Restriction map for
pFN21AE4173
(Circular) (Six-base) MAPSORT of: pFN21AE4173.seq Check: 870 from: 1 to: 6607
pFN21AE4173 6607 bp
With 182 enzymes: SgfI *
March 28, 2012 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 3312 2189
Size: 1123 5484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3405 4417 278
Size: 53 83 186 936 1869 1012 2468
Fragments arranged by size:
2468 1869 1012 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3691 3950 1937
Size: 1754 259 4594
Fragments arranged by size:
4594 1754 259
Acc65I G'GTAC_C
Cuts at: 3928 3928
Size: 6607
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 4670 6054 6322 1632
Size: 599 2439 1384 268 1917
Fragments arranged by size:
2439 1917 1384 599 268
AfeI AGC'GCT
Cuts at: 2781 2781
Size: 6607
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 2355 5506 6483 1203
Size: 1152 3151 977 1327
Fragments arranged by size:
3151 1327 1152 977
AgeI A'CCGG_T
Cuts at: 3213 5438 3213
Size: 2225 4382
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 3230 1166
Size: 2064 4543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3248 3289 3591 1184
Size: 2064 41 302 4200
Fragments arranged by size:
4200 2064 302 41
AlwNI CAG_nnn'CTG
Cuts at: 2257 3095 3346 4350 5922 2257
Size: 838 251 1004 1572 2942
Fragments arranged by size:
2942 1572 1004 838 251
ApaI G_GGCC'C
Cuts at: 2028 2039 3153 2028
Size: 11 1114 5482
Fragments arranged by size:
5482 1114 11
ApaLI G'TGCA_C
Cuts at: 2470 4664 5820 2470
Size: 2194 1156 3257
Fragments arranged by size:
3257 2194 1156
ApoI r'AATT_y
Cuts at: 1493 3038 3916 4142 4294 6403 1493
Size: 1545 878 226 152 2109 1697
Fragments arranged by size:
2109 1697 1545 878 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2056 3932 1870
Size: 73 95 18 1876 4545
Fragments arranged by size:
4545 1876 95 73 18
BamHI G'GATC_C
Cuts at: 3937 4304 3937
Size: 367 6240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3300 3664 3928 5383 618
Size: 325 217 627 1513 364 264 1455 1842
Fragments arranged by size:
1842 1513 1455 627 364 325 264 217
BanII G_rGCy'C
Cuts at: 1391 2028 2039 2045 2229 2490 3153 3654 3805
Size: 637 11 6 184 261 663 501 151
Cuts at: 3805 3926 1391
Size: 121 4072
Fragments arranged by size:
4072 663 637 501 261 184 151 121
11 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2105 3469 3779 928
Size: 971 206 1364 310 3756
Fragments arranged by size:
3756 1364 971 310 206
BbvCI CC'TCA_GC
Cuts at: 2906 3563 3730 2906
Size: 657 167 5783
Fragments arranged by size:
5783 657 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2120 2154 3454 3488 4798 4832 1903
Size: 34 183 34 1300 34 1310 34 3678
Fragments arranged by size:
3678 1310 1300 183 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4499 5709 4499
Size: 1210 5397
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6607
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 2639 2921
Size: 107 122 71 631 605 482 485 282
Cuts at: 2921 5218 136
Size: 2297 1525
Fragments arranged by size:
2297 1525 631 605 485 482 282 122
107 71
BglII A'GATC_T
Cuts at: 6602 6602
Size: 6607
BlpI GC'TnA_GC
Cuts at: 4024 4024
Size: 6607
Bme1580I G_kGCm'C
Cuts at: 1790 2028 2039 2081 2463 2474 2569 3153 4668
Size: 238 11 42 382 11 95 584 1515
Cuts at: 4668 5824 1790
Size: 1156 2573
Fragments arranged by size:
2573 1515 1156 584 382 238 95 42
11 11
BmgBI CAC'GTC
Cuts at: 1318 2332 2458 3080 3430 1318
Size: 1014 126 622 350 4495
Fragments arranged by size:
4495 1014 622 350 126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3161 4338 5296
Size: 416 53 83 399 24 1744 1177 958
Cuts at: 5296 442
Size: 1753
Fragments arranged by size:
1753 1744 1177 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 3469 2860
Size: 609 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2333 2636 3074 3542 3668 5267 1239
Size: 812 282 303 438 468 126 1599 2579
Fragments arranged by size:
2579 1599 812 468 438 303 282 126
Bpu10I CC'TnA_GC
Cuts at: 2269 2906 3127 3563 3730 2269
Size: 637 221 436 167 5146
Fragments arranged by size:
5146 637 436 221 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4732 5597 5895 4732
Size: 865 298 5444
Fragments arranged by size:
5444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 3348 5270 882
Size: 617 831 1018 1922 2219
Fragments arranged by size:
2219 1922 1018 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 2092 2343 2358 2596 4398 493
Size: 711 888 251 15 238 1802 2702
Fragments arranged by size:
2702 1802 888 711 251 238 15
BsaBI GATnn'nnATC
Cuts at: 6601 6601
Size: 6607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3402 4414 4796 5431
Size: 53 83 186 936 1869 1012 382 635
Cuts at: 5431 275
Size: 1451
Fragments arranged by size:
1869 1451 1012 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3213 5040 5438 5712 5859 6270 3213
Size: 1827 398 274 147 411 3550
Fragments arranged by size:
3550 1827 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 3146 3176 3605
Size: 30 165 30 924 30 789 30 429
Cuts at: 3605 3635 3641 3671 1178
Size: 30 6 30 4114
Fragments arranged by size:
4114 924 789 429 165 30 30 30
30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 2732 2735 2887 1186
Size: 1522 24 3 152 4906
Fragments arranged by size:
4906 1522 152 24 3
BseYI C'CCAG_C
Cuts at: 2219 3070 3540 3798 5810 6306 2219
Size: 851 470 258 2012 496 2520
Fragments arranged by size:
2520 2012 851 496 470 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 2338 2462 2618 2819 3194 3332
Size: 573 378 224 124 156 201 375 138
Cuts at: 3332 3392 3452 1163
Size: 60 60 4318
Fragments arranged by size:
4318 573 378 375 224 201 156 138
124 60 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2166 2173 2984 4057 4818 5846
Size: 275 186 165 7 811 1073 761 1028
Cuts at: 5846 1540
Size: 2301
Fragments arranged by size:
2301 1073 1028 811 761 275 186 165
7
BsiHKAI G_wGCw'C
Cuts at: 2045 2407 2474 3273 3450 4668 4753 5824 2045
Size: 362 67 799 177 1218 85 1071 2828
Fragments arranged by size:
2828 1218 1071 799 362 177 85 67
BsmI GAATG_Cn'
Cuts at: 3580 4118 4211 3580
Size: 538 93 5976
Fragments arranged by size:
5976 538 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2387 3393 2387
Size: 1006 5601
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2039 2045 2081 2229 2407 2463
Size: 399 238 11 6 36 148 178 56
Cuts at: 2463 2474 2490 2569 3153 3273 3450 3654 3805
Size: 11 16 79 584 120 177 204 151
Cuts at: 3805 3926 4668 4753 5824 1391
Size: 121 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 584 399 238 204 178
177 151 148 121 120 85 79 56
36 16 11 11 6
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 4496 6226 4496
Size: 1730 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 2866 3312 3962 844
Size: 1345 677 446 650 3489
Fragments arranged by size:
3489 1345 677 650 446
BsrBI CCG'CTC
Cuts at: 1394 1823 2611 3621 3643 4494 1394
Size: 429 788 1010 22 851 3507
Fragments arranged by size:
3507 1010 851 788 429 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 3624 5102 5276 65
Size: 1183 2376 1478 174 1396
Fragments arranged by size:
2376 1478 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 2233 2241 3213 3297 5251 5438
Size: 110 331 92 8 972 84 1954 187
Cuts at: 5438 1700
Size: 2869
Fragments arranged by size:
2869 1954 972 331 187 110 92 84
8
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 2376 2378 2417 3044 1925
Size: 451 2 39 627 5488
Fragments arranged by size:
5488 627 451 39 2
BssSI C'ACGA_G
Cuts at: 4667 5679 4667
Size: 1012 5595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2358 2535 2626 2797 3095 3104 794
Size: 1564 177 91 171 298 9 4297
Fragments arranged by size:
4297 1564 298 177 171 91 9
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 3186 1184
Size: 2002 4605
BstYI r'GATC_y
Cuts at: 1972 3854 3937 4304 4689 4706 6158 6244 6256
Size: 1882 83 367 385 17 1452 86 12
Cuts at: 6256 6602 1972
Size: 346 1977
Fragments arranged by size:
1977 1882 1452 385 367 346 86 83
17 12
Bsu36I CC'TnA_GG
Cuts at: 2993 2993
Size: 6607
BtgI C'CryG_G
Cuts at: 513 1065 2685 3669 513
Size: 552 1620 984 3451
Fragments arranged by size:
3451 1620 984 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2653 3891 487
Size: 683 56 1427 1238 3203
Fragments arranged by size:
3203 1427 1238 683 56
BtsI GCAGTG_nn'
Cuts at: 2569 3059 3154 3271 4123 4917 4937 2569
Size: 490 95 117 852 794 20 4239
Fragments arranged by size:
4239 852 794 490 117 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DraIII CAC_nnn'GTG
Cuts at: 2575 2575
Size: 6607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5614 809
Size: 507 4298 1802
Fragments arranged by size:
4298 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2170 2631
Size: 54 1425 50 275 18 325 15 461
Cuts at: 2631 2652 2853 2913 2981 4054 4943 6572 8
Size: 21 201 60 68 1073 889 1629 43
Fragments arranged by size:
1629 1425 1073 889 461 325 275 201
68 60 54 50 43 21 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2170 2981 4054 1537
Size: 275 358 811 1073 4090
Fragments arranged by size:
4090 1073 811 358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2747 2808 3816 4537 1353
Size: 1394 61 1008 721 3423
Fragments arranged by size:
3423 1394 1008 721 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3257 3401 5194 5568 5714 387
Size: 1064 138 1668 144 1793 374 146 1280
Fragments arranged by size:
1793 1668 1280 1064 374 146 144 138
EcoICRI GAG'CTC
Cuts at: 2043 2043
Size: 6607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2231 2333 2636 3074 3542 3668
Size: 393 419 180 102 303 438 468 126
Cuts at: 3668 4670 5267 6054 6322 1239
Size: 1002 597 787 268 1524
Fragments arranged by size:
1524 1002 787 597 468 438 419 393
303 268 180 126 102
EcoNI CCTnn'n_nnAGG
Cuts at: 3733 3733
Size: 6607
EcoO109I rG'GnC_Cy
Cuts at: 2025 2035 2216 2641 3131 3149 3150 2025
Size: 10 181 425 490 18 1 5482
Fragments arranged by size:
5482 490 425 181 18 10 1
EcoRI G'AATT_C
Cuts at: 3038 3916 4294 3038
Size: 878 378 5351
Fragments arranged by size:
5351 878 378
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 2107 2712 2783 3143 5754 1678
Size: 429 605 71 360 2611 2531
Fragments arranged by size:
2611 2531 605 429 360 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2570 2602 3665 3697 3761
Size: 32 135 32 998 32 1063 32 64
Cuts at: 3761 3793 5248 5280 5322 5354 1373
Size: 32 1455 32 42 32 2626
Fragments arranged by size:
2626 1455 1063 998 135 64 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3951 4197 669
Size: 988 281 2013 246 3079
Fragments arranged by size:
3079 2013 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KpnI G_GTAC'C
Cuts at: 3932 3932
Size: 6607
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5721 5905 1305
Size: 4416 184 2007
Fragments arranged by size:
4416 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2855 2915 10
Size: 54 1425 343 1023 60 3702
Fragments arranged by size:
3702 1425 1023 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3230 4565 4924 5083 518
Size: 648 2064 1335 359 159 2042
Fragments arranged by size:
2064 2042 1335 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2083 2188 2223 2535 3092 3562 3798
Size: 465 12 105 35 312 557 470 236
Cuts at: 3798 4023 4700 5848 6093 1606
Size: 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 557 470 465 312 245
236 225 105 35 12
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 6607
NcoI C'CATG_G
Cuts at: 513 1065 2685 513
Size: 552 1620 4435
Fragments arranged by size:
4435 1620 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 6607
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 2981 4054 2981
Size: 1073 5534
NspI r_CATG'y
Cuts at: 1132 1229 2413 2551 2755 3965 5510 1132
Size: 97 1184 138 204 1210 1545 2229
Fragments arranged by size:
2229 1545 1210 1184 204 138 97
PasI CC'CwG_GG
Cuts at: 1348 2437 1348
Size: 1089 5518
PciI A'CATG_T
Cuts at: 5506 5506
Size: 6607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2071 1108
Size: 963 5644
PfoI T'CCnGG_A
Cuts at: 2931 3480 3745 3756 2931
Size: 549 265 11 5782
Fragments arranged by size:
5782 549 265 11
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PmlI CAC'GTG
Cuts at: 2092 2358 2092
Size: 266 6341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 4676 4708 6189 6221 3800
Size: 32 844 32 1481 32 4186
Fragments arranged by size:
4186 1481 844 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2216 2216
Size: 6607
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PspOMI G'GGCC_C
Cuts at: 2024 2035 3149 2024
Size: 11 1114 5482
Fragments arranged by size:
5482 1114 11
PstI C_TGCA'G
Cuts at: 830 2253 2649 2682 2793 2877 2967 3309 3959
Size: 1423 396 33 111 84 90 342 650
Cuts at: 3959 830
Size: 3478
Fragments arranged by size:
3478 1423 650 396 342 111 90 84
33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 3092 3562 3798 3092
Size: 470 236 5901
Fragments arranged by size:
5901 470 236
SacI G_AGCT'C
Cuts at: 2045 2045
Size: 6607
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SanDI GG'GwC_CC
Cuts at: 2216 2216
Size: 6607
SapI GCTCTTCn'nnn_
Cuts at: 3816 3816
Size: 6607
SbfI CC_TGCA'GG
Cuts at: 2877 3309 3959 2877
Size: 432 650 5525
Fragments arranged by size:
5525 650 432
ScaI AGT'ACT
Cuts at: 4855 6567 4855
Size: 1712 4895
SexAI A'CCwGG_T
Cuts at: 2883 3333 2883
Size: 450 6157
SfcI C'TryA_G
Cuts at: 826 1045 2249 2645 2678 2789 2873 2963 3305
Size: 219 1204 396 33 111 84 90 342
Cuts at: 3305 3692 3955 5090 5771 5962 826
Size: 387 263 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 396 387 342 263
219 191 111 90 84 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 2639 2639
Size: 6607
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 2233 1700
Size: 533 6074
SmaI CCC'GGG
Cuts at: 1872 3934 1872
Size: 2062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4711 5612 5874 820
Size: 197 926 2768 901 262 1553
Fragments arranged by size:
2768 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 2755 3965 1132
Size: 1623 1210 3774
Fragments arranged by size:
3774 1623 1210
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 2828 2998 1517
Size: 1311 170 5126
Fragments arranged by size:
5126 1311 170
StyI C'CwwG_G
Cuts at: 513 1065 2685 3135 3228 3630 4046 513
Size: 552 1620 450 93 402 416 3074
Fragments arranged by size:
3074 1620 552 450 416 402 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 4634 4651 4804 4989 5434 325
Size: 1255 600 2454 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 600 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4853 6565 96
Size: 274 80 33 51 1443 2876 1712 138
Fragments arranged by size:
2876 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 6567 6599 3146
Size: 32 3389 32 3154
Fragments arranged by size:
3389 3154 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 3235 2308
Size: 927 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3932 1870
Size: 2062 4545
XmnI GAAnn'nnTTC
Cuts at: 4736 4736
Size: 6607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3403 4415 276
Size: 53 83 186 936 1869 1012 2468
Fragments arranged by size:
2468 1869 1012 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SacII SfoI SrfI
SwaI