Restriction map for
pFN21AE4188
(Circular) (Six-base) MAPSORT of: pFN21AE4188.seq Check: 7909 from: 1 to: 6727
pFN21AE4188 6727 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2506 4537 278
Size: 53 83 186 936 970 2031 2468
Fragments arranged by size:
2468 2031 970 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2382 3612 4070 1937
Size: 445 1230 458 4594
Fragments arranged by size:
4594 1230 458 445
Acc65I G'GTAC_C
Cuts at: 3800 4048 3800
Size: 248 6479
AclI AA'CG_TT
Cuts at: 1563 4854 5227 1563
Size: 3291 373 3063
Fragments arranged by size:
3291 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2838 3539 3554 3918 4790 6174 6442 1632
Size: 1206 701 15 364 872 1384 268 1917
Fragments arranged by size:
1917 1384 1206 872 701 364 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6530
AflIII A'CryG_T
Cuts at: 1203 5626 6603 1203
Size: 4423 977 1327
Fragments arranged by size:
4423 1327 977
AgeI A'CCGG_T
Cuts at: 5558 5558
Size: 6727
AhdI GACnn_n'nnGTC
Cuts at: 2362 3967 5456 2362
Size: 1605 1489 3633
Fragments arranged by size:
3633 1605 1489
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3273 1184
Size: 2089 4638
AlwNI CAG_nnn'CTG
Cuts at: 2671 2743 3016 3965 4470 6042 2671
Size: 72 273 949 505 1572 3356
Fragments arranged by size:
3356 1572 949 505 273 72
ApaI G_GGCC'C
Cuts at: 2899 2899
Size: 6727
ApaLI G'TGCA_C
Cuts at: 4784 5940 4784
Size: 1156 5571
ApoI r'AATT_y
Cuts at: 1493 4036 4262 4414 6523 1493
Size: 2543 226 152 2109 1697
Fragments arranged by size:
2543 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3792 5281 160
Size: 872 2760 1489 1606
Fragments arranged by size:
2760 1606 1489 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 3091 4052 1870
Size: 73 70 1078 961 4545
Fragments arranged by size:
4545 1078 961 73 70
BamHI G'GATC_C
Cuts at: 4057 4424 4057
Size: 367 6360
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 3800 4048 5503 618
Size: 325 217 627 1151 862 248 1455 1842
Fragments arranged by size:
1842 1455 1151 862 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2899 4046 1391
Size: 1508 1147 4072
Fragments arranged by size:
4072 1508 1147
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 2779 3556 928
Size: 971 126 754 777 4099
Fragments arranged by size:
4099 971 777 754 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4918 4952 1903
Size: 34 50 34 2897 34 3678
Fragments arranged by size:
3678 2897 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4619 5829 4619
Size: 1210 5517
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6727
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2153 5338 136
Size: 107 122 71 631 605 481 3185 1525
Fragments arranged by size:
3185 1525 631 605 481 122 107 71
BglII A'GATC_T
Cuts at: 2650 3576 6722 2650
Size: 926 3146 2655
Fragments arranged by size:
3146 2655 926
BlpI GC'TnA_GC
Cuts at: 3201 4144 3201
Size: 943 5784
Bme1580I G_kGCm'C
Cuts at: 1790 2899 4788 5944 1790
Size: 1109 1889 1156 2573
Fragments arranged by size:
2573 1889 1156 1109
BmgBI CAC'GTC
Cuts at: 1318 2701 1318
Size: 1383 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 2766 3026
Size: 416 53 83 399 24 1158 191 260
Cuts at: 3026 3593 3614 4458 5416 442
Size: 567 21 844 958 1753
Fragments arranged by size:
1753 1158 958 844 567 416 399 260
191 83 53 24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6727
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3979 3476
Size: 503 6224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2765 3366 5387 1239
Size: 1526 601 2021 2579
Fragments arranged by size:
2579 2021 1526 601
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2519 4852 5717 6015 2147
Size: 372 2333 865 298 2859
Fragments arranged by size:
2859 2333 865 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3327 3662 3667 3774 5390 882
Size: 617 1828 335 5 107 1616 2219
Fragments arranged by size:
2219 1828 1616 617 335 107 5
BsaAI yAC'GTr
Cuts at: 493 1204 4518 493
Size: 711 3314 2702
Fragments arranged by size:
3314 2702 711
BsaBI GATnn'nnATC
Cuts at: 3041 6721 3041
Size: 3680 3047
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2216 2358 2503
Size: 53 83 186 936 489 194 142 145
Cuts at: 2503 4534 4916 5551 275
Size: 2031 382 635 1451
Fragments arranged by size:
2031 1451 936 635 489 382 194 186
145 142 83 53
BsaWI w'CCGG_w
Cuts at: 5160 5558 5832 5979 6390 5160
Size: 398 274 147 411 5497
Fragments arranged by size:
5497 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2429 2450 2459 1178
Size: 30 165 30 1017 9 21 9 5446
Fragments arranged by size:
5446 1017 165 30 30 21 9 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 3587 1186
Size: 1465 936 4326
Fragments arranged by size:
4326 1465 936
BseYI C'CCAG_C
Cuts at: 2664 5930 6426 2664
Size: 3266 496 2965
Fragments arranged by size:
3266 2965 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2506 2756 1163
Size: 573 770 250 5134
Fragments arranged by size:
5134 770 573 250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4177 4938 5966 1540
Size: 275 186 2176 761 1028 2301
Fragments arranged by size:
2301 2176 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4788 4873 5944 4788
Size: 85 1071 5571
Fragments arranged by size:
5571 1071 85
BsmI GAATG_Cn'
Cuts at: 3374 4238 4331 3374
Size: 864 93 5770
Fragments arranged by size:
5770 864 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2899 4046 4788 4873 5944 1391
Size: 399 1109 1147 742 85 1071 2174
Fragments arranged by size:
2174 1147 1109 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6390 6390
Size: 6727
BspHI T'CATG_A
Cuts at: 3603 4616 6346 3603
Size: 1013 1730 3984
Fragments arranged by size:
3984 1730 1013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4082 844
Size: 3238 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2764 4614 1394
Size: 429 941 1850 3507
Fragments arranged by size:
3507 1850 941 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2269 3547 5222 5396 65
Size: 1183 1021 1278 1675 174 1396
Fragments arranged by size:
1675 1396 1278 1183 1021 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5371 5558 1700
Size: 110 3561 187 2869
Fragments arranged by size:
3561 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2925 96
Size: 2829 3898
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6727
BssSI C'ACGA_G
Cuts at: 3852 4787 5799 3852
Size: 935 1012 4780
Fragments arranged by size:
4780 1012 935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6727
BstBI TT'CG_AA
Cuts at: 5531 5531
Size: 6727
BstEII G'GTnAC_C
Cuts at: 1184 2915 1184
Size: 1731 4996
BstXI CCAn_nnnn'nTGG
Cuts at: 2209 3037 2209
Size: 828 5899
BstYI r'GATC_y
Cuts at: 1972 2650 3064 3576 4057 4424 4809 4826 6278
Size: 678 414 512 481 367 385 17 1452
Cuts at: 6278 6364 6376 6722 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 678 512 481 414 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4023 4023
Size: 6727
BtgI C'CryG_G
Cuts at: 513 1065 2053 2274 513
Size: 552 988 221 4966
Fragments arranged by size:
4966 988 552 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2543 487
Size: 683 56 1317 4671
Fragments arranged by size:
4671 1317 683 56
BtsI GCAGTG_nn'
Cuts at: 2739 2744 3410 4243 5037 5057 2739
Size: 5 666 833 794 20 4409
Fragments arranged by size:
4409 833 794 666 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6692
DraI TTT'AAA
Cuts at: 4031 4031
Size: 6727
DraIII CAC_nnn'GTG
Cuts at: 2353 2353
Size: 6727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5734 809
Size: 507 4418 1802
Fragments arranged by size:
4418 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2554 3697
Size: 54 1425 50 275 18 441 283 1143
Cuts at: 3697 4174 5063 6692 8
Size: 477 889 1629 43
Fragments arranged by size:
1629 1425 1143 889 477 441 283 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4174 1537
Size: 275 2362 4090
Fragments arranged by size:
4090 2362 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4657 1353
Size: 3304 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5314 5688 5834 387
Size: 1064 138 3725 374 146 1280
Fragments arranged by size:
3725 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2765 2838 3366 3539 3554 3918 4790
Size: 393 1133 73 528 173 15 364 872
Cuts at: 4790 5387 6174 6442 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1133 872 787 597 528 393 364
268 173 73 15
EcoO109I rG'GnC_Cy
Cuts at: 2768 2895 2896 3097 2768
Size: 127 1 201 6398
Fragments arranged by size:
6398 201 127 1
EcoRI G'AATT_C
Cuts at: 4036 4414 4036
Size: 378 6349
EcoRV GAT'ATC
Cuts at: 3595 3595
Size: 6727
FspI TGC'GCA
Cuts at: 5233 5233
Size: 6727
HaeII r_GCGC'y
Cuts at: 1678 2434 4022 5874 1678
Size: 756 1588 1852 2531
Fragments arranged by size:
2531 1852 1588 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 3074 3106 3953
Size: 32 135 32 776 32 694 32 847
Cuts at: 3953 3985 5368 5400 5442 5474 1373
Size: 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 847 776 694 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 4071 4317 669
Size: 988 281 445 1688 246 3079
Fragments arranged by size:
3079 1688 988 445 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6727
HpaI GTT'AAC
Cuts at: 4317 4317
Size: 6727
KpnI G_GTAC'C
Cuts at: 3804 4052 3804
Size: 248 6479
MfeI C'AATT_G
Cuts at: 4326 4326
Size: 6727
MluI A'CGCG_T
Cuts at: 6603 6603
Size: 6727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 5841 6025 1305
Size: 1167 3369 184 2007
Fragments arranged by size:
3369 2007 1167 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2556 3699 10
Size: 54 1425 343 724 1143 3038
Fragments arranged by size:
3038 1425 1143 724 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 3290 4685 5044 5203 518
Size: 648 1041 1083 1395 359 159 2042
Fragments arranged by size:
2042 1395 1083 1041 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2226 2276 2461 2668 4143 4820 5968 6213
Size: 620 50 185 207 1475 677 1148 245
Cuts at: 6213 1606
Size: 2120
Fragments arranged by size:
2120 1475 1148 677 620 245 207 185
50
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6175
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6727
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6727
NotI GC'GGCC_GC
Cuts at: 4174 4174
Size: 6727
NspI r_CATG'y
Cuts at: 1132 1229 2283 2302 2317 2455 3487 4085 5630
Size: 97 1054 19 15 138 1032 598 1545
Cuts at: 5630 1132
Size: 2229
Fragments arranged by size:
2229 1545 1054 1032 598 138 97 19
15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6727
PciI A'CATG_T
Cuts at: 5626 5626
Size: 6727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 3016 1108
Size: 1563 345 4819
Fragments arranged by size:
4819 1563 345
PmeI GTTT'AAAC
Cuts at: 4031 4031
Size: 6727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4796 4828 6309 6341 4796
Size: 32 1481 32 5182
Fragments arranged by size:
5182 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2768 3097 2768
Size: 329 6398
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6727
PsiI TTA'TAA
Cuts at: 4297 4297
Size: 6727
PspOMI G'GGCC_C
Cuts at: 2895 2895
Size: 6727
PstI C_TGCA'G
Cuts at: 830 2146 2782 2904 2995 4079 830
Size: 1316 636 122 91 1084 3478
Fragments arranged by size:
3478 1316 1084 636 122 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6727
PvuII CAG'CTG
Cuts at: 2461 2668 2461
Size: 207 6520
SacII CC_GC'GG
Cuts at: 2277 2277
Size: 6727
SalI G'TCGA_C
Cuts at: 1936 2381 4069 1936
Size: 445 1688 4594
Fragments arranged by size:
4594 1688 445
SanDI GG'GwC_CC
Cuts at: 2768 2768
Size: 6727
SbfI CC_TGCA'GG
Cuts at: 4079 4079
Size: 6727
ScaI AGT'ACT
Cuts at: 2873 4975 6687 2873
Size: 2102 1712 2913
Fragments arranged by size:
2913 2102 1712
SexAI A'CCwGG_T
Cuts at: 3877 3877
Size: 6727
SfcI C'TryA_G
Cuts at: 826 1045 2142 2456 2778 2900 2991 3679 4075
Size: 219 1097 314 322 122 91 688 396
Cuts at: 4075 5210 5891 6082 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1097 688 681 396 322 314
219 191 122 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6727
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6727
SmaI CCC'GGG
Cuts at: 1872 2015 4054 1872
Size: 143 2039 4545
Fragments arranged by size:
4545 2039 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2534 4831 5732 5994 820
Size: 197 926 219 372 2297 901 262 1553
Fragments arranged by size:
2297 1553 926 901 372 262 219 197
SnaBI TAC'GTA
Cuts at: 493 4518 493
Size: 4025 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6727
SphI G_CATG'C
Cuts at: 1132 4085 1132
Size: 2953 3774
SspI AAT'ATT
Cuts at: 5 52 3652 4651 5
Size: 47 3600 999 2081
Fragments arranged by size:
3600 2081 999 47
StuI AGG'CCT
Cuts at: 1517 3813 1517
Size: 2296 4431
StyI C'CwwG_G
Cuts at: 513 1065 3030 3657 4166 513
Size: 552 1965 627 509 3074
Fragments arranged by size:
3074 1965 627 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3211 3997 4754 4771 4924 5109 5554
Size: 1255 1631 786 757 17 153 185 445
Cuts at: 5554 325
Size: 1498
Fragments arranged by size:
1631 1498 1255 786 757 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2871 2925 4973
Size: 274 80 33 51 1443 894 54 2048
Cuts at: 4973 6685 96
Size: 1712 138
Fragments arranged by size:
2048 1712 1443 894 274 138 80 54
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6687 6719 6687
Size: 32 6695
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6727
XbaI T'CTAG_A
Cuts at: 4063 4063
Size: 6727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 6727
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6727
XmaI C'CCGG_G
Cuts at: 1870 2013 4052 1870
Size: 143 2039 4545
Fragments arranged by size:
4545 2039 143
XmnI GAAnn'nnTTC
Cuts at: 4856 4856
Size: 6727
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2504 4535 276
Size: 53 83 186 936 970 2031 2468
Fragments arranged by size:
2468 2031 970 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SapI
SfiI SfoI SrfI SwaI