Restriction map for
pFN21AE4208
(Circular) (Six-base) MAPSORT of: pFN21AE4208.seq Check: 5955 from: 1 to: 6889
pFN21AE4208 6889 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4699 278
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4232 1937
Size: 2295 4594
Acc65I G'GTAC_C
Cuts at: 4210 4210
Size: 6889
AclI AA'CG_TT
Cuts at: 1563 5016 5389 1563
Size: 3453 373 3063
Fragments arranged by size:
3453 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2602 4952 6336 6604 1632
Size: 970 2350 1384 268 1917
Fragments arranged by size:
2350 1917 1384 970 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6692
AflIII A'CryG_T
Cuts at: 1203 2667 5788 6765 1203
Size: 1464 3121 977 1327
Fragments arranged by size:
3121 1464 1327 977
AgeI A'CCGG_T
Cuts at: 5720 5720
Size: 6889
AhdI GACnn_n'nnGTC
Cuts at: 5618 5618
Size: 6889
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2554 3275 1184
Size: 1370 721 4798
Fragments arranged by size:
4798 1370 721
AlwNI CAG_nnn'CTG
Cuts at: 4632 6204 4632
Size: 1572 5317
ApaLI G'TGCA_C
Cuts at: 4946 6102 4946
Size: 1156 5733
ApoI r'AATT_y
Cuts at: 1493 2030 2556 2996 3929 4036 4134 4198 4424
Size: 537 526 440 933 107 98 64 226
Cuts at: 4424 4576 6685 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 933 537 526 440 226 152
107 98 64
AseI AT'TA_AT
Cuts at: 160 1032 4118 5443 160
Size: 872 3086 1325 1606
Fragments arranged by size:
3086 1606 1325 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
AvaI C'yCGr_G
Cuts at: 1870 1943 2326 3816 4214 1870
Size: 73 383 1490 398 4545
Fragments arranged by size:
4545 1490 398 383 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3433 3466 3433
Size: 33 6856
BamHI G'GATC_C
Cuts at: 4219 4586 4219
Size: 367 6522
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3315 4210 5665 618
Size: 325 217 627 1528 895 1455 1842
Fragments arranged by size:
1842 1528 1455 895 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2097 4208 1391
Size: 706 2111 4072
Fragments arranged by size:
4072 2111 706
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3192 928
Size: 971 1293 4625
Fragments arranged by size:
4625 1293 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5080 5114 1903
Size: 34 3143 34 3678
Fragments arranged by size:
3678 3143 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4113 4781 5991 4113
Size: 668 1210 5011
Fragments arranged by size:
5011 1210 668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6889
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5500 136
Size: 107 122 71 631 605 3828 1525
Fragments arranged by size:
3828 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6884 6884
Size: 6889
BlpI GC'TnA_GC
Cuts at: 4306 4306
Size: 6889
Bme1580I G_kGCm'C
Cuts at: 1790 2642 4950 6106 1790
Size: 852 2308 1156 2573
Fragments arranged by size:
2573 2308 1156 852
BmgBI CAC'GTC
Cuts at: 1318 3259 1318
Size: 1941 4948
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4620 5578 442
Size: 416 53 83 399 24 3203 958 1753
Fragments arranged by size:
3203 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3513 3519 5549 1239
Size: 2274 6 2030 2579
Fragments arranged by size:
2579 2274 2030 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2481 3045 5014 5879 6177 2481
Size: 564 1969 865 298 3193
Fragments arranged by size:
3193 1969 865 564 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3600 5552 882
Size: 617 2101 1952 2219
Fragments arranged by size:
2219 2101 1952 617
BsaAI yAC'GTr
Cuts at: 493 1204 4680 493
Size: 711 3476 2702
Fragments arranged by size:
3476 2702 711
BsaBI GATnn'nnATC
Cuts at: 2370 4050 6883 2370
Size: 1680 2833 2376
Fragments arranged by size:
2833 2376 1680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4696 5078 5713 275
Size: 53 83 186 936 3163 382 635 1451
Fragments arranged by size:
3163 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5322 5720 5994 6141 6552 5322
Size: 398 274 147 411 5659
Fragments arranged by size:
5659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2241 2271 1178
Size: 30 165 30 838 30 5796
Fragments arranged by size:
5796 838 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2962 3516 1186
Size: 1776 554 4559
Fragments arranged by size:
4559 1776 554
BseYI C'CCAG_C
Cuts at: 2104 6092 6588 2104
Size: 3988 496 2405
Fragments arranged by size:
3988 2405 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3119 1163
Size: 573 1383 4933
Fragments arranged by size:
4933 1383 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4339 5100 6128 1540
Size: 275 186 2338 761 1028 2301
Fragments arranged by size:
2338 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 4950 5035 6106 2097
Size: 2853 85 1071 2880
Fragments arranged by size:
2880 2853 1071 85
BsmI GAATG_Cn'
Cuts at: 2552 4400 4493 2552
Size: 1848 93 4948
Fragments arranged by size:
4948 1848 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2642 4208 4950 5035 6106 1391
Size: 399 307 545 1566 742 85 1071 2174
Fragments arranged by size:
2174 1566 1071 742 545 399 307 85
BspEI T'CCGG_A
Cuts at: 6552 6552
Size: 6889
BspHI T'CATG_A
Cuts at: 4778 6508 4778
Size: 1730 5159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3172 4244 844
Size: 2328 1072 3489
Fragments arranged by size:
3489 2328 1072
BsrBI CCG'CTC
Cuts at: 1394 1823 4776 1394
Size: 429 2953 3507
Fragments arranged by size:
3507 2953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2548 5384 5558 65
Size: 1183 1300 2836 174 1396
Fragments arranged by size:
2836 1396 1300 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5533 5720 1700
Size: 110 3723 187 2869
Fragments arranged by size:
3723 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6889
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6889
BssSI C'ACGA_G
Cuts at: 4949 5961 4949
Size: 1012 5877
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2547 794
Size: 1753 5136
BstBI TT'CG_AA
Cuts at: 2404 5693 2404
Size: 3289 3600
BstEII G'GTnAC_C
Cuts at: 1184 2068 1184
Size: 884 6005
BstYI r'GATC_y
Cuts at: 1972 2371 4219 4586 4971 4988 6440 6526 6538
Size: 399 1848 367 385 17 1452 86 12
Cuts at: 6538 6884 1972
Size: 346 1977
Fragments arranged by size:
1977 1848 1452 399 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6150
Fragments arranged by size:
6150 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 4132 4405 5199 5219 2761
Size: 1371 273 794 20 4431
Fragments arranged by size:
4431 1371 794 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6854
DraI TTT'AAA
Cuts at: 4193 4193
Size: 6889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3085 5896 809
Size: 507 1769 2811 1802
Fragments arranged by size:
2811 1802 1769 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4336 5225 6854
Size: 54 1425 50 275 18 2506 889 1629
Cuts at: 6854 8
Size: 43
Fragments arranged by size:
2506 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4336 1537
Size: 275 2524 4090
Fragments arranged by size:
4090 2524 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 2931 3009 3146 3596 4819 1353
Size: 1469 109 78 137 450 1223 3423
Fragments arranged by size:
3423 1469 1223 450 137 109 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5476 5850 5996 387
Size: 1064 138 3887 374 146 1280
Fragments arranged by size:
3887 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2095 2095
Size: 6889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2602 3513 3519 4952 5549 6336 6604
Size: 393 970 911 6 1433 597 787 268
Cuts at: 6604 1239
Size: 1524
Fragments arranged by size:
1524 1433 970 911 787 597 393 268
6
EcoRI G'AATT_C
Cuts at: 4134 4198 4576 4134
Size: 64 378 6447
Fragments arranged by size:
6447 378 64
EcoRV GAT'ATC
Cuts at: 2438 2438
Size: 6889
FspI TGC'GCA
Cuts at: 5395 5395
Size: 6889
HaeII r_GCGC'y
Cuts at: 1678 6036 1678
Size: 4358 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2241 2273 2930
Size: 32 135 32 632 32 5 32 657
Cuts at: 2930 2962 3371 3403 5530 5562 5604 5636 1373
Size: 32 409 32 2127 32 42 32 2626
Fragments arranged by size:
2626 2127 657 632 409 135 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4233 4479 669
Size: 988 281 2295 246 3079
Fragments arranged by size:
3079 2295 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2780 3679 748
Size: 2032 899 3958
Fragments arranged by size:
3958 2032 899
HpaI GTT'AAC
Cuts at: 4479 4479
Size: 6889
KpnI G_GTAC'C
Cuts at: 4214 4214
Size: 6889
MfeI C'AATT_G
Cuts at: 2964 4488 2964
Size: 1524 5365
MluI A'CGCG_T
Cuts at: 6765 6765
Size: 6889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2630 6003 6187 1305
Size: 684 641 3373 184 2007
Fragments arranged by size:
3373 2007 684 641 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5067
Fragments arranged by size:
5067 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2169 2595 3257 3662 3861 4847 5206
Size: 648 1003 426 662 405 199 986 359
Cuts at: 5206 5365 518
Size: 159 2042
Fragments arranged by size:
2042 1003 986 662 648 426 405 359
199 159
MspA1I CmG'CkG
Cuts at: 1606 4305 4982 6130 6375 1606
Size: 2699 677 1148 245 2120
Fragments arranged by size:
2699 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6337
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6889
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6889
NotI GC'GGCC_GC
Cuts at: 4336 4336
Size: 6889
NspI r_CATG'y
Cuts at: 1132 1229 2112 2671 3925 4247 5792 1132
Size: 97 883 559 1254 322 1545 2229
Fragments arranged by size:
2229 1545 1254 883 559 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6889
PciI A'CATG_T
Cuts at: 2667 5788 2667
Size: 3121 3768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6889
PmeI GTTT'AAAC
Cuts at: 4193 4193
Size: 6889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4958 4990 6471 6503 4958
Size: 32 1481 32 5344
Fragments arranged by size:
5344 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6889
PsiI TTA'TAA
Cuts at: 3294 4459 3294
Size: 1165 5724
PstI C_TGCA'G
Cuts at: 830 3169 3652 4241 830
Size: 2339 483 589 3478
Fragments arranged by size:
3478 2339 589 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6889
SacI G_AGCT'C
Cuts at: 2097 2097
Size: 6889
SalI G'TCGA_C
Cuts at: 1936 4231 1936
Size: 2295 4594
SapI GCTCTTCn'nnn_
Cuts at: 3146 3146
Size: 6889
SbfI CC_TGCA'GG
Cuts at: 4241 4241
Size: 6889
ScaI AGT'ACT
Cuts at: 5137 6849 5137
Size: 1712 5177
SexAI A'CCwGG_T
Cuts at: 3759 3759
Size: 6889
SfcI C'TryA_G
Cuts at: 826 1045 2524 3165 3490 3648 3915 4237 5372
Size: 219 1479 641 325 158 267 322 1135
Cuts at: 5372 6053 6244 826
Size: 681 191 1471
Fragments arranged by size:
1479 1471 1135 681 641 325 322 267
219 191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6889
SmaI CCC'GGG
Cuts at: 1872 3818 4216 1872
Size: 1946 398 4545
Fragments arranged by size:
4545 1946 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2496 3060 4993 5894 6156 820
Size: 197 926 553 564 1933 901 262 1553
Fragments arranged by size:
1933 1553 926 901 564 553 262 197
SnaBI TAC'GTA
Cuts at: 493 4680 493
Size: 4187 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6889
SphI G_CATG'C
Cuts at: 1132 2112 3925 4247 1132
Size: 980 1813 322 3774
Fragments arranged by size:
3774 1813 980 322
SspI AAT'ATT
Cuts at: 5 52 4813 5
Size: 47 4761 2081
Fragments arranged by size:
4761 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6889
StyI C'CwwG_G
Cuts at: 513 1065 2567 4328 513
Size: 552 1502 1761 3074
Fragments arranged by size:
3074 1761 1502 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2145 2301 2705 4916 4933 5086 5271
Size: 1255 565 156 404 2211 17 153 185
Cuts at: 5271 5716 325
Size: 445 1498
Fragments arranged by size:
2211 1498 1255 565 445 404 185 156
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 5135 6847
Size: 274 80 33 51 1443 693 2465 1712
Cuts at: 6847 96
Size: 138
Fragments arranged by size:
2465 1712 1443 693 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6849 6881 6849
Size: 32 6857
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6889
XbaI T'CTAG_A
Cuts at: 3031 4225 3031
Size: 1194 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6889
XmaI C'CCGG_G
Cuts at: 1870 3816 4214 1870
Size: 1946 398 4545
Fragments arranged by size:
4545 1946 398
XmnI GAAnn'nnTTC
Cuts at: 3513 5018 3513
Size: 1505 5384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4697 276
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI
SfoI SrfI SwaI XcmI