Restriction map for
pFN21AE4178
(Circular) (Six-base) MAPSORT of: pFN21AE4178.seq Check: 1586 from: 1 to: 6631
pFN21AE4178 6631 bp
With 182 enzymes: SgfI *
March 28, 2012 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2967 2967
Size: 6631
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3398 4441 278
Size: 53 83 186 936 1862 1043 2468
Fragments arranged by size:
2468 1862 1043 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2769 3974 1937
Size: 832 1205 4594
Fragments arranged by size:
4594 1205 832
Acc65I G'GTAC_C
Cuts at: 3952 3952
Size: 6631
AclI AA'CG_TT
Cuts at: 1563 4758 5131 1563
Size: 3195 373 3063
Fragments arranged by size:
3195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2739 2969 4694 6078 6346 1632
Size: 1107 230 1725 1384 268 1917
Fragments arranged by size:
1917 1725 1384 1107 268 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6434
AflIII A'CryG_T
Cuts at: 1203 2999 3246 3528 5530 6507 1203
Size: 1796 247 282 2002 977 1327
Fragments arranged by size:
2002 1796 1327 977 282 247
AgeI A'CCGG_T
Cuts at: 5462 5462
Size: 6631
AhdI GACnn_n'nnGTC
Cuts at: 5360 5360
Size: 6631
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2316 2645 1184
Size: 1132 329 5170
Fragments arranged by size:
5170 1132 329
AlwNI CAG_nnn'CTG
Cuts at: 2182 2497 2719 4374 5946 2182
Size: 315 222 1655 1572 2867
Fragments arranged by size:
2867 1655 1572 315 222
ApaI G_GGCC'C
Cuts at: 2070 2076 3145 2070
Size: 6 1069 5556
Fragments arranged by size:
5556 1069 6
ApaLI G'TGCA_C
Cuts at: 4688 5844 4688
Size: 1156 5475
ApoI r'AATT_y
Cuts at: 1493 3940 4166 4318 6427 1493
Size: 2447 226 152 2109 1697
Fragments arranged by size:
2447 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5185 160
Size: 872 4153 1606
Fragments arranged by size:
4153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2069 2075 2114 3144 3799 3956
Size: 73 119 7 6 39 1030 655 157
Cuts at: 3956 1870
Size: 4545
Fragments arranged by size:
4545 1030 655 157 119 73 39 7
6
AvrII C'CTAG_G
Cuts at: 2983 2983
Size: 6631
BamHI G'GATC_C
Cuts at: 3961 4328 3961
Size: 367 6264
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2910 2987 3593 3623
Size: 325 217 627 293 830 77 606 30
Cuts at: 3623 3685 3952 5407 618
Size: 62 267 1455 1842
Fragments arranged by size:
1842 1455 830 627 606 325 293 267
217 77 62 30
BanII G_rGCy'C
Cuts at: 1391 2048 2070 2076 3145 3519 3950 1391
Size: 657 22 6 1069 374 431 4072
Fragments arranged by size:
4072 1069 657 431 374 22 6
BbeI G_GCGC'C
Cuts at: 2084 3689 2084
Size: 1605 5026
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5660
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2526 2560 4822 4856 1903
Size: 34 50 34 505 34 2262 34 3678
Fragments arranged by size:
3678 2262 505 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2603 4523 5733 2603
Size: 1920 1210 3501
Fragments arranged by size:
3501 1920 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6631
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2031 2117 2193
Size: 107 122 71 631 605 359 86 76
Cuts at: 2193 2243 2268 2459 5242 136
Size: 50 25 191 2783 1525
Fragments arranged by size:
2783 1525 631 605 359 191 122 107
86 76 71 50 25
BglII A'GATC_T
Cuts at: 6626 6626
Size: 6631
BlpI GC'TnA_GC
Cuts at: 2183 2861 3554 3568 4048 2183
Size: 678 693 14 480 4766
Fragments arranged by size:
4766 693 678 480 14
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2076 3145 3275 3598 4692 5848 1790
Size: 280 6 1069 130 323 1094 1156 2573
Fragments arranged by size:
2573 1156 1094 1069 323 280 130 6
BmgBI CAC'GTC
Cuts at: 1318 2674 2803 3002 1318
Size: 1356 129 199 4947
Fragments arranged by size:
4947 1356 199 129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4362 5320 442
Size: 416 53 83 399 24 2945 958 1753
Fragments arranged by size:
2945 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3608 3687 3754 3890 5291 1239
Size: 2369 79 67 136 1401 2579
Fragments arranged by size:
2579 2369 1401 136 79 67
Bpu10I CC'TnA_GC
Cuts at: 2783 2967 2783
Size: 184 6447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 4756 5621 5919 2618
Size: 2138 865 298 3330
Fragments arranged by size:
3330 2138 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2734 5294 882
Size: 617 1235 2560 2219
Fragments arranged by size:
2560 2219 1235 617
BsaAI yAC'GTr
Cuts at: 493 1204 2296 2401 2653 2887 3115 3249 3604
Size: 711 1092 105 252 234 228 134 355
Cuts at: 3604 4422 493
Size: 818 2702
Fragments arranged by size:
2702 1092 818 711 355 252 234 228
134 105
BsaBI GATnn'nnATC
Cuts at: 6625 6625
Size: 6631
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2532 2808 2979
Size: 53 83 186 936 548 451 276 171
Cuts at: 2979 3395 3545 3686 4438 4820 5455 275
Size: 416 150 141 752 382 635 1451
Fragments arranged by size:
1451 936 752 635 548 451 416 382
276 186 171 150 141 83 53
BsaWI w'CCGG_w
Cuts at: 3383 5064 5462 5736 5883 6294 3383
Size: 1681 398 274 147 411 3720
Fragments arranged by size:
3720 1681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3329 3359 3467 3497 3752
Size: 30 165 30 1926 30 108 30 255
Cuts at: 3752 3782 1178
Size: 30 4027
Fragments arranged by size:
4027 1926 255 165 108 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 2774 2777 2924 3475 1186
Size: 1516 72 3 147 551 4342
Fragments arranged by size:
4342 1516 551 147 72 3
BseYI C'CCAG_C
Cuts at: 2503 2787 2878 5834 6330 2503
Size: 284 91 2956 496 2804
Fragments arranged by size:
2956 2804 496 284 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2276 2393 2465 2510 1163
Size: 573 459 81 117 72 45 5284
Fragments arranged by size:
5284 573 459 117 81 72 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 3106 3327 4081 4842 5870
Size: 275 186 30 1075 221 754 761 1028
Cuts at: 5870 1540
Size: 2301
Fragments arranged by size:
2301 1075 1028 761 754 275 221 186
30
BsiHKAI G_wGCw'C
Cuts at: 2055 3416 3710 4692 4777 5848 2055
Size: 1361 294 982 85 1071 2838
Fragments arranged by size:
2838 1361 1071 982 294 85
BsmI GAATG_Cn'
Cuts at: 4142 4235 4142
Size: 93 6538
BsmBI CGTCTCn'nnnn_
Cuts at: 2532 2532
Size: 6631
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2055 2070 2076 3145 3275 3416
Size: 399 258 7 15 6 1069 130 141
Cuts at: 3416 3519 3598 3710 3950 4692 4777 5848 1391
Size: 103 79 112 240 742 85 1071 2174
Fragments arranged by size:
2174 1071 1069 742 399 258 240 141
130 112 103 85 79 15 7 6
BspEI T'CCGG_A
Cuts at: 6294 6294
Size: 6631
BspHI T'CATG_A
Cuts at: 2835 4520 6250 2835
Size: 1685 1730 3216
Fragments arranged by size:
3216 1730 1685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2637 2967 3986 844
Size: 1793 330 1019 3489
Fragments arranged by size:
3489 1793 1019 330
BsrBI CCG'CTC
Cuts at: 1394 1823 4518 1394
Size: 429 2695 3507
Fragments arranged by size:
3507 2695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2491 5126 5300 65
Size: 1183 1243 2635 174 1396
Fragments arranged by size:
2635 1396 1243 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2107 3171 3326 5275 5462 1700
Size: 110 220 77 1064 155 1949 187 2869
Fragments arranged by size:
2869 1949 1064 220 187 155 110 77
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6631
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6631
BssSI C'ACGA_G
Cuts at: 4691 5703 4691
Size: 1012 5619
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2497 3630 794
Size: 1703 1133 3795
Fragments arranged by size:
3795 1703 1133
BstBI TT'CG_AA
Cuts at: 5435 5435
Size: 6631
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6631
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 2587
Size: 6631
BstYI r'GATC_y
Cuts at: 1972 2310 3961 4328 4713 4730 6182 6268 6280
Size: 338 1651 367 385 17 1452 86 12
Cuts at: 6280 6626 1972
Size: 346 1977
Fragments arranged by size:
1977 1651 1452 385 367 346 338 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2424 2424
Size: 6631
BtgI C'CryG_G
Cuts at: 513 1065 2160 2828 3070 3219 3267 3540 3726
Size: 552 1095 668 242 149 48 273 186
Cuts at: 3726 3747 513
Size: 21 3397
Fragments arranged by size:
3397 1095 668 552 273 242 186 149
48 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5892
Fragments arranged by size:
5892 683 56
BtsI GCAGTG_nn'
Cuts at: 3856 4147 4941 4961 3856
Size: 291 794 20 5526
Fragments arranged by size:
5526 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6596
DraI TTT'AAA
Cuts at: 3935 3935
Size: 6631
DraIII CAC_nnn'GTG
Cuts at: 2203 2326 2668 2803 3118 2203
Size: 123 342 135 315 5716
Fragments arranged by size:
5716 342 315 135 123
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5638 809
Size: 507 4322 1802
Fragments arranged by size:
4322 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2244 2676
Size: 54 1425 50 275 18 198 216 432
Cuts at: 2676 3297 3324 3438 3495 3579 3723 3819 4078
Size: 621 27 114 57 84 144 96 259
Cuts at: 4078 4967 6596 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 621 432 275 259 216
198 144 114 96 84 57 54 50
43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 3324 4078 1537
Size: 275 216 1296 754 4090
Fragments arranged by size:
4090 1296 754 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 4561 1353
Size: 3208 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5218 5592 5738 387
Size: 1064 138 3629 374 146 1280
Fragments arranged by size:
3629 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2739 2969 3608 3687 3754 3890 4694
Size: 393 1107 230 639 79 67 136 804
Cuts at: 4694 5291 6078 6346 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1107 804 787 639 597 393 268
230 136 79 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2633 2711 2939 2633
Size: 78 228 6325
Fragments arranged by size:
6325 228 78
EcoO109I rG'GnC_Cy
Cuts at: 2066 2929 2944 3201 3342 2066
Size: 863 15 257 141 5355
Fragments arranged by size:
5355 863 257 141 15
EcoRI G'AATT_C
Cuts at: 3940 4318 3940
Size: 378 6253
FspI TGC'GCA
Cuts at: 2319 5137 2319
Size: 2818 3813
FspAI rTGC'GCAy
Cuts at: 2319 2319
Size: 6631
HaeII r_GCGC'y
Cuts at: 1678 2026 2084 3013 3427 3689 5778 1678
Size: 348 58 929 414 262 2089 2531
Fragments arranged by size:
2531 2089 929 414 348 262 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 3188 3220 3329
Size: 32 135 32 1028 32 556 32 109
Cuts at: 3329 3361 3470 3502 3640 3672 3752 3784 5272
Size: 32 109 32 138 32 80 32 1488
Cuts at: 5272 5304 5346 5378 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 1028 556 138 135 109 109
80 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3975 4221 669
Size: 988 281 2037 246 3079
Fragments arranged by size:
3079 2037 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6631
HpaI GTT'AAC
Cuts at: 4221 4221
Size: 6631
KasI G'GCGC_C
Cuts at: 2080 3685 2080
Size: 1605 5026
KpnI G_GTAC'C
Cuts at: 3956 3956
Size: 6631
MfeI C'AATT_G
Cuts at: 4230 4230
Size: 6631
MluI A'CGCG_T
Cuts at: 6507 6507
Size: 6631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2604 5745 5929 1305
Size: 1299 3141 184 2007
Fragments arranged by size:
3141 2007 1299 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2246 2678 3440 3497 3725
Size: 54 1425 343 414 432 762 57 228
Cuts at: 3725 10
Size: 2916
Fragments arranged by size:
2916 1425 762 432 414 343 228 57
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2889 4589 4948 5107 518
Size: 648 1723 1700 359 159 2042
Fragments arranged by size:
2042 1723 1700 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2179 2440 2524 3108 3572 3630 4047 4724
Size: 573 261 84 584 464 58 417 677
Cuts at: 4724 5872 6117 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 584 573 464 417 261
245 84 58
NaeI GCC'GGC
Cuts at: 2032 2109 3328 2032
Size: 77 1219 5335
Fragments arranged by size:
5335 1219 77
NarI GG'CG_CC
Cuts at: 2081 3686 2081
Size: 1605 5026
NcoI C'CATG_G
Cuts at: 513 1065 2160 3267 3726 513
Size: 552 1095 1107 459 3418
Fragments arranged by size:
3418 1107 1095 552 459
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6631
NgoMIV G'CCGG_C
Cuts at: 2030 2107 3326 2030
Size: 77 1219 5335
Fragments arranged by size:
5335 1219 77
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6631
NotI GC'GGCC_GC
Cuts at: 4078 4078
Size: 6631
NspI r_CATG'y
Cuts at: 1132 1229 2260 2825 3835 3989 5534 1132
Size: 97 1031 565 1010 154 1545 2229
Fragments arranged by size:
2229 1545 1031 1010 565 154 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6631
PciI A'CATG_T
Cuts at: 5530 5530
Size: 6631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 3665 3813 1108
Size: 1095 1462 148 3926
Fragments arranged by size:
3926 1462 1095 148
PmeI GTTT'AAAC
Cuts at: 3935 3935
Size: 6631
PmlI CAC'GTG
Cuts at: 2296 2401 2887 3115 3249 2296
Size: 105 486 228 134 5678
Fragments arranged by size:
5678 486 228 134 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 6213 6245 4700
Size: 32 1481 32 5086
Fragments arranged by size:
5086 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2929 3201 2929
Size: 272 6359
PshAI GACnn'nnGTC
Cuts at: 1613 3398 1613
Size: 1785 4846
PsiI TTA'TAA
Cuts at: 4201 4201
Size: 6631
PspOMI G'GGCC_C
Cuts at: 2066 2072 3141 2066
Size: 6 1069 5556
Fragments arranged by size:
5556 1069 6
PstI C_TGCA'G
Cuts at: 830 2345 2523 2624 2661 3983 830
Size: 1515 178 101 37 1322 3478
Fragments arranged by size:
3478 1515 1322 178 101 37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6631
PvuII CAG'CTG
Cuts at: 2179 2440 2524 3630 2179
Size: 261 84 1106 5180
Fragments arranged by size:
5180 1106 261 84
SalI G'TCGA_C
Cuts at: 1936 3973 1936
Size: 2037 4594
SbfI CC_TGCA'GG
Cuts at: 3983 3983
Size: 6631
ScaI AGT'ACT
Cuts at: 4879 6591 4879
Size: 1712 4919
SexAI A'CCwGG_T
Cuts at: 3717 3717
Size: 6631
SfcI C'TryA_G
Cuts at: 826 1045 2341 2519 2620 2657 3979 5114 5795
Size: 219 1296 178 101 37 1322 1135 681
Cuts at: 5795 5986 826
Size: 191 1471
Fragments arranged by size:
1471 1322 1296 1135 681 219 191 178
101 37
SfoI GGC'GCC
Cuts at: 2082 3687 2082
Size: 1605 5026
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6631
SmaI CCC'GGG
Cuts at: 1872 2064 2071 3146 3958 1872
Size: 192 7 1075 812 4545
Fragments arranged by size:
4545 1075 812 192 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2633 4735 5636 5898 820
Size: 197 926 690 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 690 262 197
SnaBI TAC'GTA
Cuts at: 493 4422 493
Size: 3929 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6631
SphI G_CATG'C
Cuts at: 1132 2260 3989 1132
Size: 1128 1729 3774
Fragments arranged by size:
3774 1729 1128
SrfI GCCC'GGGC
Cuts at: 2071 2071
Size: 6631
SspI AAT'ATT
Cuts at: 5 52 4555 5
Size: 47 4503 2081
Fragments arranged by size:
4503 2081 47
StuI AGG'CCT
Cuts at: 1517 2429 3086 1517
Size: 912 657 5062
Fragments arranged by size:
5062 912 657
StyI C'CwwG_G
Cuts at: 513 1065 2160 2304 2430 2983 3267 3726 4070
Size: 552 1095 144 126 553 284 459 344
Cuts at: 4070 513
Size: 3074
Fragments arranged by size:
3074 1095 553 552 459 344 284 144
126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2699 2779 3281 3805 3845 4658 4675
Size: 1255 1119 80 502 524 40 813 17
Cuts at: 4675 4828 5013 5458 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1119 813 524 502 445 185
153 80 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3318 3792 4877
Size: 274 80 33 51 1443 1341 474 1085
Cuts at: 4877 6589 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1341 1085 474 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6591 6623 6591
Size: 32 6599
Tth111I GACn'n_nGTC
Cuts at: 1316 2285 3907 1316
Size: 969 1622 4040
Fragments arranged by size:
4040 1622 969
XbaI T'CTAG_A
Cuts at: 3967 3967
Size: 6631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3814 3814
Size: 6631
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6631
XmaI C'CCGG_G
Cuts at: 1870 2062 2069 3144 3956 1870
Size: 192 7 1075 812 4545
Fragments arranged by size:
4545 1075 812 192 7
XmnI GAAnn'nnTTC
Cuts at: 4760 4760
Size: 6631
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3396 4439 276
Size: 53 83 186 936 1862 1043 2468
Fragments arranged by size:
2468 1862 1043 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoRV FalI FseI NruI NsiI PacI
PfoI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SwaI