Restriction map for
pFN21AE4209
(Circular) (Six-base) MAPSORT of: pFN21AE4209.seq Check: 7878 from: 1 to: 6892
pFN21AE4209 6892 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2329 4702 278
Size: 53 83 186 936 793 2373 2468
Fragments arranged by size:
2468 2373 936 793 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2113 2852 4235 1937
Size: 176 739 1383 4594
Fragments arranged by size:
4594 1383 739 176
Acc65I G'GTAC_C
Cuts at: 2490 4213 2490
Size: 1723 5169
AclI AA'CG_TT
Cuts at: 1563 5019 5392 1563
Size: 3456 373 3063
Fragments arranged by size:
3456 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 4053 4955 6339 6607 1632
Size: 1302 1119 902 1384 268 1917
Fragments arranged by size:
1917 1384 1302 1119 902 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6695
AflIII A'CryG_T
Cuts at: 1203 2847 3153 5791 6768 1203
Size: 1644 306 2638 977 1327
Fragments arranged by size:
2638 1644 1327 977 306
AgeI A'CCGG_T
Cuts at: 5723 5723
Size: 6892
AhdI GACnn_n'nnGTC
Cuts at: 5621 5621
Size: 6892
AleI CACnn'nnGTG
Cuts at: 1166 2981 3110 3602 4080 1166
Size: 1815 129 492 478 3978
Fragments arranged by size:
3978 1815 492 478 129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2999 1184
Size: 1815 5077
AlwNI CAG_nnn'CTG
Cuts at: 4635 6207 4635
Size: 1572 5320
ApaI G_GGCC'C
Cuts at: 2587 2870 4052 2587
Size: 283 1182 5427
Fragments arranged by size:
5427 1182 283
ApaLI G'TGCA_C
Cuts at: 4949 6105 4949
Size: 1156 5736
ApoI r'AATT_y
Cuts at: 1493 4201 4427 4579 6688 1493
Size: 2708 226 152 2109 1697
Fragments arranged by size:
2708 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5446 160
Size: 872 4414 1606
Fragments arranged by size:
4414 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 4217 1870
Size: 73 445 1829 4545
Fragments arranged by size:
4545 1829 445 73
BamHI G'GATC_C
Cuts at: 3563 3591 3678 3780 4222 4589 3563
Size: 28 87 102 442 367 5866
Fragments arranged by size:
5866 442 367 102 87 28
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2148 2383 2490 2887 2955
Size: 325 217 627 361 235 107 397 68
Cuts at: 2955 4213 5668 618
Size: 1258 1455 1842
Fragments arranged by size:
1842 1455 1258 627 397 361 325 235
217 107 68
BanII G_rGCy'C
Cuts at: 1391 2249 2264 2587 2870 3359 3744 4052 4211
Size: 858 15 323 283 489 385 308 159
Cuts at: 4211 1391
Size: 4072
Fragments arranged by size:
4072 858 489 385 323 308 283 159
15
BbeI G_GCGC'C
Cuts at: 2959 2959
Size: 6892
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3819 928
Size: 971 1920 4001
Fragments arranged by size:
4001 1920 971
BbvCI CC'TCA_GC
Cuts at: 3648 3694 3648
Size: 46 6846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2272 2306 4068 4102 5083 5117 1903
Size: 34 335 34 1762 34 981 34 3678
Fragments arranged by size:
3678 1762 981 335 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4784 5994 4784
Size: 1210 5682
BclI T'GATC_A
Cuts at: 1548 2670 3012 1548
Size: 1122 342 5428
Fragments arranged by size:
5428 1122 342
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3249 5503 136
Size: 107 122 71 631 605 1577 2254 1525
Fragments arranged by size:
2254 1577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6887 6887
Size: 6892
BlpI GC'TnA_GC
Cuts at: 4309 4309
Size: 6892
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2153 2587 2870 3468 4052 4953 6109
Size: 252 111 434 283 598 584 901 1156
Cuts at: 6109 1790
Size: 2573
Fragments arranged by size:
2573 1156 901 598 584 434 283 252
111
BmgBI CAC'GTC
Cuts at: 1318 2850 1318
Size: 1532 5360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 2975 4623
Size: 416 53 83 399 24 1284 274 1648
Cuts at: 4623 5581 442
Size: 958 1753
Fragments arranged by size:
1753 1648 1284 958 416 399 274 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6892
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3858 3858
Size: 6892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2821 2980 3078 3566 5552 1239
Size: 815 767 159 98 488 1986 2579
Fragments arranged by size:
2579 1986 815 767 488 159 98
Bpu10I CC'TnA_GC
Cuts at: 2236 3257 3648 3694 2236
Size: 1021 391 46 5434
Fragments arranged by size:
5434 1021 391 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 3883 4001 5017 5882 6180 2633
Size: 1250 118 1016 865 298 3345
Fragments arranged by size:
3345 1250 1016 865 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3368 3531 3568 5555 882
Size: 617 1869 163 37 1987 2219
Fragments arranged by size:
2219 1987 1869 617 163 37
BsaAI yAC'GTr
Cuts at: 493 1204 3753 4683 493
Size: 711 2549 930 2702
Fragments arranged by size:
2702 2549 930 711
BsaBI GATnn'nnATC
Cuts at: 6886 6886
Size: 6892
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2165 2326 2956 3222
Size: 53 83 186 936 632 161 630 266
Cuts at: 3222 3264 4022 4699 5081 5716 275
Size: 42 758 677 382 635 1451
Fragments arranged by size:
1451 936 758 677 635 632 630 382
266 186 161 83 53 42
BsaWI w'CCGG_w
Cuts at: 2466 2931 3989 4094 4113 5325 5723 5997 6144
Size: 465 1058 105 19 1212 398 274 147
Cuts at: 6144 6555 2466
Size: 411 2803
Fragments arranged by size:
2803 1212 1058 465 411 398 274 147
105 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2703 2733 3207 3237 3346
Size: 30 165 30 1300 30 474 30 109
Cuts at: 3346 3376 3701 3731 1178
Size: 30 325 30 4339
Fragments arranged by size:
4339 1300 474 325 165 109 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3382 3676 3945 1186
Size: 2196 294 269 4133
Fragments arranged by size:
4133 2196 294 269
BseYI C'CCAG_C
Cuts at: 2527 2562 3018 3132 4026 4184 6095 6591 2527
Size: 35 456 114 894 158 1911 496 2828
Fragments arranged by size:
2828 1911 894 496 456 158 114 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2788 3152 1163
Size: 573 1052 364 4903
Fragments arranged by size:
4903 1052 573 364
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4342 5103 6131 1540
Size: 275 186 2341 761 1028 2301
Fragments arranged by size:
2341 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2249 2988 4953 5038 6109 2249
Size: 739 1965 85 1071 3032
Fragments arranged by size:
3032 1965 1071 739 85
BsmI GAATG_Cn'
Cuts at: 2162 4403 4496 2162
Size: 2241 93 4558
Fragments arranged by size:
4558 2241 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3271 3271
Size: 6892
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2153 2249 2264 2587 2870 2988
Size: 399 252 111 96 15 323 283 118
Cuts at: 2988 3359 3468 3744 4052 4211 4953 5038 6109
Size: 371 109 276 308 159 742 85 1071
Cuts at: 6109 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 371 323 308 283
276 252 159 118 111 109 96 85
15
BspEI T'CCGG_A
Cuts at: 2466 3989 4094 4113 6555 2466
Size: 1523 105 19 2442 2803
Fragments arranged by size:
2803 2442 1523 105 19
BspHI T'CATG_A
Cuts at: 4781 6511 4781
Size: 1730 5162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 3213 4247 844
Size: 1367 1002 1034 3489
Fragments arranged by size:
3489 1367 1034 1002
BsrBI CCG'CTC
Cuts at: 1394 1823 2575 4779 1394
Size: 429 752 2204 3507
Fragments arranged by size:
3507 2204 752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5387 5561 65
Size: 1183 4139 174 1396
Fragments arranged by size:
4139 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2194 3500 5536 5723 1700
Size: 110 384 1306 2036 187 2869
Fragments arranged by size:
2869 2036 1306 384 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6892
BssSI C'ACGA_G
Cuts at: 2210 3602 4952 5964 2210
Size: 1392 1350 1012 3138
Fragments arranged by size:
3138 1392 1350 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6892
BstBI TT'CG_AA
Cuts at: 5696 5696
Size: 6892
BstEII G'GTnAC_C
Cuts at: 1184 2429 3274 1184
Size: 1245 845 4802
Fragments arranged by size:
4802 1245 845
BstXI CCAn_nnnn'nTGG
Cuts at: 2378 2539 3055 3112 3604 4082 4147 2378
Size: 161 516 57 492 478 65 5123
Fragments arranged by size:
5123 516 492 478 161 65 57
BstYI r'GATC_y
Cuts at: 1972 3563 3591 3678 3780 4222 4589 4974 4991
Size: 1591 28 87 102 442 367 385 17
Cuts at: 4991 6443 6529 6541 6887 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1591 1452 442 385 367 346 102
87 86 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 2435 4162 2435
Size: 1727 5165
BtgI C'CryG_G
Cuts at: 513 1065 2004 2538 3024 3111 3184 3431 3633
Size: 552 939 534 486 87 73 247 202
Cuts at: 3633 3699 3789 513
Size: 66 90 3616
Fragments arranged by size:
3616 939 552 534 486 247 202 90
87 73 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6153
Fragments arranged by size:
6153 683 56
BtsI GCAGTG_nn'
Cuts at: 3468 3775 4155 4408 5202 5222 3468
Size: 307 380 253 794 20 5138
Fragments arranged by size:
5138 794 380 307 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6892
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6857
DraI TTT'AAA
Cuts at: 4196 4196
Size: 6892
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5899 809
Size: 507 4583 1802
Fragments arranged by size:
4583 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 3792 4339
Size: 54 1425 50 275 18 366 1596 547
Cuts at: 4339 5228 6857 8
Size: 889 1629 43
Fragments arranged by size:
1629 1596 1425 889 547 366 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4339 1537
Size: 275 2527 4090
Fragments arranged by size:
4090 2527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3534 4822 1353
Size: 2181 1288 3423
Fragments arranged by size:
3423 2181 1288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3556 5479 5853 5999 387
Size: 1064 138 1967 1923 374 146 1280
Fragments arranged by size:
1967 1923 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2247 2247
Size: 6892
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2821 2934 2980 3078 3566 4053
Size: 393 422 767 113 46 98 488 487
Cuts at: 4053 4955 5552 6339 6607 1239
Size: 902 597 787 268 1524
Fragments arranged by size:
1524 902 787 767 597 488 487 422
393 268 113 98 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2269 3209 2269
Size: 940 5952
EcoO109I rG'GnC_Cy
Cuts at: 2583 2584 2866 3229 2583
Size: 1 282 363 6246
Fragments arranged by size:
6246 363 282 1
EcoRI G'AATT_C
Cuts at: 4201 4579 4201
Size: 378 6514
EcoRV GAT'ATC
Cuts at: 2455 2659 2455
Size: 204 6688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3545 3545
Size: 6892
FspI TGC'GCA
Cuts at: 5398 5398
Size: 6892
HaeII r_GCGC'y
Cuts at: 1678 2192 2959 6039 1678
Size: 514 767 3080 2531
Fragments arranged by size:
3080 2531 767 514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2442 2474 3207 3239 3276
Size: 32 135 32 870 32 733 32 37
Cuts at: 3276 3308 3346 3378 3583 3615 3701 3733 5533
Size: 32 38 32 205 32 86 32 1800
Cuts at: 5533 5565 5607 5639 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1800 870 733 205 135 86 42
38 37 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3481 4236 4482 669
Size: 988 281 1543 755 246 3079
Fragments arranged by size:
3079 1543 988 755 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6892
HpaI GTT'AAC
Cuts at: 4482 4482
Size: 6892
KasI G'GCGC_C
Cuts at: 2955 2955
Size: 6892
KpnI G_GTAC'C
Cuts at: 2494 4217 2494
Size: 1723 5169
MfeI C'AATT_G
Cuts at: 4491 4491
Size: 6892
MluI A'CGCG_T
Cuts at: 6768 6768
Size: 6892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 6006 6190 1305
Size: 897 3804 184 2007
Fragments arranged by size:
3804 2007 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5070
Fragments arranged by size:
5070 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2376 2666 2981 3110 3602 3881 4080
Size: 648 1210 290 315 129 492 279 199
Cuts at: 4080 4850 5209 5368 518
Size: 770 359 159 2042
Fragments arranged by size:
2042 1210 770 648 492 359 315 290
279 199 159 129
MspA1I CmG'CkG
Cuts at: 1606 2517 2557 3051 3791 4308 4985 6133 6378
Size: 911 40 494 740 517 677 1148 245
Cuts at: 6378 1606
Size: 2120
Fragments arranged by size:
2120 1148 911 740 677 517 494 245
40
NaeI GCC'GGC
Cuts at: 2196 3502 2196
Size: 1306 5586
NarI GG'CG_CC
Cuts at: 2956 2956
Size: 6892
NcoI C'CATG_G
Cuts at: 513 1065 2004 3024 3184 3633 513
Size: 552 939 1020 160 449 3772
Fragments arranged by size:
3772 1020 939 552 449 160
NdeI CA'TA_TG
Cuts at: 387 2961 387
Size: 2574 4318
NgoMIV G'CCGG_C
Cuts at: 2194 3500 2194
Size: 1306 5586
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6892
NotI GC'GGCC_GC
Cuts at: 4339 4339
Size: 6892
NspI r_CATG'y
Cuts at: 1132 1229 3157 4250 5795 1132
Size: 97 1928 1093 1545 2229
Fragments arranged by size:
2229 1928 1545 1093 97
PasI CC'CwG_GG
Cuts at: 1348 2870 3506 1348
Size: 1522 636 4734
Fragments arranged by size:
4734 1522 636
PciI A'CATG_T
Cuts at: 3153 5791 3153
Size: 2638 4254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3025 1108
Size: 1917 4975
PfoI T'CCnGG_A
Cuts at: 2249 2876 3094 3350 3587 2249
Size: 627 218 256 237 5554
Fragments arranged by size:
5554 627 256 237 218
PmeI GTTT'AAAC
Cuts at: 4196 4196
Size: 6892
PmlI CAC'GTG
Cuts at: 3753 3753
Size: 6892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4961 4993 6474 6506 4961
Size: 32 1481 32 5347
Fragments arranged by size:
5347 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3229 3229
Size: 6892
PshAI GACnn'nnGTC
Cuts at: 1613 3523 1613
Size: 1910 4982
PsiI TTA'TAA
Cuts at: 4462 4462
Size: 6892
PspOMI G'GGCC_C
Cuts at: 2583 2866 4048 2583
Size: 283 1182 5427
Fragments arranged by size:
5427 1182 283
PspXI vC'TCGA_Gb
Cuts at: 2388 2388
Size: 6892
PstI C_TGCA'G
Cuts at: 830 2334 2582 3573 4244 830
Size: 1504 248 991 671 3478
Fragments arranged by size:
3478 1504 991 671 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6892
PvuII CAG'CTG
Cuts at: 2557 3051 2557
Size: 494 6398
SacI G_AGCT'C
Cuts at: 2249 2249
Size: 6892
SacII CC_GC'GG
Cuts at: 3792 3792
Size: 6892
SalI G'TCGA_C
Cuts at: 1936 4234 1936
Size: 2298 4594
SbfI CC_TGCA'GG
Cuts at: 4244 4244
Size: 6892
ScaI AGT'ACT
Cuts at: 5140 6852 5140
Size: 1712 5180
SfcI C'TryA_G
Cuts at: 826 1045 2114 2330 2410 2578 3569 4240 5375
Size: 219 1069 216 80 168 991 671 1135
Cuts at: 5375 6056 6247 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1069 991 681 671 219 216
191 168 80
SfoI GGC'GCC
Cuts at: 2957 2957
Size: 6892
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6892
SmaI CCC'GGG
Cuts at: 1872 4219 1872
Size: 2347 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2388 2648 3898 4016 4996 5897
Size: 197 926 445 260 1250 118 980 901
Cuts at: 5897 6159 820
Size: 262 1553
Fragments arranged by size:
1553 1250 980 926 901 445 262 260
197 118
SnaBI TAC'GTA
Cuts at: 493 4683 493
Size: 4190 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6892
SphI G_CATG'C
Cuts at: 1132 4250 1132
Size: 3118 3774
SspI AAT'ATT
Cuts at: 5 52 4816 5
Size: 47 4764 2081
Fragments arranged by size:
4764 2081 47
StuI AGG'CCT
Cuts at: 1517 2275 2399 1517
Size: 758 124 6010
Fragments arranged by size:
6010 758 124
StyI C'CwwG_G
Cuts at: 513 1065 2004 3024 3184 3270 3406 3633 4044
Size: 552 939 1020 160 86 136 227 411
Cuts at: 4044 4134 4331 513
Size: 90 197 3074
Fragments arranged by size:
3074 1020 939 552 411 227 197 160
136 90 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3032 4919 4936 5089 5274 5719 325
Size: 1255 1452 1887 17 153 185 445 1498
Fragments arranged by size:
1887 1498 1452 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5138 6850 96
Size: 274 80 33 51 1443 3161 1712 138
Fragments arranged by size:
3161 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3663 3695 3861 3893 3964 3996 6852 6884 3663
Size: 32 166 32 71 32 2856 32 3671
Fragments arranged by size:
3671 2856 166 71 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6892
XbaI T'CTAG_A
Cuts at: 4228 4228
Size: 6892
XhoI C'TCGA_G
Cuts at: 1943 2388 1943
Size: 445 6447
XmaI C'CCGG_G
Cuts at: 1870 4217 1870
Size: 2347 4545
XmnI GAAnn'nnTTC
Cuts at: 5021 5021
Size: 6892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2327 4700 276
Size: 53 83 186 936 793 2373 2468
Fragments arranged by size:
2468 2373 936 793 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I DraIII FseI FspAI NruI NsiI PacI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI