Restriction map for
pFN21AE4231
(Circular) (Six-base) MAPSORT of: pFN21AE4231.seq Check: 4700 from: 1 to: 7150
pFN21AE4231 7150 bp
With 182 enzymes: SgfI *
March 28, 2012 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4960 278
Size: 53 83 186 936 3424 2468
Fragments arranged by size:
3424 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4493 1937
Size: 2556 4594
Acc65I G'GTAC_C
Cuts at: 4471 4471
Size: 7150
AclI AA'CG_TT
Cuts at: 1563 5277 5650 1563
Size: 3714 373 3063
Fragments arranged by size:
3714 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2628 3878 3884 4110 4221 4413 5213 6597
Size: 996 1250 6 226 111 192 800 1384
Cuts at: 6597 6865 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1250 996 800 268 226 192
111 6
AflII C'TTAA_G
Cuts at: 820 1017 2257 820
Size: 197 1240 5713
Fragments arranged by size:
5713 1240 197
AflIII A'CryG_T
Cuts at: 1203 6049 7026 1203
Size: 4846 977 1327
Fragments arranged by size:
4846 1327 977
AgeI A'CCGG_T
Cuts at: 5981 5981
Size: 7150
AhdI GACnn_n'nnGTC
Cuts at: 3435 5879 3435
Size: 2444 4706
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7150
AlwNI CAG_nnn'CTG
Cuts at: 4893 6465 4893
Size: 1572 5578
ApaLI G'TGCA_C
Cuts at: 5207 6363 5207
Size: 1156 5994
ApoI r'AATT_y
Cuts at: 1493 2157 2230 2297 2461 3883 4459 4685 4837
Size: 664 73 67 164 1422 576 226 152
Cuts at: 4837 6946 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1422 664 576 226 164 152
73 67
AseI AT'TA_AT
Cuts at: 160 1032 2274 4026 5704 160
Size: 872 1242 1752 1678 1606
Fragments arranged by size:
1752 1678 1606 1242 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7150
AvaI C'yCGr_G
Cuts at: 1870 1943 2481 4475 1870
Size: 73 538 1994 4545
Fragments arranged by size:
4545 1994 538 73
BamHI G'GATC_C
Cuts at: 2551 3632 4480 4847 2551
Size: 1081 848 367 4854
Fragments arranged by size:
4854 1081 848 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4471 5926 618
Size: 325 217 627 2684 1455 1842
Fragments arranged by size:
2684 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2672 3185 3650 4469 1391
Size: 1281 513 465 819 4072
Fragments arranged by size:
4072 1281 819 513 465
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6179
BbvCI CC'TCA_GC
Cuts at: 3237 3237
Size: 7150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5341 5375 1903
Size: 34 3404 34 3678
Fragments arranged by size:
3678 3404 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5042 6252 5042
Size: 1210 5940
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7150
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5761 136
Size: 107 122 71 631 605 4089 1525
Fragments arranged by size:
4089 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2435 7145 2435
Size: 4710 2440
BlpI GC'TnA_GC
Cuts at: 4567 4567
Size: 7150
Bme1580I G_kGCm'C
Cuts at: 1790 2019 5211 6367 1790
Size: 229 3192 1156 2573
Fragments arranged by size:
3192 2573 1156 229
BmgBI CAC'GTC
Cuts at: 1318 3342 1318
Size: 2024 5126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4881 5839 442
Size: 416 53 83 399 24 3464 958 1753
Fragments arranged by size:
3464 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 3831 4152 2374
Size: 1457 321 5372
Fragments arranged by size:
5372 1457 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3207 3443 4161 5810 1239
Size: 1968 236 718 1649 2579
Fragments arranged by size:
2579 1968 1649 718 236
Bpu10I CC'TnA_GC
Cuts at: 3237 3237
Size: 7150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2083 3634 5275 6140 6438 2083
Size: 1551 1641 865 298 2795
Fragments arranged by size:
2795 1641 1551 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5813 882
Size: 617 4314 2219
Fragments arranged by size:
4314 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4941 493
Size: 711 3737 2702
Fragments arranged by size:
3737 2702 711
BsaBI GATnn'nnATC
Cuts at: 2202 3060 3164 3327 7144 2202
Size: 858 104 163 3817 2208
Fragments arranged by size:
3817 2208 858 163 104
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4325 4957 5339 5974
Size: 53 83 186 936 2792 632 382 635
Cuts at: 5974 275
Size: 1451
Fragments arranged by size:
2792 1451 936 635 632 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3336 5583 5981 6255 6402 6813 3336
Size: 2247 398 274 147 411 3673
Fragments arranged by size:
3673 2247 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4387 4417 1178
Size: 30 165 30 2984 30 3911
Fragments arranged by size:
3911 2984 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3440 4395 1186
Size: 2254 955 3941
Fragments arranged by size:
3941 2254 955
BseYI C'CCAG_C
Cuts at: 6353 6849 6353
Size: 496 6654
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3339 1163
Size: 573 1603 4974
Fragments arranged by size:
4974 1603 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 4600 5361 6389 1540
Size: 275 186 45 2554 761 1028 2301
Fragments arranged by size:
2554 2301 1028 761 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 3185 3812 5211 5296 6367 3185
Size: 627 1399 85 1071 3968
Fragments arranged by size:
3968 1399 1071 627 85
BsmI GAATG_Cn'
Cuts at: 2920 4661 4754 2920
Size: 1741 93 5316
Fragments arranged by size:
5316 1741 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3480 3480
Size: 7150
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2672 3185 3650 3812 4469 5211
Size: 399 229 653 513 465 162 657 742
Cuts at: 5211 5296 6367 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 653 513 465 399
229 162 85
BspEI T'CCGG_A
Cuts at: 3336 6813 3336
Size: 3477 3673
BspHI T'CATG_A
Cuts at: 3085 5039 6769 3085
Size: 1954 1730 3466
Fragments arranged by size:
3466 1954 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 4505 844
Size: 2082 1579 3489
Fragments arranged by size:
3489 2082 1579
BsrBI CCG'CTC
Cuts at: 1394 1823 5037 1394
Size: 429 3214 3507
Fragments arranged by size:
3507 3214 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5645 5819 65
Size: 1183 4397 174 1396
Fragments arranged by size:
4397 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 5794 5981 1700
Size: 110 235 3749 187 2869
Fragments arranged by size:
3749 2869 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 3205 96
Size: 3109 4041
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7150
BssSI C'ACGA_G
Cuts at: 2884 5210 6222 2884
Size: 2326 1012 3812
Fragments arranged by size:
3812 2326 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2216 794
Size: 1422 5728
BstBI TT'CG_AA
Cuts at: 5954 5954
Size: 7150
BstEII G'GTnAC_C
Cuts at: 1184 2867 1184
Size: 1683 5467
BstXI CCAn_nnnn'nTGG
Cuts at: 3643 3686 3721 3643
Size: 43 35 7072
Fragments arranged by size:
7072 43 35
BstYI r'GATC_y
Cuts at: 1972 2203 2435 2551 3576 3632 3706 4480 4847
Size: 231 232 116 1025 56 74 774 367
Cuts at: 4847 5232 5249 6701 6787 6799 7145 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1025 774 385 367 346 232
231 116 86 74 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 513
Size: 552 939 30 5629
Fragments arranged by size:
5629 939 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6411
Fragments arranged by size:
6411 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 2374 2970 3714 4666 5460 5480 2017
Size: 357 596 744 952 794 20 3687
Fragments arranged by size:
3687 952 794 744 596 357 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7150
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7115
DraI TTT'AAA
Cuts at: 2229 2706 4454 2229
Size: 477 1748 4925
Fragments arranged by size:
4925 1748 477
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3226 6157 809
Size: 507 1910 2931 1802
Fragments arranged by size:
2931 1910 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2578 2880
Size: 54 1425 50 275 18 213 535 302
Cuts at: 2880 4597 5486 7115 8
Size: 1717 889 1629 43
Fragments arranged by size:
1717 1629 1425 889 535 302 275 213
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 4597 1537
Size: 275 231 2554 4090
Fragments arranged by size:
4090 2554 275 231
EarI CTCTTCn'nnn_
Cuts at: 1353 2861 3768 5080 1353
Size: 1508 907 1312 3423
Fragments arranged by size:
3423 1508 1312 907
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5737 6111 6257 387
Size: 1064 138 4148 374 146 1280
Fragments arranged by size:
4148 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3183 3183
Size: 7150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2628 3207 3443 3878 3884 4110 4161
Size: 393 996 579 236 435 6 226 51
Cuts at: 4161 4221 4413 5213 5810 6597 6865 1239
Size: 60 192 800 597 787 268 1524
Fragments arranged by size:
1524 996 800 787 597 579 435 393
268 236 226 192 60 51 6
EcoO109I rG'GnC_Cy
Cuts at: 3233 3470 3233
Size: 237 6913
EcoRI G'AATT_C
Cuts at: 4459 4837 4459
Size: 378 6772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 4443 3058
Size: 1385 5765
FspI TGC'GCA
Cuts at: 5656 5656
Size: 7150
HaeII r_GCGC'y
Cuts at: 1678 3664 6297 1678
Size: 1986 2633 2531
Fragments arranged by size:
2633 2531 1986
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3354 3361 3386 3393 3421
Size: 32 135 32 1782 7 25 7 28
Cuts at: 3421 3453 4118 4150 5791 5823 5865 5897 1373
Size: 32 665 32 1641 32 42 32 2626
Fragments arranged by size:
2626 1782 1641 665 135 42 32 32
32 32 32 32 28 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3589 3733 4494 4740 669
Size: 988 281 1651 144 761 246 3079
Fragments arranged by size:
3079 1651 988 761 281 246 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7150
HpaI GTT'AAC
Cuts at: 3733 4740 3733
Size: 1007 6143
KpnI G_GTAC'C
Cuts at: 4475 4475
Size: 7150
MfeI C'AATT_G
Cuts at: 4749 4749
Size: 7150
MluI A'CGCG_T
Cuts at: 7026 7026
Size: 7150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2089 2701 3788 6264 6448 1305
Size: 784 612 1087 2476 184 2007
Fragments arranged by size:
2476 2007 1087 784 612 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 2882 10
Size: 54 1425 343 748 302 4278
Fragments arranged by size:
4278 1425 748 343 302 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3026 3684 3719 5108 5467 5626 518
Size: 648 1860 658 35 1389 359 159 2042
Fragments arranged by size:
2042 1860 1389 658 648 359 159 35
MspA1I CmG'CkG
Cuts at: 1606 2036 2052 4566 5243 6391 6636 1606
Size: 430 16 2514 677 1148 245 2120
Fragments arranged by size:
2514 2120 1148 677 430 245 16
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 7150
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5659
Fragments arranged by size:
5659 939 552
NdeI CA'TA_TG
Cuts at: 387 2605 387
Size: 2218 4932
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 7150
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7150
NotI GC'GGCC_GC
Cuts at: 4597 4597
Size: 7150
NspI r_CATG'y
Cuts at: 1132 1229 4508 6053 1132
Size: 97 3279 1545 2229
Fragments arranged by size:
3279 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7150
PciI A'CATG_T
Cuts at: 6049 6049
Size: 7150
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2878 1108
Size: 1770 5380
PfoI T'CCnGG_A
Cuts at: 4138 4138
Size: 7150
PmeI GTTT'AAAC
Cuts at: 4454 4454
Size: 7150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5219 5251 6732 6764 5219
Size: 32 1481 32 5605
Fragments arranged by size:
5605 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3233 3470 3233
Size: 237 6913
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7150
PsiI TTA'TAA
Cuts at: 4720 4720
Size: 7150
PstI C_TGCA'G
Cuts at: 830 4502 830
Size: 3672 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7150
SacI G_AGCT'C
Cuts at: 3185 3185
Size: 7150
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 7150
SalI G'TCGA_C
Cuts at: 1936 4492 1936
Size: 2556 4594
SapI GCTCTTCn'nnn_
Cuts at: 3768 3768
Size: 7150
SbfI CC_TGCA'GG
Cuts at: 4502 4502
Size: 7150
ScaI AGT'ACT
Cuts at: 4339 5398 7110 4339
Size: 1059 1712 4379
Fragments arranged by size:
4379 1712 1059
SexAI A'CCwGG_T
Cuts at: 2398 4363 2398
Size: 1965 5185
SfcI C'TryA_G
Cuts at: 826 1045 2466 2844 4498 5633 6314 6505 826
Size: 219 1421 378 1654 1135 681 191 1471
Fragments arranged by size:
1654 1471 1421 1135 681 378 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7150
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7150
SmaI CCC'GGG
Cuts at: 1872 2483 4477 1872
Size: 611 1994 4545
Fragments arranged by size:
4545 1994 611
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2098 2257 3649 5254 6155 6417
Size: 197 926 155 159 1392 1605 901 262
Cuts at: 6417 820
Size: 1553
Fragments arranged by size:
1605 1553 1392 926 901 262 197 159
155
SnaBI TAC'GTA
Cuts at: 493 4941 493
Size: 4448 2702
SpeI A'CTAG_T
Cuts at: 152 2960 152
Size: 2808 4342
SphI G_CATG'C
Cuts at: 1132 4508 1132
Size: 3376 3774
SspI AAT'ATT
Cuts at: 5 52 5074 5
Size: 47 5022 2081
Fragments arranged by size:
5022 2081 47
StuI AGG'CCT
Cuts at: 1517 3949 1517
Size: 2432 4718
StyI C'CwwG_G
Cuts at: 513 1065 2004 3656 4297 4589 513
Size: 552 939 1652 641 292 3074
Fragments arranged by size:
3074 1652 939 641 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 2825 3148 3228 3932 5177 5194
Size: 1255 656 589 323 80 704 1245 17
Cuts at: 5194 5347 5532 5977 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1245 704 656 589 445 323
185 153 80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3205 3593 4337
Size: 274 80 33 51 1443 1228 388 744
Cuts at: 4337 4440 5396 7108 96
Size: 103 956 1712 138
Fragments arranged by size:
1712 1443 1228 956 744 388 274 138
103 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2532 2564 4170 4202 7110 7142 2532
Size: 32 1606 32 2908 32 2540
Fragments arranged by size:
2908 2540 1606 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7150
XbaI T'CTAG_A
Cuts at: 4486 4486
Size: 7150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2679 2679
Size: 7150
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7150
XmaI C'CCGG_G
Cuts at: 1870 2481 4475 1870
Size: 611 1994 4545
Fragments arranged by size:
4545 1994 611
XmnI GAAnn'nnTTC
Cuts at: 5279 5279
Size: 7150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4958 276
Size: 53 83 186 936 3424 2468
Fragments arranged by size:
3424 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SanDI SfiI SfoI SrfI
SwaI