Restriction map for
pFN21AE4224
(Circular) (Six-base) MAPSORT of: pFN21AE4224.seq Check: 267 from: 1 to: 7063
pFN21AE4224 7063 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3770 3770
Size: 7063
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4873 278
Size: 53 83 186 936 3337 2468
Fragments arranged by size:
3337 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3843 4406 1937
Size: 1906 563 4594
Fragments arranged by size:
4594 1906 563
Acc65I G'GTAC_C
Cuts at: 4384 4384
Size: 7063
AclI AA'CG_TT
Cuts at: 1563 5190 5563 1563
Size: 3627 373 3063
Fragments arranged by size:
3627 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5126 6510 6778 1632
Size: 3494 1384 268 1917
Fragments arranged by size:
3494 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6866
AflIII A'CryG_T
Cuts at: 1203 5962 6939 1203
Size: 4759 977 1327
Fragments arranged by size:
4759 1327 977
AgeI A'CCGG_T
Cuts at: 5894 5894
Size: 7063
AhdI GACnn_n'nnGTC
Cuts at: 2157 5792 2157
Size: 3635 3428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7063
AlwNI CAG_nnn'CTG
Cuts at: 2020 2034 3586 4806 6378 2020
Size: 14 1552 1220 1572 2705
Fragments arranged by size:
2705 1572 1552 1220 14
ApaLI G'TGCA_C
Cuts at: 5120 6276 5120
Size: 1156 5907
ApoI r'AATT_y
Cuts at: 1493 2270 2278 2357 2452 3340 4372 4598 4750
Size: 777 8 79 95 888 1032 226 152
Cuts at: 4750 6859 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1032 888 777 226 152 95
79 8
AseI AT'TA_AT
Cuts at: 160 1032 5617 160
Size: 872 4585 1606
Fragments arranged by size:
4585 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2540 4124 4203 4388 1870
Size: 73 147 450 1584 79 185 4545
Fragments arranged by size:
4545 1584 450 185 147 79 73
BamHI G'GATC_C
Cuts at: 2067 4393 4760 2067
Size: 2326 367 4370
Fragments arranged by size:
4370 2326 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4384 5839 618
Size: 325 217 627 2597 1455 1842
Fragments arranged by size:
2597 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2751 3759 4382 1391
Size: 730 630 1008 623 4072
Fragments arranged by size:
4072 1008 730 630 623
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3368 3392 928
Size: 971 1469 24 4599
Fragments arranged by size:
4599 1469 971 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5254 5288 1903
Size: 34 3317 34 3678
Fragments arranged by size:
3678 3317 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4224 4955 6165 4224
Size: 731 1210 5122
Fragments arranged by size:
5122 1210 731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7063
BfrBI ATG'CAT
Cuts at: 2250 3210 2250
Size: 960 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3796 5674 136
Size: 107 122 71 631 605 2124 1878 1525
Fragments arranged by size:
2124 1878 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2927 7058 2927
Size: 4131 2932
BlpI GC'TnA_GC
Cuts at: 4480 4480
Size: 7063
Bme1580I G_kGCm'C
Cuts at: 1790 5124 6280 1790
Size: 3334 1156 2573
Fragments arranged by size:
3334 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7063
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4027 4794 5752
Size: 416 53 83 399 24 2610 767 958
Cuts at: 5752 442
Size: 1753
Fragments arranged by size:
2610 1753 958 767 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2315 2952 3356 3878 4270 5723 1239
Size: 803 273 637 404 522 392 1453 2579
Fragments arranged by size:
2579 1453 803 637 522 404 392 273
Bpu10I CC'TnA_GC
Cuts at: 3753 3753
Size: 7063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5188 6053 6351 5188
Size: 865 298 5900
Fragments arranged by size:
5900 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5726 882
Size: 617 4227 2219
Fragments arranged by size:
4227 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2267 4854 493
Size: 711 1063 2587 2702
Fragments arranged by size:
2702 2587 1063 711
BsaBI GATnn'nnATC
Cuts at: 7057 7057
Size: 7063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3731 4870 5252 5887
Size: 53 83 186 936 2198 1139 382 635
Cuts at: 5887 275
Size: 1451
Fragments arranged by size:
2198 1451 1139 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5496 5894 6168 6315 6726 5496
Size: 398 274 147 411 5833
Fragments arranged by size:
5833 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6838
Fragments arranged by size:
6838 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3409 4140 1186
Size: 2223 731 4109
Fragments arranged by size:
4109 2223 731
BseYI C'CCAG_C
Cuts at: 2172 6266 6762 2172
Size: 4094 496 2473
Fragments arranged by size:
4094 2473 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6490
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4049 4513 5274 6302 1540
Size: 275 186 2048 464 761 1028 2301
Fragments arranged by size:
2301 2048 1028 761 464 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2751 2925 5124 5209 6280 2121
Size: 630 174 2199 85 1071 2904
Fragments arranged by size:
2904 2199 1071 630 174 85
BsmI GAATG_Cn'
Cuts at: 4574 4667 4574
Size: 93 6970
BsmBI CGTCTCn'nnnn_
Cuts at: 3942 3942
Size: 7063
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2751 2925 3759 4382 5124 5209
Size: 399 331 630 174 834 623 742 85
Cuts at: 5209 6280 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 834 742 630 623 399 331
174 85
BspEI T'CCGG_A
Cuts at: 6726 6726
Size: 7063
BspHI T'CATG_A
Cuts at: 2751 3414 4952 6682 2751
Size: 663 1538 1730 3132
Fragments arranged by size:
3132 1730 1538 663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3770 4418 844
Size: 2926 648 3489
Fragments arranged by size:
3489 2926 648
BsrBI CCG'CTC
Cuts at: 1394 1823 4950 1394
Size: 429 3127 3507
Fragments arranged by size:
3507 3127 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3328 5558 5732 65
Size: 1183 2080 2230 174 1396
Fragments arranged by size:
2230 2080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2514 5707 5894 1700
Size: 110 704 3193 187 2869
Fragments arranged by size:
3193 2869 704 187 110
BsrGI T'GTAC_A
Cuts at: 96 3252 4276 96
Size: 3156 1024 2883
Fragments arranged by size:
3156 2883 1024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7063
BssSI C'ACGA_G
Cuts at: 3228 3474 5123 6135 3228
Size: 246 1649 1012 4156
Fragments arranged by size:
4156 1649 1012 246
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7063
BstBI TT'CG_AA
Cuts at: 5867 5867
Size: 7063
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7063
BstXI CCAn_nnnn'nTGG
Cuts at: 3916 3916
Size: 7063
BstYI r'GATC_y
Cuts at: 1972 2067 2927 4393 4760 5145 5162 6614 6700
Size: 95 860 1466 367 385 17 1452 86
Cuts at: 6700 6712 7058 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1466 1452 860 385 367 346 95
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2051 3834 513
Size: 552 939 47 1783 3742
Fragments arranged by size:
3742 1783 939 552 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 487
Size: 683 56 902 5422
Fragments arranged by size:
5422 902 683 56
BtsI GCAGTG_nn'
Cuts at: 3095 4579 5373 5393 3095
Size: 1484 794 20 4765
Fragments arranged by size:
4765 1484 794 20
ClaI AT'CG_AT
Cuts at: 1554 2716 1554
Size: 1162 5901
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7028
DraI TTT'AAA
Cuts at: 4367 4367
Size: 7063
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4048 6070 809
Size: 507 2732 2022 1802
Fragments arranged by size:
2732 2022 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3837 4510 5399
Size: 54 1425 50 275 18 2007 673 889
Cuts at: 5399 7028 8
Size: 1629 43
Fragments arranged by size:
2007 1629 1425 889 673 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4510 1537
Size: 275 2698 4090
Fragments arranged by size:
4090 2698 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2739 3069 3800 4993 1353
Size: 1386 330 731 1193 3423
Fragments arranged by size:
3423 1386 1193 731 330
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5650 6024 6170 387
Size: 1064 138 4061 374 146 1280
Fragments arranged by size:
4061 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2119 2749 2119
Size: 630 6433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2315 2952 3356 3878 4270 5126
Size: 393 410 273 637 404 522 392 856
Cuts at: 5126 5723 6510 6778 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 856 787 637 597 522 410 404
393 392 273 268
EcoRI G'AATT_C
Cuts at: 3340 4372 4750 3340
Size: 1032 378 5653
Fragments arranged by size:
5653 1032 378
EcoRV GAT'ATC
Cuts at: 2497 2497
Size: 7063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2390 2390
Size: 7063
FspI TGC'GCA
Cuts at: 5569 5569
Size: 7063
HaeII r_GCGC'y
Cuts at: 1678 6210 1678
Size: 4532 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2143 2175 2912
Size: 32 135 32 487 32 52 32 737
Cuts at: 2912 2944 4007 4039 5704 5736 5778 5810 1373
Size: 32 1063 32 1665 32 42 32 2626
Fragments arranged by size:
2626 1665 1063 737 487 135 52 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4407 4653 669
Size: 988 281 2469 246 3079
Fragments arranged by size:
3079 2469 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7063
HpaI GTT'AAC
Cuts at: 4653 4653
Size: 7063
KpnI G_GTAC'C
Cuts at: 4388 4388
Size: 7063
MfeI C'AATT_G
Cuts at: 4662 4662
Size: 7063
MluI A'CGCG_T
Cuts at: 6939 6939
Size: 7063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3075 3156 3513 4106 4122 4175 6177 6361
Size: 1770 81 357 593 16 53 2002 184
Cuts at: 6361 1305
Size: 2007
Fragments arranged by size:
2007 2002 1770 593 357 184 81 53
16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5241
Fragments arranged by size:
5241 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2526 5021 5380 5539 518
Size: 648 1360 2495 359 159 2042
Fragments arranged by size:
2495 2042 1360 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 3953 4106 4479 5156 6304 6549 1606
Size: 570 1777 153 373 677 1148 245 2120
Fragments arranged by size:
2120 1777 1148 677 570 373 245 153
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5572
Fragments arranged by size:
5572 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7063
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7063
NotI GC'GGCC_GC
Cuts at: 4510 4510
Size: 7063
NsiI A_TGCA'T
Cuts at: 2252 3212 2252
Size: 960 6103
NspI r_CATG'y
Cuts at: 1132 1229 2881 3321 4421 5966 1132
Size: 97 1652 440 1100 1545 2229
Fragments arranged by size:
2229 1652 1545 1100 440 97
PasI CC'CwG_GG
Cuts at: 1348 3959 1348
Size: 2611 4452
PciI A'CATG_T
Cuts at: 5962 5962
Size: 7063
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3496 3991 1108
Size: 2388 495 4180
Fragments arranged by size:
4180 2388 495
PfoI T'CCnGG_A
Cuts at: 2058 2826 3967 2058
Size: 768 1141 5154
Fragments arranged by size:
5154 1141 768
PmeI GTTT'AAAC
Cuts at: 4367 4367
Size: 7063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2523 2555 5132 5164 6645 6677 2523
Size: 32 2577 32 1481 32 2909
Fragments arranged by size:
2909 2577 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7063
PsiI TTA'TAA
Cuts at: 4633 4633
Size: 7063
PspXI vC'TCGA_Gb
Cuts at: 4203 4203
Size: 7063
PstI C_TGCA'G
Cuts at: 830 2016 2562 3349 4415 830
Size: 1186 546 787 1066 3478
Fragments arranged by size:
3478 1186 1066 787 546
PvuI CG_AT'CG
Cuts at: 2001 4049 2001
Size: 2048 5015
PvuII CAG'CTG
Cuts at: 3953 4106 3953
Size: 153 6910
SacI G_AGCT'C
Cuts at: 2121 2751 2121
Size: 630 6433
SalI G'TCGA_C
Cuts at: 1936 4405 1936
Size: 2469 4594
SapI GCTCTTCn'nnn_
Cuts at: 2739 2739
Size: 7063
SbfI CC_TGCA'GG
Cuts at: 4415 4415
Size: 7063
ScaI AGT'ACT
Cuts at: 5311 7023 5311
Size: 1712 5351
SfcI C'TryA_G
Cuts at: 826 1045 2012 2558 2687 3345 3948 4161 4411
Size: 219 967 546 129 658 603 213 250
Cuts at: 4411 5546 6227 6418 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 967 681 658 603 546 250
219 213 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7063
SmaI CCC'GGG
Cuts at: 1872 4126 4390 1872
Size: 2254 264 4545
Fragments arranged by size:
4545 2254 264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2540 4203 5167 6068 6330
Size: 197 926 147 450 1663 964 901 262
Cuts at: 6330 820
Size: 1553
Fragments arranged by size:
1663 1553 964 926 901 450 262 197
147
SnaBI TAC'GTA
Cuts at: 493 4854 493
Size: 4361 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7063
SphI G_CATG'C
Cuts at: 1132 4421 1132
Size: 3289 3774
SspI AAT'ATT
Cuts at: 5 52 3685 4987 5
Size: 47 3633 1302 2081
Fragments arranged by size:
3633 2081 1302 47
StuI AGG'CCT
Cuts at: 1517 2839 1517
Size: 1322 5741
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 2775 3244 3794 3891 4502
Size: 552 939 345 426 469 550 97 611
Cuts at: 4502 513
Size: 3074
Fragments arranged by size:
3074 939 611 552 550 469 426 345
97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3095 5090 5107 5260 5445 5890 325
Size: 1255 1515 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1515 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3252 4276 5309
Size: 274 80 33 51 1443 1275 1024 1033
Cuts at: 5309 7021 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1275 1033 1024 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7023 7055 7023
Size: 32 7031
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7063
XbaI T'CTAG_A
Cuts at: 4399 4399
Size: 7063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3832 3832
Size: 7063
XhoI C'TCGA_G
Cuts at: 1943 2090 2540 4203 1943
Size: 147 450 1663 4803
Fragments arranged by size:
4803 1663 450 147
XmaI C'CCGG_G
Cuts at: 1870 4124 4388 1870
Size: 2254 264 4545
Fragments arranged by size:
4545 2254 264
XmnI GAAnn'nnTTC
Cuts at: 5192 5192
Size: 7063
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4871 276
Size: 53 83 186 936 3337 2468
Fragments arranged by size:
3337 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI