Restriction map for
pFN21AE4140
(Circular) (Six-base) MAPSORT of: pFN21AE4140.seq Check: 3377 from: 1 to: 6376
pFN21AE4140 6376 bp
With 182 enzymes: SgfI *
March 28, 2012 13:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3607 3719 1937
Size: 1670 112 4594
Fragments arranged by size:
4594 1670 112
Acc65I G'GTAC_C
Cuts at: 3243 3697 3243
Size: 454 5922
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3398 4439 5823 6091 1632
Size: 1766 1041 1384 268 1917
Fragments arranged by size:
1917 1766 1384 1041 268
AfeI AGC'GCT
Cuts at: 2221 2221
Size: 6376
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 3351 5275 6252 1203
Size: 2148 1924 977 1327
Fragments arranged by size:
2148 1924 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 3512 1166
Size: 2346 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3133 1184
Size: 1949 4427
AlwNI CAG_nnn'CTG
Cuts at: 3286 3376 3597 4119 5691 3286
Size: 90 221 522 1572 3971
Fragments arranged by size:
3971 1572 522 221 90
ApaI G_GGCC'C
Cuts at: 2232 2232
Size: 6376
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 3685 3911 4063 6172 1493
Size: 2192 226 152 2109 1697
Fragments arranged by size:
2192 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2496 4930 160
Size: 872 1464 2434 1606
Fragments arranged by size:
2434 1606 1464 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 3520 3701 1870
Size: 73 210 1367 181 4545
Fragments arranged by size:
4545 1367 210 181 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3154 3187 3154
Size: 33 6343
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2904 3243 3697 5152
Size: 325 217 627 285 832 339 454 1455
Cuts at: 5152 618
Size: 1842
Fragments arranged by size:
1842 1455 832 627 454 339 325 285
217
BanII G_rGCy'C
Cuts at: 1391 2154 2232 3695 1391
Size: 763 78 1463 4072
Fragments arranged by size:
4072 1463 763 78
BbeI G_GCGC'C
Cuts at: 2076 2908 2076
Size: 832 5544
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 3427 928
Size: 971 201 1327 3877
Fragments arranged by size:
3877 1327 971 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4567 4601 1903
Size: 34 2630 34 3678
Fragments arranged by size:
3678 2630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2363 4268 5478 2363
Size: 1905 1210 3261
Fragments arranged by size:
3261 1905 1210
BclI T'GATC_A
Cuts at: 1548 2748 1548
Size: 1200 5176
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2565 3493 3652 6371 2565
Size: 928 159 2719 2570
Fragments arranged by size:
2719 2570 928 159
BlpI GC'TnA_GC
Cuts at: 3793 3793
Size: 6376
Bme1580I G_kGCm'C
Cuts at: 1790 2232 4437 5593 1790
Size: 442 2205 1156 2573
Fragments arranged by size:
2573 2205 1156 442
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4107 5065 442
Size: 416 53 83 399 24 2690 958 1753
Fragments arranged by size:
2690 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3535 3535
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 2659 5036 1239
Size: 1247 173 2377 2579
Fragments arranged by size:
2579 2377 1247 173
Bpu10I CC'TnA_GC
Cuts at: 2408 3312 2408
Size: 904 5472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4501 5366 5664 4501
Size: 865 298 5213
Fragments arranged by size:
5213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 882
Size: 617 3540 2219
Fragments arranged by size:
3540 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2163 2905 4183
Size: 53 83 186 936 540 90 742 1278
Cuts at: 4183 4565 5200 275
Size: 382 635 1451
Fragments arranged by size:
1451 1278 936 742 635 540 382 186
90 83 53
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6151
Fragments arranged by size:
6151 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2075 2093 2096 2099 2306 3347 1186
Size: 877 12 18 3 3 207 1041 4215
Fragments arranged by size:
4215 1041 877 207 18 12 3 3
BseYI C'CCAG_C
Cuts at: 2158 3120 5579 6075 2158
Size: 962 2459 496 2459
Fragments arranged by size:
2459 2459 962 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2074 3389 1163
Size: 573 338 1315 4150
Fragments arranged by size:
4150 1315 573 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2322 3826 4587 5615 1540
Size: 275 186 321 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 321 275 186
BsiHKAI G_wGCw'C
Cuts at: 2316 3300 3519 4437 4522 5593 2316
Size: 984 219 918 85 1071 3099
Fragments arranged by size:
3099 1071 984 918 219 85
BsmI GAATG_Cn'
Cuts at: 3508 3887 3980 3508
Size: 379 93 5904
Fragments arranged by size:
5904 379 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2192 2192
Size: 6376
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2232 2316 3300 3519 3695 4437
Size: 399 364 78 84 984 219 176 742
Cuts at: 4437 4522 5593 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 984 742 399 364 219 176
85 84 78
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3731 844
Size: 2887 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 4263 1394
Size: 429 353 2087 3507
Fragments arranged by size:
3507 2087 429 353
BsrDI GCAATG_nn'
Cuts at: 65 1248 4871 5045 65
Size: 1183 3623 174 1396
Fragments arranged by size:
3623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5020 5207 1700
Size: 110 3210 187 2869
Fragments arranged by size:
3210 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3423 96
Size: 3327 3049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3209 3286 794
Size: 2415 77 3884
Fragments arranged by size:
3884 2415 77
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstXI CCAn_nnnn'nTGG
Cuts at: 2665 2665
Size: 6376
BstYI r'GATC_y
Cuts at: 1972 2125 2565 3152 3493 3652 3706 4073 4458
Size: 153 440 587 341 159 54 367 385
Cuts at: 4458 4475 5927 6013 6025 6371 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 587 440 385 367 346 341
159 153 86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3137 513
Size: 552 939 1133 3752
Fragments arranged by size:
3752 1133 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5637
Fragments arranged by size:
5637 683 56
BtsI GCAGTG_nn'
Cuts at: 2062 2639 3892 4686 4706 2062
Size: 577 1253 794 20 3732
Fragments arranged by size:
3732 1253 794 577 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2402 2437 529
Size: 35 1838 35 4468
Fragments arranged by size:
4468 1838 35 35
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DraIII CAC_nnn'GTG
Cuts at: 2344 2344
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 3823 4712
Size: 54 1425 50 275 18 489 1504 889
Cuts at: 4712 6341 8
Size: 1629 43
Fragments arranged by size:
1629 1504 1425 889 489 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2319 3823 1537
Size: 275 507 1504 4090
Fragments arranged by size:
4090 1504 507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4306 1353
Size: 2953 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4963 5337 5483 387
Size: 1064 138 3374 374 146 1280
Fragments arranged by size:
3374 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2486 2659 3398 4439 5036 5823 6091
Size: 393 854 173 739 1041 597 787 268
Cuts at: 6091 1239
Size: 1524
Fragments arranged by size:
1524 1041 854 787 739 597 393 268
173
EcoNI CCTnn'n_nnAGG
Cuts at: 3380 3380
Size: 6376
EcoO109I rG'GnC_Cy
Cuts at: 2228 2228
Size: 6376
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
EcoRV GAT'ATC
Cuts at: 2716 2716
Size: 6376
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2076 2223 2908 5523 1678
Size: 398 147 685 2615 2531
Fragments arranged by size:
2615 2531 685 398 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5017 5049 5091 5123 1373
Size: 32 135 32 3445 32 42 32 2626
Fragments arranged by size:
3445 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 3608 3720 3966 669
Size: 988 281 427 1243 112 246 3079
Fragments arranged by size:
3079 1243 988 427 281 246 112
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KasI G'GCGC_C
Cuts at: 2072 2904 2072
Size: 832 5544
KpnI G_GTAC'C
Cuts at: 3247 3701 3247
Size: 454 5922
MfeI C'AATT_G
Cuts at: 2419 3007 3054 3975 2419
Size: 588 47 921 4820
Fragments arranged by size:
4820 921 588 47
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5490 5674 1305
Size: 4185 184 2007
Fragments arranged by size:
4185 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4554
Fragments arranged by size:
4554 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2709 2738 3419 3512 3633 4334 4693
Size: 648 1543 29 681 93 121 701 359
Cuts at: 4693 4852 518
Size: 159 2042
Fragments arranged by size:
2042 1543 701 681 648 359 159 121
93 29
MspA1I CmG'CkG
Cuts at: 1606 2070 3792 4469 5617 5862 1606
Size: 464 1722 677 1148 245 2120
Fragments arranged by size:
2120 1722 1148 677 464 245
NarI GG'CG_CC
Cuts at: 2073 2905 2073
Size: 832 5544
NcoI C'CATG_G
Cuts at: 513 1065 2004 3137 513
Size: 552 939 1133 3752
Fragments arranged by size:
3752 1133 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3280 3355 3632 3734 5279 1132
Size: 97 2051 75 277 102 1545 2229
Fragments arranged by size:
2229 2051 1545 277 102 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 3351 5275 3351
Size: 1924 4452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6376
PfoI T'CCnGG_A
Cuts at: 2355 3356 2355
Size: 1001 5375
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 4445 4477 5958 5990 3356
Size: 32 1057 32 1481 32 3742
Fragments arranged by size:
3742 1481 1057 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspOMI G'GGCC_C
Cuts at: 2228 2228
Size: 6376
PstI C_TGCA'G
Cuts at: 830 3240 3561 3728 830
Size: 2410 321 167 3478
Fragments arranged by size:
3478 2410 321 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
SalI G'TCGA_C
Cuts at: 1936 3606 3718 1936
Size: 1670 112 4594
Fragments arranged by size:
4594 1670 112
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 3236 3557 3724 4859 5540 5731 826
Size: 219 2191 321 167 1135 681 191 1471
Fragments arranged by size:
2191 1471 1135 681 321 219 191 167
SfoI GGC'GCC
Cuts at: 2074 2906 2074
Size: 832 5544
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 2155 3703 1872
Size: 283 1548 4545
Fragments arranged by size:
4545 1548 283
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4480 5381 5643 820
Size: 197 926 2537 901 262 1553
Fragments arranged by size:
2537 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SrfI GCCC'GGGC
Cuts at: 2155 2155
Size: 6376
SspI AAT'ATT
Cuts at: 5 52 2648 4300 5
Size: 47 2596 1652 2081
Fragments arranged by size:
2596 2081 1652 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2004 3137 3815 513
Size: 552 939 1133 678 3074
Fragments arranged by size:
3074 1133 939 678 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3112 4403 4420 4573 4758 5203 325
Size: 1255 1532 1291 17 153 185 445 1498
Fragments arranged by size:
1532 1498 1291 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3423 3577 3588
Size: 274 80 33 51 1443 1446 154 11
Cuts at: 3588 3663 4622 6334 96
Size: 75 959 1712 138
Fragments arranged by size:
1712 1446 1443 959 274 154 138 80
75 51 33 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 3338 3370 6336 6368 2036
Size: 32 1270 32 2966 32 2044
Fragments arranged by size:
2966 2044 1270 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3496 3712 3496
Size: 216 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 2153 3701 1870
Size: 283 1548 4545
Fragments arranged by size:
4545 1548 283
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SwaI XcmI