Restriction map for
pFN21AE4150
(Circular) (Six-base) MAPSORT of: pFN21AE4150.seq Check: 7215 from: 1 to: 6460
pFN21AE4150 6460 bp
With 182 enzymes: SgfI *
March 28, 2012 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4270 278
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3574 3803 1937
Size: 1637 229 4594
Fragments arranged by size:
4594 1637 229
Acc65I G'GTAC_C
Cuts at: 3781 3781
Size: 6460
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 4523 5907 6175 1632
Size: 945 1946 1384 268 1917
Fragments arranged by size:
1946 1917 1384 945 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6263
AflIII A'CryG_T
Cuts at: 1203 5359 6336 1203
Size: 4156 977 1327
Fragments arranged by size:
4156 1327 977
AgeI A'CCGG_T
Cuts at: 5291 5291
Size: 6460
AhdI GACnn_n'nnGTC
Cuts at: 5189 5189
Size: 6460
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6460
AlwNI CAG_nnn'CTG
Cuts at: 2336 4203 5775 2336
Size: 1867 1572 3021
Fragments arranged by size:
3021 1867 1572
ApaLI G'TGCA_C
Cuts at: 4517 5673 4517
Size: 1156 5304
ApoI r'AATT_y
Cuts at: 1493 2181 2463 2533 3014 3106 3769 3995 4147
Size: 688 282 70 481 92 663 226 152
Cuts at: 4147 6256 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 688 663 481 282 226 152
92 70
AseI AT'TA_AT
Cuts at: 160 1032 5014 160
Size: 872 3982 1606
Fragments arranged by size:
3982 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 3785 1870
Size: 73 1842 4545
Fragments arranged by size:
4545 1842 73
BamHI G'GATC_C
Cuts at: 3790 4157 3790
Size: 367 6093
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2989 3295 3781 5236 618
Size: 325 217 627 1202 306 486 1455 1842
Fragments arranged by size:
1842 1455 1202 627 486 325 306 217
BanII G_rGCy'C
Cuts at: 1391 3779 1391
Size: 2388 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3648 928
Size: 971 1749 3740
Fragments arranged by size:
3740 1749 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2053 2087 4651 4685 1903
Size: 34 50 34 32 34 2564 34 3678
Fragments arranged by size:
3678 2564 50 34 34 34 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 4352 5562 4352
Size: 1210 5250
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BfrBI ATG'CAT
Cuts at: 2286 2648 2286
Size: 362 6098
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5071 136
Size: 107 122 71 631 605 3399 1525
Fragments arranged by size:
3399 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6455 6455
Size: 6460
BlpI GC'TnA_GC
Cuts at: 3877 3877
Size: 6460
Bme1580I G_kGCm'C
Cuts at: 1790 4521 5677 1790
Size: 2731 1156 2573
Fragments arranged by size:
2731 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6460
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4191 5149 442
Size: 416 53 83 399 24 2774 958 1753
Fragments arranged by size:
2774 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2375 5120 1239
Size: 1049 87 2745 2579
Fragments arranged by size:
2745 2579 1049 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4585 5450 5748 4585
Size: 865 298 5297
Fragments arranged by size:
5297 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5123 882
Size: 617 3624 2219
Fragments arranged by size:
3624 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3702 4251 493
Size: 711 2498 549 2702
Fragments arranged by size:
2702 2498 711 549
BsaBI GATnn'nnATC
Cuts at: 3549 6454 3549
Size: 2905 3555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3084 4267 4649 5284
Size: 53 83 186 936 1551 1183 382 635
Cuts at: 5284 275
Size: 1451
Fragments arranged by size:
1551 1451 1183 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4893 5291 5565 5712 6123 4893
Size: 398 274 147 411 5230
Fragments arranged by size:
5230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3177 3207 1178
Size: 30 165 30 1774 30 4431
Fragments arranged by size:
4431 1774 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6460
BseYI C'CCAG_C
Cuts at: 5663 6159 5663
Size: 496 5964
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2925 3695 3738 1163
Size: 573 1189 770 43 3885
Fragments arranged by size:
3885 1189 770 573 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3910 4671 5699 1540
Size: 275 186 1909 761 1028 2301
Fragments arranged by size:
2301 1909 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2890 4521 4606 5677 2890
Size: 1631 85 1071 3673
Fragments arranged by size:
3673 1631 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 3122 3971 4064 2095
Size: 1027 849 93 4491
Fragments arranged by size:
4491 1027 849 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2890 3779 4521 4606 5677 1391
Size: 399 1100 889 742 85 1071 2174
Fragments arranged by size:
2174 1100 1071 889 742 399 85
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 4349 6079 4349
Size: 1730 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3815 844
Size: 2971 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4347 1394
Size: 429 2524 3507
Fragments arranged by size:
3507 2524 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4955 5129 65
Size: 1183 3707 174 1396
Fragments arranged by size:
3707 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5104 5291 1700
Size: 110 3294 187 2869
Fragments arranged by size:
3294 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2674 96
Size: 2578 3882
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6460
BssSI C'ACGA_G
Cuts at: 4520 5532 4520
Size: 1012 5448
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6460
BstBI TT'CG_AA
Cuts at: 5264 5264
Size: 6460
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6460
BstYI r'GATC_y
Cuts at: 1972 3790 4157 4542 4559 6011 6097 6109 6455
Size: 1818 367 385 17 1452 86 12 346
Cuts at: 6455 1972
Size: 1977
Fragments arranged by size:
1977 1818 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3217 3217
Size: 6460
BtgI C'CryG_G
Cuts at: 513 1065 3438 513
Size: 552 2373 3535
Fragments arranged by size:
3535 2373 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5721
Fragments arranged by size:
5721 683 56
BtsI GCAGTG_nn'
Cuts at: 3976 4770 4790 3976
Size: 794 20 5646
Fragments arranged by size:
5646 794 20
ClaI AT'CG_AT
Cuts at: 1554 3618 1554
Size: 2064 4396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3542 3577 529
Size: 35 2978 35 3412
Fragments arranged by size:
3412 2978 35 35
DraI TTT'AAA
Cuts at: 3764 3764
Size: 6460
DraIII CAC_nnn'GTG
Cuts at: 2197 2197
Size: 6460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5467 809
Size: 507 4151 1802
Fragments arranged by size:
4151 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3907 4796 6425
Size: 54 1425 50 275 18 2077 889 1629
Cuts at: 6425 8
Size: 43
Fragments arranged by size:
2077 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3907 1537
Size: 275 2095 4090
Fragments arranged by size:
4090 2095 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2553 2625 4390 1353
Size: 1200 72 1765 3423
Fragments arranged by size:
3423 1765 1200 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5047 5421 5567 387
Size: 1064 138 3458 374 146 1280
Fragments arranged by size:
3458 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2375 2577 4523 5120 5907 6175
Size: 393 656 87 202 1946 597 787 268
Cuts at: 6175 1239
Size: 1524
Fragments arranged by size:
1946 1524 787 656 597 393 268 202
87
EcoNI CCTnn'n_nnAGG
Cuts at: 3594 3594
Size: 6460
EcoRI G'AATT_C
Cuts at: 3769 4147 3769
Size: 378 6082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2219 2845 2219
Size: 626 5834
FspI TGC'GCA
Cuts at: 4966 4966
Size: 6460
HaeII r_GCGC'y
Cuts at: 1678 5607 1678
Size: 3929 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2863 2895 3221
Size: 32 135 32 470 32 789 32 326
Cuts at: 3221 3253 3677 3709 3715 3747 5101 5133 5175
Size: 32 424 32 6 32 1354 32 42
Cuts at: 5175 5207 1373
Size: 32 2626
Fragments arranged by size:
2626 1354 789 470 424 326 135 42
32 32 32 32 32 32 32 32
32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3804 4050 669
Size: 988 281 1812 54 246 3079
Fragments arranged by size:
3079 1812 988 281 246 54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6460
HpaI GTT'AAC
Cuts at: 3750 4050 3750
Size: 300 6160
KpnI G_GTAC'C
Cuts at: 3785 3785
Size: 6460
MfeI C'AATT_G
Cuts at: 4059 4059
Size: 6460
MluI A'CGCG_T
Cuts at: 6336 6336
Size: 6460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5574 5758 1305
Size: 4269 184 2007
Fragments arranged by size:
4269 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4638
Fragments arranged by size:
4638 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4418 4777 4936 518
Size: 648 3252 359 159 2042
Fragments arranged by size:
3252 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3332 3716 3876 4553 5701 5946 1606
Size: 1726 384 160 677 1148 245 2120
Fragments arranged by size:
2120 1726 1148 677 384 245 160
NcoI C'CATG_G
Cuts at: 513 1065 3438 513
Size: 552 2373 3535
Fragments arranged by size:
3535 2373 552
NdeI CA'TA_TG
Cuts at: 387 2485 2525 387
Size: 2098 40 4322
Fragments arranged by size:
4322 2098 40
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6460
NotI GC'GGCC_GC
Cuts at: 3907 3907
Size: 6460
NsiI A_TGCA'T
Cuts at: 2288 2650 2288
Size: 362 6098
NspI r_CATG'y
Cuts at: 1132 1229 2074 2582 3818 5363 1132
Size: 97 845 508 1236 1545 2229
Fragments arranged by size:
2229 1545 1236 845 508 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6460
PciI A'CATG_T
Cuts at: 5359 5359
Size: 6460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6460
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2832 2864 4529 4561 6042 6074 2832
Size: 32 1665 32 1481 32 3218
Fragments arranged by size:
3218 1665 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6460
PsiI TTA'TAA
Cuts at: 4030 4030
Size: 6460
PstI C_TGCA'G
Cuts at: 830 2115 3331 3812 830
Size: 1285 1216 481 3478
Fragments arranged by size:
3478 1285 1216 481
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
PvuII CAG'CTG
Cuts at: 3716 3716
Size: 6460
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 2898 3178 3195 4708 6420 2898
Size: 280 17 1513 1712 2938
Fragments arranged by size:
2938 1712 1513 280 17
SfcI C'TryA_G
Cuts at: 826 1045 2111 3252 3327 3808 4943 5624 5815
Size: 219 1066 1141 75 481 1135 681 191
Cuts at: 5815 826
Size: 1471
Fragments arranged by size:
1471 1141 1135 1066 681 481 219 191
75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3787 1872
Size: 1915 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4564 5465 5727 820
Size: 197 926 2621 901 262 1553
Fragments arranged by size:
2621 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4251 493
Size: 3758 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3818 1132
Size: 2686 3774
SspI AAT'ATT
Cuts at: 5 52 4384 5
Size: 47 4332 2081
Fragments arranged by size:
4332 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6460
StyI C'CwwG_G
Cuts at: 513 1065 3438 3899 513
Size: 552 2373 461 3074
Fragments arranged by size:
3074 2373 552 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3458 3488 4487 4504 4657 4842 5287
Size: 1255 1878 30 999 17 153 185 445
Cuts at: 5287 325
Size: 1498
Fragments arranged by size:
1878 1498 1255 999 445 185 153 30
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2674 2896 2955
Size: 274 80 33 51 1443 697 222 59
Cuts at: 2955 3176 3193 3261 4706 6418 96
Size: 221 17 68 1445 1712 138
Fragments arranged by size:
1712 1445 1443 697 274 222 221 138
80 68 59 51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6420 6452 6420
Size: 32 6428
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6460
XbaI T'CTAG_A
Cuts at: 3151 3796 3151
Size: 645 5815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2444 2444
Size: 6460
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3785 1870
Size: 1915 4545
XmnI GAAnn'nnTTC
Cuts at: 2106 2248 4589 2106
Size: 142 2341 3977
Fragments arranged by size:
3977 2341 142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4268 276
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI