Restriction map for
pFN21AE4219
(Circular) (Six-base) MAPSORT of: pFN21AE4219.seq Check: 419 from: 1 to: 6859
pFN21AE4219 6859 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4669 278
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 4202 1937
Size: 166 2099 4594
Fragments arranged by size:
4594 2099 166
Acc65I G'GTAC_C
Cuts at: 4180 4180
Size: 6859
AclI AA'CG_TT
Cuts at: 1563 4158 4986 5359 1563
Size: 2595 828 373 3063
Fragments arranged by size:
3063 2595 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3506 3769 4922 6306 6574 1632
Size: 1874 263 1153 1384 268 1917
Fragments arranged by size:
1917 1874 1384 1153 268 263
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6662
AflIII A'CryG_T
Cuts at: 1203 5758 6735 1203
Size: 4555 977 1327
Fragments arranged by size:
4555 1327 977
AgeI A'CCGG_T
Cuts at: 5690 5690
Size: 6859
AhdI GACnn_n'nnGTC
Cuts at: 2509 5588 2509
Size: 3079 3780
AleI CACnn'nnGTG
Cuts at: 1166 2456 1166
Size: 1290 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2228 1184
Size: 1044 5815
AlwNI CAG_nnn'CTG
Cuts at: 2032 2299 4602 6174 2032
Size: 267 2303 1572 2717
Fragments arranged by size:
2717 2303 1572 267
ApaI G_GGCC'C
Cuts at: 2114 2141 2114
Size: 27 6832
ApaLI G'TGCA_C
Cuts at: 4916 6072 4916
Size: 1156 5703
ApoI r'AATT_y
Cuts at: 1493 3467 4168 4394 4546 6655 1493
Size: 1974 701 226 152 2109 1697
Fragments arranged by size:
2109 1974 1697 701 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5413 160
Size: 872 4381 1606
Fragments arranged by size:
4381 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 2264 4184 1870
Size: 73 298 23 1920 4545
Fragments arranged by size:
4545 1920 298 73 23
BamHI G'GATC_C
Cuts at: 4189 4556 4189
Size: 367 6492
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2368 2374 2405 4180 5635
Size: 325 217 627 581 6 31 1775 1455
Cuts at: 5635 618
Size: 1842
Fragments arranged by size:
1842 1775 1455 627 581 325 217 31
6
BanII G_rGCy'C
Cuts at: 1391 2114 2141 2641 2955 3459 3882 4072 4178
Size: 723 27 500 314 504 423 190 106
Cuts at: 4178 1391
Size: 4072
Fragments arranged by size:
4072 723 504 500 423 314 190 106
27
BbeI G_GCGC'C
Cuts at: 2372 2378 2372
Size: 6 6853
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3852 928
Size: 971 1953 3935
Fragments arranged by size:
3935 1953 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5050 5084 1903
Size: 34 3113 34 3678
Fragments arranged by size:
3678 3113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3951 4751 5961 3951
Size: 800 1210 4849
Fragments arranged by size:
4849 1210 800
BclI T'GATC_A
Cuts at: 1548 3217 1548
Size: 1669 5190
BfrBI ATG'CAT
Cuts at: 3639 3639
Size: 6859
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2438 2645 2703
Size: 107 122 71 631 605 766 207 58
Cuts at: 2703 5470 136
Size: 2767 1525
Fragments arranged by size:
2767 1525 766 631 605 207 122 107
71 58
BglII A'GATC_T
Cuts at: 6854 6854
Size: 6859
BlpI GC'TnA_GC
Cuts at: 2033 2307 2690 3876 4071 4276 2033
Size: 274 383 1186 195 205 4616
Fragments arranged by size:
4616 1186 383 274 205 195
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2141 4920 6076 1790
Size: 324 27 2779 1156 2573
Fragments arranged by size:
2779 2573 1156 324 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6859
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2631 4590 5548
Size: 416 53 83 399 24 1214 1959 958
Cuts at: 5548 442
Size: 1753
Fragments arranged by size:
1959 1753 1214 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4091
Size: 6859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2969 5519 1239
Size: 1097 633 2550 2579
Fragments arranged by size:
2579 2550 1097 633
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 4984 5849 6147 2780
Size: 2204 865 298 3492
Fragments arranged by size:
3492 2204 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2859 5522 882
Size: 617 1360 2663 2219
Fragments arranged by size:
2663 2219 1360 617
BsaAI yAC'GTr
Cuts at: 493 1204 4650 493
Size: 711 3446 2702
Fragments arranged by size:
3446 2702 711
BsaBI GATnn'nnATC
Cuts at: 6853 6853
Size: 6859
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 2237 2369 2375
Size: 53 83 186 936 696 8 132 6
Cuts at: 2375 3993 4666 5048 5683 275
Size: 1618 673 382 635 1451
Fragments arranged by size:
1618 1451 936 696 673 635 382 186
132 83 53 8 6
BsaWI w'CCGG_w
Cuts at: 5292 5690 5964 6111 6522 5292
Size: 398 274 147 411 5629
Fragments arranged by size:
5629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3065 3095 3669 3699 1178
Size: 30 165 30 1662 30 574 30 4338
Fragments arranged by size:
4338 1662 574 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 3086 3089 3467 1186
Size: 1504 396 3 378 4578
Fragments arranged by size:
4578 1504 396 378 3
BseYI C'CCAG_C
Cuts at: 2648 2722 3016 4021 6062 6558 2648
Size: 74 294 1005 2041 496 2949
Fragments arranged by size:
2949 2041 1005 496 294 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3581 1163
Size: 573 1845 4441
Fragments arranged by size:
4441 1845 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2572 4309 5070 6098 1540
Size: 275 186 571 1737 761 1028 2301
Fragments arranged by size:
2301 1737 1028 761 571 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 2955 3459 3795 3882 4072 4920 5005 6076
Size: 264 504 336 87 190 848 85 1071
Cuts at: 6076 2691
Size: 3474
Fragments arranged by size:
3474 1071 848 504 336 264 190 87
85
BsmI GAATG_Cn'
Cuts at: 2893 3284 4370 4463 2893
Size: 391 1086 93 5289
Fragments arranged by size:
5289 1086 391 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 2417
Size: 6859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2141 2641 2691 2955 3459 3795
Size: 399 324 27 500 50 264 504 336
Cuts at: 3795 3882 4072 4178 4920 5005 6076 1391
Size: 87 190 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 500 399 336 324
264 190 106 87 85 50 27
BspEI T'CCGG_A
Cuts at: 6522 6522
Size: 6859
BspHI T'CATG_A
Cuts at: 2881 4748 6478 2881
Size: 1867 1730 3262
Fragments arranged by size:
3262 1867 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3241 4214 844
Size: 2397 973 3489
Fragments arranged by size:
3489 2397 973
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 3753 4746 1394
Size: 429 467 1463 993 3507
Fragments arranged by size:
3507 1463 993 467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3148 4095 5354 5528 65
Size: 1183 1900 947 1259 174 1396
Fragments arranged by size:
1900 1396 1259 1183 947 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2371 2571 5503 5690 1700
Size: 110 561 200 2932 187 2869
Fragments arranged by size:
2932 2869 561 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6859
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6859
BssSI C'ACGA_G
Cuts at: 2747 3652 4919 5931 2747
Size: 905 1267 1012 3675
Fragments arranged by size:
3675 1267 1012 905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 794
Size: 1532 5327
BstBI TT'CG_AA
Cuts at: 5663 5663
Size: 6859
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6859
BstXI CCAn_nnnn'nTGG
Cuts at: 3812 3812
Size: 6859
BstYI r'GATC_y
Cuts at: 1972 3205 3679 4189 4556 4941 4958 6410 6496
Size: 1233 474 510 367 385 17 1452 86
Cuts at: 6496 6508 6854 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1233 510 474 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2409 2436 513
Size: 552 939 405 27 4936
Fragments arranged by size:
4936 939 552 405 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6120
Fragments arranged by size:
6120 683 56
BtsI GCAGTG_nn'
Cuts at: 2394 2789 2891 4375 5169 5189 2394
Size: 395 102 1484 794 20 4064
Fragments arranged by size:
4064 1484 794 395 102 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2516 2551 529
Size: 35 1952 35 4837
Fragments arranged by size:
4837 1952 35 35
DraI TTT'AAA
Cuts at: 4163 4163
Size: 6859
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5866 809
Size: 507 4550 1802
Fragments arranged by size:
4550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2448 2524 2616
Size: 54 1425 50 275 18 618 76 92
Cuts at: 2616 3748 4306 5195 6824 8
Size: 1132 558 889 1629 43
Fragments arranged by size:
1629 1425 1132 889 618 558 275 92
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4306 1537
Size: 275 2494 4090
Fragments arranged by size:
4090 2494 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 3063 3504 4789 1353
Size: 1062 648 441 1285 3423
Fragments arranged by size:
3423 1285 1062 648 441
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 2354 2432 2593 2912 5446
Size: 1064 138 501 264 78 161 319 2534
Cuts at: 5446 5820 5966 387
Size: 374 146 1280
Fragments arranged by size:
2534 1280 1064 501 374 319 264 161
146 138 78
EcoICRI GAG'CTC
Cuts at: 2953 3457 3880 4070 2953
Size: 504 423 190 5742
Fragments arranged by size:
5742 504 423 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2969 3506 3769 4922 5519 6306
Size: 393 704 633 537 263 1153 597 787
Cuts at: 6306 6574 1239
Size: 268 1524
Fragments arranged by size:
1524 1153 787 704 633 597 537 393
268 263
EcoNI CCTnn'n_nnAGG
Cuts at: 3687 3687
Size: 6859
EcoO109I rG'GnC_Cy
Cuts at: 2111 2137 2138 2187 4095 2111
Size: 26 1 49 1908 4875
Fragments arranged by size:
4875 1908 49 26 1
EcoRI G'AATT_C
Cuts at: 4168 4546 4168
Size: 378 6481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3419 3799 3419
Size: 380 6479
FspI TGC'GCA
Cuts at: 5365 5365
Size: 6859
HaeII r_GCGC'y
Cuts at: 1678 2366 2372 2378 2578 2776 3784 6006 1678
Size: 688 6 6 200 198 1008 2222 2531
Fragments arranged by size:
2531 2222 1008 688 200 198 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 3077 3109 3669
Size: 32 135 32 923 32 550 32 560
Cuts at: 3669 3701 3737 3769 3949 3981 5500 5532 5574
Size: 32 36 32 180 32 1519 32 42
Cuts at: 5574 5606 1373
Size: 32 2626
Fragments arranged by size:
2626 1519 923 560 550 180 135 42
36 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2680 3763 4203 4449 669
Size: 988 281 742 1083 440 246 3079
Fragments arranged by size:
3079 1083 988 742 440 281 246
HindIII A'AGCT_T
Cuts at: 748 3500 748
Size: 2752 4107
HpaI GTT'AAC
Cuts at: 4449 4449
Size: 6859
KasI G'GCGC_C
Cuts at: 2368 2374 2368
Size: 6 6853
KpnI G_GTAC'C
Cuts at: 4184 4184
Size: 6859
MfeI C'AATT_G
Cuts at: 4458 4458
Size: 6859
MluI A'CGCG_T
Cuts at: 6735 6735
Size: 6859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3063 5973 6157 1305
Size: 1758 2910 184 2007
Fragments arranged by size:
2910 2007 1758 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2526 2618 10
Size: 54 1425 343 694 92 4251
Fragments arranged by size:
4251 1425 694 343 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 2886 3897 4817 5176 5335 518
Size: 648 1290 430 1011 920 359 159 2042
Fragments arranged by size:
2042 1290 1011 920 648 430 359 159
MspA1I CmG'CkG
Cuts at: 1606 2093 2299 2314 2323 2438 2671 2694 3808
Size: 487 206 15 9 115 233 23 1114
Cuts at: 3808 4275 4952 6100 6345 1606
Size: 467 677 1148 245 2120
Fragments arranged by size:
2120 1148 1114 677 487 467 245 233
206 115 23 15 9
NaeI GCC'GGC
Cuts at: 2373 2373
Size: 6859
NarI GG'CG_CC
Cuts at: 2369 2375 2369
Size: 6 6853
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5368
Fragments arranged by size:
5368 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6859
NgoMIV G'CCGG_C
Cuts at: 2371 2371
Size: 6859
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6859
NotI GC'GGCC_GC
Cuts at: 4306 4306
Size: 6859
NsiI A_TGCA'T
Cuts at: 3641 3641
Size: 6859
NspI r_CATG'y
Cuts at: 1132 1229 2916 3670 4217 5762 1132
Size: 97 1687 754 547 1545 2229
Fragments arranged by size:
2229 1687 1545 754 547 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6859
PciI A'CATG_T
Cuts at: 5758 5758
Size: 6859
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6859
PmeI GTTT'AAAC
Cuts at: 4163 4163
Size: 6859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4928 4960 6441 6473 4928
Size: 32 1481 32 5314
Fragments arranged by size:
5314 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2187 4095 2187
Size: 1908 4951
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6859
PsiI TTA'TAA
Cuts at: 4429 4429
Size: 6859
PspOMI G'GGCC_C
Cuts at: 2110 2137 2110
Size: 27 6832
PspXI vC'TCGA_Gb
Cuts at: 2264 2264
Size: 6859
PstI C_TGCA'G
Cuts at: 830 2134 4211 830
Size: 1304 2077 3478
Fragments arranged by size:
3478 2077 1304
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6859
PvuII CAG'CTG
Cuts at: 2299 2314 2323 2299
Size: 15 9 6835
Fragments arranged by size:
6835 15 9
SacI G_AGCT'C
Cuts at: 2955 3459 3882 4072 2955
Size: 504 423 190 5742
Fragments arranged by size:
5742 504 423 190
SacII CC_GC'GG
Cuts at: 2439 2439
Size: 6859
SalI G'TCGA_C
Cuts at: 1936 4201 1936
Size: 2265 4594
SbfI CC_TGCA'GG
Cuts at: 4211 4211
Size: 6859
ScaI AGT'ACT
Cuts at: 5107 6819 5107
Size: 1712 5147
SfcI C'TryA_G
Cuts at: 826 1045 2130 4207 5342 6023 6214 826
Size: 219 1085 2077 1135 681 191 1471
Fragments arranged by size:
2077 1471 1135 1085 681 219 191
SfoI GGC'GCC
Cuts at: 2370 2376 2370
Size: 6 6853
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
SgrAI Cr'CCGG_yG
Cuts at: 1700 2371 1700
Size: 671 6188
SmaI CCC'GGG
Cuts at: 1872 2243 4186 1872
Size: 371 1943 4545
Fragments arranged by size:
4545 1943 371
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2795 4963 5864 6126 820
Size: 197 926 321 531 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 531 321 262 197
SnaBI TAC'GTA
Cuts at: 493 4650 493
Size: 4157 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6859
SphI G_CATG'C
Cuts at: 1132 4217 1132
Size: 3085 3774
SspI AAT'ATT
Cuts at: 5 52 4783 5
Size: 47 4731 2081
Fragments arranged by size:
4731 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6859
StyI C'CwwG_G
Cuts at: 513 1065 2004 2232 2871 3673 4056 4298 513
Size: 552 939 228 639 802 383 242 3074
Fragments arranged by size:
3074 939 802 639 552 383 242 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2133 2841 4886 4903 5056 5241 5686
Size: 1255 553 708 2045 17 153 185 445
Cuts at: 5686 325
Size: 1498
Fragments arranged by size:
2045 1498 1255 708 553 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 3563 5105
Size: 274 80 33 51 1443 822 764 1542
Cuts at: 5105 6817 96
Size: 1712 138
Fragments arranged by size:
1712 1542 1443 822 764 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6819 6851 6819
Size: 32 6827
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6859
XbaI T'CTAG_A
Cuts at: 4195 4195
Size: 6859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4029 4029
Size: 6859
XhoI C'TCGA_G
Cuts at: 1943 2264 1943
Size: 321 6538
XmaI C'CCGG_G
Cuts at: 1870 2241 4184 1870
Size: 371 1943 4545
Fragments arranged by size:
4545 1943 371
XmnI GAAnn'nnTTC
Cuts at: 4988 4988
Size: 6859
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4667 276
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI
PacI PfoI PmlI PsrI RsrII SanDI SapI SexAI
SfiI SrfI SwaI