 4623 4869 669
Size: 988 281 2089 596 246 3079
Fragments arranged by size:
3079 2089 988 596 281 246
HindIII A'AGCT_T
Cuts at: 748 2930 748
Size: 2182 5097
HpaI GTT'AAC
Cuts at: 4869 4869
Size: 7279
KasI G'GCGC_C
Cuts at: 2273 2273
Size: 7279
KpnI G_GTAC'C
Cuts at: 4604 4604
Size: 7279
MfeI C'AATT_G
Cuts at: 4878 4878
Size: 7279
MluI A'CGCG_T
Cuts at: 7155 7155
Size: 7279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2874 3570 6393 6577 1305
Size: 1569 696 2823 184 2007
Fragments arranged by size:
2823 2007 1569 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2810 3614 10
Size: 54 1425 343 978 804 3675
Fragments arranged by size:
3675 1425 978 804 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3125 3350 3380 3746 5237 5596 5755
Size: 648 1959 225 30 366 1491 359 159
Cuts at: 5755 518
Size: 2042
Fragments arranged by size:
2042 1959 1491 648 366 359 225 159
30
MspA1I CmG'CkG
Cuts at: 1606 2689 4300 4695 5372 6520 6765 1606
Size: 1083 1611 395 677 1148 245 2120
Fragments arranged by size:
2120 1611 1148 1083 677 395 245
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 7279
NarI GG'CG_CC
Cuts at: 2274 2274
Size: 7279
NcoI C'CATG_G
Cuts at: 513 1065 2064 2727 3021 4552 513
Size: 552 999 663 294 1531 3240
Fragments arranged by size:
3240 1531 999 663 552 294
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7279
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 7279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7279
NotI GC'GGCC_GC
Cuts at: 4726 4726
Size: 7279
NspI r_CATG'y
Cuts at: 1132 1229 3601 3751 4637 6182 1132
Size: 97 2372 150 886 1545 2229
Fragments arranged by size:
2372 2229 1545 886 150 97
PasI CC'CwG_GG
Cuts at: 1348 4340 1348
Size: 2992 4287
PciI A'CATG_T
Cuts at: 3597 6178 3597
Size: 2581 4698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2766 2962 1108
Size: 1658 196 5425
Fragments arranged by size:
5425 1658 196
PmeI GTTT'AAAC
Cuts at: 4583 4583
Size: 7279
PmlI CAC'GTG
Cuts at: 2502 2698 2502
Size: 196 7083
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 5348 5380 6861 6893 4499
Size: 32 817 32 1481 32 4885
Fragments arranged by size:
4885 1481 817 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 2533
Size: 7279
PshAI GACnn'nnGTC
Cuts at: 1613 4047 1613
Size: 2434 4845
PsiI TTA'TAA
Cuts at: 4849 4849
Size: 7279
PspOMI G'GGCC_C
Cuts at: 2782 2782
Size: 7279
PspXI vC'TCGA_Gb
Cuts at: 2213 2213
Size: 7279
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4430 4462 4430
Size: 32 7247
PstI C_TGCA'G
Cuts at: 830 4631 830
Size: 3801 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7279
PvuII CAG'CTG
Cuts at: 2689 4300 2689
Size: 1611 5668
SacI G_AGCT'C
Cuts at: 2361 2448 3892 2361
Size: 87 1444 5748
Fragments arranged by size:
5748 1444 87
SalI G'TCGA_C
Cuts at: 1936 4621 1936
Size: 2685 4594
SapI GCTCTTCn'nnn_
Cuts at: 3228 3228
Size: 7279
SbfI CC_TGCA'GG
Cuts at: 4631 4631
Size: 7279
ScaI AGT'ACT
Cuts at: 2252 5527 7239 2252
Size: 3275 1712 2292
Fragments arranged by size:
3275 2292 1712
SfcI C'TryA_G
Cuts at: 826 1045 2230 2645 4306 4627 5762 6443 6634
Size: 219 1185 415 1661 321 1135 681 191
Cuts at: 6634 826
Size: 1471
Fragments arranged by size:
1661 1471 1185 1135 681 415 321 219
191
SfoI GGC'GCC
Cuts at: 2275 2275
Size: 7279
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7279
SmaI CCC'GGG
Cuts at: 1872 2271 4606 1872
Size: 399 2335 4545
Fragments arranged by size:
4545 2335 399
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 2447 2927 3502 4429 4502
Size: 197 926 270 234 480 575 927 73
Cuts at: 4502 5383 6284 6546 820
Size: 881 901 262 1553
Fragments arranged by size:
1553 927 926 901 881 575 480 270
262 234 197 73
SnaBI TAC'GTA
Cuts at: 493 5070 493
Size: 4577 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7279
SphI G_CATG'C
Cuts at: 1132 4637 1132
Size: 3505 3774
SspI AAT'ATT
Cuts at: 5 52 5203 5
Size: 47 5151 2081
Fragments arranged by size:
5151 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7279
StyI C'CwwG_G
Cuts at: 513 1065 2064 2727 3021 3522 3645 4552 4718
Size: 552 999 663 294 501 123 907 166
Cuts at: 4718 513
Size: 3074
Fragments arranged by size:
3074 999 907 663 552 501 294 166
123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3884 5306 5323 5476 5661 6106 325
Size: 1255 2304 1422 17 153 185 445 1498
Fragments arranged by size:
2304 1498 1422 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 3436 3600
Size: 274 80 33 51 1443 273 1186 164
Cuts at: 3600 4266 5525 7237 96
Size: 666 1259 1712 138
Fragments arranged by size:
1712 1443 1259 1186 666 274 273 164
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 2693 2725 3198 3230 7239 7271 2318
Size: 32 343 32 473 32 4009 32 2326
Fragments arranged by size:
4009 2326 473 343 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3487 1316
Size: 2171 5108
XbaI T'CTAG_A
Cuts at: 4615 4615
Size: 7279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2557 2803 3105 3166 3876 2557
Size: 246 302 61 710 5960
Fragments arranged by size:
5960 710 302 246 61
XhoI C'TCGA_G
Cuts at: 1943 2213 1943
Size: 270 7009
XmaI C'CCGG_G
Cuts at: 1870 2269 4604 1870
Size: 399 2335 4545
Fragments arranged by size:
4545 2335 399
XmnI GAAnn'nnTTC
Cuts at: 2774 5408 2774
Size: 2634 4645
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4525 5087 276
Size: 53 83 186 936 2991 562 2468
Fragments arranged by size:
2991 2468 936 562 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI
NsiI PacI PfoI RsrII SacII SanDI SexAI SfiI
SrfI SwaI