Restriction map for
pFN21AE4152
(Circular) (Six-base) MAPSORT of: pFN21AE4152.seq Check: 8887 from: 1 to: 6466
pFN21AE4152 6466 bp
With 182 enzymes: SgfI *
March 28, 2012 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2862 2862
Size: 6466
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4276 278
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3809 1937
Size: 1872 4594
Acc65I G'GTAC_C
Cuts at: 3787 3787
Size: 6466
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 3099 4529 5913 6181 1632
Size: 669 798 1430 1384 268 1917
Fragments arranged by size:
1917 1430 1384 798 669 268
AfeI AGC'GCT
Cuts at: 2146 2314 2146
Size: 168 6298
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6269
AflIII A'CryG_T
Cuts at: 1203 5365 6342 1203
Size: 4162 977 1327
Fragments arranged by size:
4162 1327 977
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 2352 1166
Size: 1186 5280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2286 1184
Size: 1102 5364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2988 3020 3471 3503 2988
Size: 32 451 32 5951
Fragments arranged by size:
5951 451 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2795 2992 4209 5781 2795
Size: 197 1217 1572 3480
Fragments arranged by size:
3480 1572 1217 197
ApaLI G'TGCA_C
Cuts at: 4523 5679 4523
Size: 1156 5310
ApoI r'AATT_y
Cuts at: 1493 2292 2443 2842 3747 3775 4001 4153 6262
Size: 799 151 399 905 28 226 152 2109
Cuts at: 6262 1493
Size: 1697
Fragments arranged by size:
2109 1697 905 799 399 226 152 151
28
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 3791 1870
Size: 73 1848 4545
Fragments arranged by size:
4545 1848 73
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3787 5242 618
Size: 325 217 627 2000 1455 1842
Fragments arranged by size:
2000 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3145 3785 1391
Size: 1754 640 4072
Fragments arranged by size:
4072 1754 640
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 2046 3661 928
Size: 971 135 12 1615 3733
Fragments arranged by size:
3733 1615 971 135 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4657 4691 1903
Size: 34 2720 34 3678
Fragments arranged by size:
3678 2720 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4358 5568 4358
Size: 1210 5256
BclI T'GATC_A
Cuts at: 1548 2511 3207 3511 1548
Size: 963 696 304 4503
Fragments arranged by size:
4503 963 696 304
BfrBI ATG'CAT
Cuts at: 2195 3639 2195
Size: 1444 5022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5077 136
Size: 107 122 71 631 605 3405 1525
Fragments arranged by size:
3405 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6461 6461
Size: 6466
BlpI GC'TnA_GC
Cuts at: 3883 3883
Size: 6466
Bme1580I G_kGCm'C
Cuts at: 1790 2626 4527 5683 1790
Size: 836 1901 1156 2573
Fragments arranged by size:
2573 1901 1156 836
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2319 2591 4197
Size: 416 53 83 399 24 902 272 1606
Cuts at: 4197 5155 442
Size: 958 1753
Fragments arranged by size:
1753 1606 958 902 416 399 272 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2577 2577
Size: 6466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2831 3158 3159 5126 1239
Size: 1592 327 1 1967 2579
Fragments arranged by size:
2579 1967 1592 327 1
Bpu10I CC'TnA_GC
Cuts at: 2309 3221 3314 2309
Size: 912 93 5461
Fragments arranged by size:
5461 912 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4591 5456 5754 4591
Size: 865 298 5303
Fragments arranged by size:
5303 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5129 882
Size: 617 3630 2219
Fragments arranged by size:
3630 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2646 4257 493
Size: 711 1442 1611 2702
Fragments arranged by size:
2702 1611 1442 711
BsaBI GATnn'nnATC
Cuts at: 2532 6460 2532
Size: 3928 2538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4273 4655 5290 275
Size: 53 83 186 936 2740 382 635 1451
Fragments arranged by size:
2740 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3630 4899 5297 5571 5718 6129 3630
Size: 1269 398 274 147 411 3967
Fragments arranged by size:
3967 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2514 2544 3363 3393 3584
Size: 30 165 30 1111 30 819 30 191
Cuts at: 3584 3614 1178
Size: 30 4030
Fragments arranged by size:
4030 1111 819 191 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2451 2649 2990 1186
Size: 1265 198 341 4662
Fragments arranged by size:
4662 1265 341 198
BseYI C'CCAG_C
Cuts at: 2052 5669 6165 2052
Size: 3617 496 2353
Fragments arranged by size:
3617 2353 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2985 1163
Size: 573 1249 4644
Fragments arranged by size:
4644 1249 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3916 4677 5705 1540
Size: 275 186 1915 761 1028 2301
Fragments arranged by size:
2301 1915 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2645 2980 3145 3549 4527 4612 5683 2645
Size: 335 165 404 978 85 1071 3428
Fragments arranged by size:
3428 1071 978 404 335 165 85
BsmI GAATG_Cn'
Cuts at: 2131 3977 4070 2131
Size: 1846 93 4527
Fragments arranged by size:
4527 1846 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2626 2645 2980 3145 3549 3785 4527
Size: 399 836 19 335 165 404 236 742
Cuts at: 4527 4612 5683 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 836 742 404 399 335 236
165 85 19
BspEI T'CCGG_A
Cuts at: 3630 6129 3630
Size: 2499 3967
BspHI T'CATG_A
Cuts at: 4355 6085 4355
Size: 1730 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2862 3821 844
Size: 2018 959 3489
Fragments arranged by size:
3489 2018 959
BsrBI CCG'CTC
Cuts at: 1394 1823 4353 1394
Size: 429 2530 3507
Fragments arranged by size:
3507 2530 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4961 5135 65
Size: 1183 3713 174 1396
Fragments arranged by size:
3713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5110 5297 1700
Size: 110 3300 187 2869
Fragments arranged by size:
3300 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6466
BssSI C'ACGA_G
Cuts at: 4526 5538 4526
Size: 1012 5454
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2108 794
Size: 1314 5152
BstBI TT'CG_AA
Cuts at: 2448 5270 2448
Size: 2822 3644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6466
BstXI CCAn_nnnn'nTGG
Cuts at: 2866 2866
Size: 6466
BstYI r'GATC_y
Cuts at: 1972 2920 3522 3561 3627 3796 4163 4548 4565
Size: 948 602 39 66 169 367 385 17
Cuts at: 4565 6017 6103 6115 6461 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 948 602 385 367 346 169
86 66 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2115 3185 2115
Size: 1070 5396
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4975
Fragments arranged by size:
4975 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2037 3364 487
Size: 683 56 811 1327 3589
Fragments arranged by size:
3589 1327 811 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 3281 3982 4776 4796 2280
Size: 1001 701 794 20 3950
Fragments arranged by size:
3950 1001 794 701 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2352 2387 529
Size: 35 1788 35 4608
Fragments arranged by size:
4608 1788 35 35
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5473 809
Size: 507 4157 1802
Fragments arranged by size:
4157 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3913 4802 6431
Size: 54 1425 50 275 18 2083 889 1629
Cuts at: 6431 8
Size: 43
Fragments arranged by size:
2083 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3913 1537
Size: 275 2101 4090
Fragments arranged by size:
4090 2101 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 3118 4396 1353
Size: 1116 649 1278 3423
Fragments arranged by size:
3423 1278 1116 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5053 5427 5573 387
Size: 1064 138 3464 374 146 1280
Fragments arranged by size:
3464 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3143 3143
Size: 6466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2831 3099 3158 3159 4529 5126
Size: 393 669 530 268 59 1 1370 597
Cuts at: 5126 5913 6181 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1370 787 669 597 530 393 268
268 59 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3005 3005
Size: 6466
EcoRI G'AATT_C
Cuts at: 2842 3775 4153 2842
Size: 933 378 5155
Fragments arranged by size:
5155 933 378
FspI TGC'GCA
Cuts at: 4972 4972
Size: 6466
HaeII r_GCGC'y
Cuts at: 1678 2148 2316 5613 1678
Size: 470 168 3297 2531
Fragments arranged by size:
3297 2531 470 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2514 2546 3363
Size: 32 135 32 611 32 299 32 817
Cuts at: 3363 3395 5107 5139 5181 5213 1373
Size: 32 1712 32 42 32 2626
Fragments arranged by size:
2626 1712 817 611 299 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3166 3259 3810 4056 669
Size: 988 281 1228 93 551 246 3079
Fragments arranged by size:
3079 1228 988 551 281 246 93
HindIII A'AGCT_T
Cuts at: 748 2603 748
Size: 1855 4611
HpaI GTT'AAC
Cuts at: 3166 3259 4056 3166
Size: 93 797 5576
Fragments arranged by size:
5576 797 93
KpnI G_GTAC'C
Cuts at: 3791 3791
Size: 6466
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5580 5764 1305
Size: 4275 184 2007
Fragments arranged by size:
4275 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4644
Fragments arranged by size:
4644 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2352 2702 2864 3060 4424 4783 4942
Size: 648 1186 350 162 196 1364 359 159
Cuts at: 4942 518
Size: 2042
Fragments arranged by size:
2042 1364 1186 648 359 350 196 162
159
MspA1I CmG'CkG
Cuts at: 1606 2474 2873 3882 4559 5707 5952 1606
Size: 868 399 1009 677 1148 245 2120
Fragments arranged by size:
2120 1148 1009 868 677 399 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4975
Fragments arranged by size:
4975 939 552
NdeI CA'TA_TG
Cuts at: 387 3709 387
Size: 3322 3144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NsiI A_TGCA'T
Cuts at: 2197 3641 2197
Size: 1444 5022
NspI r_CATG'y
Cuts at: 1132 1229 2111 3824 5369 1132
Size: 97 882 1713 1545 2229
Fragments arranged by size:
2229 1713 1545 882 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6466
PciI A'CATG_T
Cuts at: 5365 5365
Size: 6466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2331 1108
Size: 1223 5243
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2325 1613
Size: 712 5754
PsiI TTA'TAA
Cuts at: 2088 4036 2088
Size: 1948 4518
PstI C_TGCA'G
Cuts at: 830 2275 3254 3818 830
Size: 1445 979 564 3478
Fragments arranged by size:
3478 1445 979 564
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
PvuII CAG'CTG
Cuts at: 2474 2873 2474
Size: 399 6067
SacI G_AGCT'C
Cuts at: 3145 3145
Size: 6466
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SbfI CC_TGCA'GG
Cuts at: 3818 3818
Size: 6466
ScaI AGT'ACT
Cuts at: 4714 6426 4714
Size: 1712 4754
SfcI C'TryA_G
Cuts at: 826 1045 2271 3250 3414 3814 4949 5630 5821
Size: 219 1226 979 164 400 1135 681 191
Cuts at: 5821 826
Size: 1471
Fragments arranged by size:
1471 1226 1135 979 681 400 219 191
164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 3793 1872
Size: 1921 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4570 5471 5733 820
Size: 197 926 2627 901 262 1553
Fragments arranged by size:
2627 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 1517
Size: 1224 5242
StyI C'CwwG_G
Cuts at: 513 1065 2004 3905 513
Size: 552 939 1901 3074
Fragments arranged by size:
3074 1901 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 4493 4510 4663 4848 5293 325
Size: 1255 486 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 486 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4712 6424 96
Size: 274 80 33 51 1443 2735 1712 138
Fragments arranged by size:
2735 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3006 3038 6426 6458 3006
Size: 32 3388 32 3014
Fragments arranged by size:
3388 3014 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3377 1316
Size: 2061 4405
XbaI T'CTAG_A
Cuts at: 3802 3802
Size: 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2225 2225
Size: 6466
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6466
XmaI C'CCGG_G
Cuts at: 1870 3791 1870
Size: 1921 4545
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 6466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4274 276
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI
BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI