Restriction map for
pFN21AE4169
(Circular) (Six-base) MAPSORT of: pFN21AE4169.seq Check: 4310 from: 1 to: 6568
pFN21AE4169 6568 bp
With 182 enzymes: SgfI *
March 28, 2012 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3806 3806
Size: 6568
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3396 3911 1937
Size: 1459 515 4594
Fragments arranged by size:
4594 1459 515
Acc65I G'GTAC_C
Cuts at: 3674 3889 3674
Size: 215 6353
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4631 6015 6283 1632
Size: 2999 1384 268 1917
Fragments arranged by size:
2999 1917 1384 268
AfeI AGC'GCT
Cuts at: 3284 3284
Size: 6568
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 5467 6444 1203
Size: 4264 977 1327
Fragments arranged by size:
4264 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 3316 5297 3316
Size: 1981 4587
AleI CACnn'nnGTG
Cuts at: 1166 2489 1166
Size: 1323 5245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2966 1184
Size: 1782 4786
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2570 2602 3714 3746 2570
Size: 32 1112 32 5392
Fragments arranged by size:
5392 1112 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2260 4311 5883 2260
Size: 2051 1572 2945
Fragments arranged by size:
2945 2051 1572
ApaLI G'TGCA_C
Cuts at: 4625 5781 4625
Size: 1156 5412
ApoI r'AATT_y
Cuts at: 1493 3877 4103 4255 6364 1493
Size: 2384 226 152 2109 1697
Fragments arranged by size:
2384 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 3893 1870
Size: 73 804 1146 4545
Fragments arranged by size:
4545 1146 804 73
BamHI G'GATC_C
Cuts at: 2916 3898 4265 2916
Size: 982 367 5219
Fragments arranged by size:
5219 982 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3484 3616 3674 3818 3889
Size: 325 217 627 1697 132 58 144 71
Cuts at: 3889 5344 618
Size: 1455 1842
Fragments arranged by size:
1842 1697 1455 627 325 217 144 132
71 58
BanII G_rGCy'C
Cuts at: 1391 2073 2535 3744 3887 1391
Size: 682 462 1209 143 4072
Fragments arranged by size:
4072 1209 682 462 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2404 2656 3079 3123 928
Size: 971 505 252 423 44 4373
Fragments arranged by size:
4373 971 505 423 252 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2083 2117 4759 4793 1903
Size: 34 146 34 2642 34 3678
Fragments arranged by size:
3678 2642 146 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2915 4460 5670 2915
Size: 1545 1210 3813
Fragments arranged by size:
3813 1545 1210
BclI T'GATC_A
Cuts at: 1548 2970 1548
Size: 1422 5146
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2849 3219 5179
Size: 107 122 71 631 605 1177 370 1960
Cuts at: 5179 136
Size: 1525
Fragments arranged by size:
1960 1525 1177 631 605 370 122 107
71
BglII A'GATC_T
Cuts at: 6563 6563
Size: 6568
BlpI GC'TnA_GC
Cuts at: 2637 3985 2637
Size: 1348 5220
Bme1580I G_kGCm'C
Cuts at: 1790 2743 2864 3619 4629 5785 1790
Size: 953 121 755 1010 1156 2573
Fragments arranged by size:
2573 1156 1010 953 755 121
BmgBI CAC'GTC
Cuts at: 1318 2245 3058 1318
Size: 927 813 4828
Fragments arranged by size:
4828 927 813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4299 5257 442
Size: 416 53 83 399 24 2882 958 1753
Fragments arranged by size:
2882 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3664 3251
Size: 413 6155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2533 3117 3728 3741 3775 5228 1239
Size: 1007 287 584 611 13 34 1453 2579
Fragments arranged by size:
2579 1453 1007 611 584 287 34 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4693 5558 5856 4693
Size: 865 298 5405
Fragments arranged by size:
5405 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5231 882
Size: 617 3732 2219
Fragments arranged by size:
3732 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3250 3355 3400 4359 493
Size: 711 2046 105 45 959 2702
Fragments arranged by size:
2702 2046 959 711 105 45
BsaBI GATnn'nnATC
Cuts at: 6562 6562
Size: 6568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 4375 4757 5392
Size: 53 83 186 936 1089 1753 382 635
Cuts at: 5392 275
Size: 1451
Fragments arranged by size:
1753 1451 1089 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5001 5399 5673 5820 6231 5001
Size: 398 274 147 411 5338
Fragments arranged by size:
5338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 2219 2249 2570
Size: 30 165 30 621 30 165 30 321
Cuts at: 2570 2600 3701 3714 3731 3744 1178
Size: 30 1101 13 17 13 4002
Fragments arranged by size:
4002 1101 621 321 165 165 30 30
30 30 30 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2243 2591 2897 2906 2993 3782 3785
Size: 859 198 348 306 9 87 789 3
Cuts at: 3785 1186
Size: 3969
Fragments arranged by size:
3969 859 789 348 306 198 87 9
3
BseYI C'CCAG_C
Cuts at: 2011 2742 3181 3551 5771 6267 2011
Size: 731 439 370 2220 496 2312
Fragments arranged by size:
2312 2220 731 496 439 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2246 2327 2786 1163
Size: 573 468 42 81 459 4945
Fragments arranged by size:
4945 573 468 459 81 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2526 4018 4779 5807 1540
Size: 275 186 525 1492 761 1028 2301
Fragments arranged by size:
2301 1492 1028 761 525 275 186
BsiHKAI G_wGCw'C
Cuts at: 2535 3378 3744 4629 4714 5785 2535
Size: 843 366 885 85 1071 3318
Fragments arranged by size:
3318 1071 885 843 366 85
BsmI GAATG_Cn'
Cuts at: 4079 4172 4079
Size: 93 6475
BsmBI CGTCTCn'nnnn_
Cuts at: 2423 2450 3704 2423
Size: 27 1254 5287
Fragments arranged by size:
5287 1254 27
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2535 2743 2864 3378 3619 3744
Size: 399 283 462 208 121 514 241 125
Cuts at: 3744 3887 4629 4714 5785 1391
Size: 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 462 399 283 241
208 143 125 121 85
BspEI T'CCGG_A
Cuts at: 6231 6231
Size: 6568
BspHI T'CATG_A
Cuts at: 2967 4457 6187 2967
Size: 1490 1730 3348
Fragments arranged by size:
3348 1730 1490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3806 3923 844
Size: 2962 117 3489
Fragments arranged by size:
3489 2962 117
BsrBI CCG'CTC
Cuts at: 1394 1823 3504 4455 1394
Size: 429 1681 951 3507
Fragments arranged by size:
3507 1681 951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5063 5237 65
Size: 1183 3815 174 1396
Fragments arranged by size:
3815 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5212 5399 1700
Size: 110 3402 187 2869
Fragments arranged by size:
3402 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2271 3591 96
Size: 2175 1320 3073
Fragments arranged by size:
3073 2175 1320
BssHII G'CGCG_C
Cuts at: 1925 2183 2206 2439 2495 2647 2649 2695 1925
Size: 258 23 233 56 152 2 46 5798
Fragments arranged by size:
5798 258 233 152 56 46 23 2
BssSI C'ACGA_G
Cuts at: 2732 2786 4628 5640 2732
Size: 54 1842 1012 3660
Fragments arranged by size:
3660 1842 1012 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3818 794
Size: 3024 3544
BstBI TT'CG_AA
Cuts at: 5372 5372
Size: 6568
BstEII G'GTnAC_C
Cuts at: 1184 3067 3556 1184
Size: 1883 489 4196
Fragments arranged by size:
4196 1883 489
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2404 3250 2011
Size: 393 846 5329
Fragments arranged by size:
5329 846 393
BstYI r'GATC_y
Cuts at: 1972 2340 2916 2956 3898 4265 4650 4667 6119
Size: 368 576 40 942 367 385 17 1452
Cuts at: 6119 6205 6217 6563 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 942 576 385 368 367 346
86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2089 2322 2451 513
Size: 552 939 85 233 129 4630
Fragments arranged by size:
4630 939 552 233 129 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2129 2521 487
Size: 683 56 903 392 4534
Fragments arranged by size:
4534 903 683 392 56
BtsI GCAGTG_nn'
Cuts at: 2589 3424 4084 4878 4898 2589
Size: 835 660 794 20 4259
Fragments arranged by size:
4259 835 794 660 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 3872 3872
Size: 6568
DraIII CAC_nnn'GTG
Cuts at: 3373 3373
Size: 6568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5575 809
Size: 507 4259 1802
Fragments arranged by size:
4259 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2406 2523
Size: 54 1425 50 275 18 327 249 117
Cuts at: 2523 3364 3532 4015 4904 6533 8
Size: 841 168 483 889 1629 43
Fragments arranged by size:
1629 1425 889 841 483 327 275 249
168 117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2523 4015 1537
Size: 275 711 1492 4090
Fragments arranged by size:
4090 1492 711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3110 4498 1353
Size: 1757 1388 3423
Fragments arranged by size:
3423 1757 1388
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 5155 5529 5675 387
Size: 1064 138 453 3113 374 146 1280
Fragments arranged by size:
3113 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 2533 3742 2533
Size: 1209 5359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2533 3117 3728 3741 3775 4631
Size: 393 614 287 584 611 13 34 856
Cuts at: 4631 5228 6015 6283 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 856 787 614 611 597 584 393
287 268 34 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 2228
Size: 6568
EcoO109I rG'GnC_Cy
Cuts at: 3043 3130 3043
Size: 87 6481
EcoRI G'AATT_C
Cuts at: 3877 4255 3877
Size: 378 6190
FspI TGC'GCA
Cuts at: 2759 5074 2759
Size: 2315 4253
HaeII r_GCGC'y
Cuts at: 1678 2012 2386 2644 2725 2988 3286 5715 1678
Size: 334 374 258 81 263 298 2429 2531
Fragments arranged by size:
2531 2429 374 334 298 263 258 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 3302 3334 3701
Size: 32 135 32 647 32 1051 32 367
Cuts at: 3701 3733 5209 5241 5283 5315 1373
Size: 32 1476 32 42 32 2626
Fragments arranged by size:
2626 1476 1051 647 367 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3912 4158 669
Size: 988 281 1974 246 3079
Fragments arranged by size:
3079 1974 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6568
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KpnI G_GTAC'C
Cuts at: 3678 3893 3678
Size: 215 6353
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 3060 5682 5866 1305
Size: 1116 639 2622 184 2007
Fragments arranged by size:
2622 2007 1116 639 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3366 10
Size: 54 1425 343 1534 3212
Fragments arranged by size:
3212 1534 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 2489 2966 3248 3325 3542 4526
Size: 648 1197 126 477 282 77 217 984
Cuts at: 4526 4885 5044 518
Size: 359 159 2042
Fragments arranged by size:
2042 1197 984 648 477 359 282 217
159 126 77
MspA1I CmG'CkG
Cuts at: 1606 2038 2324 3196 3210 3346 3551 3984 4661
Size: 432 286 872 14 136 205 433 677
Cuts at: 4661 5809 6054 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 872 677 433 432 286 245
205 136 14
NcoI C'CATG_G
Cuts at: 513 1065 2004 2089 513
Size: 552 939 85 4992
Fragments arranged by size:
4992 939 552 85
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 2083 2947 3926 5471 1132
Size: 97 854 864 979 1545 2229
Fragments arranged by size:
2229 1545 979 864 854 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6568
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 1108
Size: 1041 5527
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2117 2149 2570 2602 3714 3746 4637 4669 6150
Size: 32 421 32 1112 32 891 32 1481
Cuts at: 6150 6182 2117
Size: 32 2503
Fragments arranged by size:
2503 1481 1112 891 421 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3043 3130 3043
Size: 87 6481
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6568
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PstI C_TGCA'G
Cuts at: 830 2168 2379 2955 3156 3920 830
Size: 1338 211 576 201 764 3478
Fragments arranged by size:
3478 1338 764 576 211 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6568
PvuII CAG'CTG
Cuts at: 3551 3551
Size: 6568
SacI G_AGCT'C
Cuts at: 2535 3744 2535
Size: 1209 5359
SacII CC_GC'GG
Cuts at: 2325 2325
Size: 6568
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SbfI CC_TGCA'GG
Cuts at: 2955 3920 2955
Size: 965 5603
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SexAI A'CCwGG_T
Cuts at: 3039 3081 3039
Size: 42 6526
SfcI C'TryA_G
Cuts at: 826 1045 2164 2375 2951 3152 3916 5051 5732
Size: 219 1119 211 576 201 764 1135 681
Cuts at: 5732 5923 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1119 764 681 576 219 211
201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4672 5573 5835 820
Size: 197 926 2729 901 262 1553
Fragments arranged by size:
2729 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3400 4359 493
Size: 2907 959 2702
Fragments arranged by size:
2907 2702 959
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 3926 1132
Size: 2794 3774
SspI AAT'ATT
Cuts at: 5 52 4492 5
Size: 47 4440 2081
Fragments arranged by size:
4440 2081 47
StuI AGG'CCT
Cuts at: 1517 3102 3629 1517
Size: 1585 527 4456
Fragments arranged by size:
4456 1585 527
StyI C'CwwG_G
Cuts at: 513 1065 2004 2089 2844 3072 4007 513
Size: 552 939 85 755 228 935 3074
Fragments arranged by size:
3074 939 935 755 552 228 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3286 3502 4595 4612 4765 4950 5395
Size: 1255 1706 216 1093 17 153 185 445
Cuts at: 5395 325
Size: 1498
Fragments arranged by size:
1706 1498 1255 1093 445 216 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 3591 4814
Size: 274 80 33 51 1443 294 1320 1223
Cuts at: 4814 6526 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1320 1223 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 3161 3193 6528 6560 2319
Size: 32 810 32 3335 32 2327
Fragments arranged by size:
3335 2327 810 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3296 3392 3715 1316
Size: 1980 96 323 4169
Fragments arranged by size:
4169 1980 323 96
XbaI T'CTAG_A
Cuts at: 3904 3904
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 4697 4697
Size: 6568
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI
SrfI SwaI XcmI