Restriction map for
pFN21AE4175
(Circular) (Six-base) MAPSORT of: pFN21AE4175.seq Check: 5517 from: 1 to: 6610
pFN21AE4175 6610 bp
With 182 enzymes: SgfI *
March 28, 2012 13:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4420 278
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3953 1937
Size: 2016 4594
Acc65I G'GTAC_C
Cuts at: 3931 3931
Size: 6610
AclI AA'CG_TT
Cuts at: 1563 4737 5110 1563
Size: 3174 373 3063
Fragments arranged by size:
3174 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 3836 4673 6057 6325 1632
Size: 467 1737 837 1384 268 1917
Fragments arranged by size:
1917 1737 1384 837 467 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 2072 2364 3106 3327 3907 5509 6486 1203
Size: 869 292 742 221 580 1602 977 1327
Fragments arranged by size:
1602 1327 977 869 742 580 292 221
AgeI A'CCGG_T
Cuts at: 5441 5441
Size: 6610
AhdI GACnn_n'nnGTC
Cuts at: 5339 5339
Size: 6610
AleI CACnn'nnGTG
Cuts at: 1166 2807 1166
Size: 1641 4969
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2180 3784 1184
Size: 996 1604 4010
Fragments arranged by size:
4010 1604 996
AlwNI CAG_nnn'CTG
Cuts at: 2569 3466 4353 5925 2569
Size: 897 887 1572 3254
Fragments arranged by size:
3254 1572 897 887
ApaI G_GGCC'C
Cuts at: 2523 2576 3724 2523
Size: 53 1148 5409
Fragments arranged by size:
5409 1148 53
ApaLI G'TGCA_C
Cuts at: 2810 3372 4667 5823 2810
Size: 562 1295 1156 3597
Fragments arranged by size:
3597 1295 1156 562
ApoI r'AATT_y
Cuts at: 1493 3919 4145 4297 6406 1493
Size: 2426 226 152 2109 1697
Fragments arranged by size:
2426 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2302 2670 3240 3549 3585 3707
Size: 73 282 77 368 570 309 36 122
Cuts at: 3707 3741 3935 1870
Size: 34 194 4545
Fragments arranged by size:
4545 570 368 309 282 194 122 77
73 36 34
BamHI G'GATC_C
Cuts at: 2656 3940 4307 2656
Size: 1284 367 4959
Fragments arranged by size:
4959 1284 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 2600 3406 3931 5386
Size: 325 217 627 511 302 806 525 1455
Cuts at: 5386 618
Size: 1842
Fragments arranged by size:
1842 1455 806 627 525 511 325 302
217
BanII G_rGCy'C
Cuts at: 1391 2394 2495 2523 2576 2841 3724 3929 1391
Size: 1003 101 28 53 265 883 205 4072
Fragments arranged by size:
4072 1003 883 265 205 101 53 28
BbeI G_GCGC'C
Cuts at: 2604 2604
Size: 6610
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 928
Size: 971 403 5236
Fragments arranged by size:
5236 971 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4801 4835 1903
Size: 34 2864 34 3678
Fragments arranged by size:
3678 2864 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4502 5712 4502
Size: 1210 5400
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 2471 5221
Size: 107 122 71 631 605 526 273 2750
Cuts at: 5221 136
Size: 1525
Fragments arranged by size:
2750 1525 631 605 526 273 122 107
71
BglII A'GATC_T
Cuts at: 6605 6605
Size: 6610
BlpI GC'TnA_GC
Cuts at: 2996 3210 3779 4027 2996
Size: 214 569 248 5579
Fragments arranged by size:
5579 569 248 214
Bme1580I G_kGCm'C
Cuts at: 1790 2523 2576 2814 3367 3376 3724 4671 5827
Size: 733 53 238 553 9 348 947 1156
Cuts at: 5827 1790
Size: 2573
Fragments arranged by size:
2573 1156 947 733 553 348 238 53
9
BmgBI CAC'GTC
Cuts at: 1318 2365 2422 3028 3342 1318
Size: 1047 57 606 314 4586
Fragments arranged by size:
4586 1047 606 314 57
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2579 3323 3795
Size: 416 53 83 399 24 1162 744 472
Cuts at: 3795 4341 5299 442
Size: 546 958 1753
Fragments arranged by size:
1753 1162 958 744 546 472 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6610
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3692
Size: 6610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2759 2906 3005 3530 5270 1239
Size: 1520 147 99 525 1740 2579
Fragments arranged by size:
2579 1740 1520 525 147 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4735 5600 5898 4735
Size: 865 298 5447
Fragments arranged by size:
5447 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2141 2222 2408 2722 2735 5273 882
Size: 617 642 81 186 314 13 2538 2219
Fragments arranged by size:
2538 2219 642 617 314 186 81 13
BsaAI yAC'GTr
Cuts at: 493 1204 2320 3490 4401 493
Size: 711 1116 1170 911 2702
Fragments arranged by size:
2702 1170 1116 911 711
BsaBI GATnn'nnATC
Cuts at: 2120 6604 2120
Size: 4484 2126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2105 2307 2463 2601
Size: 53 83 186 936 572 202 156 138
Cuts at: 2601 4417 4799 5434 275
Size: 1816 382 635 1451
Fragments arranged by size:
1816 1451 936 635 572 382 202 186
156 138 83 53
BsaWI w'CCGG_w
Cuts at: 3816 5043 5441 5715 5862 6273 3816
Size: 1227 398 274 147 411 4153
Fragments arranged by size:
4153 1227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6385
Fragments arranged by size:
6385 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2099 2402 2483 2656 3551 3677 3685
Size: 831 82 303 81 173 895 126 8
Cuts at: 3685 3725 1186
Size: 40 4071
Fragments arranged by size:
4071 895 831 303 173 126 82 81
40 8
BseYI C'CCAG_C
Cuts at: 2522 3031 3112 3887 5813 6309 2522
Size: 509 81 775 1926 496 2823
Fragments arranged by size:
2823 1926 775 509 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 1163
Size: 573 597 5440
Fragments arranged by size:
5440 597 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2767 3500 4060 4821 5849 1540
Size: 275 186 766 733 560 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 733 560 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2394 2814 3183 3376 3390 4671 4756 5827
Size: 69 420 369 193 14 1281 85 1071
Cuts at: 5827 2325
Size: 3108
Fragments arranged by size:
3108 1281 1071 420 369 193 85 69
14
BsmI GAATG_Cn'
Cuts at: 3856 4121 4214 3856
Size: 265 93 6252
Fragments arranged by size:
6252 265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 2394 2495 2523 2576 2814 2841
Size: 399 535 69 101 28 53 238 27
Cuts at: 2841 3183 3367 3376 3390 3724 3929 4671 4756
Size: 342 184 9 14 334 205 742 85
Cuts at: 4756 5827 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 535 399 342 334 238
205 184 101 85 69 53 28 27
14 9
BspEI T'CCGG_A
Cuts at: 3816 6273 3816
Size: 2457 4153
BspHI T'CATG_A
Cuts at: 4499 6229 4499
Size: 1730 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2949 3965 844
Size: 2105 1016 3489
Fragments arranged by size:
3489 2105 1016
BsrBI CCG'CTC
Cuts at: 1394 1823 2377 2548 3000 4497 1394
Size: 429 554 171 452 1497 3507
Fragments arranged by size:
3507 1497 554 452 429 171
BsrDI GCAATG_nn'
Cuts at: 65 1248 5105 5279 65
Size: 1183 3857 174 1396
Fragments arranged by size:
3857 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2220 2339 2448 2534 3403 3499 5254
Size: 110 410 119 109 86 869 96 1755
Cuts at: 5254 5441 1700
Size: 187 2869
Fragments arranged by size:
2869 1755 869 410 187 119 110 109
96 86
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6610
BssSI C'ACGA_G
Cuts at: 2579 4670 5682 2579
Size: 2091 1012 3507
Fragments arranged by size:
3507 2091 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2907 3371 3466 794
Size: 2113 464 95 3938
Fragments arranged by size:
3938 2113 464 95
BstBI TT'CG_AA
Cuts at: 2382 5414 2382
Size: 3032 3578
BstEII G'GTnAC_C
Cuts at: 1184 3298 1184
Size: 2114 4496
BstYI r'GATC_y
Cuts at: 1972 2656 2781 3940 4307 4692 4709 6161 6247
Size: 684 125 1159 367 385 17 1452 86
Cuts at: 6247 6259 6605 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1159 684 385 367 346 125
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3125 3864 3125
Size: 739 5871
BtgI C'CryG_G
Cuts at: 513 1065 2035 2358 2596 2620 3450 3494 513
Size: 552 970 323 238 24 830 44 3629
Fragments arranged by size:
3629 970 830 552 323 238 44 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3379 3651 487
Size: 683 56 2153 272 3446
Fragments arranged by size:
3446 2153 683 272 56
BtsI GCAGTG_nn'
Cuts at: 2350 3654 4126 4920 4940 2350
Size: 1304 472 794 20 4020
Fragments arranged by size:
4020 1304 794 472 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6575
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DraIII CAC_nnn'GTG
Cuts at: 3097 3371 3097
Size: 274 6336
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5617 809
Size: 507 4301 1802
Fragments arranged by size:
4301 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2199 2964
Size: 54 1425 50 275 18 261 108 765
Cuts at: 2964 3447 3453 3497 4057 4946 6575 8
Size: 483 6 44 560 889 1629 43
Fragments arranged by size:
1629 1425 889 765 560 483 275 261
108 54 50 44 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3497 4057 1537
Size: 275 1685 560 4090
Fragments arranged by size:
4090 1685 560 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2384 2654 2708 3585 4540 1353
Size: 1026 5 270 54 877 955 3423
Fragments arranged by size:
3423 1026 955 877 270 54 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5197 5571 5717 387
Size: 1064 138 3608 374 146 1280
Fragments arranged by size:
3608 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 2392
Size: 6610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2759 2906 3005 3530 3836 4673
Size: 393 467 660 147 99 525 306 837
Cuts at: 4673 5270 6057 6325 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 837 787 660 597 525 467 393
306 268 147 99
EcoO109I rG'GnC_Cy
Cuts at: 2519 2573 3228 3721 2519
Size: 54 655 493 5408
Fragments arranged by size:
5408 655 493 54
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FseI GG_CCGG'CC
Cuts at: 2224 2224
Size: 6610
FspI TGC'GCA
Cuts at: 2168 2900 3062 5116 2168
Size: 732 162 2054 3662
Fragments arranged by size:
3662 2054 732 162
FspAI rTGC'GCAy
Cuts at: 2900 3062 2900
Size: 162 6448
HaeII r_GCGC'y
Cuts at: 1678 2477 2604 2648 3718 5757 1678
Size: 799 127 44 1070 2039 2531
Fragments arranged by size:
2531 2039 1070 799 127 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 5251 5283 5325
Size: 32 135 32 1076 32 2571 32 42
Cuts at: 5325 5357 1373
Size: 32 2626
Fragments arranged by size:
2626 2571 1076 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3421 3954 4200 669
Size: 988 281 1483 533 246 3079
Fragments arranged by size:
3079 1483 988 533 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6610
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KasI G'GCGC_C
Cuts at: 2600 2600
Size: 6610
KpnI G_GTAC'C
Cuts at: 3935 3935
Size: 6610
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 3106 6486 3106
Size: 3380 3230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 3258 3905 5724 5908 1305
Size: 783 1170 647 1819 184 2007
Fragments arranged by size:
2007 1819 1170 783 647 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 2966 3449 3455 10
Size: 54 1425 343 261 873 483 6 3165
Fragments arranged by size:
3165 1425 873 483 343 261 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2807 3398 3404 4568 4927 5086
Size: 648 960 681 591 6 1164 359 159
Cuts at: 5086 518
Size: 2042
Fragments arranged by size:
2042 1164 960 681 648 591 359 159
6
MspA1I CmG'CkG
Cuts at: 1606 2037 2256 2265 2360 2511 2622 3508 3764
Size: 431 219 9 95 151 111 886 256
Cuts at: 3764 4026 4703 5851 6096 1606
Size: 262 677 1148 245 2120
Fragments arranged by size:
2120 1148 886 677 431 262 256 245
219 151 111 95 9
NaeI GCC'GGC
Cuts at: 2222 2536 3501 2222
Size: 314 965 5331
Fragments arranged by size:
5331 965 314
NarI GG'CG_CC
Cuts at: 2601 2601
Size: 6610
NcoI C'CATG_G
Cuts at: 513 1065 2596 3450 513
Size: 552 1531 854 3673
Fragments arranged by size:
3673 1531 854 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6610
NgoMIV G'CCGG_C
Cuts at: 2220 2534 3499 2220
Size: 314 965 5331
Fragments arranged by size:
5331 965 314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6610
NotI GC'GGCC_GC
Cuts at: 4057 4057
Size: 6610
NspI r_CATG'y
Cuts at: 1132 1229 2076 2641 3331 3355 3436 3876 3911
Size: 97 847 565 690 24 81 440 35
Cuts at: 3911 3968 5513 1132
Size: 57 1545 2229
Fragments arranged by size:
2229 1545 847 690 565 440 97 81
57 35 24
PasI CC'CwG_GG
Cuts at: 1348 3172 1348
Size: 1824 4786
PciI A'CATG_T
Cuts at: 2072 3327 3907 5509 2072
Size: 1255 580 1602 3173
Fragments arranged by size:
3173 1602 1255 580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3097 3342 3688 3887 1108
Size: 1989 245 346 199 3831
Fragments arranged by size:
3831 1989 346 245 199
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PmlI CAC'GTG
Cuts at: 3490 3490
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4679 4711 6192 6224 4679
Size: 32 1481 32 5065
Fragments arranged by size:
5065 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PspOMI G'GGCC_C
Cuts at: 2519 2572 3720 2519
Size: 53 1148 5409
Fragments arranged by size:
5409 1148 53
PstI C_TGCA'G
Cuts at: 830 2565 3962 830
Size: 1735 1397 3478
Fragments arranged by size:
3478 1735 1397
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 3508 3764 3508
Size: 256 6354
SacI G_AGCT'C
Cuts at: 2394 2394
Size: 6610
SacII CC_GC'GG
Cuts at: 2038 2361 2623 2038
Size: 323 262 6025
Fragments arranged by size:
6025 323 262
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SapI GCTCTTCn'nnn_
Cuts at: 2384 2654 2384
Size: 270 6340
SbfI CC_TGCA'GG
Cuts at: 3962 3962
Size: 6610
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SfcI C'TryA_G
Cuts at: 826 1045 2561 3562 3958 5093 5774 5965 826
Size: 219 1516 1001 396 1135 681 191 1471
Fragments arranged by size:
1516 1471 1135 1001 681 396 219 191
SfoI GGC'GCC
Cuts at: 2602 2602
Size: 6610
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 2304 2672 3551 3587 3937 1872
Size: 432 368 879 36 350 4545
Fragments arranged by size:
4545 879 432 368 350 36
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3741 4714 5615 5877 820
Size: 197 926 1798 973 901 262 1553
Fragments arranged by size:
1798 1553 973 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4401 493
Size: 3908 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 3355 3876 3968 1132
Size: 2223 521 92 3774
Fragments arranged by size:
3774 2223 521 92
SspI AAT'ATT
Cuts at: 5 52 4534 5
Size: 47 4482 2081
Fragments arranged by size:
4482 2081 47
StuI AGG'CCT
Cuts at: 1517 2753 1517
Size: 1236 5374
StyI C'CwwG_G
Cuts at: 513 1065 2094 2596 3012 3450 4049 513
Size: 552 1029 502 416 438 599 3074
Fragments arranged by size:
3074 1029 599 552 502 438 416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 2432 3164 3532 3695 4637 4654
Size: 1255 666 186 732 368 163 942 17
Cuts at: 4654 4807 4992 5437 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 942 732 666 445 368 186
185 163 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3435 3522 4856
Size: 274 80 33 51 1443 1458 87 1334
Cuts at: 4856 6568 96
Size: 1712 138
Fragments arranged by size:
1712 1458 1443 1334 274 138 87 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2874 2906 6570 6602 2874
Size: 32 3664 32 2882
Fragments arranged by size:
3664 2882 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2930 3343 1316
Size: 1614 413 4583
Fragments arranged by size:
4583 1614 413
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2734 3310 2734
Size: 576 6034
XhoI C'TCGA_G
Cuts at: 1943 3741 1943
Size: 1798 4812
XmaI C'CCGG_G
Cuts at: 1870 2302 2670 3549 3585 3935 1870
Size: 432 368 879 36 350 4545
Fragments arranged by size:
4545 879 432 368 350 36
XmnI GAAnn'nnTTC
Cuts at: 4739 4739
Size: 6610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4418 276
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI
NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII
SanDI SexAI SfiI SrfI SwaI