Restriction map for
pFN21AE4180
(Circular) (Six-base) MAPSORT of: pFN21AE4180.seq Check: 7114 from: 1 to: 6646
pFN21AE4180 6646 bp
With 182 enzymes: SgfI *
March 28, 2012 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2223 2223
Size: 6646
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2718 3750 4456 278
Size: 53 83 186 936 1182 1032 706 2468
Fragments arranged by size:
2468 1182 1032 936 706 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2499 3187 3782 3833 4709 6093 6361 1632
Size: 867 688 595 51 876 1384 268 1917
Fragments arranged by size:
1917 1384 876 867 688 595 268 51
AfeI AGC'GCT
Cuts at: 3086 3086
Size: 6646
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6449
AflIII A'CryG_T
Cuts at: 1203 3732 5545 6522 1203
Size: 2529 1813 977 1327
Fragments arranged by size:
2529 1813 1327 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 2217 3141 3872 1166
Size: 1051 924 731 3940
Fragments arranged by size:
3940 1051 924 731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3159 1184
Size: 1975 4671
AlwNI CAG_nnn'CTG
Cuts at: 2660 3687 3811 4389 5961 2660
Size: 1027 124 578 1572 3345
Fragments arranged by size:
3345 1572 1027 578 124
ApaI G_GGCC'C
Cuts at: 2012 2547 3835 2012
Size: 535 1288 4823
Fragments arranged by size:
4823 1288 535
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 3293 3461 3955 4181 4333 6442 1493
Size: 1800 168 494 226 152 2109 1697
Fragments arranged by size:
2109 1800 1697 494 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2546 2876 3971 1870
Size: 73 339 264 330 1095 4545
Fragments arranged by size:
4545 1095 339 330 264 73
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3013 3274 3560 3967 5422
Size: 325 217 627 1226 261 286 407 1455
Cuts at: 5422 618
Size: 1842
Fragments arranged by size:
1842 1455 1226 627 407 325 286 261
217
BanII G_rGCy'C
Cuts at: 1391 2012 2547 2829 3175 3482 3835 3965 1391
Size: 621 535 282 346 307 353 130 4072
Fragments arranged by size:
4072 621 535 353 346 307 282 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3460 928
Size: 971 1561 4114
Fragments arranged by size:
4114 1561 971
BbvCI CC'TCA_GC
Cuts at: 2555 2555
Size: 6646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4837 4871 1903
Size: 34 2900 34 3678
Fragments arranged by size:
3678 2900 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4538 5748 4538
Size: 1210 5436
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2301 2690 2988
Size: 107 122 71 631 605 629 389 298
Cuts at: 2988 3117 3794 5257 136
Size: 129 677 1463 1525
Fragments arranged by size:
1525 1463 677 631 629 605 389 298
129 122 107 71
BglII A'GATC_T
Cuts at: 6641 6641
Size: 6646
BlpI GC'TnA_GC
Cuts at: 3680 4063 3680
Size: 383 6263
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2547 3279 3563 3835 4707 5863 1790
Size: 222 535 732 284 272 872 1156 2573
Fragments arranged by size:
2573 1156 872 732 535 284 272 222
BmgBI CAC'GTC
Cuts at: 1318 3418 3591 1318
Size: 2100 173 4373
Fragments arranged by size:
4373 2100 173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 2480 2563
Size: 416 53 83 399 24 1041 22 83
Cuts at: 2563 4377 5335 442
Size: 1814 958 1753
Fragments arranged by size:
1814 1753 1041 958 416 399 83 83
53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2318 2682 2843 3109 3601 5306 1239
Size: 962 117 364 161 266 492 1705 2579
Fragments arranged by size:
2579 1705 962 492 364 266 161 117
Bpu10I CC'TnA_GC
Cuts at: 2555 2555
Size: 6646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4771 5636 5934 4771
Size: 865 298 5483
Fragments arranged by size:
5483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2873 3212 5309 882
Size: 617 1374 339 2097 2219
Fragments arranged by size:
2219 2097 1374 617 339
BsaAI yAC'GTr
Cuts at: 493 1204 2949 3139 4437 493
Size: 711 1745 190 1298 2702
Fragments arranged by size:
2702 1745 1298 711 190
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2044 2715 3372 3747
Size: 53 83 186 936 511 671 657 375
Cuts at: 3747 4453 4835 5470 275
Size: 706 382 635 1451
Fragments arranged by size:
1451 936 706 671 657 635 511 382
375 186 83 53
BsaWI w'CCGG_w
Cuts at: 2073 5079 5477 5751 5898 6309 2073
Size: 3006 398 274 147 411 2410
Fragments arranged by size:
3006 2410 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2951 2981 3436 3466 1178
Size: 30 165 30 1548 30 455 30 4358
Fragments arranged by size:
4358 1548 455 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 2609 2933 3038 3457 3737 1186
Size: 1312 111 324 105 419 280 4095
Fragments arranged by size:
4095 1312 419 324 280 111 105
BseYI C'CCAG_C
Cuts at: 2062 2245 2735 2883 3411 3822 5849 6345 2062
Size: 183 490 148 528 411 2027 496 2363
Fragments arranged by size:
2363 2027 528 496 490 411 183 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2816 3119 1163
Size: 573 1080 303 4690
Fragments arranged by size:
4690 1080 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 4096 4857 5885 1540
Size: 275 186 58 2037 761 1028 2301
Fragments arranged by size:
2301 2037 1028 761 275 186 58
BsiHKAI G_wGCw'C
Cuts at: 2115 3000 3711 4707 4792 5863 2115
Size: 885 711 996 85 1071 2898
Fragments arranged by size:
2898 1071 996 885 711 85
BsmI GAATG_Cn'
Cuts at: 4157 4250 4157
Size: 93 6553
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2177
Size: 6646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2115 2547 2829 3000 3175 3279
Size: 399 222 103 432 282 171 175 104
Cuts at: 3279 3482 3563 3711 3835 3965 4707 4792 5863
Size: 203 81 148 124 130 742 85 1071
Cuts at: 5863 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 432 399 282 222 203
175 171 148 130 124 104 103 85
81
BspEI T'CCGG_A
Cuts at: 2073 6309 2073
Size: 4236 2410
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 4001 844
Size: 1379 1778 3489
Fragments arranged by size:
3489 1778 1379
BsrBI CCG'CTC
Cuts at: 1394 1823 3050 3552 4533 1394
Size: 429 1227 502 981 3507
Fragments arranged by size:
3507 1227 981 502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3653 5141 5315 65
Size: 1183 2405 1488 174 1396
Fragments arranged by size:
2405 1488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 2908 3146 3386 3781 5290 5477
Size: 110 248 850 238 240 395 1509 187
Cuts at: 5477 1700
Size: 2869
Fragments arranged by size:
2869 1509 850 395 248 240 238 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 3215 4706 5718 3215
Size: 1491 1012 4143
Fragments arranged by size:
4143 1491 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2839 3567 794
Size: 2045 728 3873
Fragments arranged by size:
3873 2045 728
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 3847 1184
Size: 2663 3983
BstXI CCAn_nnnn'nTGG
Cuts at: 3664 3859 3664
Size: 195 6451
BstYI r'GATC_y
Cuts at: 1972 2070 3807 3976 4343 4728 4745 6197 6283
Size: 98 1737 169 367 385 17 1452 86
Cuts at: 6283 6295 6641 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1737 1452 385 367 346 169 98
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2940 3615 3858 513
Size: 552 939 936 675 243 3301
Fragments arranged by size:
3301 939 936 675 552 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3172 487
Size: 683 56 1946 3961
Fragments arranged by size:
3961 1946 683 56
BtsI GCAGTG_nn'
Cuts at: 2849 3630 4162 4956 4976 2849
Size: 781 532 794 20 4519
Fragments arranged by size:
4519 794 781 532 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6611
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DraIII CAC_nnn'GTG
Cuts at: 2571 3198 2571
Size: 627 6019
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 2604 2705
Size: 54 1425 50 275 18 633 141 101
Cuts at: 2705 2943 3073 3384 3838 3876 4093 4982 6611
Size: 238 130 311 454 38 217 889 1629
Cuts at: 6611 8
Size: 43
Fragments arranged by size:
1629 1425 889 633 454 311 275 238
217 141 130 101 54 50 43 38
18
EagI C'GGCC_G
Cuts at: 1537 1812 4093 1537
Size: 275 2281 4090
Fragments arranged by size:
4090 2281 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2475 3233 4576 1353
Size: 1122 758 1343 3423
Fragments arranged by size:
3423 1343 1122 758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2519 5233 5607 5753 387
Size: 1064 138 930 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 930 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2318 2499 2682 2843 3109 3187
Size: 393 569 117 181 183 161 266 78
Cuts at: 3187 3601 3782 3833 4709 5306 6093 6361 1239
Size: 414 181 51 876 597 787 268 1524
Fragments arranged by size:
1524 876 787 597 569 414 393 268
266 183 181 181 161 117 78 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2787 3839 2787
Size: 1052 5594
EcoO109I rG'GnC_Cy
Cuts at: 2009 2474 2616 2872 3118 3796 3832 2009
Size: 465 142 256 246 678 36 4823
Fragments arranged by size:
4823 678 465 256 246 142 36
EcoRI G'AATT_C
Cuts at: 3293 3461 3955 4333 3293
Size: 168 494 378 5606
Fragments arranged by size:
5606 494 378 168
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 3088 3687 5793 1678
Size: 1410 599 2106 2531
Fragments arranged by size:
2531 2106 1410 599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2062 2094 2372 2404 3314
Size: 32 135 32 490 32 278 32 910
Cuts at: 3314 3346 5287 5319 5361 5393 1373
Size: 32 1941 32 42 32 2626
Fragments arranged by size:
2626 1941 910 490 278 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3990 4236 669
Size: 988 281 2052 246 3079
Fragments arranged by size:
3079 2052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 4245 4245
Size: 6646
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3942 5760 5944 1305
Size: 2637 1818 184 2007
Fragments arranged by size:
2637 2007 1818 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 2945 3075 3840 3878
Size: 54 1425 343 633 480 130 765 38
Cuts at: 3878 10
Size: 2778
Fragments arranged by size:
2778 1425 765 633 480 343 130 54
38
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 3141 3272 3872 4604 4963 5122
Size: 648 1051 924 131 600 732 359 159
Cuts at: 5122 518
Size: 2042
Fragments arranged by size:
2042 1051 924 732 648 600 359 159
131
MspA1I CmG'CkG
Cuts at: 1606 2149 2229 2245 2887 3310 4062 4739 5887
Size: 543 80 16 642 423 752 677 1148
Cuts at: 5887 6132 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 752 677 642 543 423 245
80 16
NaeI GCC'GGC
Cuts at: 2910 3148 3388 3783 2910
Size: 238 240 395 5773
Fragments arranged by size:
5773 395 240 238
NcoI C'CATG_G
Cuts at: 513 1065 2004 3858 513
Size: 552 939 1854 3301
Fragments arranged by size:
3301 1854 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NgoMIV G'CCGG_C
Cuts at: 2908 3146 3386 3781 2908
Size: 238 240 395 5773
Fragments arranged by size:
5773 395 240 238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 4004 5549 1132
Size: 97 2775 1545 2229
Fragments arranged by size:
2775 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2679 3828 1348
Size: 1331 1149 4166
Fragments arranged by size:
4166 1331 1149
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2660 2941 1108
Size: 1552 281 4813
Fragments arranged by size:
4813 1552 281
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PmlI CAC'GTG
Cuts at: 2949 3139 2949
Size: 190 6456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2474 2616 2872 2474
Size: 142 256 6248
Fragments arranged by size:
6248 256 142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PspOMI G'GGCC_C
Cuts at: 2008 2543 3831 2008
Size: 535 1288 4823
Fragments arranged by size:
4823 1288 535
PspXI vC'TCGA_Gb
Cuts at: 2282 2282
Size: 6646
PstI C_TGCA'G
Cuts at: 830 2154 2844 3109 3795 3998 830
Size: 1324 690 265 686 203 3478
Fragments arranged by size:
3478 1324 690 686 265 203
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
PvuII CAG'CTG
Cuts at: 2149 2245 2887 3310 2149
Size: 96 642 423 5485
Fragments arranged by size:
5485 642 423 96
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SbfI CC_TGCA'GG
Cuts at: 3795 3998 3795
Size: 203 6443
ScaI AGT'ACT
Cuts at: 4894 6606 4894
Size: 1712 4934
SfcI C'TryA_G
Cuts at: 826 1045 2150 2840 3105 3791 3994 5129 5810
Size: 219 1105 690 265 686 203 1135 681
Cuts at: 5810 6001 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1105 690 686 681 265 219
203 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3117 3117
Size: 6646
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2876 4750 5651 5913 820
Size: 197 926 339 594 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 594 339 262 197
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 4004 1132
Size: 2872 3774
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 3111 1517
Size: 1594 5052
StyI C'CwwG_G
Cuts at: 513 1065 2004 3543 3835 3858 4085 513
Size: 552 939 1539 292 23 227 3074
Fragments arranged by size:
3074 1539 939 552 292 227 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 4673 4690 4843 5028 5473 325
Size: 1255 1002 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 1002 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3006 4892 6604
Size: 274 80 33 51 1443 1029 1886 1712
Cuts at: 6604 96
Size: 138
Fragments arranged by size:
1886 1712 1443 1029 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2458 2490 2951 2983 3436 3468 3486 3518 6606
Size: 32 461 32 453 32 18 32 3088
Cuts at: 6606 6638 2458
Size: 32 2466
Fragments arranged by size:
3088 2466 461 453 32 32 32 32
32 18
Tth111I GACn'n_nGTC
Cuts at: 1316 2714 1316
Size: 1398 5248
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2686 3622 2242
Size: 444 936 5266
Fragments arranged by size:
5266 936 444
XhoI C'TCGA_G
Cuts at: 1943 2282 2876 1943
Size: 339 594 5713
Fragments arranged by size:
5713 594 339
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2716 3748 4454 276
Size: 53 83 186 936 1182 1032 706 2468
Fragments arranged by size:
2468 1182 1032 936 706 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PsrI RsrII SacI
SacII SanDI SapI SexAI SfoI SrfI SwaI