Restriction map for
pFN21AE4170
(Circular) (Six-base) MAPSORT of: pFN21AE4170.seq Check: 5403 from: 1 to: 6568
pFN21AE4170 6568 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3911 1937
Size: 1974 4594
Acc65I G'GTAC_C
Cuts at: 3889 3889
Size: 6568
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2529 3134 3347 4631 6015 6283 1632
Size: 897 605 213 1284 1384 268 1917
Fragments arranged by size:
1917 1384 1284 897 605 268 213
AflII C'TTAA_G
Cuts at: 820 1017 3733 820
Size: 197 2716 3655
Fragments arranged by size:
3655 2716 197
AflIII A'CryG_T
Cuts at: 1203 2425 5467 6444 1203
Size: 1222 3042 977 1327
Fragments arranged by size:
3042 1327 1222 977
AgeI A'CCGG_T
Cuts at: 2011 5399 2011
Size: 3388 3180
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2938 1184
Size: 1754 4814
AlwNI CAG_nnn'CTG
Cuts at: 3694 4311 5883 3694
Size: 617 1572 4379
Fragments arranged by size:
4379 1572 617
ApaLI G'TGCA_C
Cuts at: 4625 5781 4625
Size: 1156 5412
ApoI r'AATT_y
Cuts at: 1493 2233 2463 2599 3017 3877 4103 4255 6364
Size: 740 230 136 418 860 226 152 2109
Cuts at: 6364 1493
Size: 1697
Fragments arranged by size:
2109 1697 860 740 418 230 226 152
136
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 2816 3893 1870
Size: 73 873 1077 4545
Fragments arranged by size:
4545 1077 873 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2393 2426 2393
Size: 33 6535
BamHI G'GATC_C
Cuts at: 3125 3898 4265 3125
Size: 773 367 5428
Fragments arranged by size:
5428 773 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 3546 3889 5344 618
Size: 325 217 627 277 1482 343 1455 1842
Fragments arranged by size:
1842 1482 1455 627 343 325 277 217
BanII G_rGCy'C
Cuts at: 1391 3887 1391
Size: 2496 4072
BbeI G_GCGC'C
Cuts at: 2068 2068
Size: 6568
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3405 928
Size: 971 1506 4091
Fragments arranged by size:
4091 1506 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2421 4460 5670 2421
Size: 2039 1210 3319
Fragments arranged by size:
3319 2039 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6568
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5179 136
Size: 107 122 71 631 605 3507 1525
Fragments arranged by size:
3507 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3635 3760 6563 3635
Size: 125 2803 3640
Fragments arranged by size:
3640 2803 125
BlpI GC'TnA_GC
Cuts at: 3335 3985 3335
Size: 650 5918
Bme1580I G_kGCm'C
Cuts at: 1790 3551 4629 5785 1790
Size: 1761 1078 1156 2573
Fragments arranged by size:
2573 1761 1156 1078
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2998 4299 5257
Size: 416 53 83 399 24 1581 1301 958
Cuts at: 5257 442
Size: 1753
Fragments arranged by size:
1753 1581 1301 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3420 1056
Size: 2364 4204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3879 3879
Size: 6568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3545 5228 1239
Size: 2306 1683 2579
Fragments arranged by size:
2579 2306 1683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2696 3237 4693 5558 5856 2696
Size: 541 1456 865 298 3408
Fragments arranged by size:
3408 1456 865 541 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2533 5231 882
Size: 617 1034 2698 2219
Fragments arranged by size:
2698 2219 1034 617
BsaAI yAC'GTr
Cuts at: 493 1204 4359 493
Size: 711 3155 2702
Fragments arranged by size:
3155 2702 711
BsaBI GATnn'nnATC
Cuts at: 6562 6562
Size: 6568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2065 2354 4375 4757
Size: 53 83 186 936 532 289 2021 382
Cuts at: 4757 5392 275
Size: 635 1451
Fragments arranged by size:
2021 1451 936 635 532 382 289 186
83 53
BsaWI w'CCGG_w
Cuts at: 2011 3447 5001 5399 5673 5820 6231 2011
Size: 1436 1554 398 274 147 411 2348
Fragments arranged by size:
2348 1554 1436 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3582 3612 1178
Size: 30 165 30 2179 30 4134
Fragments arranged by size:
4134 2179 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2103 3220 3542 1186
Size: 917 1117 322 4212
Fragments arranged by size:
4212 1117 917 322
BseYI C'CCAG_C
Cuts at: 5771 6267 5771
Size: 496 6072
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3019 1163
Size: 573 1283 4712
Fragments arranged by size:
4712 1283 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4018 4779 5807 1540
Size: 275 186 2017 761 1028 2301
Fragments arranged by size:
2301 2017 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4629 4714 5785 4629
Size: 85 1071 5412
Fragments arranged by size:
5412 1071 85
BsmI GAATG_Cn'
Cuts at: 2560 3829 4079 4172 2560
Size: 1269 250 93 4956
Fragments arranged by size:
4956 1269 250 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3551 3887 4629 4714 5785 1391
Size: 399 1761 336 742 85 1071 2174
Fragments arranged by size:
2174 1761 1071 742 399 336 85
BspEI T'CCGG_A
Cuts at: 3447 6231 3447
Size: 2784 3784
BspHI T'CATG_A
Cuts at: 3390 4457 6187 3390
Size: 1067 1730 3771
Fragments arranged by size:
3771 1730 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3096 3923 844
Size: 2252 827 3489
Fragments arranged by size:
3489 2252 827
BsrBI CCG'CTC
Cuts at: 1394 1823 4455 1394
Size: 429 2632 3507
Fragments arranged by size:
3507 2632 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2941 3267 5063 5237 65
Size: 1183 1693 326 1796 174 1396
Fragments arranged by size:
1796 1693 1396 1183 326 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 5212 5399 1700
Size: 110 201 3201 187 2869
Fragments arranged by size:
3201 2869 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 2422 96
Size: 2326 4242
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6568
BssSI C'ACGA_G
Cuts at: 4628 5640 4628
Size: 1012 5556
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3573 794
Size: 2779 3789
BstBI TT'CG_AA
Cuts at: 2296 5372 2296
Size: 3076 3492
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6568
BstYI r'GATC_y
Cuts at: 1972 3125 3457 3635 3760 3898 4265 4650 4667
Size: 1153 332 178 125 138 367 385 17
Cuts at: 4667 6119 6205 6217 6563 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1153 385 367 346 332 178
138 125 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6016
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5829
Fragments arranged by size:
5829 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3329 4084 4878 4898 2812
Size: 517 755 794 20 4482
Fragments arranged by size:
4482 794 755 517 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3872 3872
Size: 6568
DraIII CAC_nnn'GTG
Cuts at: 3573 3573
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4015 4904 6533
Size: 54 1425 50 275 18 2185 889 1629
Cuts at: 6533 8
Size: 43
Fragments arranged by size:
2185 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4015 1537
Size: 275 2203 4090
Fragments arranged by size:
4090 2203 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 4498 1353
Size: 1293 1852 3423
Fragments arranged by size:
3423 1852 1293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5155 5529 5675 387
Size: 1064 138 3566 374 146 1280
Fragments arranged by size:
3566 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2529 3134 3347 3545 4631 5228 6015
Size: 393 897 605 213 198 1086 597 787
Cuts at: 6015 6283 1239
Size: 268 1524
Fragments arranged by size:
1524 1086 897 787 605 597 393 268
213 198
EcoRI G'AATT_C
Cuts at: 2463 3877 4255 2463
Size: 1414 378 4776
Fragments arranged by size:
4776 1414 378
FspI TGC'GCA
Cuts at: 5074 5074
Size: 6568
HaeII r_GCGC'y
Cuts at: 1678 2068 5715 1678
Size: 390 3647 2531
Fragments arranged by size:
3647 2531 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 2866 2898 3069
Size: 32 135 32 553 32 709 32 171
Cuts at: 3069 3101 3285 3317 3442 3474 5209 5241 5283
Size: 32 184 32 125 32 1735 32 42
Cuts at: 5283 5315 1373
Size: 32 2626
Fragments arranged by size:
2626 1735 709 553 184 171 135 125
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 3912 4158 669
Size: 988 281 90 1884 246 3079
Fragments arranged by size:
3079 1884 988 281 246 90
HindIII A'AGCT_T
Cuts at: 748 3255 3829 748
Size: 2507 574 3487
Fragments arranged by size:
3487 2507 574
HpaI GTT'AAC
Cuts at: 2028 4158 2028
Size: 2130 4438
KasI G'GCGC_C
Cuts at: 2064 2064
Size: 6568
KpnI G_GTAC'C
Cuts at: 3893 3893
Size: 6568
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5682 5866 1305
Size: 4377 184 2007
Fragments arranged by size:
4377 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4746
Fragments arranged by size:
4746 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2946 4526 4885 5044 518
Size: 648 1780 1580 359 159 2042
Fragments arranged by size:
2042 1780 1580 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2288 2420 2507 2872 3694 3984 4661 5809
Size: 682 132 87 365 822 290 677 1148
Cuts at: 5809 6054 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 822 682 677 365 290 245
132 87
NarI GG'CG_CC
Cuts at: 2065 2065
Size: 6568
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6016
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 3416 1052
Size: 2364 4204
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 2429 3672 3926 5471 1132
Size: 97 1200 1243 254 1545 2229
Fragments arranged by size:
2229 1545 1243 1200 254 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6568
PciI A'CATG_T
Cuts at: 2425 5467 2425
Size: 3042 3526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2267 2834 1108
Size: 1159 567 4842
Fragments arranged by size:
4842 1159 567
PfoI T'CCnGG_A
Cuts at: 2446 3744 2446
Size: 1298 5270
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 6150 6182 4637
Size: 32 1481 32 5023
Fragments arranged by size:
5023 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6568
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PstI C_TGCA'G
Cuts at: 830 3561 3920 830
Size: 2731 359 3478
Fragments arranged by size:
3478 2731 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 2288 2420 2507 2872 3694 2288
Size: 132 87 365 822 5162
Fragments arranged by size:
5162 822 365 132 87
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SexAI A'CCwGG_T
Cuts at: 3680 3680
Size: 6568
SfcI C'TryA_G
Cuts at: 826 1045 2115 2415 3023 3212 3557 3916 5051
Size: 219 1070 300 608 189 345 359 1135
Cuts at: 5051 5732 5923 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1070 681 608 359 345 300
219 191 189
SfoI GGC'GCC
Cuts at: 2066 2066
Size: 6568
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2711 3252 3733 4672 5573 5835
Size: 197 926 768 541 481 939 901 262
Cuts at: 5835 820
Size: 1553
Fragments arranged by size:
1553 939 926 901 768 541 481 262
197
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 2651 3478 4492 5
Size: 47 2599 827 1014 2081
Fragments arranged by size:
2599 2081 1014 827 47
StuI AGG'CCT
Cuts at: 1517 3218 1517
Size: 1701 4867
StyI C'CwwG_G
Cuts at: 513 1065 3538 4007 513
Size: 552 2473 469 3074
Fragments arranged by size:
3074 2473 552 469
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2120 3501 4595 4612 4765 4950 5395
Size: 1255 540 1381 1094 17 153 185 445
Cuts at: 5395 325
Size: 1498
Fragments arranged by size:
1498 1381 1255 1094 540 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2422 4814 6526
Size: 274 80 33 51 1443 445 2392 1712
Cuts at: 6526 96
Size: 138
Fragments arranged by size:
2392 1712 1443 445 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3582 3614 6528 6560 3582
Size: 32 2914 32 3590
Fragments arranged by size:
3590 2914 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6568
XbaI T'CTAG_A
Cuts at: 2431 3904 2431
Size: 1473 5095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2176
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 4697 4697
Size: 6568
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI