Restriction map for
pFN21AE4159
(Circular) (Six-base) MAPSORT of: pFN21AE4159.seq Check: 4284 from: 1 to: 6505
pFN21AE4159 6505 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4315 278
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3848 1937
Size: 1911 4594
Acc65I G'GTAC_C
Cuts at: 2874 3826 2874
Size: 952 5553
AclI AA'CG_TT
Cuts at: 1563 4632 5005 1563
Size: 3069 373 3063
Fragments arranged by size:
3069 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2118 2260 2367 3330 4568 5952 6220 1632
Size: 486 142 107 963 1238 1384 268 1917
Fragments arranged by size:
1917 1384 1238 963 486 268 142 107
AfeI AGC'GCT
Cuts at: 2522 2522
Size: 6505
AflII C'TTAA_G
Cuts at: 820 1017 3323 820
Size: 197 2306 4002
Fragments arranged by size:
4002 2306 197
AflIII A'CryG_T
Cuts at: 1203 2764 5404 6381 1203
Size: 1561 2640 977 1327
Fragments arranged by size:
2640 1561 1327 977
AgeI A'CCGG_T
Cuts at: 5336 5336
Size: 6505
AhdI GACnn_n'nnGTC
Cuts at: 5234 5234
Size: 6505
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6505
AlwNI CAG_nnn'CTG
Cuts at: 4248 5820 4248
Size: 1572 4933
ApaLI G'TGCA_C
Cuts at: 4562 5718 4562
Size: 1156 5349
ApoI r'AATT_y
Cuts at: 1493 2177 2231 2569 2612 2833 3053 3814 4040
Size: 684 54 338 43 221 220 761 226
Cuts at: 4040 4192 6301 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 761 684 338 226 221 220
152 54 43
AseI AT'TA_AT
Cuts at: 160 1032 5059 160
Size: 872 4027 1606
Fragments arranged by size:
4027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
AvaI C'yCGr_G
Cuts at: 1870 1943 3830 1870
Size: 73 1887 4545
Fragments arranged by size:
4545 1887 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3480 3513 3480
Size: 33 6472
BamHI G'GATC_C
Cuts at: 3835 4202 3835
Size: 367 6138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2874 3826 5281 618
Size: 325 217 627 1087 952 1455 1842
Fragments arranged by size:
1842 1455 1087 952 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2757 3824 1391
Size: 1366 1067 4072
Fragments arranged by size:
4072 1366 1067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2743 3664 928
Size: 971 844 921 3769
Fragments arranged by size:
3769 971 921 844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4696 4730 1903
Size: 34 2759 34 3678
Fragments arranged by size:
3678 2759 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2608 3508 4397 5607 2608
Size: 900 889 1210 3506
Fragments arranged by size:
3506 1210 900 889
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6505
BfrBI ATG'CAT
Cuts at: 2465 2465
Size: 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5116 136
Size: 107 122 71 631 605 3444 1525
Fragments arranged by size:
3444 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6500 6500
Size: 6505
BlpI GC'TnA_GC
Cuts at: 3042 3922 3042
Size: 880 5625
Bme1580I G_kGCm'C
Cuts at: 1790 4566 5722 1790
Size: 2776 1156 2573
Fragments arranged by size:
2776 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6505
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4236 5194 442
Size: 416 53 83 399 24 2819 958 1753
Fragments arranged by size:
2819 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2793 2270
Size: 523 5982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 5165 1239
Size: 908 3018 2579
Fragments arranged by size:
3018 2579 908
Bpu10I CC'TnA_GC
Cuts at: 2509 2509
Size: 6505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 4630 5495 5793 2639
Size: 1991 865 298 3351
Fragments arranged by size:
3351 1991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5168 882
Size: 617 3669 2219
Fragments arranged by size:
3669 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3583 4296 493
Size: 711 2379 713 2702
Fragments arranged by size:
2702 2379 713 711
BsaBI GATnn'nnATC
Cuts at: 6499 6499
Size: 6505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3199 4312 4694 5329
Size: 53 83 186 936 1666 1113 382 635
Cuts at: 5329 275
Size: 1451
Fragments arranged by size:
1666 1451 1113 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4938 5336 5610 5757 6168 4938
Size: 398 274 147 411 5275
Fragments arranged by size:
5275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2739 2769 1178
Size: 30 165 30 1336 30 4914
Fragments arranged by size:
4914 1336 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 2696 1186
Size: 1312 198 4995
Fragments arranged by size:
4995 1312 198
BseYI C'CCAG_C
Cuts at: 2062 2601 5708 6204 2062
Size: 539 3107 496 2363
Fragments arranged by size:
3107 2363 539 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3480 1163
Size: 573 1744 4188
Fragments arranged by size:
4188 1744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3955 4716 5744 1540
Size: 275 186 1954 761 1028 2301
Fragments arranged by size:
2301 1954 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2757 3085 4566 4651 5722 2256
Size: 501 328 1481 85 1071 3039
Fragments arranged by size:
3039 1481 1071 501 328 85
BsmI GAATG_Cn'
Cuts at: 4016 4109 4016
Size: 93 6412
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2256 2757 3085 3824 4566 4651 5722
Size: 399 466 501 328 739 742 85 1071
Cuts at: 5722 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 739 501 466 399 328
85
BspEI T'CCGG_A
Cuts at: 6168 6168
Size: 6505
BspHI T'CATG_A
Cuts at: 2113 4394 6124 2113
Size: 2281 1730 2494
Fragments arranged by size:
2494 2281 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2913 3860 844
Size: 2069 947 3489
Fragments arranged by size:
3489 2069 947
BsrBI CCG'CTC
Cuts at: 1394 1823 4392 1394
Size: 429 2569 3507
Fragments arranged by size:
3507 2569 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3741 5000 5174 65
Size: 1183 2493 1259 174 1396
Fragments arranged by size:
2493 1396 1259 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3524 5149 5336 1700
Size: 110 1714 1625 187 2869
Fragments arranged by size:
2869 1714 1625 187 110
BsrGI T'GTAC_A
Cuts at: 96 2410 96
Size: 2314 4191
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6505
BssSI C'ACGA_G
Cuts at: 2750 4565 5577 2750
Size: 1815 1012 3678
Fragments arranged by size:
3678 1815 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3080 794
Size: 2286 4219
BstBI TT'CG_AA
Cuts at: 5309 5309
Size: 6505
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6505
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 2885 2479
Size: 406 6099
BstYI r'GATC_y
Cuts at: 1972 2041 3835 4202 4587 4604 6056 6142 6154
Size: 69 1794 367 385 17 1452 86 12
Cuts at: 6154 6500 1972
Size: 346 1977
Fragments arranged by size:
1977 1794 1452 385 367 346 86 69
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5014
Fragments arranged by size:
5014 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5766
Fragments arranged by size:
5766 683 56
BtsI GCAGTG_nn'
Cuts at: 3354 4021 4815 4835 3354
Size: 667 794 20 5024
Fragments arranged by size:
5024 794 667 20
ClaI AT'CG_AT
Cuts at: 1554 3301 1554
Size: 1747 4758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6470
DraI TTT'AAA
Cuts at: 3799 3809 3799
Size: 10 6495
DraIII CAC_nnn'GTG
Cuts at: 3003 3003
Size: 6505
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5512 809
Size: 507 4196 1802
Fragments arranged by size:
4196 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3952 4841 6470
Size: 54 1425 50 275 18 2122 889 1629
Cuts at: 6470 8
Size: 43
Fragments arranged by size:
2122 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3952 1537
Size: 275 2140 4090
Fragments arranged by size:
4090 2140 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2137 2558 4435 1353
Size: 784 421 1877 3423
Fragments arranged by size:
3423 1877 784 421
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3669 5092 5466 5612 387
Size: 1064 138 2080 1423 374 146 1280
Fragments arranged by size:
2080 1423 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2755 2755
Size: 6505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2147 2260 2367 3330 4568 5165
Size: 393 486 29 113 107 963 1238 597
Cuts at: 5165 5952 6220 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1238 963 787 597 486 393 268
113 107 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3018 3018
Size: 6505
EcoO109I rG'GnC_Cy
Cuts at: 3389 3389
Size: 6505
EcoRI G'AATT_C
Cuts at: 2833 3814 4192 2833
Size: 981 378 5146
Fragments arranged by size:
5146 981 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2633 2654 2633
Size: 21 6484
FspI TGC'GCA
Cuts at: 5011 5011
Size: 6505
HaeII r_GCGC'y
Cuts at: 1678 2524 5652 1678
Size: 846 3128 2531
Fragments arranged by size:
3128 2531 846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2582 2614 5146
Size: 32 135 32 512 32 466 32 2532
Cuts at: 5146 5178 5220 5252 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2532 512 466 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3849 4095 669
Size: 988 281 1911 246 3079
Fragments arranged by size:
3079 1911 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3044 748
Size: 2296 4209
HpaI GTT'AAC
Cuts at: 4095 4095
Size: 6505
KpnI G_GTAC'C
Cuts at: 2878 3830 2878
Size: 952 5553
MfeI C'AATT_G
Cuts at: 2822 3471 4104 2822
Size: 649 633 5223
Fragments arranged by size:
5223 649 633
MluI A'CGCG_T
Cuts at: 6381 6381
Size: 6505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2938 2956 5619 5803 1305
Size: 1633 18 2663 184 2007
Fragments arranged by size:
2663 2007 1633 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4683
Fragments arranged by size:
4683 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 3762 4463 4822 4981 518
Size: 648 1050 1546 701 359 159 2042
Fragments arranged by size:
2042 1546 1050 701 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3494 3921 4598 5746 5991 1606
Size: 1888 427 677 1148 245 2120
Fragments arranged by size:
2120 1888 1148 677 427 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5014
Fragments arranged by size:
5014 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6505
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6505
NotI GC'GGCC_GC
Cuts at: 3952 3952
Size: 6505
NsiI A_TGCA'T
Cuts at: 2467 2467
Size: 6505
NspI r_CATG'y
Cuts at: 1132 1229 2469 2768 3863 5408 1132
Size: 97 1240 299 1095 1545 2229
Fragments arranged by size:
2229 1545 1240 1095 299 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6505
PciI A'CATG_T
Cuts at: 2764 5404 2764
Size: 2640 3865
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3447 1108
Size: 2339 4166
PmeI GTTT'AAAC
Cuts at: 3809 3809
Size: 6505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 6087 6119 4574
Size: 32 1481 32 4960
Fragments arranged by size:
4960 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3389 3389
Size: 6505
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6505
PsiI TTA'TAA
Cuts at: 3144 4075 3144
Size: 931 5574
PstI C_TGCA'G
Cuts at: 830 3349 3857 830
Size: 2519 508 3478
Fragments arranged by size:
3478 2519 508
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6505
PvuII CAG'CTG
Cuts at: 3494 3494
Size: 6505
SacI G_AGCT'C
Cuts at: 2757 2757
Size: 6505
SalI G'TCGA_C
Cuts at: 1936 3847 1936
Size: 1911 4594
SbfI CC_TGCA'GG
Cuts at: 3857 3857
Size: 6505
ScaI AGT'ACT
Cuts at: 4753 6465 4753
Size: 1712 4793
SfcI C'TryA_G
Cuts at: 826 1045 2263 2400 3345 3853 4988 5669 5860
Size: 219 1218 137 945 508 1135 681 191
Cuts at: 5860 826
Size: 1471
Fragments arranged by size:
1471 1218 1135 945 681 508 219 191
137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6505
SmaI CCC'GGG
Cuts at: 1872 3832 1872
Size: 1960 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3323 4609 5510 5772 820
Size: 197 926 675 705 1286 901 262 1553
Fragments arranged by size:
1553 1286 926 901 705 675 262 197
SnaBI TAC'GTA
Cuts at: 493 4296 493
Size: 3803 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6505
SphI G_CATG'C
Cuts at: 1132 3863 1132
Size: 2731 3774
SspI AAT'ATT
Cuts at: 5 52 2016 3160 4429 5
Size: 47 1964 1144 1269 2081
Fragments arranged by size:
2081 1964 1269 1144 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6505
StyI C'CwwG_G
Cuts at: 513 1065 2004 2906 3220 3537 3740 3944 513
Size: 552 939 902 314 317 203 204 3074
Fragments arranged by size:
3074 939 902 552 317 314 204 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4532 4549 4702 4887 5332 325
Size: 1255 2952 17 153 185 445 1498
Fragments arranged by size:
2952 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2410 4751 6463
Size: 274 80 33 51 1443 433 2341 1712
Cuts at: 6463 96
Size: 138
Fragments arranged by size:
2341 1712 1443 433 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6465 6497 6465
Size: 32 6473
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6505
XbaI T'CTAG_A
Cuts at: 3841 3841
Size: 6505
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6505
XmaI C'CCGG_G
Cuts at: 1870 3830 1870
Size: 1960 4545
XmnI GAAnn'nnTTC
Cuts at: 2613 3593 4634 2613
Size: 980 1041 4484
Fragments arranged by size:
4484 1041 980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4313 276
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI