Restriction map for
pFN21AE4196
(Circular) (Six-base) MAPSORT of: pFN21AE4196.seq Check: 8200 from: 1 to: 6823
pFN21AE4196 6823 bp
With 182 enzymes: SgfI *
March 28, 2012 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2856 2856
Size: 6823
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2708 4166 1937
Size: 771 1458 4594
Fragments arranged by size:
4594 1458 771
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2750 4886 6270 6538 1632
Size: 1118 2136 1384 268 1917
Fragments arranged by size:
2136 1917 1384 1118 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6626
AflIII A'CryG_T
Cuts at: 1203 3090 3978 5722 6699 1203
Size: 1887 888 1744 977 1327
Fragments arranged by size:
1887 1744 1327 977 888
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 5552 5552
Size: 6823
AleI CACnn'nnGTG
Cuts at: 1166 3077 1166
Size: 1911 4912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2564 3095 3176 1184
Size: 1380 531 81 4831
Fragments arranged by size:
4831 1380 531 81
AlwNI CAG_nnn'CTG
Cuts at: 2171 2206 4566 6138 2171
Size: 35 2360 1572 2856
Fragments arranged by size:
2856 2360 1572 35
ApaI G_GGCC'C
Cuts at: 3124 3574 3124
Size: 450 6373
ApaLI G'TGCA_C
Cuts at: 4880 6036 4880
Size: 1156 5667
ApoI r'AATT_y
Cuts at: 1493 2993 4132 4358 4510 6619 1493
Size: 1500 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1500 1139 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5377 160
Size: 872 4345 1606
Fragments arranged by size:
4345 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 3959 3968 4148 1870
Size: 73 2016 9 180 4545
Fragments arranged by size:
4545 2016 180 73 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3220 3253 3220
Size: 33 6790
BamHI G'GATC_C
Cuts at: 3774 4153 4520 3774
Size: 379 367 6077
Fragments arranged by size:
6077 379 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2910 3955 4044 4144
Size: 325 217 627 334 789 1045 89 100
Cuts at: 4144 5599 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1045 789 627 334 325 217
100 89
BanII G_rGCy'C
Cuts at: 1391 2181 3048 3124 3384 3442 3574 4074 4142
Size: 790 867 76 260 58 132 500 68
Cuts at: 4142 1391
Size: 4072
Fragments arranged by size:
4072 867 790 500 260 132 76 68
58
BbeI G_GCGC'C
Cuts at: 2125 2125
Size: 6823
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2738 928
Size: 971 839 5013
Fragments arranged by size:
5013 971 839
BbvCI CC'TCA_GC
Cuts at: 3309 3309
Size: 6823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4091 4125 5014 5048 1903
Size: 34 2154 34 889 34 3678
Fragments arranged by size:
3678 2154 889 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2363 2629 4715 5925 2363
Size: 266 2086 1210 3261
Fragments arranged by size:
3261 2086 1210 266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6823
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2130 5434 136
Size: 107 122 71 631 605 458 3304 1525
Fragments arranged by size:
3304 1525 631 605 458 122 107 71
BglII A'GATC_T
Cuts at: 6818 6818
Size: 6823
BlpI GC'TnA_GC
Cuts at: 4240 4240
Size: 6823
Bme1580I G_kGCm'C
Cuts at: 1790 3124 3574 4049 4884 6040 1790
Size: 1334 450 475 835 1156 2573
Fragments arranged by size:
2573 1334 1156 835 475 450
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2921 3445 4554
Size: 416 53 83 399 24 1504 524 1109
Cuts at: 4554 5512 442
Size: 958 1753
Fragments arranged by size:
1753 1504 1109 958 524 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3545 1056
Size: 2489 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2677 3134 3407 3533 3679 5483 1239
Size: 1438 457 273 126 146 1804 2579
Fragments arranged by size:
2579 1804 1438 457 273 146 126
Bpu10I CC'TnA_GC
Cuts at: 3309 3309
Size: 6823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4948 5813 6111 4948
Size: 865 298 5660
Fragments arranged by size:
5660 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 5486 882
Size: 617 699 3288 2219
Fragments arranged by size:
3288 2219 699 617
BsaAI yAC'GTr
Cuts at: 493 1204 3091 3139 3979 4614 493
Size: 711 1887 48 840 635 2702
Fragments arranged by size:
2702 1887 840 711 635 48
BsaBI GATnn'nnATC
Cuts at: 6817 6817
Size: 6823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2122 3965 4630 5012
Size: 53 83 186 936 589 1843 665 382
Cuts at: 5012 5647 275
Size: 635 1451
Fragments arranged by size:
1843 1451 936 665 635 589 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3726 5256 5654 5928 6075 6486 3726
Size: 1530 398 274 147 411 4063
Fragments arranged by size:
4063 1530 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2894 2924 1178
Size: 30 165 30 1491 30 5077
Fragments arranged by size:
5077 1491 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2828 3326 3574 1186
Size: 1642 498 248 4435
Fragments arranged by size:
4435 1642 498 248
BseYI C'CCAG_C
Cuts at: 2158 2171 2283 2338 2443 2686 3032 3040 3057
Size: 13 112 55 105 243 346 8 17
Cuts at: 3057 3274 3627 4023 6026 6522 2158
Size: 217 353 396 2003 496 2459
Fragments arranged by size:
2459 2003 496 396 353 346 243 217
112 105 55 17 13 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2434 3119 3136 3881 4039 1163
Size: 573 354 344 685 17 745 158 3947
Fragments arranged by size:
3947 745 685 573 354 344 158 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4273 5034 6062 1540
Size: 275 186 2272 761 1028 2301
Fragments arranged by size:
2301 2272 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3074 3384 4074 4884 4969 6040 3074
Size: 310 690 810 85 1071 3857
Fragments arranged by size:
3857 1071 810 690 310 85
BsmI GAATG_Cn'
Cuts at: 4334 4427 4334
Size: 93 6730
BsmBI CGTCTCn'nnnn_
Cuts at: 2609 3956 2609
Size: 1347 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 3048 3074 3124 3384 3442 3574
Size: 399 391 867 26 50 260 58 132
Cuts at: 3574 4049 4074 4142 4884 4969 6040 1391
Size: 475 25 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 867 742 475 399 391 260
132 85 68 58 50 26 25
BspEI T'CCGG_A
Cuts at: 3726 6486 3726
Size: 2760 4063
BspHI T'CATG_A
Cuts at: 4712 6442 4712
Size: 1730 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2538 2856 4178 844
Size: 1694 318 1322 3489
Fragments arranged by size:
3489 1694 1322 318
BsrBI CCG'CTC
Cuts at: 1394 1823 3913 4710 1394
Size: 429 2090 797 3507
Fragments arranged by size:
3507 2090 797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2886 3373 5318 5492 65
Size: 1183 1638 487 1945 174 1396
Fragments arranged by size:
1945 1638 1396 1183 487 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3933 5467 5654 1700
Size: 110 2123 1534 187 2869
Fragments arranged by size:
2869 2123 1534 187 110
BsrGI T'GTAC_A
Cuts at: 96 2253 96
Size: 2157 4666
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 4883 5895 4883
Size: 1012 5811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3142 794
Size: 2348 4475
BstBI TT'CG_AA
Cuts at: 3556 5627 3556
Size: 2071 4752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6823
BstXI CCAn_nnnn'nTGG
Cuts at: 2338 2392 2899 2338
Size: 54 507 6262
Fragments arranged by size:
6262 507 54
BstYI r'GATC_y
Cuts at: 1972 3774 4153 4520 4905 4922 6374 6460 6472
Size: 1802 379 367 385 17 1452 86 12
Cuts at: 6472 6818 1972
Size: 346 1977
Fragments arranged by size:
1977 1802 1452 385 379 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2631 3845 2631
Size: 1214 5609
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2277 2454 2640 3901 513
Size: 552 939 60 213 177 186 1261 3435
Fragments arranged by size:
3435 1261 939 552 213 186 177 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2424 3721 487
Size: 683 56 1198 1297 3589
Fragments arranged by size:
3589 1297 1198 683 56
BtsI GCAGTG_nn'
Cuts at: 2145 3392 4107 4339 5133 5153 2145
Size: 1247 715 232 794 20 3815
Fragments arranged by size:
3815 1247 794 715 232 20
ClaI AT'CG_AT
Cuts at: 1554 3255 1554
Size: 1701 5122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6788
DraI TTT'AAA
Cuts at: 4127 4127
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3970 5830 809
Size: 507 2654 1860 1802
Fragments arranged by size:
2654 1860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 4270 5159
Size: 54 1425 50 275 18 354 2086 889
Cuts at: 5159 6788 8
Size: 1629 43
Fragments arranged by size:
2086 1629 1425 889 354 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4270 1537
Size: 275 2458 4090
Fragments arranged by size:
4090 2458 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 3015 4062 4081 4753 1353
Size: 804 858 1047 19 672 3423
Fragments arranged by size:
3423 1047 858 804 672 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2566 3455 3902 4072 5410 5784
Size: 1064 138 977 889 447 170 1338 374
Cuts at: 5784 5930 387
Size: 146 1280
Fragments arranged by size:
1338 1280 1064 977 889 447 374 170
146 138
EcoICRI GAG'CTC
Cuts at: 3382 4072 3382
Size: 690 6133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2677 2750 3134 3407 3533 3679 4886
Size: 393 1045 73 384 273 126 146 1207
Cuts at: 4886 5483 6270 6538 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1207 1045 787 597 393 384 273
268 146 126 73
EcoNI CCTnn'n_nnAGG
Cuts at: 3704 3704
Size: 6823
EcoO109I rG'GnC_Cy
Cuts at: 2289 2527 2590 2830 3121 3570 3571 3651 2289
Size: 238 63 240 291 449 1 80 5461
Fragments arranged by size:
5461 449 291 240 238 80 63 1
EcoRI G'AATT_C
Cuts at: 4132 4510 4132
Size: 378 6445
EcoRV GAT'ATC
Cuts at: 3892 3892
Size: 6823
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2125 2172 3940 5970 1678
Size: 447 47 1768 2030 2531
Fragments arranged by size:
2531 2030 1768 447 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 2406 2438 5464
Size: 32 135 32 709 32 93 32 3026
Cuts at: 5464 5496 5538 5570 1373
Size: 32 42 32 2626
Fragments arranged by size:
3026 2626 709 135 93 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2709 4167 4413 669
Size: 988 281 771 1458 246 3079
Fragments arranged by size:
3079 1458 988 771 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KasI G'GCGC_C
Cuts at: 2121 2121
Size: 6823
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5937 6121 1305
Size: 4632 184 2007
Fragments arranged by size:
4632 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 10
Size: 54 1425 343 354 4647
Fragments arranged by size:
4647 1425 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2025 2390 2927 3077 4781 5140 5299
Size: 648 859 365 537 150 1704 359 159
Cuts at: 5299 518
Size: 2042
Fragments arranged by size:
2042 1704 859 648 537 365 359 159
150
MspA1I CmG'CkG
Cuts at: 1606 2307 2778 3883 3903 3924 4042 4239 4916
Size: 701 471 1105 20 21 118 197 677
Cuts at: 4916 6064 6309 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1105 701 677 471 245 197
118 21 20
NaeI GCC'GGC
Cuts at: 3935 3935
Size: 6823
NarI GG'CG_CC
Cuts at: 2122 2122
Size: 6823
NcoI C'CATG_G
Cuts at: 513 1065 2004 2277 2640 513
Size: 552 939 273 363 4696
Fragments arranged by size:
4696 939 552 363 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6823
NgoMIV G'CCGG_C
Cuts at: 3933 3933
Size: 6823
NheI G'CTAG_C
Cuts at: 1052 3541 1052
Size: 2489 4334
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 3176 3616 4036 4181 5726 1132
Size: 97 1947 440 420 145 1545 2229
Fragments arranged by size:
2229 1947 1545 440 420 145 97
PasI CC'CwG_GG
Cuts at: 1348 2662 3124 1348
Size: 1314 462 5047
Fragments arranged by size:
5047 1314 462
PciI A'CATG_T
Cuts at: 5722 5722
Size: 6823
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2283 3634 3883 1108
Size: 1175 1351 249 4048
Fragments arranged by size:
4048 1351 1175 249
PfoI T'CCnGG_A
Cuts at: 3384 3384
Size: 6823
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PmlI CAC'GTG
Cuts at: 3139 3139
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 4892 4924 6405 6437 3569
Size: 32 1291 32 1481 32 3955
Fragments arranged by size:
3955 1481 1291 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2289 2527 2590 2830 2289
Size: 238 63 240 6282
Fragments arranged by size:
6282 240 238 63
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PspOMI G'GGCC_C
Cuts at: 3120 3570 3120
Size: 450 6373
PstI C_TGCA'G
Cuts at: 830 2466 3486 3670 4175 830
Size: 1636 1020 184 505 3478
Fragments arranged by size:
3478 1636 1020 505 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
PvuII CAG'CTG
Cuts at: 2778 2778
Size: 6823
SacI G_AGCT'C
Cuts at: 3384 4074 3384
Size: 690 6133
SacII CC_GC'GG
Cuts at: 3904 3904
Size: 6823
SalI G'TCGA_C
Cuts at: 1936 2707 4165 1936
Size: 771 1458 4594
Fragments arranged by size:
4594 1458 771
SanDI GG'GwC_CC
Cuts at: 2830 2830
Size: 6823
SapI GCTCTTCn'nnn_
Cuts at: 2157 4062 2157
Size: 1905 4918
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 3488 5071 6783 3488
Size: 1583 1712 3528
Fragments arranged by size:
3528 1712 1583
SfcI C'TryA_G
Cuts at: 826 1045 2104 2462 3482 3666 4171 5306 5987
Size: 219 1059 358 1020 184 505 1135 681
Cuts at: 5987 6178 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1059 1020 681 505 358 219
191 184
SfoI GGC'GCC
Cuts at: 2123 2123
Size: 6823
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 4150 1872
Size: 2278 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4927 5828 6090 820
Size: 197 926 2984 901 262 1553
Fragments arranged by size:
2984 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 2323 152
Size: 2171 4652
SphI G_CATG'C
Cuts at: 1132 3176 4181 1132
Size: 2044 1005 3774
Fragments arranged by size:
3774 2044 1005
SspI AAT'ATT
Cuts at: 5 52 3007 4747 5
Size: 47 2955 1740 2081
Fragments arranged by size:
2955 2081 1740 47
StuI AGG'CCT
Cuts at: 1517 3353 1517
Size: 1836 4987
StyI C'CwwG_G
Cuts at: 513 1065 2004 2277 2376 2640 2892 4262 513
Size: 552 939 273 99 264 252 1370 3074
Fragments arranged by size:
3074 1370 939 552 273 264 252 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2858 4850 4867 5020 5205 5650 325
Size: 1255 1278 1992 17 153 185 445 1498
Fragments arranged by size:
1992 1498 1278 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 3486 4008
Size: 274 80 33 51 1443 276 1233 522
Cuts at: 4008 5069 6781 96
Size: 1061 1712 138
Fragments arranged by size:
1712 1443 1233 1061 522 276 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2894 2926 6783 6815 2894
Size: 32 3857 32 2902
Fragments arranged by size:
3857 2902 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6823
XbaI T'CTAG_A
Cuts at: 4159 4159
Size: 6823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2975 2975
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 4148 1870
Size: 2278 4545
XmnI GAAnn'nnTTC
Cuts at: 4952 4952
Size: 6823
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BstZ17I
DraIII FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SexAI SfiI SrfI SwaI