Restriction map for
pFN21AE4189
(Circular) (Six-base) MAPSORT of: pFN21AE4189.seq Check: 8303 from: 1 to: 6730
pFN21AE4189 6730 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4540 278
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2364 4073 1937
Size: 427 1709 4594
Fragments arranged by size:
4594 1709 427
Acc65I G'GTAC_C
Cuts at: 2526 4051 2526
Size: 1525 5205
AclI AA'CG_TT
Cuts at: 1563 4857 5230 1563
Size: 3294 373 3063
Fragments arranged by size:
3294 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2463 2576 2786 2882 2896 3355 4793 6177
Size: 831 113 210 96 14 459 1438 1384
Cuts at: 6177 6445 1632
Size: 268 1917
Fragments arranged by size:
1917 1438 1384 831 459 268 210 113
96 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6533
AflIII A'CryG_T
Cuts at: 1203 2403 5629 6606 1203
Size: 1200 3226 977 1327
Fragments arranged by size:
3226 1327 1200 977
AgeI A'CCGG_T
Cuts at: 5561 5561
Size: 6730
AhdI GACnn_n'nnGTC
Cuts at: 5459 5459
Size: 6730
AleI CACnn'nnGTG
Cuts at: 1166 3275 1166
Size: 2109 4621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6730
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 2201
Size: 32 6698
AlwNI CAG_nnn'CTG
Cuts at: 2101 2567 2764 3043 3082 3253 3365 3485 4473
Size: 466 197 279 39 171 112 120 988
Cuts at: 4473 6045 2101
Size: 1572 2786
Fragments arranged by size:
2786 1572 988 466 279 197 171 120
112 39
ApaLI G'TGCA_C
Cuts at: 3268 4787 5943 3268
Size: 1519 1156 4055
Fragments arranged by size:
4055 1519 1156
ApoI r'AATT_y
Cuts at: 1493 2330 2807 3005 4039 4265 4417 6526 1493
Size: 837 477 198 1034 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 837 477 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 5284 160
Size: 872 4252 1606
Fragments arranged by size:
4252 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
AvaI C'yCGr_G
Cuts at: 1870 1943 2942 3588 4055 1870
Size: 73 999 646 467 4545
Fragments arranged by size:
4545 999 646 467 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3686 3719 3686
Size: 33 6697
BamHI G'GATC_C
Cuts at: 4060 4427 4060
Size: 367 6363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2461 2526 4051 5506 618
Size: 325 217 627 674 65 1525 1455 1842
Fragments arranged by size:
1842 1525 1455 674 627 325 217 65
BanII G_rGCy'C
Cuts at: 1391 2118 3222 3732 3783 3994 4001 4049 1391
Size: 727 1104 510 51 211 7 48 4072
Fragments arranged by size:
4072 1104 727 510 211 51 48 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2747 2805 928
Size: 971 848 58 4853
Fragments arranged by size:
4853 971 848 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2933 2967 3183 3217 4921 4955 1903
Size: 34 996 34 216 34 1704 34 3678
Fragments arranged by size:
3678 1704 996 216 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4622 5832 4622
Size: 1210 5520
BclI T'GATC_A
Cuts at: 1548 3084 1548
Size: 1536 5194
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5341 136
Size: 107 122 71 631 605 3669 1525
Fragments arranged by size:
3669 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2203 6725 2203
Size: 4522 2208
BlpI GC'TnA_GC
Cuts at: 2753 3216 4147 2753
Size: 463 931 5336
Fragments arranged by size:
5336 931 463
Bme1580I G_kGCm'C
Cuts at: 1790 3272 3801 4791 5947 1790
Size: 1482 529 990 1156 2573
Fragments arranged by size:
2573 1482 1156 990 529
BmgBI CAC'GTC
Cuts at: 1318 2620 3355 1318
Size: 1302 735 4693
Fragments arranged by size:
4693 1302 735
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3404 3573 3711
Size: 416 53 83 399 24 1987 169 138
Cuts at: 3711 3995 4461 5419 442
Size: 284 466 958 1753
Fragments arranged by size:
1987 1753 958 466 416 399 284 169
138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6730
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 2299 2050
Size: 249 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2135 2168 2573 2597 3161 3269 3920
Size: 781 115 33 405 24 564 108 651
Cuts at: 3920 4036 5390 1239
Size: 116 1354 2579
Fragments arranged by size:
2579 1354 781 651 564 405 116 115
108 33 24
Bpu10I CC'TnA_GC
Cuts at: 3509 3509
Size: 6730
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 4855 5720 6018 3011
Size: 1844 865 298 3723
Fragments arranged by size:
3723 1844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2137 2973 3425 5393 882
Size: 617 638 836 452 1968 2219
Fragments arranged by size:
2219 1968 836 638 617 452
BsaAI yAC'GTr
Cuts at: 493 1204 2406 4521 493
Size: 711 1202 2115 2702
Fragments arranged by size:
2702 2115 1202 711
BsaBI GATnn'nnATC
Cuts at: 6724 6724
Size: 6730
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4537 4919 5554 275
Size: 53 83 186 936 3004 382 635 1451
Fragments arranged by size:
3004 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2050 2409 3775 5163 5561 5835 5982 6393 2050
Size: 359 1366 1388 398 274 147 411 2387
Fragments arranged by size:
2387 1388 1366 411 398 359 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2717 2747 3456 3486 1178
Size: 30 165 30 1314 30 709 30 4422
Fragments arranged by size:
4422 1314 709 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 3182 3740 1186
Size: 1168 828 558 4176
Fragments arranged by size:
4176 1168 828 558
BseYI C'CCAG_C
Cuts at: 3253 3336 3686 3782 3787 5933 6429 3253
Size: 83 350 96 5 2146 496 3554
Fragments arranged by size:
3554 2146 496 350 96 83 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 2978 3067 3467 3518 3741 1163
Size: 573 588 654 89 400 51 223 4152
Fragments arranged by size:
4152 654 588 573 400 223 89 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4180 4941 5969 1540
Size: 275 186 1642 537 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 537 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3222 3272 3732 4791 4876 5947 2118
Size: 1104 50 460 1059 85 1071 2901
Fragments arranged by size:
2901 1104 1071 1059 460 85 50
BsmI GAATG_Cn'
Cuts at: 4241 4334 4241
Size: 93 6637
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 3222 3272 3732 3783 3801 3994
Size: 399 328 1104 50 460 51 18 193
Cuts at: 3994 4001 4049 4791 4876 5947 1391
Size: 7 48 742 85 1071 2174
Fragments arranged by size:
2174 1104 1071 742 460 399 328 193
85 51 50 48 18 7
BspEI T'CCGG_A
Cuts at: 2050 6393 2050
Size: 4343 2387
BspHI T'CATG_A
Cuts at: 4619 6349 4619
Size: 1730 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3504 4085 844
Size: 2660 581 3489
Fragments arranged by size:
3489 2660 581
BsrBI CCG'CTC
Cuts at: 1394 1823 2704 3121 4617 1394
Size: 429 881 417 1496 3507
Fragments arranged by size:
3507 1496 881 429 417
BsrDI GCAATG_nn'
Cuts at: 65 1248 5225 5399 65
Size: 1183 3977 174 1396
Fragments arranged by size:
3977 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3682 5374 5561 1700
Size: 110 1872 1692 187 2869
Fragments arranged by size:
2869 1872 1692 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6730
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6730
BssSI C'ACGA_G
Cuts at: 4790 5802 4790
Size: 1012 5718
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3310 794
Size: 2516 4214
BstBI TT'CG_AA
Cuts at: 5534 5534
Size: 6730
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6730
BstXI CCAn_nnnn'nTGG
Cuts at: 4008 4008
Size: 6730
BstYI r'GATC_y
Cuts at: 1972 2203 2474 4060 4427 4812 4829 6281 6367
Size: 231 271 1586 367 385 17 1452 86
Cuts at: 6367 6379 6725 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1586 1452 385 367 346 271 231
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5239
Fragments arranged by size:
5239 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5991
Fragments arranged by size:
5991 683 56
BtsI GCAGTG_nn'
Cuts at: 2531 2961 3157 3254 4246 5040 5060 2531
Size: 430 196 97 992 794 20 4201
Fragments arranged by size:
4201 992 794 430 196 97 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6730
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3538 3573 529
Size: 35 2974 35 3686
Fragments arranged by size:
3686 2974 35 35
DraI TTT'AAA
Cuts at: 4034 4034
Size: 6730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2129 5737 809
Size: 507 813 3608 1802
Fragments arranged by size:
3608 1802 813 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3054 3603
Size: 54 1425 50 275 18 177 1047 549
Cuts at: 3603 3640 3846 3984 4177 5066 6695 8
Size: 37 206 138 193 889 1629 43
Fragments arranged by size:
1629 1425 1047 889 549 275 206 193
177 138 54 50 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 4177 1537
Size: 275 1828 537 4090
Fragments arranged by size:
4090 1828 537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 2817 3123 4660 1353
Size: 933 531 306 1537 3423
Fragments arranged by size:
3423 1537 933 531 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5317 5691 5837 387
Size: 1064 138 3728 374 146 1280
Fragments arranged by size:
3728 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2116 3220 3730 2116
Size: 1104 510 5116
Fragments arranged by size:
5116 1104 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2135 2168 2463 2573 2576 2597
Size: 393 388 115 33 295 110 3 21
Cuts at: 2597 2786 2882 2896 3161 3269 3355 3920 4036
Size: 189 96 14 265 108 86 565 116
Cuts at: 4036 4793 5390 6177 6445 1239
Size: 757 597 787 268 1524
Fragments arranged by size:
1524 787 757 597 565 393 388 295
268 265 189 116 115 110 108 96
86 33 21 14 3
EcoO109I rG'GnC_Cy
Cuts at: 2067 2948 3292 2067
Size: 881 344 5505
Fragments arranged by size:
5505 881 344
EcoRI G'AATT_C
Cuts at: 2330 4039 4417 2330
Size: 1709 378 4643
Fragments arranged by size:
4643 1709 378
EcoRV GAT'ATC
Cuts at: 2236 2236
Size: 6730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3083 3083
Size: 6730
FspI TGC'GCA
Cuts at: 2651 5236 2651
Size: 2585 4145
HaeII r_GCGC'y
Cuts at: 1678 5877 1678
Size: 4199 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2066 2098 3553
Size: 32 135 32 443 32 19 32 1455
Cuts at: 3553 3571 3585 3603 5371 5403 5445 5477 1373
Size: 18 14 18 1768 32 42 32 2626
Fragments arranged by size:
2626 1768 1455 443 135 42 32 32
32 32 32 32 19 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4074 4320 669
Size: 988 281 2136 246 3079
Fragments arranged by size:
3079 2136 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6730
HpaI GTT'AAC
Cuts at: 4320 4320
Size: 6730
KpnI G_GTAC'C
Cuts at: 2530 4055 2530
Size: 1525 5205
MfeI C'AATT_G
Cuts at: 4329 4329
Size: 6730
MluI A'CGCG_T
Cuts at: 6606 6606
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2325 5844 6028 1305
Size: 1020 3519 184 2007
Fragments arranged by size:
3519 2007 1020 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3056 3605 3848 3986
Size: 54 1425 343 177 1047 549 243 138
Cuts at: 3986 10
Size: 2754
Fragments arranged by size:
2754 1425 1047 549 343 243 177 138
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 3275 4688 5047 5206 518
Size: 648 1077 1032 1413 359 159 2042
Fragments arranged by size:
2042 1413 1077 1032 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2431 2689 2761 3238 3340 3639 3645 3700
Size: 825 258 72 477 102 299 6 55
Cuts at: 3700 4146 4823 5971 6216 1606
Size: 446 677 1148 245 2120
Fragments arranged by size:
2120 1148 825 677 477 446 299 258
245 102 72 55 6
NaeI GCC'GGC
Cuts at: 3684 3684
Size: 6730
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5239
Fragments arranged by size:
5239 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6730
NgoMIV G'CCGG_C
Cuts at: 3682 3682
Size: 6730
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6730
NotI GC'GGCC_GC
Cuts at: 3640 4177 3640
Size: 537 6193
NspI r_CATG'y
Cuts at: 1132 1229 2149 4088 5633 1132
Size: 97 920 1939 1545 2229
Fragments arranged by size:
2229 1939 1545 920 97
PasI CC'CwG_GG
Cuts at: 1348 3524 1348
Size: 2176 4554
PciI A'CATG_T
Cuts at: 5629 5629
Size: 6730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3601 1108
Size: 2493 4237
PfoI T'CCnGG_A
Cuts at: 2109 2550 2613 2697 3897 2109
Size: 441 63 84 1200 4942
Fragments arranged by size:
4942 1200 441 84 63
PmeI GTTT'AAAC
Cuts at: 4034 4034
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4799 4831 6312 6344 4799
Size: 32 1481 32 5185
Fragments arranged by size:
5185 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3292 3292
Size: 6730
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6730
PsiI TTA'TAA
Cuts at: 4300 4300
Size: 6730
PstI C_TGCA'G
Cuts at: 830 2274 2430 2514 2544 2898 2956 3315 3876
Size: 1444 156 84 30 354 58 359 561
Cuts at: 3876 4082 830
Size: 206 3478
Fragments arranged by size:
3478 1444 561 359 354 206 156 84
58 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6730
PvuII CAG'CTG
Cuts at: 2689 2761 3238 3340 3700 2689
Size: 72 477 102 360 5719
Fragments arranged by size:
5719 477 360 102 72
SacI G_AGCT'C
Cuts at: 2118 3222 3732 2118
Size: 1104 510 5116
Fragments arranged by size:
5116 1104 510
SalI G'TCGA_C
Cuts at: 1936 4072 1936
Size: 2136 4594
SbfI CC_TGCA'GG
Cuts at: 3876 4082 3876
Size: 206 6524
ScaI AGT'ACT
Cuts at: 4978 6690 4978
Size: 1712 5018
SexAI A'CCwGG_T
Cuts at: 2522 3537 3819 2522
Size: 1015 282 5433
Fragments arranged by size:
5433 1015 282
SfcI C'TryA_G
Cuts at: 826 1045 2270 2426 2510 2540 2894 2952 3311
Size: 219 1225 156 84 30 354 58 359
Cuts at: 3311 3695 3872 4078 5213 5894 6085 826
Size: 384 177 206 1135 681 191 1471
Fragments arranged by size:
1471 1225 1135 681 384 359 354 219
206 191 177 156 84 58 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6730
SmaI CCC'GGG
Cuts at: 1872 4057 1872
Size: 2185 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2990 4834 5735 5997 820
Size: 197 926 1047 1844 901 262 1553
Fragments arranged by size:
1844 1553 1047 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4521 493
Size: 4028 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6730
SphI G_CATG'C
Cuts at: 1132 4088 1132
Size: 2956 3774
SspI AAT'ATT
Cuts at: 5 52 4654 5
Size: 47 4602 2081
Fragments arranged by size:
4602 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 2484 2996 3027 1517
Size: 870 97 512 31 5220
Fragments arranged by size:
5220 870 512 97 31
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 2676 4169 513
Size: 552 939 282 390 1493 3074
Fragments arranged by size:
3074 1493 939 552 390 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2827 4757 4774 4927 5112 5557 325
Size: 1255 1247 1930 17 153 185 445 1498
Fragments arranged by size:
1930 1498 1255 1247 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2640 2670 4976
Size: 274 80 33 51 1443 663 30 2306
Cuts at: 4976 6688 96
Size: 1712 138
Fragments arranged by size:
2306 1712 1443 663 274 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2717 2749 3456 3488 6690 6722 2717
Size: 32 707 32 3202 32 2725
Fragments arranged by size:
3202 2725 707 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6730
XbaI T'CTAG_A
Cuts at: 4066 4066
Size: 6730
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6730
XmaI C'CCGG_G
Cuts at: 1870 4055 1870
Size: 2185 4545
XmnI GAAnn'nnTTC
Cuts at: 4859 4859
Size: 6730
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4538 276
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI