Restriction map for
pFN21AE4220
(Circular) (Six-base) MAPSORT of: pFN21AE4220.seq Check: 1911 from: 1 to: 6982
pFN21AE4220 6982 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3385 3579 3385
Size: 194 6788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4792 278
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3798 4325 1937
Size: 1861 527 4594
Fragments arranged by size:
4594 1861 527
Acc65I G'GTAC_C
Cuts at: 2481 4303 2481
Size: 1822 5160
AclI AA'CG_TT
Cuts at: 1563 5109 5482 1563
Size: 3546 373 3063
Fragments arranged by size:
3546 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2984 3204 5045 6429 6697 1632
Size: 1352 220 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 1352 268 220
AflII C'TTAA_G
Cuts at: 820 1017 2321 3113 820
Size: 197 1304 792 4689
Fragments arranged by size:
4689 1304 792 197
AflIII A'CryG_T
Cuts at: 1203 2157 5881 6858 1203
Size: 954 3724 977 1327
Fragments arranged by size:
3724 1327 977 954
AgeI A'CCGG_T
Cuts at: 5813 5813
Size: 6982
AhdI GACnn_n'nnGTC
Cuts at: 5711 5711
Size: 6982
AleI CACnn'nnGTG
Cuts at: 1166 2789 2912 3413 3732 1166
Size: 1623 123 501 319 4416
Fragments arranged by size:
4416 1623 501 319 123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3431 4004 1184
Size: 2247 573 4162
Fragments arranged by size:
4162 2247 573
AlwNI CAG_nnn'CTG
Cuts at: 2134 3590 3783 4172 4725 6297 2134
Size: 1456 193 389 553 1572 2819
Fragments arranged by size:
2819 1572 1456 553 389 193
ApaLI G'TGCA_C
Cuts at: 3566 5039 6195 3566
Size: 1473 1156 4353
Fragments arranged by size:
4353 1473 1156
ApoI r'AATT_y
Cuts at: 1493 4291 4517 4669 6778 1493
Size: 2798 226 152 2109 1697
Fragments arranged by size:
2798 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5536 160
Size: 872 4504 1606
Fragments arranged by size:
4504 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
AvaI C'yCGr_G
Cuts at: 1870 1943 2175 3174 4307 1870
Size: 73 232 999 1133 4545
Fragments arranged by size:
4545 1133 999 232 73
BamHI G'GATC_C
Cuts at: 4312 4679 4312
Size: 367 6615
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2481 2507 2618 3467 3866
Size: 325 217 627 694 26 111 849 399
Cuts at: 3866 3920 4303 5758 618
Size: 54 383 1455 1842
Fragments arranged by size:
1842 1455 849 694 627 399 383 325
217 111 54 26
BanII G_rGCy'C
Cuts at: 1391 2235 2643 3503 4143 4301 1391
Size: 844 408 860 640 158 4072
Fragments arranged by size:
4072 860 844 640 408 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6011
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3902 3936 5173 5207 1903
Size: 34 1965 34 1237 34 3678
Fragments arranged by size:
3678 1965 1237 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4874 6084 4874
Size: 1210 5772
BclI T'GATC_A
Cuts at: 1548 2379 3334 3879 1548
Size: 831 955 545 4651
Fragments arranged by size:
4651 955 831 545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2196 2807 3621
Size: 107 122 71 631 605 524 611 814
Cuts at: 3621 5593 136
Size: 1972 1525
Fragments arranged by size:
1972 1525 814 631 611 605 524 122
107 71
BglII A'GATC_T
Cuts at: 2877 3339 6977 2877
Size: 462 3638 2882
Fragments arranged by size:
3638 2882 462
BlpI GC'TnA_GC
Cuts at: 3423 4399 3423
Size: 976 6006
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2176 2396 2621 2687 2778 3470 3478
Size: 315 71 220 225 66 91 692 8
Cuts at: 3478 3570 3871 3912 4062 4154 4181 4235 5043
Size: 92 301 41 150 92 27 54 808
Cuts at: 5043 6199 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 808 692 315 301 225 220
150 92 92 91 71 66 54 41
27 8
BmgBI CAC'GTC
Cuts at: 1318 3244 3251 3757 1318
Size: 1926 7 506 4543
Fragments arranged by size:
4543 1926 506 7
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 3793 4713
Size: 416 53 83 399 24 682 1694 920
Cuts at: 4713 5671 442
Size: 958 1753
Fragments arranged by size:
1753 1694 958 920 682 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6982
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 6982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2909 2996 3176 3857 4202 5642 1239
Size: 1244 426 87 180 681 345 1440 2579
Fragments arranged by size:
2579 1440 1244 681 426 345 180 87
Bpu10I CC'TnA_GC
Cuts at: 3082 4165 3082
Size: 1083 5899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2798 3503 5107 5972 6270 2798
Size: 705 1604 865 298 3510
Fragments arranged by size:
3510 1604 865 705 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5645 882
Size: 617 4146 2219
Fragments arranged by size:
4146 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4773 493
Size: 711 3569 2702
Fragments arranged by size:
3569 2702 711
BsaBI GATnn'nnATC
Cuts at: 6976 6976
Size: 6982
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3828 3944 4789 5171
Size: 53 83 186 936 2295 116 845 382
Cuts at: 5171 5806 275
Size: 635 1451
Fragments arranged by size:
2295 1451 936 845 635 382 186 116
83 53
BsaWI w'CCGG_w
Cuts at: 2448 3063 5415 5813 6087 6234 6645 2448
Size: 615 2352 398 274 147 411 2785
Fragments arranged by size:
2785 2352 615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2927 2957 3791
Size: 30 165 30 807 30 687 30 834
Cuts at: 3791 3821 1178
Size: 30 4339
Fragments arranged by size:
4339 834 807 687 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2906 2951 2993 3208 3470 1186
Size: 1720 45 42 215 262 4698
Fragments arranged by size:
4698 1720 262 215 45 42
BseYI C'CCAG_C
Cuts at: 2222 2395 2631 2962 3315 3583 3618 3802 3991
Size: 173 236 331 353 268 35 184 189
Cuts at: 3991 6185 6681 2222
Size: 2194 496 2523
Fragments arranged by size:
2523 2194 496 353 331 268 236 189
184 173 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2545 3143 3557 3581 4036 1163
Size: 573 809 598 414 24 455 4109
Fragments arranged by size:
4109 809 598 573 455 414 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4432 5193 6221 1540
Size: 275 186 2431 761 1028 2301
Fragments arranged by size:
2431 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2796 3063 3570 5043 5128 6199 2796
Size: 267 507 1473 85 1071 3579
Fragments arranged by size:
3579 1473 1071 507 267 85
BsmI GAATG_Cn'
Cuts at: 3020 4493 4586 3020
Size: 1473 93 5416
Fragments arranged by size:
5416 1473 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3044 3044
Size: 6982
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2176 2235 2396 2621 2643 2687
Size: 399 315 71 59 161 225 22 44
Cuts at: 2687 2778 2796 3063 3470 3478 3503 3570 3871
Size: 91 18 267 407 8 25 67 301
Cuts at: 3871 3912 4062 4143 4154 4181 4235 4301 5043
Size: 41 150 81 11 27 54 66 742
Cuts at: 5043 5128 6199 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 407 399 315 301 267
225 161 150 91 85 81 71 67
66 59 54 44 41 27 25 22
18 11 8
BspEI T'CCGG_A
Cuts at: 2448 6645 2448
Size: 4197 2785
BspHI T'CATG_A
Cuts at: 4871 6601 4871
Size: 1730 5252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3385 3579 4337 844
Size: 2541 194 758 3489
Fragments arranged by size:
3489 2541 758 194
BsrBI CCG'CTC
Cuts at: 1394 1823 2428 2479 3028 3637 4099 4869 1394
Size: 429 605 51 549 609 462 770 3507
Fragments arranged by size:
3507 770 609 605 549 462 429 51
BsrDI GCAATG_nn'
Cuts at: 65 1248 2593 3229 5477 5651 65
Size: 1183 1345 636 2248 174 1396
Fragments arranged by size:
2248 1396 1345 1183 636 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 3539 3708 3719 4046 5626 5813
Size: 110 503 1226 169 11 327 1580 187
Cuts at: 5813 1700
Size: 2869
Fragments arranged by size:
2869 1580 1226 503 327 187 169 110
11
BsrGI T'GTAC_A
Cuts at: 96 2121 96
Size: 2025 4957
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6982
BssSI C'ACGA_G
Cuts at: 5042 6054 5042
Size: 1012 5970
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2568 2592 2724 2953 794
Size: 1774 24 132 229 4823
Fragments arranged by size:
4823 1774 229 132 24
BstBI TT'CG_AA
Cuts at: 5786 5786
Size: 6982
BstEII G'GTnAC_C
Cuts at: 1184 2704 1184
Size: 1520 5462
BstXI CCAn_nnnn'nTGG
Cuts at: 3381 3976 3381
Size: 595 6387
BstYI r'GATC_y
Cuts at: 1972 2877 3051 3339 3984 4312 4679 5064 5081
Size: 905 174 288 645 328 367 385 17
Cuts at: 5081 6533 6619 6631 6977 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 905 645 385 367 346 328
288 174 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3294 3294
Size: 6982
BtgI C'CryG_G
Cuts at: 513 1065 2004 3523 4135 513
Size: 552 939 1519 612 3360
Fragments arranged by size:
3360 1519 939 612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6243
Fragments arranged by size:
6243 683 56
BtsI GCAGTG_nn'
Cuts at: 2993 4498 5292 5312 2993
Size: 1505 794 20 4663
Fragments arranged by size:
4663 1505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6947
DraI TTT'AAA
Cuts at: 4286 4286
Size: 6982
DraIII CAC_nnn'GTG
Cuts at: 2158 2568 2158
Size: 410 6572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2054 5989 809
Size: 507 738 3935 1802
Fragments arranged by size:
3935 1802 738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3203 4429 5318
Size: 54 1425 50 275 18 1373 1226 889
Cuts at: 5318 6947 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1373 1226 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4429 1537
Size: 275 2617 4090
Fragments arranged by size:
4090 2617 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2609 2694 4912 1353
Size: 1256 85 2218 3423
Fragments arranged by size:
3423 2218 1256 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3215 5569 5943 6089 387
Size: 1064 138 1626 2354 374 146 1280
Fragments arranged by size:
2354 1626 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2483 2909 2984 2996 3176 3204 3857
Size: 393 851 426 75 12 180 28 653
Cuts at: 3857 4202 5045 5642 6429 6697 1239
Size: 345 843 597 787 268 1524
Fragments arranged by size:
1524 851 843 787 653 597 426 393
345 268 180 75 28 12
EcoO109I rG'GnC_Cy
Cuts at: 2218 2674 2809 3298 3431 4169 2218
Size: 456 135 489 133 738 5031
Fragments arranged by size:
5031 738 489 456 135 133
EcoRI G'AATT_C
Cuts at: 4291 4669 4291
Size: 378 6604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 6982
FspI TGC'GCA
Cuts at: 5488 5488
Size: 6982
HaeII r_GCGC'y
Cuts at: 1678 3048 6129 1678
Size: 1370 3081 2531
Fragments arranged by size:
3081 2531 1370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 2496 2528 2927
Size: 32 135 32 638 32 254 32 399
Cuts at: 2927 2959 3485 3517 3791 3823 5623 5655 5697
Size: 32 526 32 274 32 1800 32 42
Cuts at: 5697 5729 1373
Size: 32 2626
Fragments arranged by size:
2626 1800 638 526 399 274 254 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2744 4326 4572 669
Size: 988 281 806 1582 246 3079
Fragments arranged by size:
3079 1582 988 806 281 246
HindIII A'AGCT_T
Cuts at: 748 3845 748
Size: 3097 3885
HpaI GTT'AAC
Cuts at: 4572 4572
Size: 6982
KpnI G_GTAC'C
Cuts at: 2485 4307 2485
Size: 1822 5160
MfeI C'AATT_G
Cuts at: 4581 4581
Size: 6982
MluI A'CGCG_T
Cuts at: 6858 6858
Size: 6982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2689 6096 6280 1305
Size: 1384 3407 184 2007
Fragments arranged by size:
3407 2007 1384 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5160
Fragments arranged by size:
5160 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 2912 3413 3732 3974 4940 5299
Size: 648 1623 123 501 319 242 966 359
Cuts at: 5299 5458 518
Size: 159 2042
Fragments arranged by size:
2042 1623 966 648 501 359 319 242
159 123
MspA1I CmG'CkG
Cuts at: 1606 2772 2950 3157 3209 3441 3448 3525 3618
Size: 1166 178 207 52 232 7 77 93
Cuts at: 3618 3906 3918 4398 5075 6223 6468 1606
Size: 288 12 480 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 480 288 245 232
207 178 93 77 52 12 7
NaeI GCC'GGC
Cuts at: 3541 4048 3541
Size: 507 6475
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5491
Fragments arranged by size:
5491 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6982
NgoMIV G'CCGG_C
Cuts at: 3539 4046 3539
Size: 507 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6982
NotI GC'GGCC_GC
Cuts at: 4429 4429
Size: 6982
NspI r_CATG'y
Cuts at: 1132 1229 2727 4340 5885 1132
Size: 97 1498 1613 1545 2229
Fragments arranged by size:
2229 1613 1545 1498 97
PasI CC'CwG_GG
Cuts at: 1348 2345 2613 3769 1348
Size: 997 268 1156 4561
Fragments arranged by size:
4561 1156 997 268
PciI A'CATG_T
Cuts at: 5881 5881
Size: 6982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 3441 1108
Size: 1626 707 4649
Fragments arranged by size:
4649 1626 707
PfoI T'CCnGG_A
Cuts at: 3054 4116 4218 3054
Size: 1062 102 5818
Fragments arranged by size:
5818 1062 102
PmeI GTTT'AAAC
Cuts at: 4286 4286
Size: 6982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5051 5083 6564 6596 5051
Size: 32 1481 32 5437
Fragments arranged by size:
5437 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 2218
Size: 6982
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6982
PsiI TTA'TAA
Cuts at: 4552 4552
Size: 6982
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3660 3692 3660
Size: 32 6950
PstI C_TGCA'G
Cuts at: 830 4334 830
Size: 3504 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6982
PvuII CAG'CTG
Cuts at: 2950 3157 3209 2950
Size: 207 52 6723
Fragments arranged by size:
6723 207 52
SacII CC_GC'GG
Cuts at: 3526 3526
Size: 6982
SalI G'TCGA_C
Cuts at: 1936 4324 1936
Size: 2388 4594
SanDI GG'GwC_CC
Cuts at: 2218 2218
Size: 6982
SapI GCTCTTCn'nnn_
Cuts at: 2609 2609
Size: 6982
SbfI CC_TGCA'GG
Cuts at: 4334 4334
Size: 6982
ScaI AGT'ACT
Cuts at: 3111 5230 6942 3111
Size: 2119 1712 3151
Fragments arranged by size:
3151 2119 1712
SfcI C'TryA_G
Cuts at: 826 1045 4330 5465 6146 6337 826
Size: 219 3285 1135 681 191 1471
Fragments arranged by size:
3285 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6982
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6982
SmaI CCC'GGG
Cuts at: 1872 4309 1872
Size: 2437 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 2813 3113 3482 5086 5987
Size: 197 926 378 492 300 369 1604 901
Cuts at: 5987 6249 820
Size: 262 1553
Fragments arranged by size:
1604 1553 926 901 492 378 369 300
262 197
SnaBI TAC'GTA
Cuts at: 493 4773 493
Size: 4280 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6982
SphI G_CATG'C
Cuts at: 1132 4340 1132
Size: 3208 3774
SspI AAT'ATT
Cuts at: 5 52 4906 5
Size: 47 4854 2081
Fragments arranged by size:
4854 2081 47
StuI AGG'CCT
Cuts at: 1517 2168 1517
Size: 651 6331
StyI C'CwwG_G
Cuts at: 513 1065 2004 2404 2553 2670 2931 4421 513
Size: 552 939 400 149 117 261 1490 3074
Fragments arranged by size:
3074 1490 939 552 400 261 149 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 2780 3329 5009 5026 5179 5364
Size: 1255 1056 144 549 1680 17 153 185
Cuts at: 5364 5809 325
Size: 445 1498
Fragments arranged by size:
1680 1498 1255 1056 549 445 185 153
144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 3109 4210
Size: 274 80 33 51 1443 144 988 1101
Cuts at: 4210 5228 6940 96
Size: 1018 1712 138
Fragments arranged by size:
1712 1443 1101 1018 988 274 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 2344 2376 6942 6974 2084
Size: 32 228 32 4566 32 2092
Fragments arranged by size:
4566 2092 228 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6982
XbaI T'CTAG_A
Cuts at: 4318 4318
Size: 6982
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6982
XmaI C'CCGG_G
Cuts at: 1870 4307 1870
Size: 2437 4545
XmnI GAAnn'nnTTC
Cuts at: 5111 5111
Size: 6982
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4790 276
Size: 53 83 186 936 3256 2468
Fragments arranged by size:
3256 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacI SexAI SfiI SfoI SrfI SwaI XcmI