Restriction map for
pFN21AE4164
(Circular) (Six-base) MAPSORT of: pFN21AE4164.seq Check: 1381 from: 1 to: 6529
pFN21AE4164 6529 bp
With 182 enzymes: SgfI *
March 28, 2012 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3614 3614
Size: 6529
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4339 278
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3705 3872 1937
Size: 1768 167 4594
Fragments arranged by size:
4594 1768 167
Acc65I G'GTAC_C
Cuts at: 3850 3850
Size: 6529
AclI AA'CG_TT
Cuts at: 1563 4656 5029 1563
Size: 3093 373 3063
Fragments arranged by size:
3093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 2729 4592 5976 6244 1632
Size: 1011 86 1863 1384 268 1917
Fragments arranged by size:
1917 1863 1384 1011 268 86
AflII C'TTAA_G
Cuts at: 820 1017 2107 820
Size: 197 1090 5242
Fragments arranged by size:
5242 1090 197
AflIII A'CryG_T
Cuts at: 1203 2039 5428 6405 1203
Size: 836 3389 977 1327
Fragments arranged by size:
3389 1327 977 836
AgeI A'CCGG_T
Cuts at: 5360 5360
Size: 6529
AhdI GACnn_n'nnGTC
Cuts at: 5258 5258
Size: 6529
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3361 1184
Size: 2177 4352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 2741
Size: 32 6497
AlwNI CAG_nnn'CTG
Cuts at: 4272 5844 4272
Size: 1572 4957
ApaLI G'TGCA_C
Cuts at: 4586 5742 4586
Size: 1156 5373
ApoI r'AATT_y
Cuts at: 1493 2049 2122 2572 3648 3764 3838 4064 4216
Size: 556 73 450 1076 116 74 226 152
Cuts at: 4216 6325 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1076 556 450 226 152 116
74 73
AseI AT'TA_AT
Cuts at: 160 1032 2439 3494 5083 160
Size: 872 1407 1055 1589 1606
Fragments arranged by size:
1606 1589 1407 1055 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
AvaI C'yCGr_G
Cuts at: 1870 1943 3237 3854 1870
Size: 73 1294 617 4545
Fragments arranged by size:
4545 1294 617 73
BamHI G'GATC_C
Cuts at: 3233 3859 4226 3233
Size: 626 367 5536
Fragments arranged by size:
5536 626 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3850 5305 618
Size: 325 217 627 2063 1455 1842
Fragments arranged by size:
2063 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3848 1391
Size: 2457 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4720 4754 1903
Size: 34 2783 34 3678
Fragments arranged by size:
3678 2783 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4421 5631 4421
Size: 1210 5319
BclI T'GATC_A
Cuts at: 1548 2503 3103 3442 1548
Size: 955 600 339 4635
Fragments arranged by size:
4635 955 600 339
BfrBI ATG'CAT
Cuts at: 2510 3178 2510
Size: 668 5861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5140 136
Size: 107 122 71 631 605 3468 1525
Fragments arranged by size:
3468 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6524 6524
Size: 6529
BlpI GC'TnA_GC
Cuts at: 3946 3946
Size: 6529
Bme1580I G_kGCm'C
Cuts at: 1790 4590 5746 1790
Size: 2800 1156 2573
Fragments arranged by size:
2800 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4260 5218 442
Size: 416 53 83 399 24 2843 958 1753
Fragments arranged by size:
2843 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5189 1239
Size: 3950 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4654 5519 5817 4654
Size: 865 298 5366
Fragments arranged by size:
5366 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3795 5192 882
Size: 617 2296 1397 2219
Fragments arranged by size:
2296 2219 1397 617
BsaAI yAC'GTr
Cuts at: 493 1204 4320 493
Size: 711 3116 2702
Fragments arranged by size:
3116 2702 711
BsaBI GATnn'nnATC
Cuts at: 6523 6523
Size: 6529
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4336 4718 5353 275
Size: 53 83 186 936 2803 382 635 1451
Fragments arranged by size:
2803 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4962 5360 5634 5781 6192 4962
Size: 398 274 147 411 5299
Fragments arranged by size:
5299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6304
Fragments arranged by size:
6304 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2750 1186
Size: 1564 4965
BseYI C'CCAG_C
Cuts at: 5732 6228 5732
Size: 496 6033
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3630 3719 1163
Size: 573 1894 89 3973
Fragments arranged by size:
3973 1894 573 89
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3979 4740 5768 1540
Size: 275 186 1978 761 1028 2301
Fragments arranged by size:
2301 1978 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4590 4675 5746 4590
Size: 85 1071 5373
Fragments arranged by size:
5373 1071 85
BsmI GAATG_Cn'
Cuts at: 4040 4133 4040
Size: 93 6436
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3848 4590 4675 5746 1391
Size: 399 2058 742 85 1071 2174
Fragments arranged by size:
2174 2058 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6192 6192
Size: 6529
BspHI T'CATG_A
Cuts at: 3091 4418 6148 3091
Size: 1327 1730 3472
Fragments arranged by size:
3472 1730 1327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3614 3691 3884 844
Size: 2770 77 193 3489
Fragments arranged by size:
3489 2770 193 77
BsrBI CCG'CTC
Cuts at: 1394 1823 4416 1394
Size: 429 2593 3507
Fragments arranged by size:
3507 2593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5024 5198 65
Size: 1183 3776 174 1396
Fragments arranged by size:
3776 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5173 5360 1700
Size: 110 3363 187 2869
Fragments arranged by size:
3363 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2521 3518 96
Size: 2425 997 3107
Fragments arranged by size:
3107 2425 997
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6529
BssSI C'ACGA_G
Cuts at: 4589 5601 4589
Size: 1012 5517
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6529
BstBI TT'CG_AA
Cuts at: 5333 5333
Size: 6529
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6529
BstYI r'GATC_y
Cuts at: 1972 2993 3233 3596 3859 4226 4611 4628 6080
Size: 1021 240 363 263 367 385 17 1452
Cuts at: 6080 6166 6178 6524 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1021 385 367 363 346 263
240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3806 513
Size: 552 939 1802 3236
Fragments arranged by size:
3236 1802 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5790
Fragments arranged by size:
5790 683 56
BtsI GCAGTG_nn'
Cuts at: 2218 2422 4045 4839 4859 2218
Size: 204 1623 794 20 3888
Fragments arranged by size:
3888 1623 794 204 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6494
DraI TTT'AAA
Cuts at: 2548 3424 3833 2548
Size: 876 409 5244
Fragments arranged by size:
5244 876 409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5536 809
Size: 507 4220 1802
Fragments arranged by size:
4220 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3976 4865 6494
Size: 54 1425 50 275 18 2146 889 1629
Cuts at: 6494 8
Size: 43
Fragments arranged by size:
2146 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3976 1537
Size: 275 2164 4090
Fragments arranged by size:
4090 2164 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2760 2849 4459 1353
Size: 1407 89 1610 3423
Fragments arranged by size:
3423 1610 1407 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5116 5490 5636 387
Size: 1064 138 3527 374 146 1280
Fragments arranged by size:
3527 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2643 2729 4592 5189 5976 6244 1239
Size: 393 1011 86 1863 597 787 268 1524
Fragments arranged by size:
1863 1524 1011 787 597 393 268 86
EcoRI G'AATT_C
Cuts at: 2049 3838 4216 2049
Size: 1789 378 4362
Fragments arranged by size:
4362 1789 378
EcoRV GAT'ATC
Cuts at: 3297 3297
Size: 6529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2666 2666
Size: 6529
FspI TGC'GCA
Cuts at: 5035 5035
Size: 6529
HaeII r_GCGC'y
Cuts at: 1678 5676 1678
Size: 3998 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2088 2120 2707 2739 3195
Size: 32 135 32 516 32 587 32 456
Cuts at: 3195 3227 3251 3283 3434 3466 5170 5202 5244
Size: 32 24 32 151 32 1704 32 42
Cuts at: 5244 5276 1373
Size: 32 2626
Fragments arranged by size:
2626 1704 587 516 456 151 135 42
32 32 32 32 32 32 32 32
32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 3873 4119 669
Size: 988 281 1935 246 3079
Fragments arranged by size:
3079 1935 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2409 748
Size: 1661 4868
HpaI GTT'AAC
Cuts at: 4119 4119
Size: 6529
KpnI G_GTAC'C
Cuts at: 3854 3854
Size: 6529
MfeI C'AATT_G
Cuts at: 2449 3630 4128 2449
Size: 1181 498 4850
Fragments arranged by size:
4850 1181 498
MluI A'CGCG_T
Cuts at: 6405 6405
Size: 6529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2636 5643 5827 1305
Size: 1331 3007 184 2007
Fragments arranged by size:
3007 2007 1331 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4707
Fragments arranged by size:
4707 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2193 2804 4487 4846 5005 518
Size: 648 1027 611 1683 359 159 2042
Fragments arranged by size:
2042 1683 1027 648 611 359 159
MspA1I CmG'CkG
Cuts at: 1606 3945 4622 5770 6015 1606
Size: 2339 677 1148 245 2120
Fragments arranged by size:
2339 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3806 513
Size: 552 939 1802 3236
Fragments arranged by size:
3236 1802 939 552
NdeI CA'TA_TG
Cuts at: 387 2017 387
Size: 1630 4899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6529
NotI GC'GGCC_GC
Cuts at: 3976 3976
Size: 6529
NsiI A_TGCA'T
Cuts at: 2512 3180 2512
Size: 668 5861
NspI r_CATG'y
Cuts at: 1132 1229 2043 2211 3348 3887 5432 1132
Size: 97 814 168 1137 539 1545 2229
Fragments arranged by size:
2229 1545 1137 814 539 168 97
PacI TTA_AT'TAA
Cuts at: 3498 3498
Size: 6529
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6529
PciI A'CATG_T
Cuts at: 2039 5428 2039
Size: 3389 3140
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3291 1108
Size: 2183 4346
PmeI GTTT'AAAC
Cuts at: 3833 3833
Size: 6529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 6111 6143 4598
Size: 32 1481 32 4984
Fragments arranged by size:
4984 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6529
PsiI TTA'TAA
Cuts at: 4099 4099
Size: 6529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3163 3195 3348 3380 3163
Size: 32 153 32 6312
Fragments arranged by size:
6312 153 32 32
PstI C_TGCA'G
Cuts at: 830 3881 830
Size: 3051 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6529
SalI G'TCGA_C
Cuts at: 1936 3871 1936
Size: 1935 4594
SbfI CC_TGCA'GG
Cuts at: 3881 3881
Size: 6529
ScaI AGT'ACT
Cuts at: 2583 4777 6489 2583
Size: 2194 1712 2623
Fragments arranged by size:
2623 2194 1712
SexAI A'CCwGG_T
Cuts at: 3110 3110
Size: 6529
SfcI C'TryA_G
Cuts at: 826 1045 2117 2585 3877 5012 5693 5884 826
Size: 219 1072 468 1292 1135 681 191 1471
Fragments arranged by size:
1471 1292 1135 1072 681 468 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6529
SmaI CCC'GGG
Cuts at: 1872 3239 3856 1872
Size: 1367 617 4545
Fragments arranged by size:
4545 1367 617
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2107 4633 5534 5796 820
Size: 197 926 164 2526 901 262 1553
Fragments arranged by size:
2526 1553 926 901 262 197 164
SnaBI TAC'GTA
Cuts at: 493 4320 493
Size: 3827 2702
SpeI A'CTAG_T
Cuts at: 152 3457 152
Size: 3305 3224
SphI G_CATG'C
Cuts at: 1132 2211 3887 1132
Size: 1079 1676 3774
Fragments arranged by size:
3774 1676 1079
SspI AAT'ATT
Cuts at: 5 52 3024 4453 5
Size: 47 2972 1429 2081
Fragments arranged by size:
2972 2081 1429 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6529
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 3806 3968 513
Size: 552 939 288 1514 162 3074
Fragments arranged by size:
3074 1514 939 552 288 162
SwaI ATTT'AAAT
Cuts at: 2548 2548
Size: 6529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4556 4573 4726 4911 5356 325
Size: 1255 2976 17 153 185 445 1498
Fragments arranged by size:
2976 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2113 2521 2581
Size: 274 80 33 51 1443 136 408 60
Cuts at: 2581 3518 4775 6487 96
Size: 937 1257 1712 138
Fragments arranged by size:
1712 1443 1257 937 408 274 138 136
80 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6489 6521 6489
Size: 32 6497
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6529
XbaI T'CTAG_A
Cuts at: 3865 3865
Size: 6529
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6529
XmaI C'CCGG_G
Cuts at: 1870 3237 3854 1870
Size: 1367 617 4545
Fragments arranged by size:
4545 1367 617
XmnI GAAnn'nnTTC
Cuts at: 3023 4658 3023
Size: 1635 4894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4337 276
Size: 53 83 186 936 2803 2468
Fragments arranged by size:
2803 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI XcmI