Restriction map for
pFN21AE4162
(Circular) (Six-base) MAPSORT of: pFN21AE4162.seq Check: 3317 from: 1 to: 6526
pFN21AE4162 6526 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2230 2230
Size: 6526
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4336 278
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3165 3869 1937
Size: 1228 704 4594
Fragments arranged by size:
4594 1228 704
Acc65I G'GTAC_C
Cuts at: 2095 3438 3847 2095
Size: 1343 409 4774
Fragments arranged by size:
4774 1343 409
AclI AA'CG_TT
Cuts at: 1563 3474 4653 5026 1563
Size: 1911 1179 373 3063
Fragments arranged by size:
3063 1911 1179 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 2494 2708 3404 4589 5973 6241 1632
Size: 680 182 214 696 1185 1384 268 1917
Fragments arranged by size:
1917 1384 1185 696 680 268 214 182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 2016 5425 6402 1203
Size: 813 3409 977 1327
Fragments arranged by size:
3409 1327 977 813
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 2116 5255 2116
Size: 3139 3387
AleI CACnn'nnGTG
Cuts at: 1166 2364 1166
Size: 1198 5328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6526
AlwNI CAG_nnn'CTG
Cuts at: 2362 2414 4269 5841 2362
Size: 52 1855 1572 3047
Fragments arranged by size:
3047 1855 1572 52
ApaI G_GGCC'C
Cuts at: 2237 2237
Size: 6526
ApaLI G'TGCA_C
Cuts at: 4583 5739 4583
Size: 1156 5370
ApoI r'AATT_y
Cuts at: 1493 3348 3835 4061 4213 6322 1493
Size: 1855 487 226 152 2109 1697
Fragments arranged by size:
2109 1855 1697 487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 2168 3149 3851 1870
Size: 73 207 18 981 702 4545
Fragments arranged by size:
4545 981 702 207 73 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2563 2596 2563
Size: 33 6493
BamHI G'GATC_C
Cuts at: 3856 4223 3856
Size: 367 6159
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2700 3438 3847 5302
Size: 325 217 627 308 605 738 409 1455
Cuts at: 5302 618
Size: 1842
Fragments arranged by size:
1842 1455 738 627 605 409 325 308
217
BanII G_rGCy'C
Cuts at: 1391 2237 2928 3358 3791 3845 1391
Size: 846 691 430 433 54 4072
Fragments arranged by size:
4072 846 691 433 430 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3552 928
Size: 971 1653 3902
Fragments arranged by size:
3902 1653 971
BbvCI CC'TCA_GC
Cuts at: 2720 2763 2720
Size: 43 6483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2021 2055 3574 3608 4717 4751 1903
Size: 34 84 34 1519 34 1109 34 3678
Fragments arranged by size:
3678 1519 1109 84 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2564 3513 4418 5628 2564
Size: 949 905 1210 3462
Fragments arranged by size:
3462 1210 949 905
BclI T'GATC_A
Cuts at: 1548 2389 1548
Size: 841 5685
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5137 136
Size: 107 122 71 631 605 337 3128 1525
Fragments arranged by size:
3128 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6521 6521
Size: 6526
BlpI GC'TnA_GC
Cuts at: 3117 3943 3117
Size: 826 5700
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2237 2703 4587 5743 1790
Size: 431 16 466 1884 1156 2573
Fragments arranged by size:
2573 1884 1156 466 431 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2048 3631 4257
Size: 416 53 83 399 24 631 1583 626
Cuts at: 4257 5215 442
Size: 958 1753
Fragments arranged by size:
1753 1583 958 631 626 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3713
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2285 2342 2654 2666 3212 3221 3288
Size: 842 204 57 312 12 546 9 67
Cuts at: 3288 3342 5186 1239
Size: 54 1844 2579
Fragments arranged by size:
2579 1844 842 546 312 204 67 57
54 12 9
Bpu10I CC'TnA_GC
Cuts at: 2720 2763 2720
Size: 43 6483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3683 4651 5516 5814 3683
Size: 968 865 298 4395
Fragments arranged by size:
4395 968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3173 3541 5189 882
Size: 617 1674 368 1648 2219
Fragments arranged by size:
2219 1674 1648 617 368
BsaAI yAC'GTr
Cuts at: 493 1204 4317 493
Size: 711 3113 2702
Fragments arranged by size:
3113 2702 711
BsaBI GATnn'nnATC
Cuts at: 3240 6520 3240
Size: 3280 3246
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 4333 4715 5350
Size: 53 83 186 936 501 2299 382 635
Cuts at: 5350 275
Size: 1451
Fragments arranged by size:
2299 1451 936 635 501 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2047 3555 4959 5357 5631 5778 6189 2047
Size: 1508 1404 398 274 147 411 2384
Fragments arranged by size:
2384 1508 1404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6301
Fragments arranged by size:
6301 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2362 2669 2747 2894 2960 3702 3751 1186
Size: 1176 307 78 147 66 742 49 3961
Fragments arranged by size:
3961 1176 742 307 147 78 66 49
BseYI C'CCAG_C
Cuts at: 2139 3401 5729 6225 2139
Size: 1262 2328 496 2440
Fragments arranged by size:
2440 2328 1262 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2566 3014 1163
Size: 573 830 448 4675
Fragments arranged by size:
4675 830 573 448
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2209 2302 3976 4737 5765 1540
Size: 275 186 208 93 1674 761 1028 2301
Fragments arranged by size:
2301 1674 1028 761 275 208 186 93
BsiHKAI G_wGCw'C
Cuts at: 2751 2928 3358 3616 3708 4587 4672 5743 2751
Size: 177 430 258 92 879 85 1071 3534
Fragments arranged by size:
3534 1071 879 430 258 177 92 85
BsmI GAATG_Cn'
Cuts at: 4037 4130 4037
Size: 93 6433
BsmBI CGTCTCn'nnnn_
Cuts at: 2681 2681
Size: 6526
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2237 2703 2751 2928 3358 3616
Size: 399 431 16 466 48 177 430 258
Cuts at: 3616 3708 3791 3845 4587 4672 5743 1391
Size: 92 83 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 431 430 399 258
177 92 85 83 54 48 16
BspEI T'CCGG_A
Cuts at: 6189 6189
Size: 6526
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2230 3881 844
Size: 1386 1651 3489
Fragments arranged by size:
3489 1651 1386
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 4413 1394
Size: 429 1936 654 3507
Fragments arranged by size:
3507 1936 654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5021 5195 65
Size: 1183 3773 174 1396
Fragments arranged by size:
3773 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 2092 2311 3280 5170 5357 1700
Size: 110 256 26 219 969 1890 187 2869
Fragments arranged by size:
2869 1890 969 256 219 187 110 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 3615 4586 5598 3615
Size: 971 1012 4543
Fragments arranged by size:
4543 1012 971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2908 794
Size: 2114 4412
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 2663 3283 1184
Size: 1479 620 4427
Fragments arranged by size:
4427 1479 620
BstXI CCAn_nnnn'nTGG
Cuts at: 2277 2660 2277
Size: 383 6143
BstYI r'GATC_y
Cuts at: 1972 3856 4223 4608 4625 6077 6163 6175 6521
Size: 1884 367 385 17 1452 86 12 346
Cuts at: 6521 1972
Size: 1977
Fragments arranged by size:
1977 1884 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2203 513
Size: 552 939 199 4836
Fragments arranged by size:
4836 939 552 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2743 487
Size: 683 56 1517 4270
Fragments arranged by size:
4270 1517 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 2270 2554 3111 4042 4836 4856 2087
Size: 183 284 557 931 794 20 3757
Fragments arranged by size:
3757 931 794 557 284 183 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6491
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5533 809
Size: 507 4217 1802
Fragments arranged by size:
4217 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3013 3973 4862
Size: 54 1425 50 275 18 1183 960 889
Cuts at: 4862 6491 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1183 960 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3973 1537
Size: 275 2161 4090
Fragments arranged by size:
4090 2161 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3063 3423 4456 1353
Size: 1160 550 360 1033 3423
Fragments arranged by size:
3423 1160 1033 550 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3725 5113 5487 5633 387
Size: 1064 138 2136 1388 374 146 1280
Fragments arranged by size:
2136 1388 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2926 3356 2926
Size: 430 6096
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2285 2312 2342 2494 2654 2666
Size: 393 449 204 27 30 152 160 12
Cuts at: 2666 2708 3212 3221 3288 3342 3404 4589 5186
Size: 42 504 9 67 54 62 1185 597
Cuts at: 5186 5973 6241 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1185 787 597 504 449 393 268
204 160 152 67 62 54 42 30
27 12 9
EcoO109I rG'GnC_Cy
Cuts at: 2158 2165 2158
Size: 7 6519
EcoRI G'AATT_C
Cuts at: 3348 3835 4213 3348
Size: 487 378 5661
Fragments arranged by size:
5661 487 378
FseI GG_CCGG'CC
Cuts at: 2070 2070
Size: 6526
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 5673 1678
Size: 3995 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 3062 3094 5167
Size: 32 135 32 530 32 928 32 2073
Cuts at: 5167 5199 5241 5273 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2073 928 530 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2317 3145 3870 4116 669
Size: 988 281 379 828 725 246 3079
Fragments arranged by size:
3079 988 828 725 379 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6526
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KpnI G_GTAC'C
Cuts at: 2099 3442 3851 2099
Size: 1343 409 4774
Fragments arranged by size:
4774 1343 409
MfeI C'AATT_G
Cuts at: 2906 4125 2906
Size: 1219 5307
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3519 3741 5640 5824 1305
Size: 2214 222 1899 184 2007
Fragments arranged by size:
2214 2007 1899 222 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3015 10
Size: 54 1425 343 1183 3521
Fragments arranged by size:
3521 1425 1183 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 3639 4484 4843 5002 518
Size: 648 1198 1275 845 359 159 2042
Fragments arranged by size:
2042 1275 1198 845 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2205 2660 2686 2959 3199 3794 3942
Size: 495 104 455 26 273 240 595 148
Cuts at: 3942 4619 5767 6012 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 495 455 273 245
240 148 104 26
NaeI GCC'GGC
Cuts at: 2068 2068
Size: 6526
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5035
Fragments arranged by size:
5035 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NgoMIV G'CCGG_C
Cuts at: 2066 2066
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 2588 3884 5429 1132
Size: 97 1359 1296 1545 2229
Fragments arranged by size:
2229 1545 1359 1296 97
PasI CC'CwG_GG
Cuts at: 1348 2696 1348
Size: 1348 5178
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2362 1108
Size: 1254 5272
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2165 2158
Size: 7 6519
PshAI GACnn'nnGTC
Cuts at: 1613 2163 1613
Size: 550 5976
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PspOMI G'GGCC_C
Cuts at: 2233 2233
Size: 6526
PspXI vC'TCGA_Gb
Cuts at: 3149 3149
Size: 6526
PstI C_TGCA'G
Cuts at: 830 2184 2922 2958 3078 3878 830
Size: 1354 738 36 120 800 3478
Fragments arranged by size:
3478 1354 800 738 120 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
PvuII CAG'CTG
Cuts at: 2660 2686 2959 3199 2660
Size: 26 273 240 5987
Fragments arranged by size:
5987 273 240 26
SacI G_AGCT'C
Cuts at: 2928 3358 2928
Size: 430 6096
SacII CC_GC'GG
Cuts at: 2206 2206
Size: 6526
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SapI GCTCTTCn'nnn_
Cuts at: 3063 3063
Size: 6526
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2180 2347 2918 2954 3074 3874 5009
Size: 219 1135 167 571 36 120 800 1135
Cuts at: 5009 5690 5881 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1135 800 681 571 219 191
167 120 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 2152 3853 1872
Size: 280 1701 4545
Fragments arranged by size:
4545 1701 280
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3149 3698 4630 5531 5793 820
Size: 197 926 1206 549 932 901 262 1553
Fragments arranged by size:
1553 1206 932 926 901 549 262 197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3884 1132
Size: 2752 3774
SspI AAT'ATT
Cuts at: 5 52 4450 5
Size: 47 4398 2081
Fragments arranged by size:
4398 2081 47
StuI AGG'CCT
Cuts at: 1517 3752 1517
Size: 2235 4291
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 3965 513
Size: 552 939 477 1484 3074
Fragments arranged by size:
3074 1484 939 552 477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 2994 4553 4570 4723 4908 5353
Size: 1255 774 640 1559 17 153 185 445
Cuts at: 5353 325
Size: 1498
Fragments arranged by size:
1559 1498 1255 774 640 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3423 4772 6484
Size: 274 80 33 51 1443 1446 1349 1712
Cuts at: 6484 96
Size: 138
Fragments arranged by size:
1712 1446 1443 1349 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3487 3519 3698 3730 6486 6518 3487
Size: 32 179 32 2756 32 3495
Fragments arranged by size:
3495 2756 179 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6526
XbaI T'CTAG_A
Cuts at: 3004 3862 3004
Size: 858 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 2854 2132
Size: 722 5804
XhoI C'TCGA_G
Cuts at: 1943 3149 1943
Size: 1206 5320
XmaI C'CCGG_G
Cuts at: 1870 2150 3851 1870
Size: 280 1701 4545
Fragments arranged by size:
4545 1701 280
XmnI GAAnn'nnTTC
Cuts at: 2018 3348 3476 4655 2018
Size: 1330 128 1179 3889
Fragments arranged by size:
3889 1330 1179 128
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4334 276
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI