Restriction map for
pFN21AE4141
(Circular) (Six-base) MAPSORT of: pFN21AE4141.seq Check: 3994 from: 1 to: 6379
pFN21AE4141 6379 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2118 3560 4442 5826 6094 1632
Size: 476 10 1442 882 1384 268 1917
Fragments arranged by size:
1917 1442 1384 882 476 268 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 2951 5278 6255 1203
Size: 1748 2327 977 1327
Fragments arranged by size:
2327 1748 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2454 1184
Size: 1270 5109
AlwNI CAG_nnn'CTG
Cuts at: 4122 5694 4122
Size: 1572 4807
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 2222 2444 2484 2525 3688 3914 4066 6175
Size: 729 222 40 41 1163 226 152 2109
Cuts at: 6175 1493
Size: 1697
Fragments arranged by size:
2109 1697 1163 729 226 222 152 41
40
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 3704 1870
Size: 73 1761 4545
Fragments arranged by size:
4545 1761 73
BamHI G'GATC_C
Cuts at: 2881 3709 4076 2881
Size: 828 367 5184
Fragments arranged by size:
5184 828 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3509 3700 5155 618
Size: 325 217 627 1722 191 1455 1842
Fragments arranged by size:
1842 1722 1455 627 325 217 191
BanII G_rGCy'C
Cuts at: 1391 3148 3698 1391
Size: 1757 550 4072
Fragments arranged by size:
4072 1757 550
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2637 2671 4570 4604 1903
Size: 34 700 34 1899 34 3678
Fragments arranged by size:
3678 1899 700 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4271 5481 3542
Size: 729 1210 4440
Fragments arranged by size:
4440 1210 729
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BfrBI ATG'CAT
Cuts at: 2078 2288 2794 2078
Size: 210 506 5663
Fragments arranged by size:
5663 506 210
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3279 4990 136
Size: 107 122 71 631 605 1607 1711 1525
Fragments arranged by size:
1711 1607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3601 6374 3601
Size: 2773 3606
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 4440 5596 1790
Size: 2650 1156 2573
Fragments arranged by size:
2650 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2988 3630 4110
Size: 416 53 83 399 24 1571 642 480
Cuts at: 4110 5068 442
Size: 958 1753
Fragments arranged by size:
1753 1571 958 642 480 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3132 3340 3564 5039 1239
Size: 1893 208 224 1475 2579
Fragments arranged by size:
2579 1893 1475 224 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 4504 5369 5667 2357
Size: 2147 865 298 3069
Fragments arranged by size:
3069 2147 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3654 5042 882
Size: 617 2155 1388 2219
Fragments arranged by size:
2219 2155 1388 617
BsaAI yAC'GTr
Cuts at: 493 1204 4170 493
Size: 711 2966 2702
Fragments arranged by size:
2966 2702 711
BsaBI GATnn'nnATC
Cuts at: 2694 3600 6373 2694
Size: 906 2773 2700
Fragments arranged by size:
2773 2700 906
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4186 4568 5203 275
Size: 53 83 186 936 2653 382 635 1451
Fragments arranged by size:
2653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6154
Fragments arranged by size:
6154 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6379
BseYI C'CCAG_C
Cuts at: 5582 6078 5582
Size: 496 5883
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3114 3280 1163
Size: 573 1378 166 4262
Fragments arranged by size:
4262 1378 573 166
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3386 4440 4525 5596 3386
Size: 1054 85 1071 4169
Fragments arranged by size:
4169 1071 1054 85
BsmI GAATG_Cn'
Cuts at: 2078 2433 2660 3446 3890 3983 2078
Size: 355 227 786 444 93 4474
Fragments arranged by size:
4474 786 444 355 227 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3148 3386 3698 4440 4525 5596 1391
Size: 399 1358 238 312 742 85 1071 2174
Fragments arranged by size:
2174 1358 1071 742 399 312 238 85
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 2632 3519 4268 5998 2632
Size: 887 749 1730 3013
Fragments arranged by size:
3013 1730 887 749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 844
Size: 2890 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2386 2763 4266 1394
Size: 429 563 377 1503 3507
Fragments arranged by size:
3507 1503 563 429 377
BsrDI GCAATG_nn'
Cuts at: 65 1248 2202 4874 5048 65
Size: 1183 954 2672 174 1396
Fragments arranged by size:
2672 1396 1183 954 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6379
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6379
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2347 2695 2757 2881 3024 3601 3709 4076
Size: 375 348 62 124 143 577 108 367
Cuts at: 4076 4461 4478 5930 6016 6028 6374 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 577 385 375 367 348 346
143 124 108 86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 2719 2719
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 3895 4689 4709 3895
Size: 794 20 5565
Fragments arranged by size:
5565 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 2227 3180 3683 2227
Size: 953 503 4923
Fragments arranged by size:
4923 953 503
DraIII CAC_nnn'GTG
Cuts at: 3021 3021
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3271 3826 4715
Size: 54 1425 50 275 18 1441 555 889
Cuts at: 4715 6344 8
Size: 1629 43
Fragments arranged by size:
1629 1441 1425 889 555 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3150 4309 1353
Size: 774 1023 1159 3423
Fragments arranged by size:
3423 1159 1023 774
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2118 3132 3340 3560 3564 4442
Size: 393 476 10 1014 208 220 4 878
Cuts at: 4442 5039 5826 6094 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1014 878 787 597 476 393 268
220 208 10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2336
Size: 6379
EcoO109I rG'GnC_Cy
Cuts at: 2960 2960
Size: 6379
EcoRI G'AATT_C
Cuts at: 2484 3688 4066 2484
Size: 1204 378 4797
Fragments arranged by size:
4797 1204 378
EcoRV GAT'ATC
Cuts at: 3331 3331
Size: 6379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 6379
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 5526 1678
Size: 3848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 5020 5052 5094
Size: 32 135 32 887 32 2529 32 42
Cuts at: 5094 5126 1373
Size: 32 2626
Fragments arranged by size:
2626 2529 887 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3723 3969 669
Size: 988 281 1785 246 3079
Fragments arranged by size:
3079 1785 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5493 5677 1305
Size: 4188 184 2007
Fragments arranged by size:
4188 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3273 10
Size: 54 1425 343 1441 3116
Fragments arranged by size:
3116 1441 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3657 4337 4696 4855 518
Size: 648 2491 680 359 159 2042
Fragments arranged by size:
2491 2042 680 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2207 2345 3795 4472 5620 5865 1606
Size: 601 138 1450 677 1148 245 2120
Fragments arranged by size:
2120 1450 1148 677 601 245 138
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
NdeI CA'TA_TG
Cuts at: 387 2876 387
Size: 2489 3890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NsiI A_TGCA'T
Cuts at: 2080 2290 2796 2080
Size: 210 506 5663
Fragments arranged by size:
5663 506 210
NspI r_CATG'y
Cuts at: 1132 1229 2955 3737 5282 1132
Size: 97 1726 782 1545 2229
Fragments arranged by size:
2229 1726 1545 782 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 2951 5278 2951
Size: 2327 4052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3374 1108
Size: 2266 4113
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 4448 4480 5961 5993 3149
Size: 32 1267 32 1481 32 3535
Fragments arranged by size:
3535 1481 1267 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2960 2960
Size: 6379
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6379
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PstI C_TGCA'G
Cuts at: 830 3731 830
Size: 2901 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 2207 2207
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 2354 2986 3283 3727 4862 5543 5734
Size: 219 1309 632 297 444 1135 681 191
Cuts at: 5734 826
Size: 1471
Fragments arranged by size:
1471 1309 1135 681 632 444 297 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 4483 5384 5646 820
Size: 197 926 393 2147 901 262 1553
Fragments arranged by size:
2147 1553 926 901 393 262 197
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 2274 2724 1517
Size: 757 450 5172
Fragments arranged by size:
5172 757 450
StyI C'CwwG_G
Cuts at: 513 1065 2004 3102 3818 513
Size: 552 939 1098 716 3074
Fragments arranged by size:
3074 1098 939 716 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3095 3645 4406 4423 4576 4761 5206
Size: 1255 1515 550 761 17 153 185 445
Cuts at: 5206 325
Size: 1498
Fragments arranged by size:
1515 1498 1255 761 550 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2703 4625 6337
Size: 274 80 33 51 1443 726 1922 1712
Cuts at: 6337 96
Size: 138
Fragments arranged by size:
1922 1712 1443 726 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3343 3375 6339 6371 3343
Size: 32 2964 32 3351
Fragments arranged by size:
3351 2964 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2619 2619
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 2526 2550 4508 2526
Size: 24 1958 4397
Fragments arranged by size:
4397 1958 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI