Restriction map for
pFN21AE4161
(Circular) (Six-base) MAPSORT of: pFN21AE4161.seq Check: 7999 from: 1 to: 6514
pFN21AE4161 6514 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3509 3509
Size: 6514
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4324 278
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3857 1937
Size: 1920 4594
Acc65I G'GTAC_C
Cuts at: 3835 3835
Size: 6514
AclI AA'CG_TT
Cuts at: 1563 4641 5014 1563
Size: 3078 373 3063
Fragments arranged by size:
3078 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3442 4577 5961 6229 1632
Size: 1810 1135 1384 268 1917
Fragments arranged by size:
1917 1810 1384 1135 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6317
AflIII A'CryG_T
Cuts at: 1203 2265 2918 3357 3627 5413 6390 1203
Size: 1062 653 439 270 1786 977 1327
Fragments arranged by size:
1786 1327 1062 977 653 439 270
AgeI A'CCGG_T
Cuts at: 5345 5345
Size: 6514
AhdI GACnn_n'nnGTC
Cuts at: 5243 5243
Size: 6514
AleI CACnn'nnGTG
Cuts at: 1166 2328 1166
Size: 1162 5352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6514
AlwNI CAG_nnn'CTG
Cuts at: 2234 2237 2597 2771 2891 3400 4257 5829 2234
Size: 3 360 174 120 509 857 1572 2919
Fragments arranged by size:
2919 1572 857 509 360 174 120 3
ApaI G_GGCC'C
Cuts at: 2462 2483 2899 3261 2462
Size: 21 416 362 5715
Fragments arranged by size:
5715 416 362 21
ApaLI G'TGCA_C
Cuts at: 2820 3496 4571 5727 2820
Size: 676 1075 1156 3607
Fragments arranged by size:
3607 1156 1075 676
ApoI r'AATT_y
Cuts at: 1493 3823 4049 4201 6310 1493
Size: 2330 226 152 2109 1697
Fragments arranged by size:
2330 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5068 160
Size: 872 4036 1606
Fragments arranged by size:
4036 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
AvaI C'yCGr_G
Cuts at: 1870 1943 2285 2759 2898 3072 3434 3839 1870
Size: 73 342 474 139 174 362 405 4545
Fragments arranged by size:
4545 474 405 362 342 174 139 73
BamHI G'GATC_C
Cuts at: 3844 4211 3844
Size: 367 6147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2057 3022 3052 3155
Size: 325 217 627 253 17 965 30 103
Cuts at: 3155 3645 3835 5290 618
Size: 490 190 1455 1842
Fragments arranged by size:
1842 1455 965 627 490 325 253 217
190 103 30 17
BanII G_rGCy'C
Cuts at: 1391 2462 2483 2817 2899 3261 3833 1391
Size: 1071 21 334 82 362 572 4072
Fragments arranged by size:
4072 1071 572 362 334 82 21
BbeI G_GCGC'C
Cuts at: 3056 3056
Size: 6514
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5543
BbvCI CC'TCA_GC
Cuts at: 2472 2488 2472
Size: 16 6498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4705 4739 1903
Size: 34 2768 34 3678
Fragments arranged by size:
3678 2768 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4406 5616 4406
Size: 1210 5304
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6514
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2457 3256 3738
Size: 107 122 71 631 605 785 799 482
Cuts at: 3738 3795 5125 136
Size: 57 1330 1525
Fragments arranged by size:
1525 1330 799 785 631 605 482 122
107 71 57
BglII A'GATC_T
Cuts at: 3453 6509 3453
Size: 3056 3458
BlpI GC'TnA_GC
Cuts at: 3931 3931
Size: 6514
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2373 2462 2483 2824 2899 3261 3323
Size: 255 328 89 21 341 75 362 62
Cuts at: 3323 3500 4575 5731 1790
Size: 177 1075 1156 2573
Fragments arranged by size:
2573 1156 1075 362 341 328 255 177
89 75 62 21
BmgBI CAC'GTC
Cuts at: 1318 2503 3106 3628 1318
Size: 1185 603 522 4204
Fragments arranged by size:
4204 1185 603 522
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 2835 3766
Size: 416 53 83 399 24 966 452 931
Cuts at: 3766 4245 5203 442
Size: 479 958 1753
Fragments arranged by size:
1753 966 958 931 479 452 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3590
Size: 6514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3221 3425 3580 5174 1239
Size: 1982 204 155 1594 2579
Fragments arranged by size:
2579 1982 1594 204 155
Bpu10I CC'TnA_GC
Cuts at: 2118 2472 2488 2118
Size: 354 16 6144
Fragments arranged by size:
6144 354 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 3476 4639 5504 5802 2084
Size: 1392 1163 865 298 2796
Fragments arranged by size:
2796 1392 1163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3561 5177 882
Size: 617 2062 1616 2219
Fragments arranged by size:
2219 2062 1616 617
BsaAI yAC'GTr
Cuts at: 493 1204 2268 3100 3358 3442 4305 493
Size: 711 1064 832 258 84 863 2702
Fragments arranged by size:
2702 1064 863 832 711 258 84
BsaBI GATnn'nnATC
Cuts at: 6508 6508
Size: 6514
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3053 4321 4703 5338
Size: 53 83 186 936 1520 1268 382 635
Cuts at: 5338 275
Size: 1451
Fragments arranged by size:
1520 1451 1268 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2660 4947 5345 5619 5766 6177 2660
Size: 2287 398 274 147 411 2997
Fragments arranged by size:
2997 2287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2398 2416 2428 2446 3167
Size: 30 165 30 995 18 12 18 721
Cuts at: 3167 3197 1178
Size: 30 4495
Fragments arranged by size:
4495 995 721 165 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2424 2489 3188 3818 1186
Size: 1024 214 65 699 630 3882
Fragments arranged by size:
3882 1024 699 630 214 65
BseYI C'CCAG_C
Cuts at: 2205 2590 2963 5717 6213 2205
Size: 385 373 2754 496 2506
Fragments arranged by size:
2754 2506 496 385 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5941
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2582 2809 3279 3715 3799 3964
Size: 275 186 581 227 470 436 84 165
Cuts at: 3964 4725 5753 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 581 470 436 275 227
186 165 84
BsiHKAI G_wGCw'C
Cuts at: 2824 3441 3500 4575 4660 5731 2824
Size: 617 59 1075 85 1071 3607
Fragments arranged by size:
3607 1075 1071 617 85 59
BsmI GAATG_Cn'
Cuts at: 4025 4118 4025
Size: 93 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2373 2462 2483 2817 2824 2899
Size: 399 255 328 89 21 334 7 75
Cuts at: 2899 3261 3323 3441 3500 3833 4575 4660 5731
Size: 362 62 118 59 333 742 85 1071
Cuts at: 5731 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 362 334 333 328
255 118 89 85 75 62 59 21
7
BspEI T'CCGG_A
Cuts at: 6177 6177
Size: 6514
BspHI T'CATG_A
Cuts at: 4403 6133 4403
Size: 1730 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 3509 3678 3869 844
Size: 1673 992 169 191 3489
Fragments arranged by size:
3489 1673 992 191 169
BsrBI CCG'CTC
Cuts at: 1394 1823 2218 2755 3574 4401 1394
Size: 429 395 537 819 827 3507
Fragments arranged by size:
3507 827 819 537 429 395
BsrDI GCAATG_nn'
Cuts at: 65 1248 3778 5009 5183 65
Size: 1183 2530 1231 174 1396
Fragments arranged by size:
2530 1396 1231 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2209 2492 2629 3351 3794 5158 5345
Size: 110 399 283 137 722 443 1364 187
Cuts at: 5345 1700
Size: 2869
Fragments arranged by size:
2869 1364 722 443 399 283 187 137
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6514
BssHII G'CGCG_C
Cuts at: 1925 3475 3477 1925
Size: 1550 2 4962
Fragments arranged by size:
4962 1550 2
BssSI C'ACGA_G
Cuts at: 4574 5586 4574
Size: 1012 5502
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2234 3600 794
Size: 1440 1366 3708
Fragments arranged by size:
3708 1440 1366
BstBI TT'CG_AA
Cuts at: 5318 5318
Size: 6514
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6514
BstXI CCAn_nnnn'nTGG
Cuts at: 2330 2333 2641 2971 2330
Size: 3 308 330 5873
Fragments arranged by size:
5873 330 308 3
BstYI r'GATC_y
Cuts at: 1972 2645 3378 3453 3844 4211 4596 4613 6065
Size: 673 733 75 391 367 385 17 1452
Cuts at: 6065 6151 6163 6509 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 733 673 391 385 367 346
86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 3036 3036
Size: 6514
BtgI C'CryG_G
Cuts at: 513 1065 2004 2011 2146 2694 3244 513
Size: 552 939 7 135 548 550 3783
Fragments arranged by size:
3783 939 552 550 548 135 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2770 3597 487
Size: 683 56 1544 827 3404
Fragments arranged by size:
3404 1544 827 683 56
BtsI GCAGTG_nn'
Cuts at: 3291 4030 4824 4844 3291
Size: 739 794 20 4961
Fragments arranged by size:
4961 794 739 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2163 2198 529
Size: 35 1599 35 4845
Fragments arranged by size:
4845 1599 35 35
DraI TTT'AAA
Cuts at: 3818 3818
Size: 6514
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5521 809
Size: 507 4205 1802
Fragments arranged by size:
4205 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2520 2631
Size: 54 1425 50 275 18 354 336 111
Cuts at: 2631 2806 2985 3276 3288 3483 3796 3961 4850
Size: 175 179 291 12 195 313 165 889
Cuts at: 4850 6479 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 354 336 313 291 275
195 179 175 165 111 54 50 43
18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2806 3276 3796 3961 1537
Size: 275 994 470 520 165 4090
Fragments arranged by size:
4090 994 520 470 275 165
EarI CTCTTCn'nnn_
Cuts at: 1353 2745 3233 4444 1353
Size: 1392 488 1211 3423
Fragments arranged by size:
3423 1392 1211 488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2419 2602 3008 3051 3341 5101
Size: 1064 138 830 183 406 43 290 1760
Cuts at: 5101 5475 5621 387
Size: 374 146 1280
Fragments arranged by size:
1760 1280 1064 830 406 374 290 183
146 138 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3221 3425 3442 3580 4577 5174 5961
Size: 393 1589 204 17 138 997 597 787
Cuts at: 5961 6229 1239
Size: 268 1524
Fragments arranged by size:
1589 1524 997 787 597 393 268 204
138 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2442 2744 2442
Size: 302 6212
EcoO109I rG'GnC_Cy
Cuts at: 2194 2458 2459 2479 2480 2737 2768 2888 2895
Size: 264 1 20 1 257 31 120 7
Cuts at: 2895 3258 2194
Size: 363 5450
Fragments arranged by size:
5450 363 264 257 120 31 20 7
1 1
EcoRI G'AATT_C
Cuts at: 3823 4201 3823
Size: 378 6136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 6514
FseI GG_CCGG'CC
Cuts at: 3798 3798
Size: 6514
FspI TGC'GCA
Cuts at: 2783 5020 2783
Size: 2237 4277
FspAI rTGC'GCAy
Cuts at: 2783 2783
Size: 6514
HaeII r_GCGC'y
Cuts at: 1678 2413 2597 3056 5661 1678
Size: 735 184 459 2605 2531
Fragments arranged by size:
2605 2531 735 459 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 2498 2530 5155
Size: 32 135 32 826 32 68 32 2625
Cuts at: 5155 5187 5229 5261 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2625 826 135 68 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3858 4104 669
Size: 988 281 1920 246 3079
Fragments arranged by size:
3079 1920 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6514
HpaI GTT'AAC
Cuts at: 4104 4104
Size: 6514
KasI G'GCGC_C
Cuts at: 3052 3052
Size: 6514
KpnI G_GTAC'C
Cuts at: 3839 3839
Size: 6514
MfeI C'AATT_G
Cuts at: 4113 4113
Size: 6514
MluI A'CGCG_T
Cuts at: 6390 6390
Size: 6514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 5628 5812 1305
Size: 1521 2802 184 2007
Fragments arranged by size:
2802 2007 1521 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 2987 10
Size: 54 1425 343 354 801 3537
Fragments arranged by size:
3537 1425 801 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 4472 4831 4990 518
Size: 648 1162 2144 359 159 2042
Fragments arranged by size:
2144 2042 1162 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2234 2548 2832 3246 3397 3642 3930 4607
Size: 628 314 284 414 151 245 288 677
Cuts at: 4607 5755 6000 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 628 414 314 288 284
245 245 151
NaeI GCC'GGC
Cuts at: 2211 3796 2211
Size: 1585 4929
NarI GG'CG_CC
Cuts at: 3053 3053
Size: 6514
NcoI C'CATG_G
Cuts at: 513 1065 2004 2146 513
Size: 552 939 142 4881
Fragments arranged by size:
4881 939 552 142
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6514
NgoMIV G'CCGG_C
Cuts at: 2209 3794 2209
Size: 1585 4929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6514
NotI GC'GGCC_GC
Cuts at: 3961 3961
Size: 6514
NspI r_CATG'y
Cuts at: 1132 1229 2922 3424 3872 5417 1132
Size: 97 1693 502 448 1545 2229
Fragments arranged by size:
2229 1693 1545 502 448 97
PasI CC'CwG_GG
Cuts at: 1348 2444 2771 1348
Size: 1096 327 5091
Fragments arranged by size:
5091 1096 327
PciI A'CATG_T
Cuts at: 2918 5413 2918
Size: 2495 4019
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2147 2771 2891 1108
Size: 1039 624 120 4731
Fragments arranged by size:
4731 1039 624 120
PfoI T'CCnGG_A
Cuts at: 2259 2259
Size: 6514
PmeI GTTT'AAAC
Cuts at: 3818 3818
Size: 6514
PmlI CAC'GTG
Cuts at: 2268 3100 3358 3442 2268
Size: 832 258 84 5340
Fragments arranged by size:
5340 832 258 84
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4583 4615 6096 6128 4583
Size: 32 1481 32 4969
Fragments arranged by size:
4969 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2888 2194
Size: 694 5820
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6514
PsiI TTA'TAA
Cuts at: 4084 4084
Size: 6514
PspOMI G'GGCC_C
Cuts at: 2458 2479 2895 3257 2458
Size: 21 416 362 5715
Fragments arranged by size:
5715 416 362 21
PstI C_TGCA'G
Cuts at: 830 2568 2678 3866 830
Size: 1738 110 1188 3478
Fragments arranged by size:
3478 1738 1188 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6514
PvuII CAG'CTG
Cuts at: 2234 2234
Size: 6514
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 6514
SacII CC_GC'GG
Cuts at: 3247 3247
Size: 6514
SalI G'TCGA_C
Cuts at: 1936 3856 1936
Size: 1920 4594
SapI GCTCTTCn'nnn_
Cuts at: 2745 3233 2745
Size: 488 6026
SbfI CC_TGCA'GG
Cuts at: 3866 3866
Size: 6514
ScaI AGT'ACT
Cuts at: 4762 6474 4762
Size: 1712 4802
SfcI C'TryA_G
Cuts at: 826 1045 2564 2598 2674 3862 4997 5678 5869
Size: 219 1519 34 76 1188 1135 681 191
Cuts at: 5869 826
Size: 1471
Fragments arranged by size:
1519 1471 1188 1135 681 219 191 76
34
SfiI GGCCn_nnn'nGGCC
Cuts at: 2457 3256 3795 2457
Size: 799 539 5176
Fragments arranged by size:
5176 799 539
SfoI GGC'GCC
Cuts at: 3054 3054
Size: 6514
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6514
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6514
SmaI CCC'GGG
Cuts at: 1872 2287 2761 3074 3841 1872
Size: 415 474 313 767 4545
Fragments arranged by size:
4545 767 474 415 313
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 3491 4618 5519 5781 820
Size: 197 926 156 1392 1127 901 262 1553
Fragments arranged by size:
1553 1392 1127 926 901 262 197 156
SnaBI TAC'GTA
Cuts at: 493 4305 493
Size: 3812 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6514
SphI G_CATG'C
Cuts at: 1132 3872 1132
Size: 2740 3774
SrfI GCCC'GGGC
Cuts at: 2761 2761
Size: 6514
SspI AAT'ATT
Cuts at: 5 52 4438 5
Size: 47 4386 2081
Fragments arranged by size:
4386 2081 47
StuI AGG'CCT
Cuts at: 1517 2860 3034 1517
Size: 1343 174 4997
Fragments arranged by size:
4997 1343 174
StyI C'CwwG_G
Cuts at: 513 1065 2004 2146 2181 2391 2427 2634 2733
Size: 552 939 142 35 210 36 207 99
Cuts at: 2733 2904 3953 513
Size: 171 1049 3074
Fragments arranged by size:
3074 1049 939 552 210 207 171 142
99 36 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2596 3748 4541 4558 4711 4896 5341
Size: 1255 1016 1152 793 17 153 185 445
Cuts at: 5341 325
Size: 1498
Fragments arranged by size:
1498 1255 1152 1016 793 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3417 4760 6472
Size: 274 80 33 51 1443 1440 1343 1712
Cuts at: 6472 96
Size: 138
Fragments arranged by size:
1712 1443 1440 1343 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2416 2448 3167 3199 6474 6506 2416
Size: 32 719 32 3275 32 2424
Fragments arranged by size:
3275 2424 719 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6514
XbaI T'CTAG_A
Cuts at: 3850 3850
Size: 6514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 3478 2330
Size: 1148 5366
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6514
XmaI C'CCGG_G
Cuts at: 1870 2285 2759 3072 3839 1870
Size: 415 474 313 767 4545
Fragments arranged by size:
4545 767 474 415 313
XmnI GAAnn'nnTTC
Cuts at: 4643 4643
Size: 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4322 276
Size: 53 83 186 936 2788 2468
Fragments arranged by size:
2788 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV NruI NsiI PacI PspXI
PsrI SacI SanDI SexAI SwaI