Restriction map for
pFN21AE4153
(Circular) (Six-base) MAPSORT of: pFN21AE4153.seq Check: 6240 from: 1 to: 6469
pFN21AE4153 6469 bp
With 182 enzymes: SgfI *
March 28, 2012 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3110 3110
Size: 6469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2486 3154 3812 1937
Size: 549 668 658 4594
Fragments arranged by size:
4594 668 658 549
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3566 4532 5916 6184 1632
Size: 1934 966 1384 268 1917
Fragments arranged by size:
1934 1917 1384 966 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 2676 5368 6345 1203
Size: 1473 2692 977 1327
Fragments arranged by size:
2692 1473 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 2482 5198 2482
Size: 2716 3753
AleI CACnn'nnGTG
Cuts at: 1166 2700 1166
Size: 1534 4935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2889 1184
Size: 1705 4764
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2676 2708 2676
Size: 32 6437
AlwNI CAG_nnn'CTG
Cuts at: 4212 5784 4212
Size: 1572 4897
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 2318 3370 3636 3778 4004 4156 6265 1493
Size: 825 1052 266 142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1052 825 266 226 152 142
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 3794 1870
Size: 73 1851 4545
Fragments arranged by size:
4545 1851 73
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3790 5245 618
Size: 325 217 627 2003 1455 1842
Fragments arranged by size:
2003 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2471 3788 1391
Size: 790 290 1317 4072
Fragments arranged by size:
4072 1317 790 290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 3062 928
Size: 971 138 1025 4335
Fragments arranged by size:
4335 1025 971 138
BbvCI CC'TCA_GC
Cuts at: 3299 3299
Size: 6469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3608 3642 4660 4694 1903
Size: 34 1671 34 1018 34 3678
Fragments arranged by size:
3678 1671 1018 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 4361 5571 2002
Size: 2359 1210 2900
Fragments arranged by size:
2900 2359 1210
BclI T'GATC_A
Cuts at: 1548 2493 1548
Size: 945 5524
BfrBI ATG'CAT
Cuts at: 2297 2548 3238 3352 2297
Size: 251 690 114 5414
Fragments arranged by size:
5414 690 251 114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5080 136
Size: 107 122 71 631 605 3408 1525
Fragments arranged by size:
3408 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 4530 5686 1790
Size: 2740 1156 2573
Fragments arranged by size:
2740 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2677 1318
Size: 1359 5110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2888 3255 4200
Size: 416 53 83 399 24 1471 367 945
Cuts at: 4200 5158 442
Size: 958 1753
Fragments arranged by size:
1753 1471 958 945 416 399 367 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 3641 5129 1239
Size: 926 1476 1488 2579
Fragments arranged by size:
2579 1488 1476 926
Bpu10I CC'TnA_GC
Cuts at: 3299 3299
Size: 6469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2300 4594 5459 5757 2195
Size: 105 2294 865 298 2907
Fragments arranged by size:
2907 2294 865 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2432 3659 5132 882
Size: 617 933 1227 1473 2219
Fragments arranged by size:
2219 1473 1227 933 617
BsaAI yAC'GTr
Cuts at: 493 1204 4260 493
Size: 711 3056 2702
Fragments arranged by size:
3056 2702 711
BsaBI GATnn'nnATC
Cuts at: 3418 6463 3418
Size: 3045 3424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2649 2735 4276 4658
Size: 53 83 186 936 1116 86 1541 382
Cuts at: 4658 5293 275
Size: 635 1451
Fragments arranged by size:
1541 1451 1116 936 635 382 186 86
83 53
BsaWI w'CCGG_w
Cuts at: 4902 5300 5574 5721 6132 4902
Size: 398 274 147 411 5239
Fragments arranged by size:
5239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2595 2625 3204 3234 1178
Size: 30 165 30 1192 30 579 30 4413
Fragments arranged by size:
4413 1192 579 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6469
BseYI C'CCAG_C
Cuts at: 5672 6168 5672
Size: 496 5973
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 3144 3537 1163
Size: 573 343 1065 393 4095
Fragments arranged by size:
4095 1065 573 393 343
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2131 2181 4530 4615 5686 2131
Size: 50 2349 85 1071 2914
Fragments arranged by size:
2914 2349 1071 85 50
BsmI GAATG_Cn'
Cuts at: 3980 4073 3980
Size: 93 6376
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2181 2471 3788 4530 4615 5686
Size: 399 341 50 290 1317 742 85 1071
Cuts at: 5686 1391
Size: 2174
Fragments arranged by size:
2174 1317 1071 742 399 341 290 85
50
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 3824 844
Size: 2266 714 3489
Fragments arranged by size:
3489 2266 714
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2214 2240 2550 2614 3094 4964 5138
Size: 1183 966 26 310 64 480 1870 174
Cuts at: 5138 65
Size: 1396
Fragments arranged by size:
1870 1396 1183 966 480 310 174 64
26
BsrFI r'CCGG_y
Cuts at: 1700 1810 5113 5300 1700
Size: 110 3303 187 2869
Fragments arranged by size:
3303 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 3498 4529 5541 3498
Size: 1031 1012 4426
Fragments arranged by size:
4426 1031 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6469
BstBI TT'CG_AA
Cuts at: 3640 5273 3640
Size: 1633 4836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstXI CCAn_nnnn'nTGG
Cuts at: 2702 2702
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 3139 3799 4166 4551 4568 6020 6106 6118
Size: 1167 660 367 385 17 1452 86 12
Cuts at: 6118 6464 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1167 660 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3208 513
Size: 552 939 1204 3774
Fragments arranged by size:
3774 1204 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2548 487
Size: 683 56 1322 4408
Fragments arranged by size:
4408 1322 683 56
BtsI GCAGTG_nn'
Cuts at: 3171 3985 4779 4799 3171
Size: 814 794 20 4841
Fragments arranged by size:
4841 814 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 3730 3773 3730
Size: 43 6426
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3575 3916 4805
Size: 54 1425 50 275 18 1745 341 889
Cuts at: 4805 6434 8
Size: 1629 43
Fragments arranged by size:
1745 1629 1425 889 341 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3393 4399 1353
Size: 2040 1006 3423
Fragments arranged by size:
3423 2040 1006
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2738 3575 5056 5430 5576 387
Size: 1064 138 1149 837 1481 374 146 1280
Fragments arranged by size:
1481 1280 1149 1064 837 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 2179
Size: 6469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 3566 3641 4532 5129 5916 6184
Size: 393 533 1401 75 891 597 787 268
Cuts at: 6184 1239
Size: 1524
Fragments arranged by size:
1524 1401 891 787 597 533 393 268
75
EcoRI G'AATT_C
Cuts at: 2318 3778 4156 2318
Size: 1460 378 4631
Fragments arranged by size:
4631 1460 378
EcoRV GAT'ATC
Cuts at: 2578 2578
Size: 6469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2054 2054
Size: 6469
FspI TGC'GCA
Cuts at: 4975 4975
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 5616 1678
Size: 3938 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2595 2619 2627
Size: 32 135 32 896 32 95 24 8
Cuts at: 2627 2651 2720 2752 3204 3236 5110 5142 5184
Size: 24 69 32 452 32 1874 32 42
Cuts at: 5184 5216 1373
Size: 32 2626
Fragments arranged by size:
2626 1874 896 452 135 95 69 42
32 32 32 32 32 32 32 24
24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2487 3007 3813 4059 669
Size: 988 281 549 520 806 246 3079
Fragments arranged by size:
3079 988 806 549 520 281 246
HindIII A'AGCT_T
Cuts at: 748 3689 748
Size: 2941 3528
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 3223 4068 3223
Size: 845 5624
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5583 5767 1305
Size: 4278 184 2007
Fragments arranged by size:
4278 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3577 10
Size: 54 1425 343 1745 2902
Fragments arranged by size:
2902 1745 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2700 3243 3379 4427 4786 4945 518
Size: 648 1534 543 136 1048 359 159 2042
Fragments arranged by size:
2042 1534 1048 648 543 359 159 136
MspA1I CmG'CkG
Cuts at: 1606 3885 4562 5710 5955 1606
Size: 2279 677 1148 245 2120
Fragments arranged by size:
2279 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3208 513
Size: 552 939 1204 3774
Fragments arranged by size:
3774 1204 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NsiI A_TGCA'T
Cuts at: 2299 2550 3240 3354 2299
Size: 251 690 114 5414
Fragments arranged by size:
5414 690 251 114
NspI r_CATG'y
Cuts at: 1132 1229 3242 3352 3827 5372 1132
Size: 97 2013 110 475 1545 2229
Fragments arranged by size:
2229 2013 1545 475 110 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PfoI T'CCnGG_A
Cuts at: 2181 2181
Size: 6469
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PstI C_TGCA'G
Cuts at: 830 3121 3821 830
Size: 2291 700 3478
Fragments arranged by size:
3478 2291 700
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
SacI G_AGCT'C
Cuts at: 2181 2181
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 2485 3811 1936
Size: 549 1326 4594
Fragments arranged by size:
4594 1326 549
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 4717 6429 4717
Size: 1712 4757
SfcI C'TryA_G
Cuts at: 826 1045 3117 3817 4952 5633 5824 826
Size: 219 2072 700 1135 681 191 1471
Fragments arranged by size:
2072 1471 1135 700 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 2279 4573 5474 5736 820
Size: 197 926 231 105 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 231 197 105
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3352 3827 1132
Size: 2220 475 3774
Fragments arranged by size:
3774 2220 475
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 2004 3208 3467 3908 513
Size: 552 939 1204 259 441 3074
Fragments arranged by size:
3074 1204 939 552 441 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4496 4513 4666 4851 5296 325
Size: 1255 2916 17 153 185 445 1498
Fragments arranged by size:
2916 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3502 3562 4715
Size: 274 80 33 51 1443 1525 60 1153
Cuts at: 4715 6427 96
Size: 1712 138
Fragments arranged by size:
1712 1525 1443 1153 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3409 3441 6429 6461 3409
Size: 32 2988 32 3417
Fragments arranged by size:
3417 2988 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 2974 3805 2974
Size: 831 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2621 2621
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6469
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 3108 4598 3108
Size: 1490 4979
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI