Restriction map for
pFN21AE4151
(Circular) (Six-base) MAPSORT of: pFN21AE4151.seq Check: 3855 from: 1 to: 6463
pFN21AE4151 6463 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3018 3806 1937
Size: 1081 788 4594
Fragments arranged by size:
4594 1081 788
Acc65I G'GTAC_C
Cuts at: 2808 3784 2808
Size: 976 5487
AclI AA'CG_TT
Cuts at: 1563 2129 4590 4963 1563
Size: 566 2461 373 3063
Fragments arranged by size:
3063 2461 566 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2547 4526 5910 6178 1632
Size: 915 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 915 268
AfeI AGC'GCT
Cuts at: 3263 3263
Size: 6463
AflII C'TTAA_G
Cuts at: 820 1017 2731 2783 820
Size: 197 1714 52 4500
Fragments arranged by size:
4500 1714 197 52
AflIII A'CryG_T
Cuts at: 1203 5362 6339 1203
Size: 4159 977 1327
Fragments arranged by size:
4159 1327 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6463
AlwNI CAG_nnn'CTG
Cuts at: 2015 4206 5778 2015
Size: 2191 1572 2700
Fragments arranged by size:
2700 2191 1572
ApaLI G'TGCA_C
Cuts at: 4520 5676 4520
Size: 1156 5307
ApoI r'AATT_y
Cuts at: 1493 2303 2322 2546 2725 3153 3772 3998 4150
Size: 810 19 224 179 428 619 226 152
Cuts at: 4150 6259 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 810 619 428 226 224 179
152 19
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 3117 3788 1870
Size: 73 121 1053 671 4545
Fragments arranged by size:
4545 1053 671 121 73
BamHI G'GATC_C
Cuts at: 3793 4160 3793
Size: 367 6096
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2808 3784 5239 618
Size: 325 217 627 1021 976 1455 1842
Fragments arranged by size:
1842 1455 1021 976 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3782 1391
Size: 2391 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 928
Size: 971 149 5343
Fragments arranged by size:
5343 971 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3045 3079 4654 4688 1903
Size: 34 1108 34 1575 34 3678
Fragments arranged by size:
3678 1575 1108 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4355 5565 4355
Size: 1210 5253
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5074 136
Size: 107 122 71 631 605 3402 1525
Fragments arranged by size:
3402 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6458 6458
Size: 6463
BlpI GC'TnA_GC
Cuts at: 3880 3880
Size: 6463
Bme1580I G_kGCm'C
Cuts at: 1790 4524 5680 1790
Size: 2734 1156 2573
Fragments arranged by size:
2734 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2140 1318
Size: 822 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3110 4194 5152
Size: 416 53 83 399 24 1693 1084 958
Cuts at: 5152 442
Size: 1753
Fragments arranged by size:
1753 1693 1084 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2462 1056
Size: 1406 5057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2808 3186 5123 1239
Size: 1569 378 1937 2579
Fragments arranged by size:
2579 1937 1569 378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4588 5453 5751 4588
Size: 865 298 5300
Fragments arranged by size:
5300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3541 3743 5126 882
Size: 617 2042 202 1383 2219
Fragments arranged by size:
2219 2042 1383 617 202
BsaAI yAC'GTr
Cuts at: 493 1204 4254 493
Size: 711 3050 2702
Fragments arranged by size:
3050 2702 711
BsaBI GATnn'nnATC
Cuts at: 6457 6457
Size: 6463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 4270 4652 5287
Size: 53 83 186 936 567 2170 382 635
Cuts at: 5287 275
Size: 1451
Fragments arranged by size:
2170 1451 936 635 567 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4896 5294 5568 5715 6126 4896
Size: 398 274 147 411 5233
Fragments arranged by size:
5233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6238
Fragments arranged by size:
6238 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6463
BseYI C'CCAG_C
Cuts at: 3245 3258 5666 6162 3245
Size: 13 2408 496 3546
Fragments arranged by size:
3546 2408 496 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3594 1163
Size: 573 1858 4032
Fragments arranged by size:
4032 1858 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4524 4609 5680 4524
Size: 85 1071 5307
Fragments arranged by size:
5307 1071 85
BsmI GAATG_Cn'
Cuts at: 2783 3974 4067 2783
Size: 1191 93 5179
Fragments arranged by size:
5179 1191 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3782 4524 4609 5680 1391
Size: 399 1992 742 85 1071 2174
Fragments arranged by size:
2174 1992 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6126 6126
Size: 6463
BspHI T'CATG_A
Cuts at: 2103 4352 6082 2103
Size: 2249 1730 2484
Fragments arranged by size:
2484 2249 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2179 3818 844
Size: 1335 1639 3489
Fragments arranged by size:
3489 1639 1335
BsrBI CCG'CTC
Cuts at: 1394 1823 4350 1394
Size: 429 2527 3507
Fragments arranged by size:
3507 2527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4958 5132 65
Size: 1183 3710 174 1396
Fragments arranged by size:
3710 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5107 5294 1700
Size: 110 3297 187 2869
Fragments arranged by size:
3297 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 4523 5535 4523
Size: 1012 5451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3140 794
Size: 2346 4117
BstBI TT'CG_AA
Cuts at: 3095 5267 3095
Size: 2172 4291
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstXI CCAn_nnnn'nTGG
Cuts at: 3549 3549
Size: 6463
BstYI r'GATC_y
Cuts at: 1972 3793 4160 4545 4562 6014 6100 6112 6458
Size: 1821 367 385 17 1452 86 12 346
Cuts at: 6458 1972
Size: 1977
Fragments arranged by size:
1977 1821 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3019 3019
Size: 6463
BtgI C'CryG_G
Cuts at: 513 1065 2004 3271 3548 513
Size: 552 939 1267 277 3428
Fragments arranged by size:
3428 1267 939 552 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 3979 4773 4793 3979
Size: 794 20 5649
Fragments arranged by size:
5649 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 3767 3767
Size: 6463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5470 809
Size: 507 4154 1802
Fragments arranged by size:
4154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3910 4799 6428
Size: 54 1425 50 275 18 2080 889 1629
Cuts at: 6428 8
Size: 43
Fragments arranged by size:
2080 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2977 3741 4393 1353
Size: 668 956 764 652 3423
Fragments arranged by size:
3423 956 764 668 652
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5050 5424 5570 387
Size: 1064 138 3461 374 146 1280
Fragments arranged by size:
3461 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2547 2808 3186 4526 5123 5910 6178
Size: 393 915 261 378 1340 597 787 268
Cuts at: 6178 1239
Size: 1524
Fragments arranged by size:
1524 1340 915 787 597 393 378 268
261
EcoO109I rG'GnC_Cy
Cuts at: 3104 3104
Size: 6463
EcoRI G'AATT_C
Cuts at: 3772 4150 3772
Size: 378 6085
EcoRV GAT'ATC
Cuts at: 2689 2689
Size: 6463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 3770 2746
Size: 1024 5439
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 3265 5610 1678
Size: 1587 2345 2531
Fragments arranged by size:
2531 2345 1587
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 2797 2829 3624
Size: 32 135 32 586 32 607 32 795
Cuts at: 3624 3656 5104 5136 5178 5210 1373
Size: 32 1448 32 42 32 2626
Fragments arranged by size:
2626 1448 795 607 586 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3807 4053 669
Size: 988 281 1869 246 3079
Fragments arranged by size:
3079 1869 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6463
HpaI GTT'AAC
Cuts at: 4053 4053
Size: 6463
KpnI G_GTAC'C
Cuts at: 2812 3788 2812
Size: 976 5487
MfeI C'AATT_G
Cuts at: 4062 4062
Size: 6463
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 3134 5577 5761 1305
Size: 1032 797 2443 184 2007
Fragments arranged by size:
2443 2007 1032 797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4641
Fragments arranged by size:
4641 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4421 4780 4939 518
Size: 648 3255 359 159 2042
Fragments arranged by size:
3255 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2964 3879 4556 5704 5949 1606
Size: 1358 915 677 1148 245 2120
Fragments arranged by size:
2120 1358 1148 915 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3271 3548 513
Size: 552 939 1267 277 3428
Fragments arranged by size:
3428 1267 939 552 277
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6463
NheI G'CTAG_C
Cuts at: 1052 2458 1052
Size: 1406 5057
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 2116 2236 2997 3138 3253 3821 5366
Size: 97 887 120 761 141 115 568 1545
Cuts at: 5366 1132
Size: 2229
Fragments arranged by size:
2229 1545 887 761 568 141 120 115
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6463
PciI A'CATG_T
Cuts at: 5362 5362
Size: 6463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3277 1108
Size: 2169 4294
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 6463
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 6045 6077 4532
Size: 32 1481 32 4918
Fragments arranged by size:
4918 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3104 3104
Size: 6463
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 4033 4033
Size: 6463
PstI C_TGCA'G
Cuts at: 830 2197 2500 2638 3145 3269 3815 830
Size: 1367 303 138 507 124 546 3478
Fragments arranged by size:
3478 1367 546 507 303 138 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SapI GCTCTTCn'nnn_
Cuts at: 2021 2021
Size: 6463
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 4711 6423 4711
Size: 1712 4751
SfcI C'TryA_G
Cuts at: 826 1045 2193 2496 2634 3141 3265 3588 3811
Size: 219 1148 303 138 507 124 323 223
Cuts at: 3811 4946 5627 5818 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1148 1135 681 507 323 303 223
219 191 138 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3790 1872
Size: 1918 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2731 2783 3117 4567 5468 5730
Size: 197 926 788 52 334 1450 901 262
Cuts at: 5730 820
Size: 1553
Fragments arranged by size:
1553 1450 926 901 788 334 262 197
52
SnaBI TAC'GTA
Cuts at: 493 4254 493
Size: 3761 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 2997 3253 3821 1132
Size: 1865 256 568 3774
Fragments arranged by size:
3774 1865 568 256
SspI AAT'ATT
Cuts at: 5 52 3503 4387 5
Size: 47 3451 884 2081
Fragments arranged by size:
3451 2081 884 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6463
StyI C'CwwG_G
Cuts at: 513 1065 2004 3271 3548 3902 513
Size: 552 939 1267 277 354 3074
Fragments arranged by size:
3074 1267 939 552 354 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 2847 3452 4490 4507 4660 4845
Size: 1255 480 787 605 1038 17 153 185
Cuts at: 4845 5290 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1038 787 605 480 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4709 6421 96
Size: 274 80 33 51 1443 2732 1712 138
Fragments arranged by size:
2732 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6423 6455 6423
Size: 32 6431
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6463
XbaI T'CTAG_A
Cuts at: 2470 3799 2470
Size: 1329 5134
XhoI C'TCGA_G
Cuts at: 1943 3117 1943
Size: 1174 5289
XmaI C'CCGG_G
Cuts at: 1870 3788 1870
Size: 1918 4545
XmnI GAAnn'nnTTC
Cuts at: 2550 4592 2550
Size: 2042 4421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI