Restriction map for
pFN21AE4143
(Circular) (Six-base) MAPSORT of: pFN21AE4143.seq Check: 5426 from: 1 to: 6412
pFN21AE4143 6412 bp
With 182 enzymes: SgfI *
March 28, 2012 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2989 3562 3601 2989
Size: 573 39 5800
Fragments arranged by size:
5800 573 39
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2554 3693 4222 278
Size: 53 83 186 936 1018 1139 529 2468
Fragments arranged by size:
2468 1139 1018 936 529 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 3755 1937
Size: 870 948 4594
Fragments arranged by size:
4594 948 870
Acc65I G'GTAC_C
Cuts at: 3733 3733
Size: 6412
AclI AA'CG_TT
Cuts at: 1563 4539 4912 1563
Size: 2976 373 3063
Fragments arranged by size:
3063 2976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 2399 2522 4475 5859 6127 1632
Size: 704 63 123 1953 1384 268 1917
Fragments arranged by size:
1953 1917 1384 704 268 123 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6215
AflIII A'CryG_T
Cuts at: 1203 5311 6288 1203
Size: 4108 977 1327
Fragments arranged by size:
4108 1327 977
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 2080 5141 2080
Size: 3061 3351
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2855 1184
Size: 1671 4741
AlwNI CAG_nnn'CTG
Cuts at: 2389 2666 2770 3037 3262 3265 3607 4155 5727
Size: 277 104 267 225 3 342 548 1572
Cuts at: 5727 2389
Size: 3074
Fragments arranged by size:
3074 1572 548 342 277 267 225 104
3
ApaI G_GGCC'C
Cuts at: 3295 3295
Size: 6412
ApaLI G'TGCA_C
Cuts at: 4469 5625 4469
Size: 1156 5256
ApoI r'AATT_y
Cuts at: 1493 3721 3947 4099 6208 1493
Size: 2228 226 152 2109 1697
Fragments arranged by size:
2228 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4966 160
Size: 872 3934 1606
Fragments arranged by size:
3934 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 2394 2604 2825 3737 1870
Size: 73 451 210 221 912 4545
Fragments arranged by size:
4545 912 451 221 210 73
BamHI G'GATC_C
Cuts at: 2608 3627 3742 4109 2608
Size: 1019 115 367 4911
Fragments arranged by size:
4911 1019 367 115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2813 3676 3733 5188
Size: 325 217 627 363 663 863 57 1455
Cuts at: 5188 618
Size: 1842
Fragments arranged by size:
1842 1455 863 663 627 363 325 217
57
BanII G_rGCy'C
Cuts at: 1391 2124 2777 2952 3165 3203 3295 3731 1391
Size: 733 653 175 213 38 92 436 4072
Fragments arranged by size:
4072 733 653 436 213 175 92 38
BbeI G_GCGC'C
Cuts at: 2154 3680 2154
Size: 1526 4886
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5441
BbvCI CC'TCA_GC
Cuts at: 2516 2667 3187 3404 2516
Size: 151 520 217 5524
Fragments arranged by size:
5524 520 217 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4603 4637 1903
Size: 34 2666 34 3678
Fragments arranged by size:
3678 2666 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4304 5514 4304
Size: 1210 5202
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2669 2766
Size: 107 122 71 631 605 337 660 97
Cuts at: 2766 5023 136
Size: 2257 1525
Fragments arranged by size:
2257 1525 660 631 605 337 122 107
97 71
BglII A'GATC_T
Cuts at: 6407 6407
Size: 6412
BlpI GC'TnA_GC
Cuts at: 2123 2139 2642 3829 2123
Size: 16 503 1187 4706
Fragments arranged by size:
4706 1187 503 16
Bme1580I G_kGCm'C
Cuts at: 1790 2816 3295 4473 5629 1790
Size: 1026 479 1178 1156 2573
Fragments arranged by size:
2573 1178 1156 1026 479
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6412
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4143 5101 442
Size: 416 53 83 399 24 2726 958 1753
Fragments arranged by size:
2726 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2812 2272
Size: 540 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2438 2645 2654 2942 3383 3413 3455
Size: 851 348 207 9 288 441 30 42
Cuts at: 3455 3578 3668 5072 1239
Size: 123 90 1404 2579
Fragments arranged by size:
2579 1404 851 441 348 288 207 123
90 42 30 9
Bpu10I CC'TnA_GC
Cuts at: 2516 2667 2771 3187 3404 2516
Size: 151 104 416 217 5524
Fragments arranged by size:
5524 416 217 151 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4537 5402 5700 4537
Size: 865 298 5249
Fragments arranged by size:
5249 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3554 3635 5075 882
Size: 617 2055 81 1440 2219
Fragments arranged by size:
2219 2055 1440 617 81
BsaAI yAC'GTr
Cuts at: 493 1204 4203 493
Size: 711 2999 2702
Fragments arranged by size:
2999 2702 711
BsaBI GATnn'nnATC
Cuts at: 3338 6406 3338
Size: 3068 3344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 2551 3677 3690
Size: 53 83 186 936 618 400 1126 13
Cuts at: 3690 4219 4601 5236 275
Size: 529 382 635 1451
Fragments arranged by size:
1451 1126 936 635 618 529 400 382
186 83 53 13
BsaWI w'CCGG_w
Cuts at: 4845 5243 5517 5664 6075 4845
Size: 398 274 147 411 5182
Fragments arranged by size:
5182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2684 2714 1178
Size: 30 165 30 1281 30 4876
Fragments arranged by size:
4876 1281 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2366 2510 2540 2651 2698 2734 3236
Size: 1075 105 144 30 111 47 36 502
Cuts at: 3236 3380 3383 3434 3503 1186
Size: 144 3 51 69 4095
Fragments arranged by size:
4095 1075 502 144 144 111 105 69
51 47 36 30 3
BseYI C'CCAG_C
Cuts at: 2368 3236 3498 5615 6111 2368
Size: 868 262 2117 496 2669
Fragments arranged by size:
2669 2117 868 496 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2049 2249 2558 3101 3107 3256 3431
Size: 573 313 200 309 543 6 149 175
Cuts at: 3431 3469 3497 3596 1163
Size: 38 28 99 3979
Fragments arranged by size:
3979 573 543 313 309 200 175 149
99 38 28 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2057 3862 4623 5651 1540
Size: 275 186 56 1805 761 1028 2301
Fragments arranged by size:
2301 1805 1028 761 275 186 56
BsiHKAI G_wGCw'C
Cuts at: 2124 2204 2952 3006 3315 4473 4558 5629 2124
Size: 80 748 54 309 1158 85 1071 2907
Fragments arranged by size:
2907 1158 1071 748 309 85 80 54
BsmI GAATG_Cn'
Cuts at: 3923 4016 3923
Size: 93 6319
BsmBI CGTCTCn'nnnn_
Cuts at: 2542 2783 2959 2542
Size: 241 176 5995
Fragments arranged by size:
5995 241 176
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2204 2777 2816 2952 3006 3165
Size: 399 334 80 573 39 136 54 159
Cuts at: 3165 3203 3295 3315 3731 4473 4558 5629 1391
Size: 38 92 20 416 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 573 416 399 334 159
136 92 85 80 54 39 38 20
BspEI T'CCGG_A
Cuts at: 6075 6075
Size: 6412
BspHI T'CATG_A
Cuts at: 4301 6031 4301
Size: 1730 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2847 2989 3562 3601 3767 844
Size: 2003 142 573 39 166 3489
Fragments arranged by size:
3489 2003 573 166 142 39
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 2362 2646 2941 3076 3472 3625
Size: 429 221 318 284 295 135 396 153
Cuts at: 3625 4299 1394
Size: 674 3507
Fragments arranged by size:
3507 674 429 396 318 295 284 221
153 135
BsrDI GCAATG_nn'
Cuts at: 65 1248 2980 4907 5081 65
Size: 1183 1732 1927 174 1396
Fragments arranged by size:
1927 1732 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2127 5056 5243 1700
Size: 110 210 107 2929 187 2869
Fragments arranged by size:
2929 2869 210 187 110 107
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6412
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 2441 4472 5484 2441
Size: 2031 1012 3369
Fragments arranged by size:
3369 2031 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3037 3262 794
Size: 2243 225 3944
Fragments arranged by size:
3944 2243 225
BstBI TT'CG_AA
Cuts at: 5216 5216
Size: 6412
BstEII G'GTnAC_C
Cuts at: 1184 3103 1184
Size: 1919 4493
BstYI r'GATC_y
Cuts at: 1972 2541 2608 3627 3742 4109 4494 4511 5963
Size: 569 67 1019 115 367 385 17 1452
Cuts at: 5963 6049 6061 6407 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1019 569 385 367 346 115
86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 3455 3455
Size: 6412
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2478 2583 513
Size: 552 939 24 450 105 4342
Fragments arranged by size:
4342 939 552 450 105 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2598 2703 487
Size: 683 56 1372 105 4196
Fragments arranged by size:
4196 1372 683 105 56
BtsI GCAGTG_nn'
Cuts at: 2243 3230 3928 4722 4742 2243
Size: 987 698 794 20 3913
Fragments arranged by size:
3913 987 794 698 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6377
DraI TTT'AAA
Cuts at: 3716 3716
Size: 6412
DraIII CAC_nnn'GTG
Cuts at: 2877 2877
Size: 6412
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5419 809
Size: 507 4103 1802
Fragments arranged by size:
4103 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2054 3378
Size: 54 1425 50 275 18 195 29 1324
Cuts at: 3378 3859 4748 6377 8
Size: 481 889 1629 43
Fragments arranged by size:
1629 1425 1324 889 481 275 195 54
50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2054 3859 1537
Size: 275 242 1805 4090
Fragments arranged by size:
4090 1805 275 242
EarI CTCTTCn'nnn_
Cuts at: 1353 2781 4342 1353
Size: 1428 1561 3423
Fragments arranged by size:
3423 1561 1428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3110 3620 4999 5373 5519 387
Size: 1064 138 1521 510 1379 374 146 1280
Fragments arranged by size:
1521 1379 1280 1064 510 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2950 2122
Size: 828 5584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2336 2399 2438 2522 2645 2654
Size: 393 458 246 63 39 84 123 9
Cuts at: 2654 2942 3383 3413 3455 3578 3668 4475 5072
Size: 288 441 30 42 123 90 807 597
Cuts at: 5072 5859 6127 1239
Size: 787 268 1524
Fragments arranged by size:
1524 807 787 597 458 441 393 288
268 246 123 123 90 84 63 42
39 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 3011 2318
Size: 693 5719
EcoO109I rG'GnC_Cy
Cuts at: 2767 2917 2959 3291 3604 2767
Size: 150 42 332 313 5575
Fragments arranged by size:
5575 332 313 150 42
EcoRI G'AATT_C
Cuts at: 3721 4099 3721
Size: 378 6034
FspI TGC'GCA
Cuts at: 3659 4918 3659
Size: 1259 5153
HaeII r_GCGC'y
Cuts at: 1678 2154 2700 3328 3680 5559 1678
Size: 476 546 628 352 1879 2531
Fragments arranged by size:
2531 1879 628 546 476 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2096 2098 2128 2684
Size: 32 135 32 494 30 2 30 556
Cuts at: 2684 2716 2917 2949 3212 3244 3500 3532 5053
Size: 32 201 32 263 32 256 32 1521
Cuts at: 5053 5085 5127 5159 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1521 556 494 263 256 201 135
42 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3756 4002 669
Size: 988 281 1818 246 3079
Fragments arranged by size:
3079 1818 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6412
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KasI G'GCGC_C
Cuts at: 2150 3676 2150
Size: 1526 4886
KpnI G_GTAC'C
Cuts at: 3737 3737
Size: 6412
MfeI C'AATT_G
Cuts at: 4011 4011
Size: 6412
MluI A'CGCG_T
Cuts at: 6288 6288
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5526 5710 1305
Size: 4221 184 2007
Fragments arranged by size:
4221 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3380 10
Size: 54 1425 343 1548 3042
Fragments arranged by size:
3042 1548 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4370 4729 4888 518
Size: 648 3204 359 159 2042
Fragments arranged by size:
3204 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2030 2233 2386 2623 2666 2887 2908
Size: 409 15 203 153 237 43 221 21
Cuts at: 2908 3034 3085 3178 3262 3403 3415 3828 4505
Size: 126 51 93 84 141 12 413 677
Cuts at: 4505 5653 5898 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 413 409 245 237 221
203 153 141 126 93 84 51 43
21 15 12
NaeI GCC'GGC
Cuts at: 2129 2129
Size: 6412
NarI GG'CG_CC
Cuts at: 2151 3677 2151
Size: 1526 4886
NcoI C'CATG_G
Cuts at: 513 1065 2004 2478 513
Size: 552 939 474 4447
Fragments arranged by size:
4447 939 552 474
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6412
NgoMIV G'CCGG_C
Cuts at: 2127 2127
Size: 6412
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NruI TCG'CGA
Cuts at: 2937 2937
Size: 6412
NspI r_CATG'y
Cuts at: 1132 1229 2564 3770 5315 1132
Size: 97 1335 1206 1545 2229
Fragments arranged by size:
2229 1545 1335 1206 97
PasI CC'CwG_GG
Cuts at: 1348 3196 1348
Size: 1848 4564
PciI A'CATG_T
Cuts at: 5311 5311
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 5994 6026 4481
Size: 32 1481 32 4867
Fragments arranged by size:
4867 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2917 2917
Size: 6412
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6412
PsiI TTA'TAA
Cuts at: 3982 3982
Size: 6412
PspOMI G'GGCC_C
Cuts at: 3291 3291
Size: 6412
PstI C_TGCA'G
Cuts at: 830 2265 2892 2913 3090 3258 3303 3549 3612
Size: 1435 627 21 177 168 45 246 63
Cuts at: 3612 3764 830
Size: 152 3478
Fragments arranged by size:
3478 1435 627 246 177 168 152 63
45 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
PvuII CAG'CTG
Cuts at: 2015 2386 2887 2908 3034 3085 3178 3262 3403
Size: 371 501 21 126 51 93 84 141
Cuts at: 3403 2015
Size: 5024
Fragments arranged by size:
5024 501 371 141 126 93 84 51
21
SacI G_AGCT'C
Cuts at: 2124 2952 2124
Size: 828 5584
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 6412
SalI G'TCGA_C
Cuts at: 1936 3754 1936
Size: 1818 4594
SbfI CC_TGCA'GG
Cuts at: 3612 3764 3612
Size: 152 6260
ScaI AGT'ACT
Cuts at: 4660 6372 4660
Size: 1712 4700
SfcI C'TryA_G
Cuts at: 826 1045 2261 2888 2909 3086 3254 3299 3545
Size: 219 1216 627 21 177 168 45 246
Cuts at: 3545 3608 3760 4895 5576 5767 826
Size: 63 152 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 681 627 246 219 191
177 168 152 63 45 21
SfoI GGC'GCC
Cuts at: 2152 3678 2152
Size: 1526 4886
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 2020 1700
Size: 320 6092
SmaI CCC'GGG
Cuts at: 1872 2396 2606 3739 1872
Size: 524 210 1133 4545
Fragments arranged by size:
4545 1133 524 210
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4516 5417 5679 820
Size: 197 926 2573 901 262 1553
Fragments arranged by size:
2573 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 2564 3770 1132
Size: 1432 1206 3774
Fragments arranged by size:
3774 1432 1206
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 2672 3440 1517
Size: 1155 768 4489
Fragments arranged by size:
4489 1155 768
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 2719 3157 3531 3851 513
Size: 552 939 474 241 438 374 320 3074
Fragments arranged by size:
3074 939 552 474 438 374 320 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3125 4439 4456 4609 4794 5239 325
Size: 1255 1545 1314 17 153 185 445 1498
Fragments arranged by size:
1545 1498 1314 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4658 6370 96
Size: 274 80 33 51 1443 2681 1712 138
Fragments arranged by size:
2681 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2806 2838 6372 6404 2806
Size: 32 3534 32 2814
Fragments arranged by size:
3534 2814 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6412
XbaI T'CTAG_A
Cuts at: 3748 3748
Size: 6412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2874 3388 2179
Size: 695 514 5203
Fragments arranged by size:
5203 695 514
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6412
XmaI C'CCGG_G
Cuts at: 1870 2394 2604 3737 1870
Size: 524 210 1133 4545
Fragments arranged by size:
4545 1133 524 210
XmnI GAAnn'nnTTC
Cuts at: 4541 4541
Size: 6412
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2552 3691 4220 276
Size: 53 83 186 936 1018 1139 529 2468
Fragments arranged by size:
2468 1139 1018 936 529 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I EcoRV FalI FseI FspAI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SrfI SwaI