Restriction map for
pFN21AE4205
(Circular) (Six-base) MAPSORT of: pFN21AE4205.seq Check: 1717 from: 1 to: 6874
pFN21AE4205 6874 bp
With 182 enzymes: SgfI *
March 28, 2012 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2126 2771 2126
Size: 645 6229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4684 278
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4217 1937
Size: 2280 4594
Acc65I G'GTAC_C
Cuts at: 4195 4195
Size: 6874
AclI AA'CG_TT
Cuts at: 1563 5001 5374 1563
Size: 3438 373 3063
Fragments arranged by size:
3438 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3001 3149 3326 3613 4160 4937 6321 6589
Size: 1369 148 177 287 547 777 1384 268
Cuts at: 6589 1632
Size: 1917
Fragments arranged by size:
1917 1384 1369 777 547 287 268 177
148
AfeI AGC'GCT
Cuts at: 2654 2753 2654
Size: 99 6775
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6677
AflIII A'CryG_T
Cuts at: 1203 3875 4029 5773 6750 1203
Size: 2672 154 1744 977 1327
Fragments arranged by size:
2672 1744 1327 977 154
AgeI A'CCGG_T
Cuts at: 5705 5705
Size: 6874
AhdI GACnn_n'nnGTC
Cuts at: 3748 5603 3748
Size: 1855 5019
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6874
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2474 2627 1184
Size: 1290 153 5431
Fragments arranged by size:
5431 1290 153
AlwNI CAG_nnn'CTG
Cuts at: 3841 4617 6189 3841
Size: 776 1572 4526
Fragments arranged by size:
4526 1572 776
ApaI G_GGCC'C
Cuts at: 2709 3486 2709
Size: 777 6097
ApaLI G'TGCA_C
Cuts at: 3533 4931 6087 3533
Size: 1398 1156 4320
Fragments arranged by size:
4320 1398 1156
ApoI r'AATT_y
Cuts at: 1493 3074 3911 3969 4183 4409 4561 6670 1493
Size: 1581 837 58 214 226 152 2109 1697
Fragments arranged by size:
2109 1697 1581 837 226 214 152 58
AseI AT'TA_AT
Cuts at: 160 1032 5428 160
Size: 872 4396 1606
Fragments arranged by size:
4396 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 4199 1870
Size: 73 678 1578 4545
Fragments arranged by size:
4545 1578 678 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2370 2403 2370
Size: 33 6841
BamHI G'GATC_C
Cuts at: 4204 4571 4204
Size: 367 6507
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4195 5650 618
Size: 325 217 627 2408 1455 1842
Fragments arranged by size:
2408 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2186 2709 3471 3486 3682 3960 4193 1391
Size: 795 523 762 15 196 278 233 4072
Fragments arranged by size:
4072 795 762 523 278 233 196 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3815 3894 928
Size: 971 1916 79 3908
Fragments arranged by size:
3908 1916 971 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5065 5099 1903
Size: 34 3128 34 3678
Fragments arranged by size:
3678 3128 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4766 5976 4766
Size: 1210 5664
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6874
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5485 136
Size: 107 122 71 631 605 3813 1525
Fragments arranged by size:
3813 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2868 6869 2868
Size: 4001 2873
BlpI GC'TnA_GC
Cuts at: 4291 4291
Size: 6874
Bme1580I G_kGCm'C
Cuts at: 1790 2709 3432 3486 3537 4935 6091 1790
Size: 919 723 54 51 1398 1156 2573
Fragments arranged by size:
2573 1398 1156 919 723 54 51
BmgBI CAC'GTC
Cuts at: 1318 2830 3892 1318
Size: 1512 1062 4300
Fragments arranged by size:
4300 1512 1062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 2564 3676
Size: 416 53 83 399 24 959 188 1112
Cuts at: 3676 4605 5563 442
Size: 929 958 1753
Fragments arranged by size:
1753 1112 959 958 929 416 399 188
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3683 3748 3756 4016 4145 5534 1239
Size: 2444 65 8 260 129 1389 2579
Fragments arranged by size:
2579 2444 1389 260 129 65 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2777 3131 4999 5864 6162 2663
Size: 114 354 1868 865 298 3375
Fragments arranged by size:
3375 1868 865 354 298 114
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5537 882
Size: 617 4038 2219
Fragments arranged by size:
4038 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3427 4030 4665 493
Size: 711 2223 603 635 2702
Fragments arranged by size:
2702 2223 711 635 603
BsaBI GATnn'nnATC
Cuts at: 2873 6868 2873
Size: 3995 2879
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2641 2988 4681 5063
Size: 53 83 186 936 1108 347 1693 382
Cuts at: 5063 5698 275
Size: 635 1451
Fragments arranged by size:
1693 1451 1108 936 635 382 347 186
83 53
BsaWI w'CCGG_w
Cuts at: 5307 5705 5979 6126 6537 5307
Size: 398 274 147 411 5644
Fragments arranged by size:
5644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2684 2714 2978 3008 3919
Size: 30 165 30 1281 30 264 30 911
Cuts at: 3919 3949 1178
Size: 30 4103
Fragments arranged by size:
4103 1281 911 264 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2335 2689 3490 3604 3924 1186
Size: 1149 354 801 114 320 4136
Fragments arranged by size:
4136 1149 801 354 320 114
BseYI C'CCAG_C
Cuts at: 6077 6573 6077
Size: 496 6378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2098 2152 2227 2450 2470 2618 2917
Size: 573 362 54 75 223 20 148 299
Cuts at: 2917 3395 3986 1163
Size: 478 591 4051
Fragments arranged by size:
4051 591 573 478 362 299 223 148
75 54 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2581 4324 5085 6113 1540
Size: 275 186 580 1743 761 1028 2301
Fragments arranged by size:
2301 1743 1028 761 580 275 186
BsiHKAI G_wGCw'C
Cuts at: 2670 3471 3537 3960 4935 5020 6091 2670
Size: 801 66 423 975 85 1071 3453
Fragments arranged by size:
3453 1071 975 801 423 85 66
BsmI GAATG_Cn'
Cuts at: 3159 3326 4385 4478 3159
Size: 167 1059 93 5555
Fragments arranged by size:
5555 1059 167 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2648 2648
Size: 6874
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2670 2709 3432 3471 3486 3537
Size: 399 396 484 39 723 39 15 51
Cuts at: 3537 3682 3960 4193 4935 5020 6091 1391
Size: 145 278 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 484 399 396 278
233 145 85 51 39 39 15
BspEI T'CCGG_A
Cuts at: 6537 6537
Size: 6874
BspHI T'CATG_A
Cuts at: 4763 6493 4763
Size: 1730 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 2640 2771 4229 844
Size: 1282 514 131 1458 3489
Fragments arranged by size:
3489 1458 1282 514 131
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 2146 2583 2697 4761 1394
Size: 429 284 39 437 114 2064 3507
Fragments arranged by size:
3507 2064 437 429 284 114 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 2920 4076 5369 5543 65
Size: 1183 1672 1156 1293 174 1396
Fragments arranged by size:
1672 1396 1293 1183 1156 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2454 5518 5705 1700
Size: 110 644 3064 187 2869
Fragments arranged by size:
3064 2869 644 187 110
BsrGI T'GTAC_A
Cuts at: 96 2039 3754 96
Size: 1943 1715 3216
Fragments arranged by size:
3216 1943 1715
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6874
BssSI C'ACGA_G
Cuts at: 4934 5946 4934
Size: 1012 5862
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2593 2763 2787 2940 794
Size: 1799 170 24 153 4728
Fragments arranged by size:
4728 1799 170 153 24
BstBI TT'CG_AA
Cuts at: 5678 5678
Size: 6874
BstEII G'GTnAC_C
Cuts at: 1184 2539 1184
Size: 1355 5519
BstXI CCAn_nnnn'nTGG
Cuts at: 3349 3349
Size: 6874
BstYI r'GATC_y
Cuts at: 1972 2868 3345 4204 4571 4956 4973 6425 6511
Size: 896 477 859 367 385 17 1452 86
Cuts at: 6511 6523 6869 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 896 859 477 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2327 3502 2327
Size: 1175 5699
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5383
Fragments arranged by size:
5383 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2725 3010 487
Size: 683 56 1499 285 4351
Fragments arranged by size:
4351 1499 683 285 56
BtsI GCAGTG_nn'
Cuts at: 2164 2587 4390 5184 5204 2164
Size: 423 1803 794 20 3834
Fragments arranged by size:
3834 1803 794 423 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6874
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2420 2455 529
Size: 35 1856 35 4948
Fragments arranged by size:
4948 1856 35 35
DraI TTT'AAA
Cuts at: 3100 4036 4178 3100
Size: 936 142 5796
Fragments arranged by size:
5796 936 142
DraIII CAC_nnn'GTG
Cuts at: 2425 2425
Size: 6874
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5881 809
Size: 507 4565 1802
Fragments arranged by size:
4565 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2814 2962 3316
Size: 54 1425 50 275 18 984 148 354
Cuts at: 3316 4321 5210 6839 8
Size: 1005 889 1629 43
Fragments arranged by size:
1629 1425 1005 984 889 354 275 148
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4321 1537
Size: 275 2509 4090
Fragments arranged by size:
4090 2509 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3020 3476 4804 1353
Size: 1667 456 1328 3423
Fragments arranged by size:
3423 1667 1328 456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5461 5835 5981 387
Size: 1064 138 3872 374 146 1280
Fragments arranged by size:
3872 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3469 3958 3469
Size: 489 6385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3001 3149 3326 3613 3683 3748 3756
Size: 393 1369 148 177 287 70 65 8
Cuts at: 3756 4016 4145 4160 4937 5534 6321 6589 1239
Size: 260 129 15 777 597 787 268 1524
Fragments arranged by size:
1524 1369 787 777 597 393 287 268
260 177 148 129 70 65 15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2717 2717
Size: 6874
EcoO109I rG'GnC_Cy
Cuts at: 3482 4132 3482
Size: 650 6224
EcoRI G'AATT_C
Cuts at: 3074 3911 4183 4561 3074
Size: 837 272 378 5387
Fragments arranged by size:
5387 837 378 272
EcoRV GAT'ATC
Cuts at: 4081 4081
Size: 6874
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2704 2110
Size: 594 6280
FspI TGC'GCA
Cuts at: 2363 3155 5380 2363
Size: 792 2225 3857
Fragments arranged by size:
3857 2225 792
HaeII r_GCGC'y
Cuts at: 1678 2656 2689 2755 2850 3024 6021 1678
Size: 978 33 66 95 174 2997 2531
Fragments arranged by size:
2997 2531 978 174 95 66 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2041 2073 2684 2716 2978
Size: 32 135 32 469 32 611 32 262
Cuts at: 2978 3010 3187 3219 3262 3294 3734 3766 3881
Size: 32 177 32 43 32 440 32 115
Cuts at: 3881 3913 5515 5547 5589 5621 1373
Size: 32 1602 32 42 32 2626
Fragments arranged by size:
2626 1602 611 469 440 262 177 135
115 43 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4218 4464 669
Size: 988 281 2280 246 3079
Fragments arranged by size:
3079 2280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6874
HpaI GTT'AAC
Cuts at: 4464 4464
Size: 6874
KpnI G_GTAC'C
Cuts at: 4199 4199
Size: 6874
MfeI C'AATT_G
Cuts at: 2437 3822 4473 2437
Size: 1385 651 4838
Fragments arranged by size:
4838 1385 651
MluI A'CGCG_T
Cuts at: 6750 6750
Size: 6874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5988 6172 1305
Size: 4683 184 2007
Fragments arranged by size:
4683 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2816 2964 3318 10
Size: 54 1425 343 984 148 354 3566
Fragments arranged by size:
3566 1425 984 354 343 148 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2808 3260 4832 5191 5350 518
Size: 648 1642 452 1572 359 159 2042
Fragments arranged by size:
2042 1642 1572 648 452 359 159
MspA1I CmG'CkG
Cuts at: 1606 2286 2521 2617 4290 4967 6115 6360 1606
Size: 680 235 96 1673 677 1148 245 2120
Fragments arranged by size:
2120 1673 1148 680 677 245 235 96
NaeI GCC'GGC
Cuts at: 2456 2456
Size: 6874
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5383
Fragments arranged by size:
5383 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6874
NgoMIV G'CCGG_C
Cuts at: 2454 2454
Size: 6874
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6874
NotI GC'GGCC_GC
Cuts at: 4321 4321
Size: 6874
NspI r_CATG'y
Cuts at: 1132 1229 2596 2662 3879 4232 5777 1132
Size: 97 1367 66 1217 353 1545 2229
Fragments arranged by size:
2229 1545 1367 1217 353 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6874
PciI A'CATG_T
Cuts at: 3875 5773 3875
Size: 1898 4976
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3841 1108
Size: 2733 4141
PmeI GTTT'AAAC
Cuts at: 4178 4178
Size: 6874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4943 4975 6456 6488 4943
Size: 32 1481 32 5329
Fragments arranged by size:
5329 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6874
PsiI TTA'TAA
Cuts at: 4444 4444
Size: 6874
PspOMI G'GGCC_C
Cuts at: 2705 3482 2705
Size: 777 6097
PstI C_TGCA'G
Cuts at: 830 2526 3336 4226 830
Size: 1696 810 890 3478
Fragments arranged by size:
3478 1696 890 810
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6874
PvuII CAG'CTG
Cuts at: 2521 2617 2521
Size: 96 6778
SacI G_AGCT'C
Cuts at: 3471 3960 3471
Size: 489 6385
SalI G'TCGA_C
Cuts at: 1936 4216 1936
Size: 2280 4594
SapI GCTCTTCn'nnn_
Cuts at: 3476 3476
Size: 6874
SbfI CC_TGCA'GG
Cuts at: 4226 4226
Size: 6874
ScaI AGT'ACT
Cuts at: 2906 5122 6834 2906
Size: 2216 1712 2946
Fragments arranged by size:
2946 2216 1712
SexAI A'CCwGG_T
Cuts at: 2421 2421
Size: 6874
SfcI C'TryA_G
Cuts at: 826 1045 2522 2599 3332 3385 4222 5357 6038
Size: 219 1477 77 733 53 837 1135 681
Cuts at: 6038 6229 826
Size: 191 1471
Fragments arranged by size:
1477 1471 1135 837 733 681 219 191
77 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6874
SmaI CCC'GGG
Cuts at: 1872 4201 1872
Size: 2329 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 2792 3146 4978 5879 6141
Size: 197 926 735 114 354 1832 901 262
Cuts at: 6141 820
Size: 1553
Fragments arranged by size:
1832 1553 926 901 735 354 262 197
114
SnaBI TAC'GTA
Cuts at: 493 4665 493
Size: 4172 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6874
SphI G_CATG'C
Cuts at: 1132 2596 4232 1132
Size: 1464 1636 3774
Fragments arranged by size:
3774 1636 1464
SspI AAT'ATT
Cuts at: 5 52 4798 5
Size: 47 4746 2081
Fragments arranged by size:
4746 2081 47
StuI AGG'CCT
Cuts at: 1517 2798 3391 3668 1517
Size: 1281 593 277 4723
Fragments arranged by size:
4723 1281 593 277
StyI C'CwwG_G
Cuts at: 513 1065 2004 3294 3432 3651 3861 4313 513
Size: 552 939 1290 138 219 210 452 3074
Fragments arranged by size:
3074 1290 939 552 452 219 210 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2898 3590 4901 4918 5071 5256 5701
Size: 1255 1318 692 1311 17 153 185 445
Cuts at: 5701 325
Size: 1498
Fragments arranged by size:
1498 1318 1311 1255 692 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2039 2904 3754
Size: 274 80 33 51 1443 62 865 850
Cuts at: 3754 5120 6832 96
Size: 1366 1712 138
Fragments arranged by size:
1712 1443 1366 865 850 274 138 80
62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2870 2902 6834 6866 2870
Size: 32 3932 32 2878
Fragments arranged by size:
3932 2878 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2828 1316
Size: 1512 5362
XbaI T'CTAG_A
Cuts at: 3205 4210 3205
Size: 1005 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2040 2419 3349 3658 2040
Size: 379 930 309 5256
Fragments arranged by size:
5256 930 379 309
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6874
XmaI C'CCGG_G
Cuts at: 1870 4199 1870
Size: 2329 4545
XmnI GAAnn'nnTTC
Cuts at: 2552 4169 5003 2552
Size: 1617 834 4423
Fragments arranged by size:
4423 1617 834
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4682 276
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI