Restriction map for
pFN21AE4232
(Circular) (Six-base) MAPSORT of: pFN21AE4232.seq Check: 6396 from: 1 to: 7180
pFN21AE4232 7180 bp
With 182 enzymes: SgfI *
March 28, 2012 13:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4990 278
Size: 53 83 186 936 3454 2468
Fragments arranged by size:
3454 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4523 1937
Size: 2586 4594
Acc65I G'GTAC_C
Cuts at: 2466 4501 2466
Size: 2035 5145
AclI AA'CG_TT
Cuts at: 1563 5307 5680 1563
Size: 3744 373 3063
Fragments arranged by size:
3744 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2188 2302 2426 3532 3735 3935 4048 4076
Size: 556 114 124 1106 203 200 113 28
Cuts at: 4076 5243 6627 6895 1632
Size: 1167 1384 268 1917
Fragments arranged by size:
1917 1384 1167 1106 556 268 203 200
124 114 113 28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6983
AflIII A'CryG_T
Cuts at: 1203 4097 4188 6079 7056 1203
Size: 2894 91 1891 977 1327
Fragments arranged by size:
2894 1891 1327 977 91
AgeI A'CCGG_T
Cuts at: 6011 6011
Size: 7180
AhdI GACnn_n'nnGTC
Cuts at: 2542 5909 2542
Size: 3367 3813
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4091 1184
Size: 2907 4273
AlwNI CAG_nnn'CTG
Cuts at: 4923 6495 4923
Size: 1572 5608
ApaLI G'TGCA_C
Cuts at: 4380 5237 6393 4380
Size: 857 1156 5167
Fragments arranged by size:
5167 1156 857
ApoI r'AATT_y
Cuts at: 1493 2018 2908 4346 4412 4489 4715 4867 6976
Size: 525 890 1438 66 77 226 152 2109
Cuts at: 6976 1493
Size: 1697
Fragments arranged by size:
2109 1697 1438 890 525 226 152 77
66
AseI AT'TA_AT
Cuts at: 160 1032 5734 160
Size: 872 4702 1606
Fragments arranged by size:
4702 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7180
AvaI C'yCGr_G
Cuts at: 1870 1943 2396 2823 2898 3061 4505 1870
Size: 73 453 427 75 163 1444 4545
Fragments arranged by size:
4545 1444 453 427 163 75 73
BamHI G'GATC_C
Cuts at: 2323 4293 4510 4877 2323
Size: 1970 217 367 4626
Fragments arranged by size:
4626 1970 367 217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2216 2466 2741 2773
Size: 325 217 627 363 66 250 275 32
Cuts at: 2773 2784 2804 3232 3397 4014 4501 5956 618
Size: 11 20 428 165 617 487 1455 1842
Fragments arranged by size:
1842 1455 627 617 487 428 363 325
275 250 217 165 66 32 20 11
BanII G_rGCy'C
Cuts at: 1391 2285 3131 3746 4499 1391
Size: 894 846 615 753 4072
Fragments arranged by size:
4072 894 846 753 615
BbeI G_GCGC'C
Cuts at: 2745 3401 2745
Size: 656 6524
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6209
BbvCI CC'TCA_GC
Cuts at: 3179 3179
Size: 7180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5371 5405 1903
Size: 34 3434 34 3678
Fragments arranged by size:
3678 3434 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3693 5072 6282 3693
Size: 1379 1210 4591
Fragments arranged by size:
4591 1379 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7180
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5791 136
Size: 107 122 71 631 605 4119 1525
Fragments arranged by size:
4119 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7175 7175
Size: 7180
BlpI GC'TnA_GC
Cuts at: 2109 2592 2870 4597 2109
Size: 483 278 1727 4692
Fragments arranged by size:
4692 1727 483 278
Bme1580I G_kGCm'C
Cuts at: 1790 2219 3206 3355 3860 4384 5241 6397 1790
Size: 429 987 149 505 524 857 1156 2573
Fragments arranged by size:
2573 1156 987 857 524 505 429 149
BmgBI CAC'GTC
Cuts at: 1318 3999 1318
Size: 2681 4499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4911 5869 442
Size: 416 53 83 399 24 3494 958 1753
Fragments arranged by size:
3494 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7180
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3556 3556
Size: 7180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 2810 3551 5840 1239
Size: 1199 372 741 2289 2579
Fragments arranged by size:
2579 2289 1199 741 372
Bpu10I CC'TnA_GC
Cuts at: 2435 3125 3179 3207 3219 3745 2435
Size: 690 54 28 12 526 5870
Fragments arranged by size:
5870 690 526 54 28 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 5305 6170 6468 3227
Size: 2078 865 298 3939
Fragments arranged by size:
3939 2078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3154 5843 882
Size: 617 1655 2689 2219
Fragments arranged by size:
2689 2219 1655 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 3670 4971 493
Size: 711 1503 963 1301 2702
Fragments arranged by size:
2702 1503 1301 963 711
BsaBI GATnn'nnATC
Cuts at: 2127 2328 7174 2127
Size: 201 4846 2133
Fragments arranged by size:
4846 2133 201
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2742 2889 3159 3398
Size: 53 83 186 936 1209 147 270 239
Cuts at: 3398 3420 3521 4987 5369 6004 275
Size: 22 101 1466 382 635 1451
Fragments arranged by size:
1466 1451 1209 936 635 382 270 239
186 147 101 83 53 22
BsaWI w'CCGG_w
Cuts at: 2610 4275 5613 6011 6285 6432 6843 2610
Size: 1665 1338 398 274 147 411 2947
Fragments arranged by size:
2947 1665 1338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6955
Fragments arranged by size:
6955 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2169 2435 2438 2807 2918 3142 3532 3739
Size: 983 266 3 369 111 224 390 207
Cuts at: 3739 4446 1186
Size: 707 3920
Fragments arranged by size:
3920 983 707 390 369 266 224 207
111 3
BseYI C'CCAG_C
Cuts at: 2730 3130 3167 3304 3663 6383 6879 2730
Size: 400 37 137 359 2720 496 3031
Fragments arranged by size:
3031 2720 496 400 359 137 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 2669 1163
Size: 573 792 141 5674
Fragments arranged by size:
5674 792 573 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2476 4630 5391 6419 1540
Size: 275 186 475 2154 761 1028 2301
Fragments arranged by size:
2301 2154 1028 761 475 275 186
BsiHKAI G_wGCw'C
Cuts at: 3746 4384 5241 5326 6397 3746
Size: 638 857 85 1071 4529
Fragments arranged by size:
4529 1071 857 638 85
BsmI GAATG_Cn'
Cuts at: 4288 4691 4784 4288
Size: 403 93 6684
Fragments arranged by size:
6684 403 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3261 3261
Size: 7180
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2285 3131 3206 3355 3746 3860
Size: 399 429 66 846 75 149 391 114
Cuts at: 3860 4384 4499 5241 5326 6397 1391
Size: 524 115 742 85 1071 2174
Fragments arranged by size:
2174 1071 846 742 524 429 399 391
149 115 114 85 75 66
BspEI T'CCGG_A
Cuts at: 6843 6843
Size: 7180
BspHI T'CATG_A
Cuts at: 3954 5069 6799 3954
Size: 1115 1730 4335
Fragments arranged by size:
4335 1730 1115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2457 3036 3478 4292 4535 844
Size: 1241 372 579 442 814 243 3489
Fragments arranged by size:
3489 1241 814 579 442 372 243
BsrBI CCG'CTC
Cuts at: 1394 1823 3875 5067 1394
Size: 429 2052 1192 3507
Fragments arranged by size:
3507 2052 1192 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3615 3828 5675 5849 65
Size: 1183 2367 213 1847 174 1396
Fragments arranged by size:
2367 1847 1396 1183 213 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2295 2718 5824 6011 1700
Size: 110 234 251 423 3106 187 2869
Fragments arranged by size:
3106 2869 423 251 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7180
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7180
BssSI C'ACGA_G
Cuts at: 5240 6252 5240
Size: 1012 6168
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2911 794
Size: 2117 5063
BstBI TT'CG_AA
Cuts at: 5984 5984
Size: 7180
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7180
BstXI CCAn_nnnn'nTGG
Cuts at: 2738 2738
Size: 7180
BstYI r'GATC_y
Cuts at: 1972 2323 4293 4510 4877 5262 5279 6731 6817
Size: 351 1970 217 367 385 17 1452 86
Cuts at: 6817 6829 7175 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1970 1452 385 367 351 346 217
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 2159 2240 2285 2470 2880
Size: 552 939 72 83 81 45 185 410
Cuts at: 2880 3924 513
Size: 1044 3769
Fragments arranged by size:
3769 1044 939 552 410 185 83 81
72 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2099 3643 487
Size: 683 56 873 1544 4024
Fragments arranged by size:
4024 1544 873 683 56
BtsI GCAGTG_nn'
Cuts at: 3141 3503 3840 4127 4696 5490 5510 3141
Size: 362 337 287 569 794 20 4811
Fragments arranged by size:
4811 794 569 362 337 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7180
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7145
DraI TTT'AAA
Cuts at: 4484 4484
Size: 7180
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3653 6187 809
Size: 507 2337 2534 1802
Fragments arranged by size:
2534 2337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2473 2859 3819
Size: 54 1425 50 275 18 643 386 960
Cuts at: 3819 3876 4254 4627 5516 7145 8
Size: 57 378 373 889 1629 43
Fragments arranged by size:
1629 1425 960 889 643 386 378 373
275 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2473 4627 1537
Size: 275 661 2154 4090
Fragments arranged by size:
4090 2154 661 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2983 3711 5110 1353
Size: 1630 728 1399 3423
Fragments arranged by size:
3423 1630 1399 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5767 6141 6287 387
Size: 1064 138 4178 374 146 1280
Fragments arranged by size:
4178 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3744 3744
Size: 7180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2302 2426 2438 2810 3532 3551
Size: 393 556 114 124 12 372 722 19
Cuts at: 3551 3735 3935 4048 4076 5243 5840 6627 6895
Size: 184 200 113 28 1167 597 787 268
Cuts at: 6895 1239
Size: 1524
Fragments arranged by size:
1524 1167 787 722 597 556 393 372
268 200 184 124 114 113 28 19
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2840 3547 2840
Size: 707 6473
EcoO109I rG'GnC_Cy
Cuts at: 2097 2174 2190 2391 2446 3404 3931 2097
Size: 77 16 201 55 958 527 5346
Fragments arranged by size:
5346 958 527 201 77 55 16
EcoRI G'AATT_C
Cuts at: 4489 4867 4489
Size: 378 6802
FspI TGC'GCA
Cuts at: 2453 5686 2453
Size: 3233 3947
HaeII r_GCGC'y
Cuts at: 1678 2745 3179 3401 4162 6327 1678
Size: 1067 434 222 761 2165 2531
Fragments arranged by size:
2531 2165 1067 761 434 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2528 2560 2761 2793 3551
Size: 32 135 32 956 32 201 32 758
Cuts at: 3551 3583 5821 5853 5895 5927 1373
Size: 32 2238 32 42 32 2626
Fragments arranged by size:
2626 2238 956 758 201 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3197 3424 4524 4770 669
Size: 988 281 1259 227 1100 246 3079
Fragments arranged by size:
3079 1259 1100 988 281 246 227
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7180
HpaI GTT'AAC
Cuts at: 4770 4770
Size: 7180
KasI G'GCGC_C
Cuts at: 2741 3397 2741
Size: 656 6524
KpnI G_GTAC'C
Cuts at: 2470 4505 2470
Size: 2035 5145
MfeI C'AATT_G
Cuts at: 4779 4779
Size: 7180
MluI A'CGCG_T
Cuts at: 7056 7056
Size: 7180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6294 6478 1305
Size: 4989 184 2007
Fragments arranged by size:
4989 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2861 3821 10
Size: 54 1425 343 1029 960 3369
Fragments arranged by size:
3369 1425 1029 960 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 3737 3833 3899 5138 5497 5656
Size: 648 879 1692 96 66 1239 359 159
Cuts at: 5656 518
Size: 2042
Fragments arranged by size:
2042 1692 1239 879 648 359 159 96
66
MspA1I CmG'CkG
Cuts at: 1606 3784 4054 4596 5273 6421 6666 1606
Size: 2178 270 542 677 1148 245 2120
Fragments arranged by size:
2178 2120 1148 677 542 270 245
NaeI GCC'GGC
Cuts at: 2720 2720
Size: 7180
NarI GG'CG_CC
Cuts at: 2742 3398 2742
Size: 656 6524
NcoI C'CATG_G
Cuts at: 513 1065 2004 2076 2285 2880 3924 513
Size: 552 939 72 209 595 1044 3769
Fragments arranged by size:
3769 1044 939 595 552 209 72
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7180
NgoMIV G'CCGG_C
Cuts at: 2718 2718
Size: 7180
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7180
NotI GC'GGCC_GC
Cuts at: 4627 4627
Size: 7180
NspI r_CATG'y
Cuts at: 1132 1229 2044 2980 4101 4192 4538 6083 1132
Size: 97 815 936 1121 91 346 1545 2229
Fragments arranged by size:
2229 1545 1121 936 815 346 97 91
PasI CC'CwG_GG
Cuts at: 1348 2552 2916 3355 1348
Size: 1204 364 439 5173
Fragments arranged by size:
5173 1204 439 364
PciI A'CATG_T
Cuts at: 4097 4188 6079 4097
Size: 91 1891 5198
Fragments arranged by size:
5198 1891 91
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7180
PfoI T'CCnGG_A
Cuts at: 2274 3528 2274
Size: 1254 5926
PmeI GTTT'AAAC
Cuts at: 4484 4484
Size: 7180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5249 5281 6762 6794 3557
Size: 32 1660 32 1481 32 3943
Fragments arranged by size:
3943 1660 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 2174 2190 2391 2446 3404 3931 2097
Size: 77 16 201 55 958 527 5346
Fragments arranged by size:
5346 958 527 201 77 55 16
PshAI GACnn'nnGTC
Cuts at: 1613 2205 2491 1613
Size: 592 286 6302
Fragments arranged by size:
6302 592 286
PsiI TTA'TAA
Cuts at: 4750 4750
Size: 7180
PstI C_TGCA'G
Cuts at: 830 2014 4267 4532 830
Size: 1184 2253 265 3478
Fragments arranged by size:
3478 2253 1184 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7180
PvuII CAG'CTG
Cuts at: 3784 4054 3784
Size: 270 6910
SacI G_AGCT'C
Cuts at: 3746 3746
Size: 7180
SalI G'TCGA_C
Cuts at: 1936 4522 1936
Size: 2586 4594
SapI GCTCTTCn'nnn_
Cuts at: 2983 2983
Size: 7180
SbfI CC_TGCA'GG
Cuts at: 4532 4532
Size: 7180
ScaI AGT'ACT
Cuts at: 5428 7140 5428
Size: 1712 5468
SfcI C'TryA_G
Cuts at: 826 1045 2010 2638 4263 4528 5663 6344 6535
Size: 219 965 628 1625 265 1135 681 191
Cuts at: 6535 826
Size: 1471
Fragments arranged by size:
1625 1471 1135 965 681 628 265 219
191
SfoI GGC'GCC
Cuts at: 2743 3399 2743
Size: 656 6524
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7180
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7180
SmaI CCC'GGG
Cuts at: 1872 2825 4507 1872
Size: 953 1682 4545
Fragments arranged by size:
4545 1682 953
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 5284 6185 6447 820
Size: 197 926 1299 2042 901 262 1553
Fragments arranged by size:
2042 1553 1299 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4971 493
Size: 4478 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7180
SphI G_CATG'C
Cuts at: 1132 4538 1132
Size: 3406 3774
SspI AAT'ATT
Cuts at: 5 52 5104 5
Size: 47 5052 2081
Fragments arranged by size:
5052 2081 47
StuI AGG'CCT
Cuts at: 1517 2636 3750 1517
Size: 1119 1114 4947
Fragments arranged by size:
4947 1119 1114
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2285 2367 2841 2880 3461
Size: 552 939 72 209 82 474 39 581
Cuts at: 3461 3651 3924 4619 513
Size: 190 273 695 3074
Fragments arranged by size:
3074 939 695 581 552 474 273 209
190 82 72 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 3885 4040 5207 5224 5377 5562
Size: 1255 1293 1012 155 1167 17 153 185
Cuts at: 5562 6007 325
Size: 445 1498
Fragments arranged by size:
1498 1293 1255 1167 1012 445 185 155
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4020 5426 7138
Size: 274 80 33 51 1443 2043 1406 1712
Cuts at: 7138 96
Size: 138
Fragments arranged by size:
2043 1712 1443 1406 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 4087 4119 7140 7172 2219
Size: 32 1836 32 3021 32 2227
Fragments arranged by size:
3021 2227 1836 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7180
XbaI T'CTAG_A
Cuts at: 4516 4516
Size: 7180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2689 2689
Size: 7180
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7180
XmaI C'CCGG_G
Cuts at: 1870 2823 4505 1870
Size: 953 1682 4545
Fragments arranged by size:
4545 1682 953
XmnI GAAnn'nnTTC
Cuts at: 4061 5309 4061
Size: 1248 5932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4988 276
Size: 53 83 186 936 3454 2468
Fragments arranged by size:
3454 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI