Restriction map for
pFN21AE4214
(Circular) (Six-base) MAPSORT of: pFN21AE4214.seq Check: 4739 from: 1 to: 6910
pFN21AE4214 6910 bp
With 182 enzymes: SgfI *
March 28, 2012 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3618 3888 3618
Size: 270 6640
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4720 278
Size: 53 83 186 936 3184 2468
Fragments arranged by size:
3184 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4253 1937
Size: 2316 4594
Acc65I G'GTAC_C
Cuts at: 2302 4231 2302
Size: 1929 4981
AclI AA'CG_TT
Cuts at: 1563 5037 5410 1563
Size: 3474 373 3063
Fragments arranged by size:
3474 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4973 6357 6625 1632
Size: 3341 1384 268 1917
Fragments arranged by size:
3341 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6713
AflIII A'CryG_T
Cuts at: 1203 4200 5809 6786 1203
Size: 2997 1609 977 1327
Fragments arranged by size:
2997 1609 1327 977
AgeI A'CCGG_T
Cuts at: 4091 5741 4091
Size: 1650 5260
AhdI GACnn_n'nnGTC
Cuts at: 5639 5639
Size: 6910
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3074 3394 1184
Size: 1890 320 4700
Fragments arranged by size:
4700 1890 320
AlwNI CAG_nnn'CTG
Cuts at: 2378 2660 3124 3475 4653 6225 2378
Size: 282 464 351 1178 1572 3063
Fragments arranged by size:
3063 1572 1178 464 351 282
ApaI G_GGCC'C
Cuts at: 2178 2489 2642 2730 2908 2178
Size: 311 153 88 178 6180
Fragments arranged by size:
6180 311 178 153 88
ApaLI G'TGCA_C
Cuts at: 4967 6123 4967
Size: 1156 5754
ApoI r'AATT_y
Cuts at: 1493 4219 4445 4597 6706 1493
Size: 2726 226 152 2109 1697
Fragments arranged by size:
2726 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5464 160
Size: 872 4432 1606
Fragments arranged by size:
4432 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
AvaI C'yCGr_G
Cuts at: 1870 1943 2186 2386 2482 2559 2642 2677 2775
Size: 73 243 200 96 77 83 35 98
Cuts at: 2775 2828 3504 3546 3943 4235 1870
Size: 53 676 42 397 292 4545
Fragments arranged by size:
4545 676 397 292 243 200 98 96
83 77 73 53 42 35
BamHI G'GATC_C
Cuts at: 4240 4607 4240
Size: 367 6543
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 2496 2535 2666 2779
Size: 325 217 627 515 194 39 131 113
Cuts at: 2779 3030 3050 3081 3241 3542 3703 4231 5686
Size: 251 20 31 160 301 161 528 1455
Cuts at: 5686 618
Size: 1842
Fragments arranged by size:
1842 1455 627 528 515 325 301 251
217 194 161 160 131 113 39 31
20
BanII G_rGCy'C
Cuts at: 1391 2118 2139 2178 2204 2238 2322 2385 2489
Size: 727 21 39 26 34 84 63 104
Cuts at: 2489 2600 2642 2678 2730 2908 3183 3555 4185
Size: 111 42 36 52 178 275 372 630
Cuts at: 4185 4229 1391
Size: 44 4072
Fragments arranged by size:
4072 727 630 372 275 178 111 104
84 63 52 44 42 39 36 34
26 21
BbeI G_GCGC'C
Cuts at: 2500 2539 3707 2500
Size: 39 1168 5703
Fragments arranged by size:
5703 1168 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 3064 4110 928
Size: 971 465 700 1046 3728
Fragments arranged by size:
3728 1046 971 700 465
BbvCI CC'TCA_GC
Cuts at: 2277 2693 2807 3557 2277
Size: 416 114 750 5630
Fragments arranged by size:
5630 750 416 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2567 2601 4097 4131 5101 5135 1903
Size: 34 630 34 1496 34 970 34 3678
Fragments arranged by size:
3678 1496 970 630 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4802 6012 4802
Size: 1210 5700
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6910
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2046 2389 2553
Size: 107 122 71 631 605 374 343 164
Cuts at: 2553 3121 3712 4000 5521 136
Size: 568 591 288 1521 1525
Fragments arranged by size:
1525 1521 631 605 591 568 374 343
288 164 122 107 71
BglII A'GATC_T
Cuts at: 2331 6905 2331
Size: 4574 2336
BlpI GC'TnA_GC
Cuts at: 2404 3177 3322 4327 2404
Size: 773 145 1005 4987
Fragments arranged by size:
4987 1005 773 145
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2489 2642 2669 2730 2782 2908 3046
Size: 388 311 153 27 61 52 126 138
Cuts at: 3046 3053 3246 3545 3724 4971 6127 1790
Size: 7 193 299 179 1247 1156 2573
Fragments arranged by size:
2573 1247 1156 388 311 299 193 179
153 138 126 61 52 27 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6910
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2232 2322 4641
Size: 416 53 83 399 24 815 90 2319
Cuts at: 4641 5599 442
Size: 958 1753
Fragments arranged by size:
2319 1753 958 815 416 399 90 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6910
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2194 2302 3572 2194
Size: 108 1270 5532
Fragments arranged by size:
5532 1270 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2164 2252 2355 2987 2993 3131 3512
Size: 870 55 88 103 632 6 138 381
Cuts at: 3512 3816 3988 4193 5570 1239
Size: 304 172 205 1377 2579
Fragments arranged by size:
2579 1377 870 632 381 304 205 172
138 103 88 55 6
Bpu10I CC'TnA_GC
Cuts at: 2277 2379 2543 2693 2807 3557 2277
Size: 102 164 150 114 750 5630
Fragments arranged by size:
5630 750 164 150 114 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3413 5035 5900 6198 3413
Size: 1622 865 298 4125
Fragments arranged by size:
4125 1622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2831 5573 882
Size: 617 1332 2742 2219
Fragments arranged by size:
2742 2219 1332 617
BsaAI yAC'GTr
Cuts at: 493 1204 4036 4701 493
Size: 711 2832 665 2702
Fragments arranged by size:
2832 2702 711 665
BsaBI GATnn'nnATC
Cuts at: 6904 6904
Size: 6910
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2497 2536 3704
Size: 53 83 186 936 546 418 39 1168
Cuts at: 3704 4155 4717 5099 5734 275
Size: 451 562 382 635 1451
Fragments arranged by size:
1451 1168 936 635 562 546 451 418
382 186 83 53 39
BsaWI w'CCGG_w
Cuts at: 2710 4091 5343 5741 6015 6162 6573 2710
Size: 1381 1252 398 274 147 411 3047
Fragments arranged by size:
3047 1381 1252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6685
Fragments arranged by size:
6685 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3545 3613 3939 4082 1186
Size: 2359 68 326 143 4014
Fragments arranged by size:
4014 2359 326 143 68
BseYI C'CCAG_C
Cuts at: 2098 2103 2117 2257 2719 2723 3468 6113 6609
Size: 5 14 140 462 4 745 2645 496
Cuts at: 6609 2098
Size: 2399
Fragments arranged by size:
2645 2399 745 496 462 140 14 5
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2190 2457 2798 3390 3767 3771 3860
Size: 573 454 267 341 592 377 4 89
Cuts at: 3860 1163
Size: 4213
Fragments arranged by size:
4213 592 573 454 377 341 267 89
4
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2790 3922 4360 5121 6149 1540
Size: 275 186 789 1132 438 761 1028 2301
Fragments arranged by size:
2301 1132 1028 789 761 438 275 186
BsiHKAI G_wGCw'C
Cuts at: 4185 4971 5056 6127 4185
Size: 786 85 1071 4968
Fragments arranged by size:
4968 1071 786 85
BsmI GAATG_Cn'
Cuts at: 4421 4514 4421
Size: 93 6817
BsmBI CGTCTCn'nnnn_
Cuts at: 3625 3625
Size: 6910
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2139 2178 2204 2238 2322 2385
Size: 399 328 21 39 26 34 84 63
Cuts at: 2385 2489 2600 2642 2669 2678 2730 2782 2908
Size: 104 111 42 27 9 52 52 126
Cuts at: 2908 3046 3053 3183 3246 3545 3555 3724 4185
Size: 138 7 130 63 299 10 169 461
Cuts at: 4185 4229 4971 5056 6127 1391
Size: 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 461 399 328 299 169
138 130 126 111 104 85 84 63
63 52 52 44 42 39 34 27
26 21 10 9 7
BspEI T'CCGG_A
Cuts at: 6573 6573
Size: 6910
BspHI T'CATG_A
Cuts at: 4799 6529 4799
Size: 1730 5180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 3618 3798 3888 4265 844
Size: 1385 1389 180 90 377 3489
Fragments arranged by size:
3489 1389 1385 377 180 90
BsrBI CCG'CTC
Cuts at: 1394 1823 3220 3226 3361 4797 1394
Size: 429 1397 6 135 1436 3507
Fragments arranged by size:
3507 1436 1397 429 135 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 5405 5579 65
Size: 1183 4157 174 1396
Fragments arranged by size:
4157 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2324 2647 2684 3033 3691 3959 4091
Size: 110 514 323 37 349 658 268 132
Cuts at: 4091 5554 5741 1700
Size: 1463 187 2869
Fragments arranged by size:
2869 1463 658 514 349 323 268 187
132 110 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6910
BssHII G'CGCG_C
Cuts at: 1925 2992 1925
Size: 1067 5843
BssSI C'ACGA_G
Cuts at: 3717 4970 5982 3717
Size: 1253 1012 4645
Fragments arranged by size:
4645 1253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3790 794
Size: 2996 3914
BstBI TT'CG_AA
Cuts at: 4101 5714 4101
Size: 1613 5297
BstEII G'GTnAC_C
Cuts at: 1184 2687 4087 1184
Size: 1503 1400 4007
Fragments arranged by size:
4007 1503 1400
BstXI CCAn_nnnn'nTGG
Cuts at: 3754 3754
Size: 6910
BstYI r'GATC_y
Cuts at: 1972 2331 3808 4168 4240 4607 4992 5009 6461
Size: 359 1477 360 72 367 385 17 1452
Cuts at: 6461 6547 6559 6905 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1477 1452 385 367 360 359 346
86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 2352 3117 2352
Size: 765 6145
BtgI C'CryG_G
Cuts at: 513 1065 2004 3066 3498 3916 513
Size: 552 939 1062 432 418 3507
Fragments arranged by size:
3507 1062 939 552 432 418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6171
Fragments arranged by size:
6171 683 56
BtsI GCAGTG_nn'
Cuts at: 2918 3206 3336 3759 4051 4426 5220 5240 2918
Size: 288 130 423 292 375 794 20 4588
Fragments arranged by size:
4588 794 423 375 292 288 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6910
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3642 3677 529
Size: 35 3078 35 3762
Fragments arranged by size:
3762 3078 35 35
DraI TTT'AAA
Cuts at: 4214 4214
Size: 6910
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3309 4070 5917 809
Size: 507 1993 761 1847 1802
Fragments arranged by size:
1993 1847 1802 761 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2092 2787
Size: 54 1425 50 275 18 177 85 695
Cuts at: 2787 2840 3099 3235 3663 3803 4357 5246 6875
Size: 53 259 136 428 140 554 889 1629
Cuts at: 6875 8
Size: 43
Fragments arranged by size:
1629 1425 889 695 554 428 275 259
177 140 136 85 54 53 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2787 4357 1537
Size: 275 975 1570 4090
Fragments arranged by size:
4090 1570 975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4840 1353
Size: 3487 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5497 5871 6017 387
Size: 1064 138 3908 374 146 1280
Fragments arranged by size:
3908 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4183 4183
Size: 6910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2164 2252 2355 2987 2993 3131
Size: 393 477 55 88 103 632 6 138
Cuts at: 3131 3512 3816 3988 4193 4973 5570 6357 6625
Size: 381 304 172 205 780 597 787 268
Cuts at: 6625 1239
Size: 1524
Fragments arranged by size:
1524 787 780 632 597 477 393 381
304 268 205 172 138 103 88 55
6
EcoNI CCTnn'n_nnAGG
Cuts at: 3386 3479 3386
Size: 93 6817
EcoO109I rG'GnC_Cy
Cuts at: 2154 2174 2175 2294 2419 2486 2555 2638 2639
Size: 20 1 119 125 67 69 83 1
Cuts at: 2639 2657 2727 2765 2866 2904 3013 3391 3508
Size: 18 70 38 101 38 109 378 117
Cuts at: 3508 3929 2154
Size: 421 5135
Fragments arranged by size:
5135 421 378 125 119 117 109 101
83 70 69 67 38 38 20 18
1 1
EcoRI G'AATT_C
Cuts at: 4219 4597 4219
Size: 378 6532
EcoRV GAT'ATC
Cuts at: 3406 3406
Size: 6910
FspI TGC'GCA
Cuts at: 5416 5416
Size: 6910
HaeII r_GCGC'y
Cuts at: 1678 2110 2500 2539 2719 2849 3283 3707 6057
Size: 432 390 39 180 130 434 424 2350
Cuts at: 6057 1678
Size: 2531
Fragments arranged by size:
2531 2350 434 432 424 390 180 130
39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2690 2722 3998 4030 5551
Size: 32 135 32 1118 32 1276 32 1521
Cuts at: 5551 5583 5625 5657 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1521 1276 1118 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4254 4500 669
Size: 988 281 2316 246 3079
Fragments arranged by size:
3079 2316 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6910
HpaI GTT'AAC
Cuts at: 4500 4500
Size: 6910
KasI G'GCGC_C
Cuts at: 2496 2535 3703 2496
Size: 39 1168 5703
Fragments arranged by size:
5703 1168 39
KpnI G_GTAC'C
Cuts at: 2306 4235 2306
Size: 1929 4981
MfeI C'AATT_G
Cuts at: 4509 4509
Size: 6910
MluI A'CGCG_T
Cuts at: 6786 6786
Size: 6910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 3129 6024 6208 1305
Size: 750 1074 2895 184 2007
Fragments arranged by size:
2895 2007 1074 750 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3805 10
Size: 54 1425 343 1973 3115
Fragments arranged by size:
3115 1973 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 3776 4868 5227 5386 518
Size: 648 1344 1266 1092 359 159 2042
Fragments arranged by size:
2042 1344 1266 1092 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2276 2408 2723 3097 3176 3326 3374 3561
Size: 670 132 315 374 79 150 48 187
Cuts at: 3561 3886 3918 4326 5003 6151 6396 1606
Size: 325 32 408 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 670 408 374 325 315
245 187 150 132 79 48 32
NaeI GCC'GGC
Cuts at: 2326 2649 3693 3961 2326
Size: 323 1044 268 5275
Fragments arranged by size:
5275 1044 323 268
NarI GG'CG_CC
Cuts at: 2497 2536 3704 2497
Size: 39 1168 5703
Fragments arranged by size:
5703 1168 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 3498 513
Size: 552 939 1494 3925
Fragments arranged by size:
3925 1494 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6910
NgoMIV G'CCGG_C
Cuts at: 2324 2647 3691 3959 2324
Size: 323 1044 268 5275
Fragments arranged by size:
5275 1044 323 268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6910
NotI GC'GGCC_GC
Cuts at: 4357 4357
Size: 6910
NspI r_CATG'y
Cuts at: 1132 1229 4268 5813 1132
Size: 97 3039 1545 2229
Fragments arranged by size:
3039 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2862 3046 1348
Size: 1514 184 5212
Fragments arranged by size:
5212 1514 184
PciI A'CATG_T
Cuts at: 5809 5809
Size: 6910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2399 3097 1108
Size: 1291 698 4921
Fragments arranged by size:
4921 1291 698
PfoI T'CCnGG_A
Cuts at: 3600 4164 3600
Size: 564 6346
PmeI GTTT'AAAC
Cuts at: 4214 4214
Size: 6910
PmlI CAC'GTG
Cuts at: 4036 4036
Size: 6910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4979 5011 6492 6524 4979
Size: 32 1481 32 5365
Fragments arranged by size:
5365 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2154 2765 2866 3013 3391 3508 2154
Size: 611 101 147 378 117 5556
Fragments arranged by size:
5556 611 378 147 117 101
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6910
PsiI TTA'TAA
Cuts at: 4480 4480
Size: 6910
PspOMI G'GGCC_C
Cuts at: 2174 2485 2638 2726 2904 2174
Size: 311 153 88 178 6180
Fragments arranged by size:
6180 311 178 153 88
PstI C_TGCA'G
Cuts at: 830 3798 4262 830
Size: 2968 464 3478
Fragments arranged by size:
3478 2968 464
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6910
PvuII CAG'CTG
Cuts at: 2276 2408 2723 3097 3176 3561 2276
Size: 132 315 374 79 385 5625
Fragments arranged by size:
5625 385 374 315 132 79
SacI G_AGCT'C
Cuts at: 4185 4185
Size: 6910
SacII CC_GC'GG
Cuts at: 3919 3919
Size: 6910
SalI G'TCGA_C
Cuts at: 1936 4252 1936
Size: 2316 4594
SanDI GG'GwC_CC
Cuts at: 2154 2765 3013 3508 2154
Size: 611 248 495 5556
Fragments arranged by size:
5556 611 495 248
SbfI CC_TGCA'GG
Cuts at: 4262 4262
Size: 6910
ScaI AGT'ACT
Cuts at: 5158 6870 5158
Size: 1712 5198
SfcI C'TryA_G
Cuts at: 826 1045 3794 4258 5393 6074 6265 826
Size: 219 2749 464 1135 681 191 1471
Fragments arranged by size:
2749 1471 1135 681 464 219 191
SfoI GGC'GCC
Cuts at: 2498 2537 3705 2498
Size: 39 1168 5703
Fragments arranged by size:
5703 1168 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
SgrAI Cr'CCGG_yG
Cuts at: 1700 2324 3959 4091 1700
Size: 624 1635 132 4519
Fragments arranged by size:
4519 1635 624 132
SmaI CCC'GGG
Cuts at: 1872 2388 2484 2561 2644 2777 3506 4237 1872
Size: 516 96 77 83 133 729 731 4545
Fragments arranged by size:
4545 731 729 516 133 96 83 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3428 5014 5915 6177 820
Size: 197 926 1485 1586 901 262 1553
Fragments arranged by size:
1586 1553 1485 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4701 493
Size: 4208 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6910
SphI G_CATG'C
Cuts at: 1132 4268 1132
Size: 3136 3774
SrfI GCCC'GGGC
Cuts at: 2777 2777
Size: 6910
SspI AAT'ATT
Cuts at: 5 52 4834 5
Size: 47 4782 2081
Fragments arranged by size:
4782 2081 47
StuI AGG'CCT
Cuts at: 1517 3715 3741 1517
Size: 2198 26 4686
Fragments arranged by size:
4686 2198 26
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2431 3498 4349 513
Size: 552 939 78 349 1067 851 3074
Fragments arranged by size:
3074 1067 939 851 552 349 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2112 2242 2249 2752 3482 4937 4954
Size: 1255 532 130 7 503 730 1455 17
Cuts at: 4954 5107 5292 5737 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1455 1255 730 532 503 445 185
153 130 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5156 6868 96
Size: 274 80 33 51 1443 3179 1712 138
Fragments arranged by size:
3179 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6870 6902 6870
Size: 32 6878
Tth111I GACn'n_nGTC
Cuts at: 1316 3145 1316
Size: 1829 5081
XbaI T'CTAG_A
Cuts at: 4246 4246
Size: 6910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3263 3263
Size: 6910
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6910
XmaI C'CCGG_G
Cuts at: 1870 2386 2482 2559 2642 2775 3504 4235 1870
Size: 516 96 77 83 133 729 731 4545
Fragments arranged by size:
4545 731 729 516 133 96 83 77
XmnI GAAnn'nnTTC
Cuts at: 2030 5039 2030
Size: 3009 3901
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4718 276
Size: 53 83 186 936 3184 2468
Fragments arranged by size:
3184 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SapI SexAI SfiI SwaI