Restriction map for
pFN21AE4176
(Circular) (Six-base) MAPSORT of: pFN21AE4176.seq Check: 846 from: 1 to: 6619
pFN21AE4176 6619 bp
With 182 enzymes: SgfI *
March 28, 2012 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3403 3403
Size: 6619
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3962 1937
Size: 2025 4594
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2008 2183 2657 3000 3749 3929 4682 6066
Size: 376 175 474 343 749 180 753 1384
Cuts at: 6066 6334 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 753 749 474 376 343 268
180 175
AfeI AGC'GCT
Cuts at: 2576 2576
Size: 6619
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 2394 5518 6495 1203
Size: 1191 3124 977 1327
Fragments arranged by size:
3124 1327 1191 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3920 1184
Size: 2736 3883
AlwNI CAG_nnn'CTG
Cuts at: 2825 2860 3010 3193 3418 4362 5934 2825
Size: 35 150 183 225 944 1572 3510
Fragments arranged by size:
3510 1572 944 225 183 150 35
ApaI G_GGCC'C
Cuts at: 2258 2477 3882 2258
Size: 219 1405 4995
Fragments arranged by size:
4995 1405 219
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 3928 4154 4306 6415 1493
Size: 2435 226 152 2109 1697
Fragments arranged by size:
2435 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 2258 2354 2402 2570 3064 3227
Size: 73 308 7 96 48 168 494 163
Cuts at: 3227 3944 1870
Size: 717 4545
Fragments arranged by size:
4545 717 494 308 168 163 96 73
48 7
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2515 2647 3428 3443
Size: 325 217 627 518 210 132 781 15
Cuts at: 3443 3940 5395 618
Size: 497 1455 1842
Fragments arranged by size:
1842 1455 781 627 518 497 325 217
210 132 15
BanII G_rGCy'C
Cuts at: 1391 2184 2252 2258 2301 2477 2956 3075 3219
Size: 793 68 6 43 176 479 119 144
Cuts at: 3219 3525 3816 3882 3938 1391
Size: 306 291 66 56 4072
Fragments arranged by size:
4072 793 479 306 291 176 144 119
68 66 56 43 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3510 928
Size: 971 1611 4037
Fragments arranged by size:
4037 1611 971
BbvCI CC'TCA_GC
Cuts at: 3155 3155
Size: 6619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2714 2748 4810 4844 1903
Size: 34 777 34 2062 34 3678
Fragments arranged by size:
3678 2062 777 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2406 3543 3753
Size: 107 122 71 631 605 734 1137 210
Cuts at: 3753 5230 136
Size: 1477 1525
Fragments arranged by size:
1525 1477 1137 734 631 605 210 122
107 71
BglII A'GATC_T
Cuts at: 6614 6614
Size: 6619
BlpI GC'TnA_GC
Cuts at: 2682 3074 3213 4036 2682
Size: 392 139 823 5265
Fragments arranged by size:
5265 823 392 139
Bme1580I G_kGCm'C
Cuts at: 1790 2258 2402 2477 2552 3433 3882 4680 5836
Size: 468 144 75 75 881 449 798 1156
Cuts at: 5836 1790
Size: 2573
Fragments arranged by size:
2573 1156 881 798 468 449 144 75
75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6619
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2316 3463 4350
Size: 416 53 83 399 24 899 1147 887
Cuts at: 4350 5308 442
Size: 958 1753
Fragments arranged by size:
1753 1147 958 899 887 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3273 3273
Size: 6619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2675 3053 3632 3869 5279 1239
Size: 1436 378 579 237 1410 2579
Fragments arranged by size:
2579 1436 1410 579 378 237
Bpu10I CC'TnA_GC
Cuts at: 2699 2945 3155 2699
Size: 246 210 6163
Fragments arranged by size:
6163 246 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3260 3815 3869 4744 5609 5907 3260
Size: 555 54 875 865 298 3972
Fragments arranged by size:
3972 875 865 555 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5282 882
Size: 617 3783 2219
Fragments arranged by size:
3783 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4410 493
Size: 711 3206 2702
Fragments arranged by size:
3206 2702 711
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3861 4426 4808 5443
Size: 53 83 186 936 2328 565 382 635
Cuts at: 5443 275
Size: 1451
Fragments arranged by size:
2328 1451 936 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5052 5450 5724 5871 6282 5052
Size: 398 274 147 411 5389
Fragments arranged by size:
5389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 2582 2612 3026
Size: 30 165 30 1128 30 21 30 414
Cuts at: 3026 3056 3542 3572 1178
Size: 30 486 30 4225
Fragments arranged by size:
4225 1128 486 414 165 30 30 30
30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 2888 3928 1186
Size: 1366 336 1040 3877
Fragments arranged by size:
3877 1366 1040 336
BseYI C'CCAG_C
Cuts at: 2309 2728 2860 3418 3477 3541 5822 6318 2309
Size: 419 132 558 59 64 2281 496 2610
Fragments arranged by size:
2610 2281 558 496 419 132 64 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2799 3126 1163
Size: 573 1063 327 4656
Fragments arranged by size:
4656 1063 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2832 3532 3538 4069 4830 5858
Size: 275 186 831 700 6 531 761 1028
Cuts at: 5858 1540
Size: 2301
Fragments arranged by size:
2301 1028 831 761 700 531 275 186
6
BsiHKAI G_wGCw'C
Cuts at: 2631 3075 4680 4765 5836 2631
Size: 444 1605 85 1071 3414
Fragments arranged by size:
3414 1605 1071 444 85
BsmI GAATG_Cn'
Cuts at: 4130 4223 4130
Size: 93 6526
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2252 2258 2301 2402 2477 2552
Size: 399 394 68 6 43 101 75 75
Cuts at: 2552 2631 2956 3075 3219 3433 3525 3816 3882
Size: 79 325 119 144 214 92 291 66
Cuts at: 3882 3938 4680 4765 5836 1391
Size: 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 325 291 214
144 119 101 92 85 79 75 75
68 66 56 43 6
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 4508 6238 4508
Size: 1730 4889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2922 3403 3974 844
Size: 2078 481 571 3489
Fragments arranged by size:
3489 2078 571 481
BsrBI CCG'CTC
Cuts at: 1394 1823 2569 2794 2901 4506 1394
Size: 429 746 225 107 1605 3507
Fragments arranged by size:
3507 1605 746 429 225 107
BsrDI GCAATG_nn'
Cuts at: 65 1248 2739 5114 5288 65
Size: 1183 1491 2375 174 1396
Fragments arranged by size:
2375 1491 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2138 2529 2808 3537 5263 5450
Size: 110 249 79 391 279 729 1726 187
Cuts at: 5450 1700
Size: 2869
Fragments arranged by size:
2869 1726 729 391 279 249 187 110
79
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6619
BssHII G'CGCG_C
Cuts at: 1925 2106 2313 2665 2667 1925
Size: 181 207 352 2 5877
Fragments arranged by size:
5877 352 207 181 2
BssSI C'ACGA_G
Cuts at: 2480 2639 4679 5691 2480
Size: 159 2040 1012 3408
Fragments arranged by size:
3408 2040 1012 159
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2825 3010 794
Size: 2031 185 4403
Fragments arranged by size:
4403 2031 185
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 2909 1184
Size: 1725 4894
BstYI r'GATC_y
Cuts at: 1972 3949 4316 4701 4718 6170 6256 6268 6614
Size: 1977 367 385 17 1452 86 12 346
Cuts at: 6614 1972
Size: 1977
Fragments arranged by size:
1977 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2360 2367 2500 2632 2706 3126
Size: 552 939 356 7 133 132 74 420
Cuts at: 3126 3532 3642 513
Size: 406 110 3490
Fragments arranged by size:
3490 939 552 420 406 356 133 132
110 74 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 2020 3114 3578 4135 4929 4949 2020
Size: 1094 464 557 794 20 3690
Fragments arranged by size:
3690 1094 794 557 464 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2114 2564
Size: 54 1425 50 275 18 247 37 450
Cuts at: 2564 2635 2829 3123 3498 3535 4066 4955 6584
Size: 71 194 294 375 37 531 889 1629
Cuts at: 6584 8
Size: 43
Fragments arranged by size:
1629 1425 889 531 450 375 294 275
247 194 71 54 50 43 37 37
18
EagI C'GGCC_G
Cuts at: 1537 1812 2829 3535 4066 1537
Size: 275 1017 706 531 4090
Fragments arranged by size:
4090 1017 706 531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2370 3821 3905 4549 1353
Size: 1017 1451 84 644 3423
Fragments arranged by size:
3423 1451 1017 644 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2157 2231 5206 5580 5726 387
Size: 1064 138 568 74 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 568 374 146 138 74
EcoICRI GAG'CTC
Cuts at: 3073 3073
Size: 6619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2183 2657 2675 3000 3053 3632
Size: 393 376 175 474 18 325 53 579
Cuts at: 3632 3749 3869 3929 4682 5279 6066 6334 1239
Size: 117 120 60 753 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 579 474 393 376
325 268 175 120 117 60 53 18
EcoO109I rG'GnC_Cy
Cuts at: 2255 2974 3879 2255
Size: 719 905 4995
Fragments arranged by size:
4995 905 719
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3856 3856
Size: 6619
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 2578 2706 5766 1678
Size: 900 128 3060 2531
Fragments arranged by size:
3060 2531 900 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5260 5292 5334 5366 1373
Size: 32 135 32 3688 32 42 32 2626
Fragments arranged by size:
3688 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3139 3963 4209 669
Size: 988 281 1201 824 246 3079
Fragments arranged by size:
3079 1201 988 824 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6619
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 2394 6495 2394
Size: 4101 2518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5733 5917 1305
Size: 4428 184 2007
Fragments arranged by size:
4428 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4797
Fragments arranged by size:
4797 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3902 4577 4936 5095 518
Size: 648 2736 675 359 159 2042
Fragments arranged by size:
2736 2042 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2163 2170 2369 2502 2524 2634 2653
Size: 422 135 7 199 133 22 110 19
Cuts at: 2653 3154 3331 3534 4035 4712 5860 6105 1606
Size: 501 177 203 501 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 501 501 422 245 203
199 177 135 133 110 22 19 7
NaeI GCC'GGC
Cuts at: 2140 2531 2810 3539 2140
Size: 391 279 729 5220
Fragments arranged by size:
5220 729 391 279
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5128
Fragments arranged by size:
5128 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6619
NgoMIV G'CCGG_C
Cuts at: 2138 2529 2808 3537 2138
Size: 391 279 729 5220
Fragments arranged by size:
5220 729 391 279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 2829 4066 2829
Size: 1237 5382
NspI r_CATG'y
Cuts at: 1132 1229 3121 3977 5522 1132
Size: 97 1892 856 1545 2229
Fragments arranged by size:
2229 1892 1545 856 97
PasI CC'CwG_GG
Cuts at: 1348 2905 3050 3261 1348
Size: 1557 145 211 4706
Fragments arranged by size:
4706 1557 211 145
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 2860 1108
Size: 1536 216 4867
Fragments arranged by size:
4867 1536 216
PfoI T'CCnGG_A
Cuts at: 2052 2328 3846 2052
Size: 276 1518 4825
Fragments arranged by size:
4825 1518 276
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4688 4720 6201 6233 4688
Size: 32 1481 32 5074
Fragments arranged by size:
5074 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2974 2974
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PspOMI G'GGCC_C
Cuts at: 2254 2473 3878 2254
Size: 219 1405 4995
Fragments arranged by size:
4995 1405 219
PspXI vC'TCGA_Gb
Cuts at: 2570 2570
Size: 6619
PstI C_TGCA'G
Cuts at: 830 2238 2841 3006 3330 3573 3971 830
Size: 1408 603 165 324 243 398 3478
Fragments arranged by size:
3478 1408 603 398 324 243 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 2028 3154 3331 2028
Size: 1126 177 5316
Fragments arranged by size:
5316 1126 177
SacI G_AGCT'C
Cuts at: 3075 3075
Size: 6619
SacII CC_GC'GG
Cuts at: 2370 2503 2635 3535 2370
Size: 133 132 900 5454
Fragments arranged by size:
5454 900 133 132
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SapI GCTCTTCn'nnn_
Cuts at: 2370 3821 2370
Size: 1451 5168
SbfI CC_TGCA'GG
Cuts at: 2841 3006 3971 2841
Size: 165 965 5489
Fragments arranged by size:
5489 965 165
ScaI AGT'ACT
Cuts at: 4867 6579 4867
Size: 1712 4907
SfcI C'TryA_G
Cuts at: 826 1045 2234 2837 3002 3326 3569 3967 5102
Size: 219 1189 603 165 324 243 398 1135
Cuts at: 5102 5783 5974 826
Size: 681 191 1471
Fragments arranged by size:
1471 1189 1135 681 603 398 324 243
219 191 165
SfiI GGCCn_nnn'nGGCC
Cuts at: 3543 3543
Size: 6619
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 2260 3946 1872
Size: 388 1686 4545
Fragments arranged by size:
4545 1686 388
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2570 3227 3275 3830 3884 4723
Size: 197 926 627 657 48 555 54 839
Cuts at: 4723 5624 5886 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 839 657 627 555 262
197 54 48
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 4543 5
Size: 47 4491 2081
Fragments arranged by size:
4491 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 3734 1517
Size: 1095 1122 4402
Fragments arranged by size:
4402 1122 1095
StyI C'CwwG_G
Cuts at: 513 1065 2004 3678 4058 513
Size: 552 939 1674 380 3074
Fragments arranged by size:
3074 1674 939 552 380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2323 2720 3030 3416 3453 3836 4646
Size: 1255 743 397 310 386 37 383 810
Cuts at: 4646 4663 4816 5001 5446 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 810 743 445 397 386 383
310 185 153 37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4865 6577 96
Size: 274 80 33 51 1443 2888 1712 138
Fragments arranged by size:
2888 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 2531 2563 2582 2614 3026 3058 6579
Size: 32 292 32 19 32 412 32 3521
Cuts at: 6579 6611 2207
Size: 32 2215
Fragments arranged by size:
3521 2215 412 292 32 32 32 32
32 19
Tth111I GACn'n_nGTC
Cuts at: 1316 2990 1316
Size: 1674 4945
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2758 2758
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 2570 3227 1943
Size: 627 657 5335
Fragments arranged by size:
5335 657 627
XmaI C'CCGG_G
Cuts at: 1870 2258 3944 1870
Size: 388 1686 4545
Fragments arranged by size:
4545 1686 388
XmnI GAAnn'nnTTC
Cuts at: 4748 4748
Size: 6619
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PsrI RsrII
SanDI SexAI SfoI SrfI SwaI