Restriction map for
pFN21AE4172
(Circular) (Six-base) MAPSORT of: pFN21AE4172.seq Check: 4225 from: 1 to: 6604
pFN21AE4172 6604 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4414 278
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3037 3947 1937
Size: 1100 910 4594
Fragments arranged by size:
4594 1100 910
Acc65I G'GTAC_C
Cuts at: 3925 3925
Size: 6604
AclI AA'CG_TT
Cuts at: 1563 4731 5104 1563
Size: 3168 373 3063
Fragments arranged by size:
3168 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4667 6051 6319 1632
Size: 3035 1384 268 1917
Fragments arranged by size:
3035 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6407
AflIII A'CryG_T
Cuts at: 1203 2389 2814 5503 6480 1203
Size: 1186 425 2689 977 1327
Fragments arranged by size:
2689 1327 1186 977 425
AgeI A'CCGG_T
Cuts at: 5435 5435
Size: 6604
AhdI GACnn_n'nnGTC
Cuts at: 5333 5333
Size: 6604
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6604
AlwNI CAG_nnn'CTG
Cuts at: 2083 4347 5919 2083
Size: 2264 1572 2768
Fragments arranged by size:
2768 2264 1572
ApaI G_GGCC'C
Cuts at: 2120 2120
Size: 6604
ApaLI G'TGCA_C
Cuts at: 4661 5817 4661
Size: 1156 5448
ApoI r'AATT_y
Cuts at: 1493 2261 2633 3193 3321 3439 3611 3913 4139
Size: 768 372 560 128 118 172 302 226
Cuts at: 4139 4291 6400 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 768 560 372 302 226 172
152 128 118
AseI AT'TA_AT
Cuts at: 160 1032 5158 160
Size: 872 4126 1606
Fragments arranged by size:
4126 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
AvaI C'yCGr_G
Cuts at: 1870 1943 3929 1870
Size: 73 1986 4545
Fragments arranged by size:
4545 1986 73
BamHI G'GATC_C
Cuts at: 3934 4301 3934
Size: 367 6237
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2067 3925 5380 618
Size: 325 217 627 280 1858 1455 1842
Fragments arranged by size:
1858 1842 1455 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2120 3923 1391
Size: 729 1803 4072
Fragments arranged by size:
4072 1803 729
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2047 3680 928
Size: 971 148 1633 3852
Fragments arranged by size:
3852 1633 971 148
BbvCI CC'TCA_GC
Cuts at: 2135 2135
Size: 6604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4795 4829 1903
Size: 34 50 34 2774 34 3678
Fragments arranged by size:
3678 2774 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2334 4496 5706 2334
Size: 2162 1210 3232
Fragments arranged by size:
3232 2162 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6604
BfrBI ATG'CAT
Cuts at: 2532 2760 3600 2532
Size: 228 840 5536
Fragments arranged by size:
5536 840 228
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 5215 136
Size: 107 122 71 631 605 452 3091 1525
Fragments arranged by size:
3091 1525 631 605 452 122 107 71
BglII A'GATC_T
Cuts at: 2538 3271 6599 2538
Size: 733 3328 2543
Fragments arranged by size:
3328 2543 733
BlpI GC'TnA_GC
Cuts at: 2060 4021 2060
Size: 1961 4643
Bme1580I G_kGCm'C
Cuts at: 1790 2120 4665 5821 1790
Size: 330 2545 1156 2573
Fragments arranged by size:
2573 2545 1156 330
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 4335 5293
Size: 416 53 83 399 24 892 2026 958
Cuts at: 5293 442
Size: 1753
Fragments arranged by size:
2026 1753 958 892 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3006 5264 1239
Size: 1767 2258 2579
Fragments arranged by size:
2579 2258 1767
Bpu10I CC'TnA_GC
Cuts at: 2135 2135
Size: 6604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 4729 5594 5892 2068
Size: 2661 865 298 2780
Fragments arranged by size:
2780 2661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5267 882
Size: 617 3768 2219
Fragments arranged by size:
3768 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4395 493
Size: 711 3191 2702
Fragments arranged by size:
3191 2702 711
BsaBI GATnn'nnATC
Cuts at: 3735 6598 3735
Size: 2863 3741
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4411 4793 5428 275
Size: 53 83 186 936 2878 382 635 1451
Fragments arranged by size:
2878 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5037 5435 5709 5856 6267 5037
Size: 398 274 147 411 5374
Fragments arranged by size:
5374 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2970 3000 1178
Size: 30 165 30 1567 30 4782
Fragments arranged by size:
4782 1567 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2159 1186
Size: 970 3 5631
Fragments arranged by size:
5631 970 3
BseYI C'CCAG_C
Cuts at: 2244 5807 6303 2244
Size: 3563 496 2545
Fragments arranged by size:
3563 2545 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2614 1163
Size: 573 878 5153
Fragments arranged by size:
5153 878 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4054 4815 5843 1540
Size: 275 186 2053 761 1028 2301
Fragments arranged by size:
2301 2053 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3758 4665 4750 5821 3758
Size: 907 85 1071 4541
Fragments arranged by size:
4541 1071 907 85
BsmI GAATG_Cn'
Cuts at: 2762 4115 4208 2762
Size: 1353 93 5158
Fragments arranged by size:
5158 1353 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 3758 3923 4665 4750 5821 1391
Size: 399 330 1638 165 742 85 1071 2174
Fragments arranged by size:
2174 1638 1071 742 399 330 165 85
BspEI T'CCGG_A
Cuts at: 6267 6267
Size: 6604
BspHI T'CATG_A
Cuts at: 4493 6223 4493
Size: 1730 4874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 3959 844
Size: 1643 1472 3489
Fragments arranged by size:
3489 1643 1472
BsrBI CCG'CTC
Cuts at: 1394 1823 4491 1394
Size: 429 2668 3507
Fragments arranged by size:
3507 2668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3591 5099 5273 65
Size: 1183 2343 1508 174 1396
Fragments arranged by size:
2343 1508 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5248 5435 1700
Size: 110 3438 187 2869
Fragments arranged by size:
3438 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6604
BssSI C'ACGA_G
Cuts at: 4664 5676 4664
Size: 1012 5592
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3147 794
Size: 2353 4251
BstBI TT'CG_AA
Cuts at: 5408 5408
Size: 6604
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6604
BstXI CCAn_nnnn'nTGG
Cuts at: 3586 3586
Size: 6604
BstYI r'GATC_y
Cuts at: 1972 2538 3271 3934 4301 4686 4703 6155 6241
Size: 566 733 663 367 385 17 1452 86
Cuts at: 6241 6253 6599 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 733 663 566 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5865
Fragments arranged by size:
5865 683 56
BtsI GCAGTG_nn'
Cuts at: 2978 3207 3539 3579 4120 4914 4934 2978
Size: 229 332 40 541 794 20 4648
Fragments arranged by size:
4648 794 541 332 229 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6604
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6569
DraI TTT'AAA
Cuts at: 3908 3908
Size: 6604
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5611 809
Size: 507 4295 1802
Fragments arranged by size:
4295 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4051 4940 6569
Size: 54 1425 50 275 18 2221 889 1629
Cuts at: 6569 8
Size: 43
Fragments arranged by size:
2221 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4051 1537
Size: 275 2239 4090
Fragments arranged by size:
4090 2239 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4534 1353
Size: 3181 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5191 5565 5711 387
Size: 1064 138 3602 374 146 1280
Fragments arranged by size:
3602 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3006 4667 5264 6051 6319 1239
Size: 393 1374 1661 597 787 268 1524
Fragments arranged by size:
1661 1524 1374 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2032 2494 2032
Size: 462 6142
EcoO109I rG'GnC_Cy
Cuts at: 2117 2117
Size: 6604
EcoRI G'AATT_C
Cuts at: 2633 3913 4291 2633
Size: 1280 378 4946
Fragments arranged by size:
4946 1280 378
EcoRV GAT'ATC
Cuts at: 2440 2440
Size: 6604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2480 2480
Size: 6604
FspI TGC'GCA
Cuts at: 5110 5110
Size: 6604
HaeII r_GCGC'y
Cuts at: 1678 2084 5751 1678
Size: 406 3667 2531
Fragments arranged by size:
3667 2531 406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 5245 5277 5319
Size: 32 135 32 857 32 2784 32 42
Cuts at: 5319 5351 1373
Size: 32 2626
Fragments arranged by size:
2784 2626 857 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3068 3948 4194 669
Size: 988 281 1130 880 246 3079
Fragments arranged by size:
3079 1130 988 880 281 246
HindIII A'AGCT_T
Cuts at: 748 2267 2477 748
Size: 1519 210 4875
Fragments arranged by size:
4875 1519 210
HpaI GTT'AAC
Cuts at: 4194 4194
Size: 6604
KpnI G_GTAC'C
Cuts at: 3929 3929
Size: 6604
MfeI C'AATT_G
Cuts at: 2727 3748 3899 4203 2727
Size: 1021 151 304 5128
Fragments arranged by size:
5128 1021 304 151
MluI A'CGCG_T
Cuts at: 6480 6480
Size: 6604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3013 5718 5902 1305
Size: 1708 2705 184 2007
Fragments arranged by size:
2705 2007 1708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4782
Fragments arranged by size:
4782 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2573 3584 3814 4562 4921 5080 518
Size: 648 1407 1011 230 748 359 159 2042
Fragments arranged by size:
2042 1407 1011 748 648 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 2064 4020 4697 5845 6090 1606
Size: 458 1956 677 1148 245 2120
Fragments arranged by size:
2120 1956 1148 677 458 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6052
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6604
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6604
NotI GC'GGCC_GC
Cuts at: 4051 4051
Size: 6604
NsiI A_TGCA'T
Cuts at: 2534 2762 3602 2534
Size: 228 840 5536
Fragments arranged by size:
5536 840 228
NspI r_CATG'y
Cuts at: 1132 1229 2393 3962 5507 1132
Size: 97 1164 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 1164 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6604
PciI A'CATG_T
Cuts at: 2389 5503 2389
Size: 3114 3490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6604
PmeI GTTT'AAAC
Cuts at: 3908 3908
Size: 6604
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 6186 6218 4673
Size: 32 1481 32 5059
Fragments arranged by size:
5059 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6604
PsiI TTA'TAA
Cuts at: 2745 3465 4174 2745
Size: 720 709 5175
Fragments arranged by size:
5175 720 709
PspOMI G'GGCC_C
Cuts at: 2116 2116
Size: 6604
PstI C_TGCA'G
Cuts at: 830 2665 3956 830
Size: 1835 1291 3478
Fragments arranged by size:
3478 1835 1291
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6604
PvuII CAG'CTG
Cuts at: 2064 2064
Size: 6604
SalI G'TCGA_C
Cuts at: 1936 3946 1936
Size: 2010 4594
SbfI CC_TGCA'GG
Cuts at: 3956 3956
Size: 6604
ScaI AGT'ACT
Cuts at: 3868 4852 6564 3868
Size: 984 1712 3908
Fragments arranged by size:
3908 1712 984
SfcI C'TryA_G
Cuts at: 826 1045 2661 3952 5087 5768 5959 826
Size: 219 1616 1291 1135 681 191 1471
Fragments arranged by size:
1616 1471 1291 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6604
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6604
SmaI CCC'GGG
Cuts at: 1872 3931 1872
Size: 2059 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2047 4708 5609 5871 820
Size: 197 926 104 2661 901 262 1553
Fragments arranged by size:
2661 1553 926 901 262 197 104
SnaBI TAC'GTA
Cuts at: 493 4395 493
Size: 3902 2702
SpeI A'CTAG_T
Cuts at: 152 2653 3620 152
Size: 2501 967 3136
Fragments arranged by size:
3136 2501 967
SphI G_CATG'C
Cuts at: 1132 3962 1132
Size: 2830 3774
SspI AAT'ATT
Cuts at: 5 52 3568 4528 5
Size: 47 3516 960 2081
Fragments arranged by size:
3516 2081 960 47
StuI AGG'CCT
Cuts at: 1517 2027 1517
Size: 510 6094
StyI C'CwwG_G
Cuts at: 513 1065 2273 2692 4043 513
Size: 552 1208 419 1351 3074
Fragments arranged by size:
3074 1351 1208 552 419
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4631 4648 4801 4986 5431 325
Size: 1255 3051 17 153 185 445 1498
Fragments arranged by size:
3051 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2558 3089 3866
Size: 274 80 33 51 1443 581 531 777
Cuts at: 3866 4850 6562 96
Size: 984 1712 138
Fragments arranged by size:
1712 1443 984 777 581 531 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2970 3002 6564 6596 2970
Size: 32 3562 32 2978
Fragments arranged by size:
3562 2978 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6604
XbaI T'CTAG_A
Cuts at: 3940 3940
Size: 6604
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6604
XmaI C'CCGG_G
Cuts at: 1870 3929 1870
Size: 2059 4545
XmnI GAAnn'nnTTC
Cuts at: 2261 3894 4733 2261
Size: 1633 839 4132
Fragments arranged by size:
4132 1633 839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4412 276
Size: 53 83 186 936 2878 2468
Fragments arranged by size:
2878 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI